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1. 发明授权

US09163329B2 RNA labeling method 有权
标题翻译： RNA标记方法
公开(公告)号：US09163329B2
公开(公告)日：2015-10-20
申请号：US13354023
申请日：2012-01-19
申请人： Emily Marine Leproust , Gusti Zeiner , Petula N. D'andrade
发明人： Emily Marine Leproust , Gusti Zeiner , Petula N. D'andrade
IPC分类号： C12Q1/68 , C40B20/02 , C40B20/04 , C40B70/00
CPC分类号： C40B20/02 , C12Q1/6806 , C12Q1/6837 , C12Q1/6855 , C40B20/04 , C40B70/00 , C12Q1/6809 , C12Q2525/191 , C12Q2525/207 , C12Q2565/501
摘要： A method of sample analysis is provided. In certain embodiments, the method involves: a) obtaining a fragmented RNA sample comprising fragments of long RNA molecules and short RNA molecules; b) ligating an adaptor to an end of the RNA of the fragmented RNA sample to produce an adaptor-ligated sample; c) hybridizing said adaptor-ligated sample to an array of nucleic acid probes; and d) reading said array to obtain an estimate of the abundance of a long RNA in the RNA sample and an estimate of the abundance a small RNA in the RNA sample.
摘要翻译：提供了样品分析的方法。在某些实施方案中，该方法包括：a）获得包含长RNA分子和短RNA分子片段的片段化RNA样品; b）将适配器连接到片段化RNA样品的RNA的末端以产生衔接子连接的样品; c）将所述衔接子连接的样品与核酸探针阵列杂交; 和d）读取所述阵列以获得RNA样品中长RNA的丰度的估计，以及RNA样品中的小RNA的丰度估计。

2. 发明申请

US20120122702A1 RNA LABELING METHOD 有权
标题翻译： RNA标记方法
公开(公告)号：US20120122702A1
公开(公告)日：2012-05-17
申请号：US13354023
申请日：2012-01-19
申请人： Emily Marine Leproust , Gusti Zeiner , Petula N. D'andrade
发明人： Emily Marine Leproust , Gusti Zeiner , Petula N. D'andrade
IPC分类号： C40B30/04 , C40B40/06 , C40B20/00
CPC分类号： C40B20/02 , C12Q1/6806 , C12Q1/6837 , C12Q1/6855 , C40B20/04 , C40B70/00 , C12Q1/6809 , C12Q2525/191 , C12Q2525/207 , C12Q2565/501
摘要： A method of sample analysis is provided. In certain embodiments, the method involves: a) obtaining a fragmented RNA sample comprising fragments of long RNA molecules and short RNA molecules; b) ligating an adaptor to an end of the RNA of the fragmented RNA sample to produce an adaptor-ligated sample; c) hybridizing said adaptor-ligated sample to an array of nucleic acid probes; and d) reading said array to obtain an estimate of the abundance of a long RNA in the RNA sample and an estimate of the abundance a small RNA in the RNA sample.
摘要翻译：提供了样品分析的方法。在某些实施方案中，该方法包括：a）获得包含长RNA分子和短RNA分子片段的片段化RNA样品; b）将适配器连接到片段化RNA样品的RNA的末端以产生衔接子连接的样品; c）将所述衔接子连接的样品与核酸探针阵列杂交; 和d）读取所述阵列以获得RNA样品中长RNA的丰度的估计，以及RNA样品中的小RNA的丰度估计。

