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1. 发明授权

US5264366A Protease deficient bacillus 失效
标题翻译：蛋白酶缺陷型杆菌
公开(公告)号：US5264366A
公开(公告)日：1993-11-23
申请号：US797577
申请日：1991-11-25
申请人： Eugenio Ferrari , Dennis J. Henner , Mark L. Stahl
发明人： Eugenio Ferrari , Dennis J. Henner , Mark L. Stahl
IPC分类号： C11D3/386 , C12N9/54 , C12N15/10 , C12N15/75 , C12N15/00 , C12N15/03 , C12N15/31
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： There are described, normally sporulating mutant Bacillus strain(s) which produce no detectable proteolytic activity during any phase of its growth. The absence of detectable proteolytic activity is due to the deletion of one or more codons specifying the mature subtilisin protease and the mature neutral protease. Also described are methods for producing such normally sporulating, protease deficient Bacillus mutants.
摘要翻译：描述了通常是孢子形成的突变芽孢杆菌菌株，其在其生长的任何阶段都不产生可检测的蛋白水解活性。不存在可检测的蛋白水解活性是由于缺失一个或多个密码子，指定成熟的枯草杆菌蛋白酶和成熟的中性蛋白酶。还描述了用于产生这种通常形成孢子的蛋白酶缺陷芽孢杆菌突变体的方法。

2. 发明授权

US06801860B1 Crystal structure of cPLA2 and methods of identifying agonists and antagonists using same 失效
标题翻译： cPLA2的晶体结构和使用其的激动剂和拮抗剂的鉴定方法
公开(公告)号：US06801860B1
公开(公告)日：2004-10-05
申请号：US09250083
申请日：1999-02-15
申请人： Andrea Dessen , William S. Somers , Mark L. Stahl , Jasbir S. Seehra
发明人： Andrea Dessen , William S. Somers , Mark L. Stahl , Jasbir S. Seehra
IPC分类号： G06F9455
CPC分类号： C12N9/20
摘要： The present invention provides for crystalline cPLA2. The crystal structure of cPLA2 has also been solved using such material. Models based upon such crystal structure are also provided. Methods of identifying inhibitors of cPLA2 activity and membrane binding using such models are also disclosed.
摘要翻译：本发明提供了结晶cPLA2。 cPLA2的晶体结构也使用这种材料来解决。还提供了基于这种晶体结构的模型。还公开了使用这些模型鉴定cPLA2活性抑制剂和膜结合的方法。

3. 发明授权

US07155346B2 Crystal structure of yqeJ and uses thereof 失效
公开(公告)号：US07155346B2
公开(公告)日：2006-12-26
申请号：US10209041
申请日：2002-07-31
申请人： Andrea M. Olland , Mark L. Stahl , Francis X. Sullivan , Kathryn W. Underwood , Rajiv Chopra
发明人： Andrea M. Olland , Mark L. Stahl , Francis X. Sullivan , Kathryn W. Underwood , Rajiv Chopra
IPC分类号： G01N33/48 , C12Q1/48
CPC分类号： G01N25/147 , G06F19/70
摘要： This invention is directed to the crystal structure of yqeJ, and to the use of this structure in rational drug design methods to identify agents that may interact with active sites of yqeJ. Such agents may be useful as antibacterial agents.

4. 发明授权

US07167802B2 Methods for identifying agents that interact with MAP kinase activated protein kinase 2 失效
标题翻译：识别与MAP激酶活化蛋白激酶2相互作用的试剂的方法
公开(公告)号：US07167802B2
公开(公告)日：2007-01-23
申请号：US10294027
申请日：2002-11-13
申请人： Kevin D. Parris , Kathryn W. Underwood , Mark L. Stahl , Lidia Mosyak , Kristine Svenson , Tania Shane , Meggin L. Taylor
发明人： Kevin D. Parris , Kathryn W. Underwood , Mark L. Stahl , Lidia Mosyak , Kristine Svenson , Tania Shane , Meggin L. Taylor
IPC分类号： G06F19/00
CPC分类号： C12N9/1205 , C07H21/04 , C07K2299/00 , Y02A90/26
摘要： This invention is directed to the crystal structures of MK2, and to the use of these structures in rational drug design methods to identify agents that may interact with active sites of MK2 proteins. Such agents may be useful as therapeutic agents.
摘要翻译：本发明涉及MK2的晶体结构，以及在合理的药物设计方法中使用这些结构来鉴定可能与MK2蛋白的活性位点相互作用的试剂。这样的试剂可用作治疗剂。

