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1. 发明申请

WO02081935A8 MICROFABRICATED FLUIDIC CIRCUIT ELEMENTS AND APPLICATIONS 审中-公开
标题翻译：微流控流体元件及应用
公开(公告)号：WO02081935A8
公开(公告)日：2004-10-21
申请号：PCT/US0145294
申请日：2001-11-30
申请人： FLUIDIGM CORP , FERNANDES DAVID N , CHOU HOU-PU , UNGER MARC A
发明人： FERNANDES DAVID N , CHOU HOU-PU , UNGER MARC A
IPC分类号： B01L3/00 , F15C3/04 , F15C5/00 , F16K99/00 , G06F17/50
CPC分类号： F15C3/00 , A61M2206/22 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2200/0621 , B01L2200/14 , B01L2300/123 , B01L2400/0487 , B01L2400/0638 , B01L2400/0655 , F15C3/04 , F16K99/0001 , F16K99/0009 , F16K99/0015 , F16K99/0034 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0082 , F16K2099/0084 , G06F17/509 , G06F2217/16 , H01H2029/008 , Y10T137/206 , Y10T137/2202 , Y10T137/2218 , Y10T137/2224 , Y10T137/7879 , Y10T137/7891 , Y10T137/7892
摘要： The present invention provides microfabricated fluidic systems and methods. Microfabricated fluidic devices of the present invention include switches (232, 233) that can be opened and closed to allow or block the flow of fluid through a channel in response to the pressure level in a gate of the switch. The microfabricated fluidic switches may be coupled together to perform logic functions and Boolean algebra, such as inverters, AND gates, NAND, gates, NOR gates, and OR gates. The logic gates may be coupled together to form flip-flops that latch signals. The present invention also includes microfabricated fluidic pressure multipliers that increase the pressure (P2) in a second chamber relative to a first chamber. Microfabricated fluidic devices of the present invention also include pressure sources (560). A pressure source of the present includes a pump coupled to a reservoir through unidirectional valves (500). The pressure source may be high pressure source or a low pressure source. Microfabricated fluidic devices of the present invention may also include devices that perform analog functions such as switching regulator.
摘要翻译：本发明提供微制造流体系统和方法。本发明的微型流体装置包括可以打开和关闭的开关（232,233），以响应于开关的门中的压力水平来允许或阻止流体流过通道的流动。微制造的流体开关可以耦合在一起以执行逻辑功能和布尔代数，例如反相器，与门，NAND，门，NOR门和或门。逻辑门可以耦合在一起以形成锁存信号的触发器。本发明还包括相对于第一腔室增加第二腔室中的压力（P2）的微制式流体压力倍增器。本发明的微型流体装置还包括压力源（560）。本发明的压力源包括通过单向阀（500）联接到储存器的泵。压力源可以是高压源或低压源。本发明的微型流体装置还可以包括执行诸如开关调节器的模拟功能的装置。

2. 发明申请

WO02060582A2 MICROFLUIDIC DEVICES FOR INTRODUCING AND DISPENSING FLUIDS FROM MICROFLUIDIC SYSTEMS 审中-公开
标题翻译：用于从微流体系统引入和分配流体的微流体装置
公开(公告)号：WO02060582A2
公开(公告)日：2002-08-08
申请号：PCT/US0145256
申请日：2001-11-16
申请人： FLUIDIGM CORP , CHOU HOU-PU , MANGER IAN DAVID , UNGER MARC A , YI YONG , FERNANDEZ DAVE
发明人： CHOU HOU-PU , MANGER IAN DAVID , UNGER MARC A , YI YONG , FERNANDEZ DAVE
IPC分类号： B01L3/00 , B01L3/02 , B29C65/00 , B29C65/48 , B81B1/00 , F15C5/00 , F16K99/00 , G01N1/00 , B01L
CPC分类号： B01L3/02 , B01L3/0268 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L2200/027 , B01L2200/0605 , B01L2300/0816 , B01L2300/0867 , B01L2300/0887 , B01L2300/123 , B01L2400/0406 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0688 , B29C65/006 , B29C65/48 , B29C66/112 , B29C66/1122 , B29C66/114 , B29C66/1222 , B29C66/1224 , B29C66/1226 , B29C66/532 , B29C66/54 , B29C66/71 , B29L2031/756 , B81B2201/13 , F16K99/0001 , F16K99/0015 , F16K99/0017 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0084 , G01N1/14 , Y10T137/0318 , Y10T137/0396 , Y10T436/11 , Y10T436/118339 , Y10T436/119163 , B29K2021/00
摘要： The present invention provides microfluidic devices, systems and methods for using the same, which facilitate the introduction of fluid to and from a microfluidic channel located within the microfluidic devices.
摘要翻译：本发明提供了使用该流体装置的微流体装置，系统和方法，其有助于将流体引入到位于微流体装置内的微流体通道和从微流体通道中引入流体。

