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1. 发明申请

US20050176592A1 Method of using intrinsically conductive polymers with inherent lubricating properties, and a composition having an intrinsically conductive polymer, for protecting metal surfaces from galling and corrosion 审中-公开
标题翻译：使用具有固有润滑性能的固有导电聚合物的方法，以及具有固有导电聚合物的组合物，用于保护金属表面免受磨损和腐蚀
公开(公告)号：US20050176592A1
公开(公告)日：2005-08-11
申请号：US10775086
申请日：2004-02-11
申请人： Ernesto Calvo , Mariano Bossi , Leandro Bronstein , Cesar Barbero , Liliana Trevani , Teresa Perez , Pablo Castro
发明人： Ernesto Calvo , Mariano Bossi , Leandro Bronstein , Cesar Barbero , Liliana Trevani , Teresa Perez , Pablo Castro
IPC分类号： B05D7/14 , C08L79/02 , C09D5/24 , C09D5/44 , C10M103/02 , C10M103/06 , C10M111/02 , C10M119/24 , C10M149/04 , C10M169/04 , C23C22/83 , C23F11/173 , C25D13/08 , F16B33/00 , F16L15/00 , F16L15/02 , F16L57/06 , F16L58/04 , F16L58/10 , C10M17/40 , C10M17/44
CPC分类号： F16L57/06 , C08L79/02 , C09D5/24 , C09D5/4419 , C09D5/4476 , C10M169/04 , C10M169/041 , C10M2201/041 , C10M2201/0413 , C10M2201/065 , C10M2201/0653 , C10M2215/223 , C10M2217/046 , C10N2230/00 , C10N2230/12 , C10N2250/08 , C10N2250/121 , C10N2260/10 , C23C22/83 , C25D13/08 , F16L15/08 , F16L58/10 , F16L58/182
摘要： A method for protecting a metal surface from galling and corrosion includes a step of providing a protective dry film on the metal surface. The film includes a solid lubricant and a conducting polymer, the conducting polymer having lubricant properties and being capable of binding the solid lubricant to the metal surface. Threaded metal joint surfaces coated with the film are capable of resisting galling under high pressure and high torque conditions, even after several fastening and unfastening operations or over a long period of time. Protection from corrosion is also provided by the film. The method and film are economical in that only a single layer of protective compound need be applied in order to provide metal surfaces with both lubrication and protection against corrosion, and problems such as removal or leakage, which are associated with protective compounds that use oils, are avoided. Additionally, the dry film is advantageous because it does not contain heavy metals that are harmful to the environment.
摘要翻译：用于保护金属表面免受磨损和腐蚀的方法包括在金属表面上提供保护性干膜的步骤。该膜包括固体润滑剂和导电聚合物，导电聚合物具有润滑性能并能够将固体润滑剂与金属表面结合。涂覆有薄膜的螺纹金属接头表面即使在几次紧固和松开操作之后或长时间内也能够在高压和高扭矩条件下抵抗磨损。薄膜还提供防腐蚀。该方法和薄膜是经济的，因为仅需要施加一层保护化合物，以便为金属表面提供润滑和防腐蚀，以及与使用油的保护性化合物相关的诸如去除或渗漏的问题，被避免。此外，干膜是有利的，因为它不含有对环境有害的重金属。

2. 发明申请

US20120135459A1 NOVEL FLUORINATED RHODAMINES AS PHOTOSTABLE FLUORESCENT DYES FOR LABELLING AND IMAGING TECHNIQUES 有权
标题翻译：新型荧光罗丹明作为标示和成像技术的可光化荧光染料
公开(公告)号：US20120135459A1
公开(公告)日：2012-05-31
申请号：US13380148
申请日：2009-06-26
申请人： Stefan W. Hell , Vladimir N. Belov , Gyuzel Mitronova , Mariano Bossi , Gael Moneron , Christian A. Wurm , Stefan Jakobs , Christian Eggeling , Jakob Bierwagen , Lars Meyer
发明人： Stefan W. Hell , Vladimir N. Belov , Gyuzel Mitronova , Mariano Bossi , Gael Moneron , Christian A. Wurm , Stefan Jakobs , Christian Eggeling , Jakob Bierwagen , Lars Meyer
IPC分类号： G01N21/64 , C07K17/02 , C07H21/04 , C07D493/10
CPC分类号： C07D311/82 , A61K49/0041 , A61K49/0058 , C07K1/13 , C09B11/24 , G01N33/582
摘要： The present invention relates to novel fluorinated 3,6-diaminoxanthene compounds derived from the basic structural formula (I) and to their uses as photostable fluorescent dyes, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional microscopy, stimulated emission depletion (STED) reversible saturable optically linear fluorescent transitions (RESOLFT) microscopy, and fluorescence correlation spectroscopy. The claimed compounds are also useful as molecular probes in various spectroscopic applications.
摘要翻译：本发明涉及衍生自基本结构式（I）的新型氟化3,6-二氨基呫吨化合物及其作为光稳定荧光染料的用途。用于免疫染色和光谱和微观应用，特别是在常规显微镜，受激发射耗尽（STED）可逆饱和光学线性荧光转换（RESOLFT）显微镜和荧光相关光谱学中。所要求保护的化合物也可用作各种光谱应用中的分子探针。

