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1. 发明授权

US09932603B2 P0 gene silencing constructs and use 有权
公开(公告)号：US09932603B2
公开(公告)日：2018-04-03
申请号：US14128962
申请日：2012-06-15
申请人： Elodie Klein , Véronique Graff , David Gilmer , Véronique Brault , Guy Weyens , Marc Lefebvre
发明人： Elodie Klein , Véronique Graff , David Gilmer , Véronique Brault , Guy Weyens , Marc Lefebvre
IPC分类号： C12N15/82 , C12N15/113 , A01H5/06
CPC分类号： C12N15/8283 , A01H5/06 , C12N15/113 , C12N15/1131 , C12N15/8218
摘要： The present invention is related to a method for conveying viral resistance or tolerance to one or more virus(es), in particular to beet mild yellowing virus (BMYV) and to beet necrotic yellow vein virus (BNYVV) or to BMYV alone in a plant, in particular in a sugar beet plant. Furthermore, the present invention relates to the virus resistant or -tolerant plant obtained according to this method, as well as to seeds and progeny derived therefrom.

2. 发明申请

US20140317779A1 P0 GENE SILENCING CONSTRUCTS AND USE 有权
标题翻译： P0基因沉默结构和用途
公开(公告)号：US20140317779A1
公开(公告)日：2014-10-23
申请号：US14128962
申请日：2012-06-15
申请人： Elodie Klein , Véronique Graff , David Gilmer , Véronique Brault , Guy Weyens , Marc Lefebvre
发明人： Elodie Klein , Véronique Graff , David Gilmer , Véronique Brault , Guy Weyens , Marc Lefebvre
IPC分类号： C12N15/82 , C12N15/113
CPC分类号： C12N15/8283 , A01H5/06 , C12N15/113 , C12N15/1131 , C12N15/8218
摘要： A recombinant BMYV P0 viral nucleotide sequence when transcribed in a cell is capable of forming a double stranded self-complementary RNA sequence.
摘要翻译：当在细胞中转录时，重组BMYV P0病毒核苷酸序列能够形成双链自身互补RNA序列。

3. 发明授权

US06297428B1 Method for inducing viral resistance into a plant 有权
标题翻译：诱导病毒抗性进入植物的方法
公开(公告)号：US06297428B1
公开(公告)日：2001-10-02
申请号：US09242216
申请日：1999-02-08
申请人： Hubert Guilley , Gerard Jonard , Ken Richards , Salah Bouzoubaa , Claudine Bleykasten-Grosshans , Guy Weyens , Marc Lefebvre
发明人： Hubert Guilley , Gerard Jonard , Ken Richards , Salah Bouzoubaa , Claudine Bleykasten-Grosshans , Guy Weyens , Marc Lefebvre
IPC分类号： C12N504
CPC分类号： C07K14/005 , C12N15/8283 , C12N2770/40022
摘要： The present invention concerns a method for inducing resistance to a virus comprising a TGB3 sequence with the proviso that it is not the potato virus X, into a plant cell or plant, comprising the following steps: preparing a nucleic acid construct comprising a nucleic acid sequence corresponding to at least 70% of the nucleic acid sequence of TGB3 of said virus or its corresponding cDNA, being operably linked to one or more regulatory sequence(s) active in a plant, transforming a plant cell with the nucleic acid construct, and possibly regenerating a transgenic plant from the transformed plant cell. The present invention is also related to the plant obtained.
摘要翻译：本发明涉及一种诱导对病毒的抗性的方法，其包含TGB3序列，条件是不是马铃薯病毒X，其包括以下步骤：制备包含核酸序列的核酸构建体对应于所述病毒或其相应cDNA的TGB3的核酸序列的至少70％，其与植物中活性的一种或多种调节序列可操作地连接，用核酸构建体转化植物细胞，并且可能从转化的植物细胞再生转基因植物。本发明还涉及获得的植物。

4. 发明授权

US07645579B2 T227-1 flanking sequence 失效
标题翻译： T227-1侧翼序列
公开(公告)号：US07645579B2
公开(公告)日：2010-01-12
申请号：US11750196
申请日：2007-05-17
申请人： Guy Weyens , Steve Barnes , Inge Rosquin
发明人： Guy Weyens , Steve Barnes , Inge Rosquin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , C12N15/8275
摘要： A herbicide resistant transformed sugar beet that is detectable by the specific primers developed to match the DNA sequences that flank the left and/or right border region of the inserted transgenic DNA and the method of identifying primer pairs containing plant genomic DNA/plasmid DNA. More specifically, the present invention covers a specific glyphosate resistant sugar beet plant having an insertion of the transgenic material identified as the T227-1 event. The present invention additionally covers primer pairs: plant genomic DNA/Plasmid DNA that are herein identified. Additionally, these primer pairs for either the left or the right flanking regions make an event specific test for the T227-1 insert of transgenic material.
摘要翻译：一种抗除草剂转化的甜菜，其可通过与所插入的转基因DNA的左侧和/或右侧边界区域侧翼的DNA序列匹配的特异性引物以及鉴定含有植物基因组DNA /质粒DNA的引物对的方法来检测。更具体地说，本发明涵盖具有被鉴定为T227-1事件的转基因材料插入的具体的抗草甘膦耐糖甜菜植物。本发明另外涵盖引物对：本文鉴定的植物基因组DNA /质粒DNA。另外，对于左侧或右侧翼区域的这些引物对对转基因材料的T227-1插入物进行事件特异性检验。

