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1. 发明申请

WO2011128377A1 METHOD FOR THE SELECTION OF A LONG-TERM PRODUCING CELL 审中-公开
标题翻译：选择长期生产细胞的方法
公开(公告)号：WO2011128377A1
公开(公告)日：2011-10-20
申请号：PCT/EP2011/055835
申请日：2011-04-13
申请人： F. Hoffmann-La Roche AG , GOEPFERT, Ulrich , OSTERLEHNER, Andrea , SIMMETH, Silke
发明人： GOEPFERT, Ulrich , OSTERLEHNER, Andrea , SIMMETH, Silke
IPC分类号： C12Q1/68 , C07K16/00 , C12P21/02
CPC分类号： C12Q1/6888 , C07K16/00 , C12P21/00 , C12P21/02 , C12Q1/6827 , C12Q1/6881 , C12Q2600/154
摘要： Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide.
摘要翻译：本文报道了用于确定与编码多肽的核酸可操作地连接的启动子核酸甲基化的方法，从而确定细胞的长期生产力。另外一个方面是通过确定可操作地连接到编码多肽的结构基因的启动子核酸的甲基化来选择用于产生多肽的细胞的方法。

2. 发明申请

WO2010046094A1 DETERMINATION OF IMMUNOGLOBULIN ENCODING NUCLEID ACID 审中-公开
标题翻译：免疫球蛋白编码核酸的测定
公开(公告)号：WO2010046094A1
公开(公告)日：2010-04-29
申请号：PCT/EP2009/007521
申请日：2009-10-21
申请人： F. HOFFMANN-LA ROCHE AG , GOEPFERT, Ulrich , JENTZSCH, Marlene , OSTERLEHNER, Andrea
发明人： GOEPFERT, Ulrich , JENTZSCH, Marlene , OSTERLEHNER, Andrea
IPC分类号： C12Q1/68 , C07K16/00 , C12P21/00
CPC分类号： C12Q1/6876 , C12Q2600/158 , C12Q2600/16
摘要： It is reported herein a method for the determination of the amount of immunoglobulin-encoding mRNA comprising: a) providing a sample, b) performing a polymerase chain reaction for amplifying the light chain with the primers of SEQ ID NO: 23 and 24 and the probe of SEQ ID NO: 33, and/or c) performing a polymerase chain reaction for amplifying the heavy chain with the primers of SEQ ID NO: 19 and 21 and the probe of SEQ ID NO: 40, and d) quantitating with an efficiency of 2.0. The primers with SEQ ID NOs 23 and 24 bind at positions CL 247-266 and CL166-185, respectively, and the probe with SEQ ID NO: 33 binds at 189-212 in human IgG koppa chain. The primer with SEQ ID NO: 19 binds at CH region 2 position 220-237 and the primer with SEQ ID NO: 21 binds at CH region 3 position 114-133. Finally the probe with SEQ ID NO: 40 binds from position 315 in CH2 to position 7 in CH3.
摘要翻译：本文报道了用于测定免疫球蛋白编码mRNA的量的方法，其包括：a）提供样品，b）用引物SEQ ID NO：23和24进行用于扩增轻链的聚合酶链式反应， SEQ ID NO：33的探针，和/或c）使用SEQ ID NO：19和21的引物和SEQ ID NO：40的探针进行用于扩增重链的聚合酶链式反应，以及d）用效率2.0。具有SEQ ID NO 23和24的引物分别在CL 247-266和CL166-185的位置结合，并且具有SEQ ID NO：33的探针在人IgG koppa链中的189-212结合。具有SEQ ID NO：19的引物在CH区域2位置220-237处结合，并且具有SEQ ID NO：21的引物在第3区114-133位结合。最后，具有SEQ ID NO：40的探针从CH2中的位置315与CH3中的位置7结合。

3. 发明申请

WO2018162517A1 METHOD FOR PRODUCING MULTISPECIFIC ANTIBODIES 审中-公开
公开(公告)号：WO2018162517A1
公开(公告)日：2018-09-13
申请号：PCT/EP2018/055532
申请日：2018-03-07
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： SEEBER, Stefan , GOEPFERT, Ulrich , OSTERLEHNER, Andrea , KETTENBERGER, Hubert , PAUL, Wolfgang
IPC分类号： C07K16/00 , C07K16/46 , C12N15/67 , C12N15/85
摘要： Herein are provided a method for producing a multispecific antibody comprising the steps of providing a mammalian cell expressing the antibody, transfecting said mammalian cell with an expression vector comprising an expression cassette encoding a polypeptide of the antibody that has a domain crossover, cultivating the transfected cell and recovering the antibody from the cell or the cultivation medium and thereby producing the multispecific antibody.

4. 发明公开

EP3592767A1 METHOD FOR PRODUCING MULTISPECIFIC ANTIBODIES 审中-公开
公开(公告)号：EP3592767A1
公开(公告)日：2020-01-15
申请号：EP18710013.6
申请日：2018-03-07
申请人： F. Hoffmann-La Roche AG
发明人： SEEBER, Stefan , GOEPFERT, Ulrich , OSTERLEHNER, Andrea , KETTENBERGER, Hubert , PAUL, Wolfgang
IPC分类号： C07K16/00 , C07K16/46 , C12N15/67 , C12N15/85

5. 发明公开

EP2340317A1 DETERMINATION OF IMMUNOGLOBULIN ENCODING NUCLEID ACID 审中-公开
标题翻译：确定免疫球蛋白编码核酸
公开(公告)号：EP2340317A1
公开(公告)日：2011-07-06
申请号：EP09740273.9
申请日：2009-10-21
申请人： F. Hoffmann-La Roche AG
发明人： GOEPFERT, Ulrich , JENTZSCH, Marlene , OSTERLEHNER, Andrea
IPC分类号： C12Q1/68 , C07K16/00 , C12P21/00
CPC分类号： C12Q1/6876 , C12Q2600/158 , C12Q2600/16
摘要： It is reported herein a method for the determination of the amount of immunoglobulin-encoding mRNA comprising: a) providing a sample, b) performing a polymerase chain reaction for amplifying the light chain with the primers of SEQ ID NO: 23 and 24 and the probe of SEQ ID NO: 33, and/or c) performing a polymerase chain reaction for amplifying the heavy chain with the primers of SEQ ID NO: 19 and 21 and the probe of SEQ ID NO: 40, and d) quantitating with an efficiency of 2.0. The primers with SEQ ID NOs 23 and 24 bind at positions CL 247-266 and CL166-185, respectively, and the probe with SEQ ID NO: 33 binds at 189-212 in human IgG koppa chain. The primer with SEQ ID NO: 19 binds at CH region 2 position 220-237 and the primer with SEQ ID NO: 21 binds at CH region 3 position 114-133. Finally the probe with SEQ ID NO: 40 binds from position 315 in CH2 to position 7 in CH3.

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