3. 发明授权

US09074203B2 Ligation method employing RtcB 有权
标题翻译：使用RtcB的连接方法
公开(公告)号：US09074203B2
公开(公告)日：2015-07-07
申请号：US13368600
申请日：2012-02-08
申请人： Gusti Zeiner , Robert A. Ach
发明人： Gusti Zeiner , Robert A. Ach
IPC分类号： C12P21/04 , C12N15/10 , C12N15/66 , C12N9/00 , C12P19/34
CPC分类号： C12N15/10 , C12N9/93 , C12N15/66 , C12P19/34 , C12Y605/01004
摘要： A method of processing an RNA sample is provided. In certain embodiments, the method may comprise: a) obtaining a fragmented RNA sample comprising: i. RNA fragments of long RNA molecules; and ii. unfragmented short RNA; and b) contacting said fragmented RNA sample with a first adaptor in the presence of a RtcB ligase, thereby producing a ligated RNA sample comprising adaptor-ligated fragments of long RNA. A kit for performing the method is also provided.
摘要翻译：提供了一种处理RNA样品的方法。在某些实施方案中，该方法可以包括：a）获得片段化的RNA样品，其包含：长RNA分子的RNA片段; 和ii。未分片的短RNA; 和b）在RtcB连接酶存在下使所述片段化RNA样品与第一衔接子接触，从而产生连接的RNA样品，其包含长连接的连接的长RNA片段。还提供了用于执行该方法的套件。

4. 发明授权

US08927245B2 Ligation method employing eukaryotic tRNA ligase 有权
标题翻译：使用真核tRNA连接酶的连接方法
公开(公告)号：US08927245B2
公开(公告)日：2015-01-06
申请号：US13327572
申请日：2011-12-15
申请人： Gusti Zeiner , Robert A. Ach
发明人： Gusti Zeiner , Robert A. Ach
IPC分类号： C12N9/00 , C07H21/02
CPC分类号： C12P19/34 , C12N15/10 , C12N15/1096 , C12Q2521/501 , C12Q2525/191
摘要： Provided herein is a method of preparing an RNA sample comprising: a) obtaining an RNA sample comprising: i. long RNA molecules that may be unfragmented or fragmented to contain 5′-OH group and a 2′-3′-cyclic phosphate group; and ii. short RNA molecules that comprise a 5′ phosphate group and a 3′ OH group; and b) contacting the RNA sample with an adaptor comprising either a 2′-PO group and 3′-OH group or a 2′,3′-cyclic phosphate group in the presence of a eukaryotic tRNA ligase, thereby producing a ligated RNA sample in which a) the short RNA molecules are selectively ligated to the adaptor or b) the short RNA molecules and long RNA fragments are selectively ligated to the adaptor.
摘要翻译：本文提供了制备RNA样品的方法，其包括：a）获得RNA样品，其包含：i。可能未被分裂或片段化以含有5'-OH基团和2'- 3'-环磷酸酯基团的长RNA分子; 和ii。包含5'磷酸基团和3'OH基团的短RNA分子; 和b）在真核tRNA连接酶的存在下，将RNA样品与包含2'-PO基团和3'-OH基团或2'，3'-环状磷酸酯基团的衔接子接触，从而产生连接的RNA样品其中a）短RNA分子选择性地连接到衔接子上，或b）短RNA分子和长RNA片段选择性地连接到衔接子上。

5. 发明申请

US20120208707A1 LIGATION METHOD EMPLOYING RTCB 有权
标题翻译：使用RTCB的方法
公开(公告)号：US20120208707A1
公开(公告)日：2012-08-16
申请号：US13368600
申请日：2012-02-08
申请人： Gusti Zeiner , Robert A. Ach
发明人： Gusti Zeiner , Robert A. Ach
IPC分类号： C40B20/00 , C12Q1/68 , C12N9/16 , C12P19/34
CPC分类号： C12N15/10 , C12N9/93 , C12N15/66 , C12P19/34 , C12Y605/01004
摘要： A method of processing an RNA sample is provided. In certain embodiments, the method may comprise: a) obtaining a fragmented RNA sample comprising: i. RNA fragments of long RNA molecules; and ii. unfragmented short RNA; and b) contacting said fragmented RNA sample with a first adaptor in the presence of a RtcB ligase, thereby producing a ligated RNA sample comprising adaptor-ligated fragments of long RNA. A kit for performing the method is also provided.
摘要翻译：提供了一种处理RNA样品的方法。在某些实施方案中，该方法可以包括：a）获得片段化的RNA样品，其包含：长RNA分子的RNA片段; 和ii。未分片的短RNA; 和b）在RtcB连接酶存在下使所述片段化RNA样品与第一衔接子接触，从而产生连接的RNA样品，其包含长连接的连接的长RNA片段。还提供了用于执行该方法的套件。

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