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1. 发明申请

WO2013076139A1 CD40L EXPRESSING MAMMALIAN CELLS AND THEIR USE 审中-公开
标题翻译： CD40L表达MAMMALIAN细胞及其用途
公开(公告)号：WO2013076139A1
公开(公告)日：2013-05-30
申请号：PCT/EP2012/073232
申请日：2012-11-21
申请人： F. HOFFMANN-LA ROCHE AG , ENDL, Josef , FISCHER, Jens , KERN, Peter , OFFNER, Sonja , PLATZER, Josef , SEEBER, Stefan
发明人： ENDL, Josef , FISCHER, Jens , KERN, Peter , OFFNER, Sonja , PLATZER, Josef , SEEBER, Stefan
IPC分类号： C07K16/00 , C12N5/16 , C12N5/0781
CPC分类号： C07K16/28 , C07K16/00 , C07K2317/14 , C12N5/0635 , C12N2501/2302 , C12N2501/2321 , C12N2501/52 , C12N2502/99 , C12N2510/00
摘要： Herein is reported a co-cultivation system for co-cultivating a pool of rabbit B-cells or single deposited rabbit B-cells wherein cells CD40L expressing CHO cells are used as feeder in the presence of IL-2 and IL-21.
摘要翻译：本文报道了用于共培养兔B细胞池或单沉积兔B细胞的共培养系统，其中在IL-2和IL-21存在下，使用表达CD40L的CHO细胞作为进料器。

2. 发明申请

WO2011147903A1 SINGLE B-CELL CULTIVATION METHOD 审中-公开
标题翻译：单B细胞培养方法
公开(公告)号：WO2011147903A1
公开(公告)日：2011-12-01
申请号：PCT/EP2011/058616
申请日：2011-05-26
申请人： F. HOFFMANN-LA ROCHE AG , ENDL, Josef , HERR, Natalie , OFFNER, Sonja , PLATZER, Josef , SIEWE, Basile , THOREY, Irmgard
发明人： ENDL, Josef , HERR, Natalie , OFFNER, Sonja , PLATZER, Josef , SIEWE, Basile , THOREY, Irmgard
IPC分类号： G01N33/50
CPC分类号： C12N5/0635 , C07K16/00 , C12N2500/30 , C12N2500/32 , C12N2500/60 , C12N2500/72 , C12N2501/20 , C12N2501/2301 , C12N2501/2302 , C12N2501/231 , C12N2501/2321 , C12N2501/24 , C12N2501/25 , C12N2502/1114 , C12N2502/1157 , C12N2502/1185 , C12N2502/70 , G01N15/14 , G01N2015/008 , G01N2015/149
摘要： Herein is reported a method for obtaining a B-cell comprising the following steps a) labeling B-cells, b) depositing the labeled B-cells as single cells, c) co- cultivating the single cell deposited B-cells with feeder cells, d) selecting a B-cell proliferating and secreting IgG in step c) and thereby obtaining a B-cell. The labeling can be of IgG + CD19 + -B-cells, IgG + CD38 + -B-cells, IgG + CD268 + -B-cells, IgG - CD138 + -B-cells, CD27 + CD138 + -B-cells or CD3 - CD27 + -B-cells. The method can comprise the step of incubating said B-cells at 37 °C for one hour in EL-4 B5 medium prior to the depositing step. The method can also comprise the step of centrifuging said single cell deposited B-cells prior to the co-cultivation. In the co- cultivation a feeder mix comprising interleukin-lbeta, and tumor necrosis factor alpha and Staphylococcus aureus strain Cowans cells or BAFF or interleukin-2 and/or interleukin-10 and/or interleukin-6 and/or interleukin-4 can be used.
摘要翻译：本文报道了一种获得B细胞的方法，包括以下步骤：a）标记B细胞，b）将标记的B细胞沉积为单细胞，c）使单细胞沉积的B细胞与饲养细胞共培养， d）在步骤c）中选择B细胞增殖和分泌IgG，从而获得B细胞。标记可以是IgG + CD19 + -B细胞，IgG + CD38 + -B细胞，IgG + CD268 + -B细胞，IgG-CD138 + -B细胞，CD27 + CD138 + -B细胞或CD3-CD27 + -B -细胞。该方法可以包括在沉积步骤之前在37℃下在EL-4B5培养基中孵育所述B细胞1小时的步骤。该方法还可以包括在共培养之前将所述单细胞沉积的B细胞离心的步骤。在共培养中，包含白细胞介素-Ibeta和肿瘤坏死因子α和金黄色葡萄球菌菌株Cowans细胞或BAFF或白细胞介素-2和/或白细胞介素-10和/或白细胞介素-6和/或白细胞介素-4的饲养组合物可以是用过的。

