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1. 发明申请

WO2011095592A1 IN VITRO PROCESS FOR THE PREPARATION OF ANTIBODIES OF THE IGG TYPE 审中-公开
标题翻译：用于制备IGG型抗体的体外制备方法
公开(公告)号：WO2011095592A1
公开(公告)日：2011-08-11
申请号：PCT/EP2011/051659
申请日：2011-02-04
申请人： FZMB GMBH FORSCHUNGSZENTRUM FÜR MEDIZINTECHNIK UND BIOTECHNOLOGIE , LÜKE, Wolfgang , MIETHE, Peter , MÜLLER-ZAHM, Katrin , WILKE, Ingo
发明人： LÜKE, Wolfgang , MIETHE, Peter , MÜLLER-ZAHM, Katrin , WILKE, Ingo
IPC分类号： C07K16/00 , C12N5/078
CPC分类号： C12P21/00 , C07K16/00 , C07K16/081 , C07K16/1282 , C07K16/14 , C07K16/16 , C07K2317/10 , C07K2317/21
摘要： An in vitro process for the preparation of antibodies of the IgG type, comprising the steps of: i. challenging dendritic cells obtained from a donor with an antigen against which the antibodies to be prepared are directed; ii. challenging CD4+ T cells obtained from the same donor with fragments of the antigen presented by the dendritic cells from step L, with the proviso that the dendritic cells are ones essentially not secreting interferon-γ (IFN-γ) and interleukin 12 (IL-12), to generate antigen-specific CD4+ Th2 cells; iii. challenging with the antigen a B cell population obtained from the same donor and including a sufficient proportion of naive B cells; and iv. contacting the antigen-specific CD4+ Th2 cells according to ii. with at least a fraction of the B cell population from iii. to form an antigen-specific plasma cell; v. immortalizing said antigen-specific plasma cell; and vi. isolating the IgG antibodies formed by said antigen-specific plasma cell.
摘要翻译：用于制备IgG型抗体的体外方法，包括以下步骤：i。从具有待制备的抗体的抗原的供体获得的具有挑战性的树突状细胞; II。从相同供体获得的具有来自步骤L的树突细胞呈递的抗原片段的CD4 + T细胞具有挑战性，条件是树突细胞基本上不分泌干扰素（IFN-α）和白介素12（IL-12），以产生抗原特异性CD4 + Th2细胞; III。与抗原挑战从相同供体获得的B细胞群体，并包括足够比例的幼稚B细胞; 和iv。根据ii。接触抗原特异性CD4 + Th2细胞。其中至少一部分来自iii的B细胞群体。以形成抗原特异性浆细胞; v永生化所述抗原特异性浆细胞; 和vi。分离由所述抗原特异性浆细胞形成的IgG抗体。

2. 发明公开

EP2531525A1 IN VITRO PROCESS FOR THE PREPARATION OF ANTIBODIES OF THE IGG TYPE 审中-公开
标题翻译：维多利亚州ZER HERSTELLUNG VONANTIKÖRPERNDES IGG-TYPS
公开(公告)号：EP2531525A1
公开(公告)日：2012-12-12
申请号：EP11702827.4
申请日：2011-02-04
申请人： FZMB GmbH Forschungszentrum für Medizintechnik und Biotechnologie
发明人： LÜKE, Wolfgang , MIETHE, Peter , MÜLLER-ZAHM, Katrin , WILKE, Ingo
IPC分类号： C07K16/00 , C12N5/078
CPC分类号： C12P21/00 , C07K16/00 , C07K16/081 , C07K16/1282 , C07K16/14 , C07K16/16 , C07K2317/10 , C07K2317/21
摘要： An in vitro process for the preparation of antibodies of the IgG type, comprising the steps of: i. challenging dendritic cells obtained from a donor with an antigen against which the antibodies to be prepared are directed; ii. challenging CD4+ T cells obtained from the same donor with fragments of the antigen presented by the dendritic cells from step L, with the proviso that the dendritic cells are ones essentially not secreting interferon-γ (IFN-γ) and interleukin 12 (IL-12), to generate antigen-specific CD4+ Th2 cells; iii. challenging with the antigen a B cell population obtained from the same donor and including a sufficient proportion of naive B cells; and iv. contacting the antigen-specific CD4+ Th2 cells according to ii. with at least a fraction of the B cell population from iii. to form an antigen-specific plasma cell; v. immortalizing said antigen-specific plasma cell; and vi. isolating the IgG antibodies formed by said antigen-specific plasma cell.
摘要翻译：用于制备IgG型抗体的体外方法，包括以下步骤：i。从具有待制备抗体的抗原的供体获得的具有挑战性的树突状细胞; II。挑选来自与来自步骤L的树突状细胞的抗原片段相同供体的CD4 + T细胞，条件是树突状细胞基本上不分泌干扰素-γ（IFN-γ）和白细胞介素12（IL-12）），以产生抗原特异性CD4 + Th2细胞; III。从抗原挑选从相同供体获得的B细胞群，并包括足够比例的幼稚B细胞; 和iv。根据ii。接触抗原特异性CD4 + Th2细胞。至少有一部分来自iii的B细胞群体。以形成抗原特异性浆细胞; v。永生化所述抗原特异性浆细胞; 和vi。分离由所述抗原特异性浆细胞形成的IgG抗体。

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