3. 发明申请

WO2005107938A3 THERMAL REACTION DEVICE AND METHOD FOR USING THE SAME 审中-公开
标题翻译：热反应装置及其使用方法
公开(公告)号：WO2005107938A3
公开(公告)日：2006-01-12
申请号：PCT/US2005015352
申请日：2005-05-02
申请人： FLUIDIGM CORP , GOODSAID FEDERICO , UNGER MARC A , HUANG JIANG , QUAN EMERSON , GROSSMAN ROBERT , LAM PHILLIP , CHOU HOU-PU , KIMBALL JAKE , PIEPRZYK MARTIN , FACER GEOFFREY
发明人： GOODSAID FEDERICO , UNGER MARC A , HUANG JIANG , QUAN EMERSON , GROSSMAN ROBERT , LAM PHILLIP , CHOU HOU-PU , KIMBALL JAKE , PIEPRZYK MARTIN , FACER GEOFFREY
IPC分类号： B01J3/00 , B01J4/00 , B01J10/00 , B01J19/00 , B01L3/00 , B01L7/00 , B01L9/00 , B01L3/02 , G01N15/06 , G01N21/00 , G01N31/22
CPC分类号： B01L7/52 , B01J19/0046 , B01J2219/00353 , B01J2219/004 , B01J2219/00403 , B01J2219/00495 , B01J2219/00576 , B01J2219/00587 , B01J2219/0059 , B01J2219/0072 , B01J2219/00722 , B01L3/502707 , B01L3/502738 , B01L9/527 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2300/0816 , B01L2300/0887 , B01L2300/1805 , B01L2400/0481 , B01L2400/0638
摘要： An M x N matrix microfluidic device for performing a matrix of reactions, the device (100) having a plurality of reaction cells (106) in communication with one of either a sample inlet (120) or a reagent t inlet (124) through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method includes using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
摘要翻译：一种用于执行反应矩阵的M×N矩阵微流体装置，所述装置（100）具有多个反应池（106），其通过样品入口（120）或试剂t入口（124）中的一个通过通过形成在装置的弹性体块内。提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。

4. 发明申请

WO02060582A9 MICROFLUIDIC DEVICES FOR INTRODUCING AND DISPENSING FLUIDS FROM MICROFLUIDIC SYSTEMS 审中-公开
标题翻译：用于从微流体系统引入和分配流体的微流体装置
公开(公告)号：WO02060582A9
公开(公告)日：2003-05-30
申请号：PCT/US0145256
申请日：2001-11-16
申请人： FLUIDIGM CORP , CHOU HOU-PU , MANGER IAN DAVID , UNGER MARC A , YI YONG , FERNANDEZ DAVE
发明人： CHOU HOU-PU , MANGER IAN DAVID , UNGER MARC A , YI YONG , FERNANDEZ DAVE
IPC分类号： B01L3/00 , B01L3/02 , B29C65/00 , B29C65/48 , B81B1/00 , F15C5/00 , F16K99/00 , G01N1/00
CPC分类号： B01L3/02 , B01L3/0268 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L2200/027 , B01L2200/0605 , B01L2300/0816 , B01L2300/0867 , B01L2300/0887 , B01L2300/123 , B01L2400/0406 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0688 , B29C65/006 , B29C65/48 , B29C66/112 , B29C66/1122 , B29C66/114 , B29C66/1222 , B29C66/1224 , B29C66/1226 , B29C66/532 , B29C66/54 , B29C66/71 , B29L2031/756 , B81B2201/13 , F16K99/0001 , F16K99/0015 , F16K99/0017 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0084 , G01N1/14 , Y10T137/0318 , Y10T137/0396 , Y10T436/11 , Y10T436/118339 , Y10T436/119163 , B29K2021/00
摘要： The present invention provides microfluidic devices, systems and methods for using the same, which facilitate the introduction of fluid to and from a microfluidic channel located within the microfluidic devices.
摘要翻译：本发明提供微流体装置，使用其的微流体装置，系统和方法，其有利于将流体引入微流体装置内的微流体通道以及从微流体通道中引出。