3. 发明申请

WO2012113813A1 ANTIBODIES AGAINST HUMAN IL33R AND USES THEREOF 审中-公开
标题翻译：抗体对人IL33R的抗体及其用途
公开(公告)号：WO2012113813A1
公开(公告)日：2012-08-30
申请号：PCT/EP2012/052979
申请日：2012-02-22
申请人： F. HOFFMANN-LA ROCHE AG , FERTIG, Georg , FISCHER, Jens , GEORGES, Guy , KALUZA, Klaus , LIFKE, Valeria , MOELLEKEN, Joerg , OFFNER, Sonja , PASHINE, Achal , SEEBER, Stefan
发明人： FERTIG, Georg , GEORGES, Guy , KALUZA, Klaus , LIFKE, Valeria , MOELLEKEN, Joerg , OFFNER, Sonja , PASHINE, Achal , SEEBER, Stefan
IPC分类号： C07K16/28 , A61K39/395 , A61P37/06 , A61P11/06 , A61P19/02
CPC分类号： C07K16/2866 , A61K39/395 , A61K39/39558 , A61K2039/505 , C07K2317/24 , C07K2317/30 , C07K2317/76 , C07K2317/92
摘要： An antibody binding to IL33R characterized in that the heavy chain variable domain comprises a CDR3 region of SEQ ID NO: l, a CDR2 region of SEQ ID NO:2 and a CDRl region of SEQ ID NO:3 and in that the light chain variable domain comprises a CDR3 region of SEQ ID NO:4, a CDR2 region of SEQ ID NO:5 and a CDRl region of SEQ ID NO:6 or a a chimeric, humanized or T cell epitope depleted antibody variant thereof has advantageous properties for the treatment of inflammatory diseases.
摘要翻译：结合IL33R的抗体，其特征在于重链可变结构域包含SEQ ID NO：1的CDR3区，SEQ ID NO：2的CDR2区和SEQ ID NO：3的CDR1区，并且其中轻链可变区结构域包含SEQ ID NO：4的CDR3区，SEQ ID NO：5的CDR2区和SEQ ID NO：6的CDR1区，或嵌合，人源化或T细胞表位缺失的抗体变体具有治疗的有利性质的炎性疾病。

4. 发明申请

WO2010142423A2 USE OF AN ANTI-TAU PS422 ANTIBODY FOR THE TREATMENT OF BRAIN DISEASES 审中-公开
标题翻译：使用抗谷氨酸PS422抗体治疗脑疾病
公开(公告)号：WO2010142423A2
公开(公告)日：2010-12-16
申请号：PCT/EP2010/003437
申请日：2010-06-08
申请人： F. HOFFMANN-LA ROCHE AG , BOHRMANN, Bernd , GOEPFERT, Ulrich , GRUENINGER, Fiona , HUBER, Walter , KRELL, Hans-Willi , LIFKE, Valeria , MUNDIGL, Olaf , OFFNER, Sonja , OZMEN, Laurence , SCHRAEML, Michael
发明人： BOHRMANN, Bernd , GOEPFERT, Ulrich , GRUENINGER, Fiona , HUBER, Walter , KRELL, Hans-Willi , LIFKE, Valeria , MUNDIGL, Olaf , OFFNER, Sonja , OZMEN, Laurence , SCHRAEML, Michael
IPC分类号： C07K16/18
CPC分类号： C07K16/18 , A61K2039/505 , C07K14/4711 , C07K16/3053 , C07K2317/622 , C07K2317/92 , C07K2317/94 , C07K2319/40 , C07K2319/55
摘要： An antibody binding to Tau, phosphorylated at serine 422 (pS422), characterized in specifically binding to phosphorylated Tau fragment of SEQ ID NO: 9 and to Tau pS422, but not binding to Tau and to phosphorylated MCAK fragment of SEQ ID NO: 17 is useful in the treatment of a Tauopathy.
摘要翻译：特异性结合SEQ ID NO：9的磷酸化Tau片段和Tau pS422，但不结合Tau和SEQ ID NO：17的磷酸化MCAK片段的以丝氨酸422（pS422）磷酸化的抗体结合Tau的抗体是可用于治疗Tauopathy。