3. 发明授权

US08735444B2 Fluorinated rhodamines as photostable fluorescent dyes for labelling and imaging techniques 有权
标题翻译：氟化罗丹明作为用于标记和成像技术的光稳定荧光染料
公开(公告)号：US08735444B2
公开(公告)日：2014-05-27
申请号：US13380148
申请日：2009-06-26
申请人： Stefan W. Hell , Vladimir N. Belov , Gyuzel Mitronova , Mariano Bossi , Gael Moneron , Christian A. Wurm , Stefan Jakobs , Christian Eggeling , Jakob Bierwagen , Lars Meyer
发明人： Stefan W. Hell , Vladimir N. Belov , Gyuzel Mitronova , Mariano Bossi , Gael Moneron , Christian A. Wurm , Stefan Jakobs , Christian Eggeling , Jakob Bierwagen , Lars Meyer
IPC分类号： A61K31/352 , C07D311/82
CPC分类号： C07D311/82 , A61K49/0041 , A61K49/0058 , C07K1/13 , C09B11/24 , G01N33/582
摘要： The present invention relates to novel fluorinated 3,6-diaminoxanthene compounds derived from the basic structural formula (I) and to their uses as photostable fluorescent dyes, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional microscopy, stimulated emission depletion (STED) reversible saturable optically linear fluorescent transitions (RESOLFT) microscopy, and fluorescence correlation spectroscopy. The claimed compounds are also useful as molecular probes in various spectroscopic applications.
摘要翻译：本发明涉及衍生自基本结构式（I）的新型氟化3,6-二氨基呫吨化合物及其作为光稳定荧光染料的用途。用于免疫染色和光谱和微观应用，特别是在常规显微镜，受激发射耗尽（STED）可逆饱和光学线性荧光转换（RESOLFT）显微镜和荧光相关光谱学中。所要求保护的化合物也可用作各种光谱应用中的分子探针。

4. 发明授权

US07880150B2 High spatial resolution imaging of a structure of interest in a specimen 有权
标题翻译：样本中感兴趣结构的高空间分辨率成像
公开(公告)号：US07880150B2
公开(公告)日：2011-02-01
申请号：US12128948
申请日：2008-05-29
申请人： Stefan Hell , Christian Eggeling , Alexander Egner , Jonas Fölling , Andreas Schönle , Mariano Bossi
发明人： Stefan Hell , Christian Eggeling , Alexander Egner , Jonas Fölling , Andreas Schönle , Mariano Bossi
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01N21/6428 , G02B21/16 , G02B21/367 , G02B27/58
摘要： For the high spatial resolution imaging of a structure of interest in a specimen, a substance is selected from a group of substances which have a fluorescent first state and a nonfluorescent second state; which can be converted fractionally from their first state into their second state by light which excites them into fluorescence, and which return from their second state into their first state; the specimen's structure of interest is imaged onto a sensor array, a spatial resolution limit of the imaging being greater (i.e. worse) than an average spacing between closest neighboring molecules of the substance in the specimen; the specimen is exposed to light in a region which has dimensions larger than the spatial resolution limit, fractions of the substance alternately being excited by the light to emit fluorescent light and converted into their second state, and at least 10% of the molecules of the substance that are respectively in the first state lying at a distance from their closest neighboring molecules in the first state which is greater than the spatial resolution limit; and the fluorescent light, which is spontaneously emitted by the substance from the region, is registered in a plurality of images recorded by the sensor array during continued exposure of the specimen to the light.
摘要翻译：对于样本中感兴趣的结构的高空间分辨率成像，物质选自具有荧光第一状态和非荧光第二状态的物质组; 其可以通过将它们激发成荧光的光从其第一状态分数转换成第二状态，并且从它们的第二状态返回到其第一状态; 将感兴趣的标本的结构成像到传感器阵列上，成像的空间分辨率极限比样品中物质的最接近的相邻分子之间的平均间距更大（即更差）试样在具有大于空间分辨率极限的尺寸的区域中暴露于光，该物质的部分交替地被光激发以发射荧光并转换成其第二状态，并且至少10％的分别处于第一状态的物质，其处于与第一状态相比最大相邻分子一定距离，该距离大于空间分辨率极限; 并且由该区域内的物质自发地发出的荧光被记录在传感器阵列记录的多个图像中，该样本在样品持续暴露于光下。