5. 发明申请

WO0101025A9 MICROFABRICATED ELASTOMERIC VALVE AND PUMP SYSTEMS 审中-公开
标题翻译：微型弹药阀和泵系统
公开(公告)号：WO0101025A9
公开(公告)日：2002-07-25
申请号：PCT/US0017740
申请日：2000-06-27
申请人： CALIFORNIA INST OF TECHN , UNGER MARC A , CHOU HOU-PU , THORSEN TODD A , SCHERER AXEL , QUAKE STEPHEN R
发明人： UNGER MARC A , CHOU HOU-PU , THORSEN TODD A , SCHERER AXEL , QUAKE STEPHEN R
IPC分类号： G03F7/20 , B01L3/00 , B81B1/00 , B81B3/00 , B81B7/00 , B81C1/00 , B81C99/00 , C12Q1/68 , F04B17/00 , F04B35/04 , F04B43/04 , F15C5/00 , F16K7/00 , F16K17/02 , F16K99/00
CPC分类号： B29C51/00 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B33Y80/00 , B81B5/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , B81C2201/034 , C12Q1/6874 , F04B43/043 , F15C1/06 , F15C5/00 , F16K11/20 , F16K13/00 , F16K31/126 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/0491 , Y10T137/0497 , Y10T137/2224 , Y10T137/87249 , Y10T156/10 , Y10T428/24479 , Y10T428/24744 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising : forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
摘要翻译：一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

6. 发明申请

WO0101025A2 MICROFABRICATED ELASTOMERIC VALVE AND PUMP SYSTEMS 审中-公开
标题翻译： MICROFABRICATED ELASTOMERIC阀和泵系统
公开(公告)号：WO0101025A2
公开(公告)日：2001-01-04
申请号：PCT/US0017740
申请日：2000-06-27
申请人： CALIFORNIA INST OF TECHN , UNGER MARC A , CHOU HOU PU , THORSEN TODD A , SCHERER AXEL , QUAKE STEPHEN R
发明人： UNGER MARC A , CHOU HOU-PU , THORSEN TODD A , SCHERER AXEL , QUAKE STEPHEN R
IPC分类号： G03F7/20 , B01L3/00 , B81B1/00 , B81B3/00 , B81B7/00 , B81C1/00 , B81C99/00 , C12Q1/68 , F04B17/00 , F04B35/04 , F04B43/04 , F15C5/00 , F16K7/00 , F16K17/02 , F16K99/00 , F16K17/00
CPC分类号： B29C51/00 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B33Y80/00 , B81B5/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , B81C2201/034 , C12Q1/6874 , F04B43/043 , F15C1/06 , F15C5/00 , F16K11/20 , F16K13/00 , F16K31/126 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/0491 , Y10T137/0497 , Y10T137/2224 , Y10T137/87249 , Y10T156/10 , Y10T428/24479 , Y10T428/24744 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising : forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
摘要翻译：一种制造弹性体结构的方法，包括：在第一微机械模具的顶部上形成第一弹性体层，所述第一微机械模具具有第一凸起突起，所述第一凸起形成沿所述第一弹性体层的底表面延伸的第一凹陷; 在第二微机械模具的顶部上形成第二弹性体层，所述第二微机械模具具有第二凸起突起，所述第二凸起形成沿着所述第二弹性体层的底表面延伸的第二凹陷; 将所述第二弹性体层的底表面结合到所述第一弹性体层的顶表面上，使得在所述第一和第二弹性体层之间的所述第二凹陷中形成控制通道; 以及将第一弹性体层定位在平面基底的顶部上，使得在第一弹性体层和平面基底之间的第一凹陷中形成流动通道。