5. 发明授权

US06461604B1 Crystalline IL-6 and models of the molecular structure of IL-6 失效
标题翻译：结晶IL-6和IL-6分子结构模型
公开(公告)号：US06461604B1
公开(公告)日：2002-10-08
申请号：US09499148
申请日：2000-02-07
申请人： William S. Somers , Mark L. Stahl , Jasbir S. Seehra , Guang-Yi Xu , Thomas E. McDonagh , Hsiang-Ai Yu , Jin Hong
发明人： William S. Somers , Mark L. Stahl , Jasbir S. Seehra , Guang-Yi Xu , Thomas E. McDonagh , Hsiang-Ai Yu , Jin Hong
IPC分类号： C07K130
CPC分类号： C07K14/5412
摘要： Crystallographic and NMR solution structures of human IL-6 are reported. The invention provides models and systems incorporating such structures which are useful for identifying IL-6/IL-6 receptor interactions and for identification of agonists and antagonists of such interactions. Crystalline human IL-6 is also provided.
摘要翻译：报道了人IL-6的晶体和NMR溶液结构。本发明提供了结合这样的结构的模型和系统，其可用于鉴定IL-6 / IL-6受体相互作用并用于鉴定此类相互作用的激动剂和拮抗剂。还提供了结晶人IL-6。

6. 发明授权

US06459996B1 Crystal structure of E. coli GDP-fucose synthetase (and complexes thereof) and methods of identifying agonists and antagonists using same 失效
标题翻译：大肠杆菌GDP-岩藻糖合成酶（及其复合物）的晶体结构以及使用它们鉴定激动剂和拮抗剂的方法
公开(公告)号：US06459996B1
公开(公告)日：2002-10-01
申请号：US09373432
申请日：1999-08-13
申请人： William S. Somers , Mark L. Stahl , Francis X. Sullivan
发明人： William S. Somers , Mark L. Stahl , Francis X. Sullivan
IPC分类号： G01N3100
CPC分类号： C12N9/0006 , C12N9/90
摘要： The present invention provides for crystalline GFS. The crystal structure of GFS has also been solved using such material. Models based upon such crystal structure are also provided. Methods of identifying inhibitors of GFS activity using such models are also disclosed.
摘要翻译：本发明提供了结晶GFS。 GFS的晶体结构也使用这种材料得到解决。还提供了基于这种晶体结构的模型。还公开了使用这些模型鉴定GFS活性抑制剂的方法。

7. 发明授权

US4801536A Method for producing heterologous proteins 失效
标题翻译：异源蛋白质的制备方法
公开(公告)号：US4801536A
公开(公告)日：1989-01-31
申请号：US57881
申请日：1987-06-02
申请人： Mark L. Stahl , Edward R. LaVallie
发明人： Mark L. Stahl , Edward R. LaVallie
IPC分类号： C07K14/32 , C07K14/62 , C12N15/62 , C12N15/90 , C12P21/00 , C12N15/00
CPC分类号： C12N15/62 , C07K14/32 , C07K14/62 , C12N15/90 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C07K2319/50 , C07K2319/75
摘要： This invention concerns a method for producing a heterologous protein in a bacterial host cell such that the protein is exported from the host cell into the culture medium. The method involves culturing in a bacterial culture medium a genetically engineered bacterial strain containing a fusion DNA sequence comprising a first nucleotide sequence encoding at least an N-terminal portion of a flagellin protein and a second nucleotide sequence encoding the heterologous protein. The first nucleotide sequence is linked via its 3' terminus to the 5' terminus of the second nucleotide sequence, and the fusion DNA sequence is itself linked to an expression control sequence. In certain embodiments the first and second nucleotide sequences are linked by means of a linking nucleotide sequence encoding a selectively cleavable polypeptide, In those embodiments the resulting exported fusion protein will contain a selectively cleavable site at which the fusion protein may be selectively cleaved by chemical or enzymatic methods to produce the heterologous protein encoded for by the second nucleotide sequence of the fusion DNA sequence. The heterologous protein may then be separately recovered from any polypeptide fragment of flagellin or other proteinaceous material.
摘要翻译：本发明涉及在细菌宿主细胞中产生异源蛋白质的方法，使得蛋白质从宿主细胞输出到培养基中。所述方法包括在细菌培养基中培养含有融合DNA序列的遗传工程改造的细菌菌株，所述融合DNA序列包含编码鞭毛蛋白蛋白质的至少N末端部分的第一核苷酸序列和编码异源蛋白质的第二核苷酸序列。第一个核苷酸序列通过其3'末端连接到第二个核苷酸序列的5'末端，并且融合DNA序列本身与表达控制序列连接。在某些实施方案中，第一和第二核苷酸序列通过编码可选择性切割多肽的连接核苷酸序列连接。在这些实施方案中，所得出口的融合蛋白将含有可选择性切割位点，在该位点处，融合蛋白可以被化学或用于产生由融合DNA序列的第二个核苷酸序列编码的异源蛋白质的酶学方法。然后可以从鞭毛蛋白或其它蛋白质材料的任何多肽片段单独回收异源蛋白质。

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