5. 发明申请

US20110160083A1 HIGH SPATIAL RESOLUTION IMAGING OF A STRUCTURE OF INTEREST IN A SPECIMEN 有权
标题翻译：一个样本中利益结构的高空间分辨率成像
公开(公告)号：US20110160083A1
公开(公告)日：2011-06-30
申请号：US13013359
申请日：2011-01-25
申请人： Stefan Hell , Christian Eggeling , Alexander Egner , Jonas Fölling , Andreas Schönle , Mariano Bossi
发明人： Stefan Hell , Christian Eggeling , Alexander Egner , Jonas Fölling , Andreas Schönle , Mariano Bossi
IPC分类号： C40B30/04 , C40B30/10
CPC分类号： G01N21/6458 , G01N21/6428 , G02B21/16 , G02B21/367 , G02B27/58
摘要： For imaging of a structure, the structure is marked with a substance which can be converted by a switching signal from a first into a second state, and which provides an optical measurement signal in one of its states, only. The switching signal is applied such that at least 10% of the molecules of the substance being in the measurement signal providing state are at a distance from their closest neighbors, which is greater than the spatial resolution limit of imaging the specimen onto a sensor array, which in turn is greater than an average distance between the molecules of the substance. From an intensity distribution of the measurement signal recorded with the sensor array, the position is only determined for those molecules of the substance which are at a distance from their closest neighboring molecules in the measurement signal providing state, which is greater than the spatial resolution limit.
摘要翻译：对于结构的成像，结构用可以通过开关信号从第一状态转换为第二状态并且仅在其状态之一提供光学测量信号的物质标记。施加开关信号，使得处于测量信号提供状态的物质的至少10％的分子距离它们最近的邻近物一个距离，其大于将样本成像到传感器阵列上的空间分辨率极限，其又大于物质分子之间的平均距离。根据记录有传感器阵列的测量信号的强度分布，该位置仅对于在测量信号提供状态中离它们最近的相邻分子一定距离的物质的那些分子确定，其大于空间分辨率极限。

6. 发明授权

US08174692B2 High spatial resolution imaging of a structure of interest in a specimen 有权
标题翻译：样本中感兴趣结构的高空间分辨率成像
公开(公告)号：US08174692B2
公开(公告)日：2012-05-08
申请号：US12949159
申请日：2010-11-18
申请人： Stefan W. Hell , Jonas Fölling , Christian Eggeling , Alexander Egner , Andreas Schönle , Mariano Bossi
发明人： Stefan W. Hell , Jonas Fölling , Christian Eggeling , Alexander Egner , Andreas Schönle , Mariano Bossi
IPC分类号： G01J3/30
CPC分类号： G01N21/6458 , G01N21/6428 , G02B21/16 , G02B21/367 , G02B27/58
摘要： In high spatial resolution imaging, a structure in a specimen is marked with a substance which, in a first electronic state, is excited by light of one wavelength to emit fluorescent light, which is also converted from its first into a second electronic state by that light, and which returns from its second into its first electronic state. The specimen is imaged onto a sensor at a spatial resolution not resolving an average spacing between neighboring molecules of the substance, and exposed to the light at such an intensity that the molecules in the first state are alternately excited to emit fluorescent light and converted into their second state, and that at least 10% of the molecules presently in their first state lie at a distance from their closest neighboring molecules in their first state which is greater than the spatial resolution of the imaging onto the sensor.
摘要翻译：在高空间分辨率成像中，样本中的结构用物质标记，该物质在第一电子状态下被一个波长的光激发以发射荧光，其也从其第一个电子状态转换成第二电子状态光，从第二个回到第一个电子状态。将样本以不分辨物质的相邻分子之间的平均间隔的空间分辨率成像到传感器上，并以如此强度暴露于光，使得第一状态下的分子被交替地激发以发射荧光并转换成它们并且目前处于其第一状态的至少10％的分子处于与它们的第一状态中最接近的相邻分子相距一定距离，其大于成像在传感器上的空间分辨率。