7. 发明申请

WO2005072353A3 CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME 审中-公开
标题翻译：晶体形成装置和系统及其制造和使用方法
公开(公告)号：WO2005072353A3
公开(公告)日：2007-02-22
申请号：PCT/US2005002408
申请日：2005-01-25
申请人： FLUIDIGM CORP , GROSSMAN ROBERT , UNGER MARC , LAM PHILLIP , CHOU HOU-PU , KIMBALL JAKE , PIEPRZYK MARTIN
发明人： GROSSMAN ROBERT , UNGER MARC , LAM PHILLIP , CHOU HOU-PU , KIMBALL JAKE , PIEPRZYK MARTIN
IPC分类号： G01N15/06 , B01J19/00 , B01L3/00 , B01L3/06 , B01L9/00 , B01L99/00 , C30B7/00 , C30B29/58 , G01N33/00 , G01N33/48
CPC分类号： C12Q1/68 , B01L3/0293 , B01L3/06 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/5635 , B01L3/565 , B01L9/527 , B01L2200/027 , B01L2200/0605 , B01L2300/0816 , B01L2300/14 , B01L2400/0487 , B01L2400/0655 , C30B7/00 , C30B29/58 , Y10T436/11
摘要： The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.
摘要翻译：本发明提供了微流体装置及其使用方法。本发明还提供了用于使用微流体装置的装置和系统，分析在微流体装置中进行的反应，以及用于生成，存储，组织和分析使用微流体装置产生的数据的系统。本发明还提供了使用和制造微流体系统和装置的方法，在一些实施方案中，它们可用于晶体形成。在一个实施例中，一种装置包括具有其中具有一个或多个流体端口的压板面的压板。流体端口在空间上对应于微流体装置表面上的一个或多个孔。包括用于相对于压板保持微流体装置的平台，以及用于将压板压靠在微流体装置上的压板致动器，使得压板的至少一个流体端口被推压到一个井中以形成压力室包括井和端口，使得当压力流体通过其中一个端口被引入或从压力室移除时，流体压力在其中改变。

8. 发明申请

WO2007033385A3 MICROFLUIDIC ASSAY DEVICES AND METHODS 审中-公开
标题翻译：微流感测定装置及方法
公开(公告)号：WO2007033385A3
公开(公告)日：2007-07-12
申请号：PCT/US2006036365
申请日：2006-09-13
申请人： FLUIDIGM CORP , NASSEF HANY RAMEZ , CHOU HOU-PU , LUCERO MICHAEL , MAY ANDREW , YOKOBATA KATHY
发明人： NASSEF HANY RAMEZ , CHOU HOU-PU , LUCERO MICHAEL , MAY ANDREW , YOKOBATA KATHY
IPC分类号： G01N33/543 , B01L3/00
CPC分类号： G01N33/54366 , B01J2219/00286 , B01J2219/00317 , B01J2219/00389 , B01J2219/00398 , B01L3/5025 , B01L3/502715 , B01L3/502738 , B01L2300/0816 , B01L2300/0874 , B01L2300/123 , B01L2400/0487 , B01L2400/0655 , F16K99/0001 , F16K99/0015 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0084 , G01N33/5302
摘要： A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.
摘要翻译：微流体装置适于执行许多同时结合测定，包括但不限于免疫学实验，例如ELISA测定，初级和次级抗体之间的最小串扰。