5. 发明申请

WO2018210896A1 METHOD FOR THE PRODUCTION OF THYMOCYTE SUPERNATANT 审中-公开
公开(公告)号：WO2018210896A1
公开(公告)日：2018-11-22
申请号：PCT/EP2018/062647
申请日：2018-05-16
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： OFFNER, Sonja , HORVATH, Markus , SAAM, Ingried
IPC分类号： C12N5/0781
摘要： Herein is reported a method for producing a thymocyte supernatant comprising the steps of co-cultivating thymocytes and mononuclear cells at a cell ratio of at least 0.5:1.2 in the presence of phorbol-12-myristate-13-acetate and Phytohemagglutinin M for up to 60 hours, and separating the co-cultivation medium from the cells and thereby producing the thymocyte supernatant.

6. 发明申请

WO2016096788A1 ASSAY AND METHOD FOR DETERMINING CDC ELICITING ANTIBODIES 审中-公开
标题翻译：用于确定CDC抗体的测定和方法
公开(公告)号：WO2016096788A1
公开(公告)日：2016-06-23
申请号：PCT/EP2015/079693
申请日：2015-12-15
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： OFFNER, Sonja , ZICK, Karlheinz
IPC分类号： G01N33/50 , G01N33/68
CPC分类号： G01N33/6854 , G01N33/5011 , G01N2333/4716 , G01N2500/10
摘要： Herein is reported a method for determining complement dependent cytotoxicity of a composition comprising i) a first binding site that specifically binds to a first epitope on a first antigen, which is conjugated to a first Fc-region polypeptide of human origin, and ii) a second binding site that specifically binds to a second epitope on a second antigen, which is conjugated to a second Fc-region polypeptide of human origin, wherein the method comprises the steps of incubating a cell expressing the first antigen and the second antigen with the composition; adding rabbit complement to the mixture; and determining cell lysis and thereby determining complement dependent cytotoxicity of the composition.
摘要翻译：本文报道了用于测定组合物的补体依赖性细胞毒性的方法，其包括i）特异性结合第一抗原上的第一个表位的第一结合位点，其与人源的第一Fc区多肽缀合，以及ii）特异性结合第二抗原上的第二结合位点，其与人源的第二Fc区多肽缀合，其中所述方法包括以下步骤：将表达第一抗原和第二抗原的细胞与组合物 ; 向混合物中加入兔子补体; 并确定细胞裂解，从而确定组合物的补体依赖性细胞毒性。

7. 发明申请

WO2017167714A1 B-CELL CULTIVATION METHOD 审中-公开
标题翻译： B细胞培养方法
公开(公告)号：WO2017167714A1
公开(公告)日：2017-10-05
申请号：PCT/EP2017/057250
申请日：2017-03-28
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： OFFNER, Sonja , JUNG, Friederike
IPC分类号： C12N5/0781
摘要： Herein is reported a method for co-cultivating one or more B-cells comprising the step of incubating the one or more B-cells with EL4-B5 cells, whereby the EL4-B5 cells have been obtained/are from a cultivation of EL4-B5 cells that has a cell density of from 600,000 cells/ml up to 1,500,000 cells/ml.
摘要翻译：这里报道了共培养一种或多种B细胞的方法，包括将一种或多种B细胞与EL4-B5细胞一起温育的步骤，由此获得EL4-B5细胞 /来自细胞密度为600,000个细胞/ ml至1,500,000个细胞/ ml的EL4-B5细胞的培养。