7. 发明授权

US08084754B2 High spatial resolution imaging of a structure of interest in a specimen 有权
标题翻译：样本中感兴趣结构的高空间分辨率成像
公开(公告)号：US08084754B2
公开(公告)日：2011-12-27
申请号：US13013359
申请日：2011-01-25
申请人： Stefan Hell , Christian Eggeling , Alexander Egner , Jonas Fölling , Andreas Schönle , Mariano Bossi
发明人： Stefan Hell , Christian Eggeling , Alexander Egner , Jonas Fölling , Andreas Schönle , Mariano Bossi
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01N21/6428 , G02B21/16 , G02B21/367 , G02B27/58
摘要： For imaging of a structure, the structure is marked with a substance which can be converted by a switching signal from a first into a second state, and which provides an optical measurement signal in one of its states, only. The switching signal is applied such that at least 10% of the molecules of the substance being in the measurement signal providing state are at a distance from their closest neighbors, which is greater than the spatial resolution limit of imaging the specimen onto a sensor array, which in turn is greater than an average distance between the molecules of the substance. From an intensity distribution of the measurement signal recorded with the sensor array, the position is only determined for those molecules of the substance which are at a distance from their closest neighboring molecules in the measurement signal providing state, which is greater than the spatial resolution limit.
摘要翻译：对于结构的成像，结构用可以通过开关信号从第一状态转换为第二状态并且仅在其状态之一提供光学测量信号的物质标记。施加开关信号，使得处于测量信号提供状态的物质的至少10％的分子距离它们最近的邻近物一个距离，其大于将样本成像到传感器阵列上的空间分辨率极限，其又大于物质分子之间的平均距离。根据记录有传感器阵列的测量信号的强度分布，该位置仅对于在测量信号提供状态中离它们最近的相邻分子一定距离的物质的那些分子确定，其大于空间分辨率极限。

8. 发明申请

US20090134342A1 High spatial resolution imaging of a structure of interest in a specimen 有权
标题翻译：样本中感兴趣结构的高空间分辨率成像
公开(公告)号：US20090134342A1
公开(公告)日：2009-05-28
申请号：US12128948
申请日：2008-05-29
申请人： Stefan Hell , Christian Eggeling , Alexander Egner , Jonas Folling , Andreas Schonle , Mariano Bossi
发明人： Stefan Hell , Christian Eggeling , Alexander Egner , Jonas Folling , Andreas Schonle , Mariano Bossi
IPC分类号： G01J1/58 , G01N1/30
CPC分类号： G01N21/6458 , G01N21/6428 , G02B21/16 , G02B21/367 , G02B27/58
摘要： For the high spatial resolution imaging of a structure of interest in a specimen, a substance is selected from a group of substances which have a fluorescent first state and a nonfluorescent second state; which can be converted fractionally from their first state into their second state by light which excites them into fluorescence, and which return from their second state into their first state; the specimen's structure of interest is imaged onto a sensor array, a spatial resolution limit of the imaging being greater (i.e. worse) than an average spacing between closest neighboring molecules of the substance in the specimen; the specimen is exposed to light in a region which has dimensions larger than the spatial resolution limit, fractions of the substance alternately being excited by the light to emit fluorescent light and converted into their second state, and at least 10% of the molecules of the substance that are respectively in the first state lying at a distance from their closest neighboring molecules in the first state which is greater than the spatial resolution limit; and the fluorescent light, which is spontaneously emitted by the substance from the region, is registered in a plurality of images recorded by the sensor array during continued exposure of the specimen to the light.
摘要翻译：对于样本中感兴趣的结构的高空间分辨率成像，物质选自具有荧光第一状态和非荧光第二状态的物质组; 其可以通过将它们激发成荧光的光从其第一状态分数转换成第二状态，并且从它们的第二状态返回到其第一状态; 将感兴趣的标本的结构成像到传感器阵列上，成像的空间分辨率极限比样品中物质的最接近的相邻分子之间的平均间距更大（即更差）试样在具有大于空间分辨率极限的尺寸的区域中暴露于光，该物质的部分交替地被光激发以发射荧光并转换成其第二状态，并且至少10％的分别处于第一状态的物质，其处于与第一状态相比最大相邻分子一定距离，该距离大于空间分辨率极限; 并且由该区域内的物质自发地发出的荧光被记录在传感器阵列记录的多个图像中，该样本在样品持续暴露于光下。

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