9. 发明申请

WO0175176A3 METHODS AND SYSTEMS FOR MOLECULAR FINGERPRINTING 审中-公开
标题翻译：用于分子指纹的方法和系统
公开(公告)号：WO0175176A3
公开(公告)日：2003-08-07
申请号：PCT/US0110881
申请日：2001-04-04
申请人： CALIFORNIA INST OF TECHN , QUAKE STEPHEN R , CHOU HOU-PU
发明人： QUAKE STEPHEN R , CHOU HOU-PU
IPC分类号： C12Q1/68 , B01L3/00
CPC分类号： C12Q1/683
摘要： This invention relates in general to a method for molecular fingerprinting. The method can be used for forensic identification (e.g. DNA fingerprinting, especially by VNTR), bacteria typing, and human/animal pathogen diagnosis. More particularly, molecules such as polynucleotides (e.g. DNA) can be assessed or sorted by size in a microfabricated device that analyzes the polynucleotides according to restriction fragment length polymorphism. In a microfabricated device according to the invention, DNA fragments or other molecules can be rapidly and accurately typed using relatively small samples, by measuring for example the signal of an optically-detectable (e.g., fluorescent) reporter associated with the polynucleotide fragments.
摘要翻译：本发明一般涉及分子指纹图谱的方法。该方法可用于法医鉴定（例如DNA指纹图谱，特别是VNTR），细菌分型和人/动物病原体诊断。更具体地，可以按照限制性片段长度多态性分析多核苷酸的微制造装置中的大小来评估或分类分子，例如多核苷酸（例如DNA）。在根据本发明的微制造装置中，通过测量例如与多核苷酸片段相关的可光学检测（例如，荧光）报告基因的信号，可以使用相对小的样品快速和精确地分类DNA片段或其他分子。

10. 发明申请

WO0194635A9 INTEGRATED ACTIVE FLUX MICROFLUIDIC DEVICES AND METHODS 审中-公开
标题翻译：集成有源通量微流体器件和方法
公开(公告)号：WO0194635A9
公开(公告)日：2002-08-08
申请号：PCT/US0118400
申请日：2001-06-05
申请人： CALIFORNIA INST OF TECHN , QUAKE STEPHEN R , CHOU HOU-PU
发明人： QUAKE STEPHEN R , CHOU HOU-PU
IPC分类号： G01N21/64 , B01F5/06 , B01F13/00 , B01F15/02 , B01L3/00 , B01L99/00 , B81B1/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N21/05 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/58 , G01N37/00 , B01J19/00
CPC分类号： B01L3/02 , B01F5/0646 , B01F5/0647 , B01F13/0072 , B01F13/0093 , B01F15/0201 , B01F15/024 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2200/0689 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2300/0867 , B01L2300/088 , B01L2300/0887 , B01L2300/123 , B01L2400/0418 , B01L2400/043 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , B01L2400/0694 , C12M1/02 , C12Q1/6816 , G01N33/53 , Y10T137/0318 , Y10T137/0329 , Y10T137/2218 , Y10T137/2224 , Y10T436/11 , Y10T436/145555 , Y10T436/173845 , Y10T436/2575 , C12Q2565/629
摘要： The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.
摘要翻译：本发明涉及用于快速检测与特定疾病相关的DNA，蛋白质或其他分子的微制造装置。本发明的装置和方法可以用于通过检测分子（例如分子数量），例如多核苷酸（例如DNA）或蛋白质（例如抗体），通过测量可检测信号来同时诊断多种疾病报道子与杂交的多核苷酸或抗原/抗体复合物相关。在根据本发明的微制造装置中，检测分子（即多核苷酸，蛋白质或抗原/抗体复合物）的存在与来自与结合分子相关的光学可检测（例如荧光）报道分子的杂交信号相关。这些杂交信号可以通过任何合适的手段检测，例如光学检测，并且可以例如作为特定基因存在的表示存储在计算机中。可以将杂交探针固定在形成一部分或暴露于形成闭合环路的装置的一个或多个通道的基底上，以使样品循环以主动接触互补探针。根据本发明的通用芯片不仅可以用DNA制造，而且可以用其他分子如RNA，蛋白质，肽核酸（PNA）和聚酰胺分子制造。

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