8. 发明申请

WO2019105864A1 B-CELL CULTIVATION METHOD 审中-公开
公开(公告)号：WO2019105864A1
公开(公告)日：2019-06-06
申请号：PCT/EP2018/082401
申请日：2018-11-23
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： OFFNER, Sonja , JUNG, Friederike
IPC分类号： C12N5/0781 , C07K16/00
CPC分类号： C12N5/0635 , C07K16/00 , C12N2501/2301 , C12N2501/2302 , C12N2501/2304 , C12N2501/2306 , C12N2501/231 , C12N2501/2321 , C12N2501/25 , C12N2502/1114 , C12N2502/1157 , C12N2502/1185 , C12N2529/00
摘要： Herein is reported a method for the co-cultivation of single deposited B-cells, which can be of any source, with EL-4 B5 feeder cells in a suitable co-cultivation medium. In the herein reported methods the EL-4 B5 cells have been irradiated with a dose of less than 40 Gy, preferably 9.5 Gy or less. Thereby the EL-4 B5 cells have a higher viability and maintain the ability to divide in cultivation at doses less than 6 Gy.

9. 发明申请

WO2020141145A1 ANTI-RABBIT CD19 ANTIBODIES AND METHODS OF USE 审中-公开
公开(公告)号：WO2020141145A1
公开(公告)日：2020-07-09
申请号：PCT/EP2019/087064
申请日：2019-12-27
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： HAAS, Alexander , JUNG, Friederike , KLOSTERMANN, Stefan , OFFNER, Sonja
IPC分类号： C07K16/28
摘要： Herein is reported an antibody binding to rabbit CD19 comprising (a) a HVR-H1 comprising the amino acid sequence of SEQ ID NO: 32 or 33 or 34, (b) a HVR-H2 comprising the amino acid sequence of SEQ ID NO: 35 or 36, (c) a HVR-H3 comprising the amino acid sequence of SEQ ID NO: 37, (d) a HVR-L1 comprising the amino acid sequence of SEQ ID NO: 38, (e) a HVR-L2 comprising the amino acid sequence of SEQ ID NO: 39, and (f) a HVR-L3 comprising the amino acid sequence of SEQ ID NO: 40, as well as methods of using the same, especially in the identification and selection of antibody producing rabbit B-cells.

10. 发明申请

WO2017216098A1 ASSAY AND METHOD FOR DETERMINING CDC ELICITING ANTIBODIES 审中-公开
标题翻译：测定CDC抗体的测定和方法
公开(公告)号：WO2017216098A1
公开(公告)日：2017-12-21
申请号：PCT/EP2017/064272
申请日：2017-06-12
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： DENGL, Stefan , OFFNER, Sonja
IPC分类号： G01N33/50
摘要： Herein is reported a method for determining complement dependent cytotoxicity of a composition comprising i) a first binding site that specifically binds to a first epitope on a first antigen, which is conjugated to a first Fc-region polypeptide of human origin, and ii) a second binding site that specifically binds to a second epitope on a second antigen, which is conjugated to a second Fc-region polypeptide of human origin, wherein the method comprises the steps of incubating a cell expressing the first antigen and the second antigen with the composition and a mixture of anti-m CRP antibodies; adding normal human serum or rabbit complement to the mixture; and determining cell lysis and thereby determining complement dependent cytotoxicity of the composition.
摘要翻译：本文报道了用于确定组合物的补体依赖性细胞毒性的方法，所述组合物包含：i）特异性结合第一抗原上的第一表位的第一结合位点，其与第一Fc-区多肽和ii）与第二抗原上的第二表位特异性结合的第二结合位点，所述第二抗原与人起源的第二Fc区多肽缀合，其中所述方法包括以下步骤：将表达第一抗原和具有该组合物的第二抗原以及抗m CRP抗体的混合物; 向混合物中加入正常人血清或兔补体; 并确定细胞溶解并由此确定组合物的补体依赖性细胞毒性。

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