5. 发明申请

US20090265817A1 T227-1 FLANKING SEQUENCE 失效
公开(公告)号：US20090265817A1
公开(公告)日：2009-10-22
申请号：US11750196
申请日：2007-05-17
申请人： Guy Weyens , Steve Barnes , Inge Rosquin
发明人： Guy Weyens , Steve Barnes , Inge Rosquin
IPC分类号： A01H5/00 , C12Q1/68
CPC分类号： C12Q1/6895 , C12N15/8275
摘要： A herbicide resistant transformed sugar beet that is detectable by the specific primers developed to match the DNA sequences that flank the left and/or right border region of the inserted transgenic DNA and the method of identifying primer pairs containing plant genomic DNA/plasmid DNA. More specifically, the present invention covers a specific glyphosate resistant sugar beet plant having an insertion of the transgenic material identified as the T227-1 event. The present invention additionally covers primer pairs: plant genomic DNA/Plasmid DNA that are herein identified. Additionally, these primer pairs for either the left or the right flanking regions make an event specific test for the T227-1 insert of transgenic material.
摘要翻译：一种抗除草剂转化的甜菜，其可通过与所插入的转基因DNA的左侧和/或右侧边界区域侧翼的DNA序列匹配的特异性引物以及鉴定含有植物基因组DNA /质粒DNA的引物对的方法来检测。更具体地说，本发明涵盖具有被鉴定为T227-1事件的转基因材料插入的具体的抗草甘膦耐糖甜菜植物。本发明另外涵盖引物对：本文鉴定的植物基因组DNA /质粒DNA。另外，对于左侧或右侧翼区域的这些引物对对转基因材料的T227-1插入物进行事件特异性检验。

6. 发明授权

US5969215A Method of plant tissue culture and regeneration 失效
标题翻译：植物组织培养和再生方法
公开(公告)号：US5969215A
公开(公告)日：1999-10-19
申请号：US615216
申请日：1996-03-14
申请人： Robert David Hall , Franciscus Andries Krens , Henricus Adrianus Verhoeven , Maria Colijn-Hooymans , James Martin Dunwell , Guy Weyens
发明人： Robert David Hall , Franciscus Andries Krens , Henricus Adrianus Verhoeven , Maria Colijn-Hooymans , James Martin Dunwell , Guy Weyens
IPC分类号： A01H4/00 , C12N5/10 , C12N15/09 , C12N15/00
CPC分类号： A01H4/005 , A01H4/008
摘要： Plants may be regenerated from stomatal cells or protoplasts of such cells. Prior to regeneration the cells or protoplasts may be genetically transformed by the introduction of hereditary material most preferably by a DNA construct which is free of genes which specify resistance to antibiotics. The regeneration step may include callus formation on a hormone-free medium. The method is particularly suitable for sugar beet.
摘要翻译： PCT No.PCT / GB94 / 02252 Sec。 371日期：1996年3月14日 102（e）1996年3月14日PCT 1994年10月14日PCT PCT。公开号WO95 / 10178 日期1995年04月20日可以从这种细胞的气孔细胞或原生质体再生植物。在再生之前，细胞或原生质体可以通过引入遗传物质进行遗传转化，最优选通过不含具有抗生素抗性的基因的DNA构建体。再生步骤可以包括在无激素培养基上形成愈伤组织。该方法特别适用于甜菜。

7. 发明授权

US07608754B2 Double fructan beets 有权
标题翻译：双果聚糖甜菜
公开(公告)号：US07608754B2
公开(公告)日：2009-10-27
申请号：US10415686
申请日：2002-06-25
申请人： Guy Weyens , Jean Lathouwers , Cornelius (Kees) Van Dun
发明人： Guy Weyens , Jean Lathouwers , Cornelius (Kees) Van Dun
IPC分类号： C12N15/82 , C12N15/54 , C12N15/11 , A01H5/00
CPC分类号： C12N15/8246 , C12P19/18
摘要： The present invention relates to fructan producing plants particularly to methods for the production of fructans having a low degree of polymerization. The invention is also directed to non-fructan producing plants which are transformed or crossed to contain at least two of three genes encoding a fructosyltransferase.
摘要翻译：本发明涉及果聚糖生产设备，特别涉及生产低聚合度的果聚糖的方法。本发明还涉及非果聚糖生产的植物，其转化或杂交以含有编码果糖基转移酶的三种基因中的至少两种。

8. 发明授权

US07241567B2 T227-1 flanking sequence 有权
标题翻译： T227-1侧翼序列
公开(公告)号：US07241567B2
公开(公告)日：2007-07-10
申请号：US10415602
申请日：2001-11-30
申请人： Guy Weyens , Steve Barnes , Inge Rosquin
发明人： Guy Weyens , Steve Barnes , Inge Rosquin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , C12N15/8275
摘要： A herbicide resistant transformed sugar beet that is detectable by the specific primers developed to match the DNA sequences that flank the left and/or right border region of the inserted transgenic DNA and the method of identifying primer pairs containing plant genomic DNA/plasmid DNA. More specifically the present invention covers a specific glyphosate resistant sugar beet plant having an insertion of the transgenic material identified as the T227-1 event. The present invention additionally covers primer pairs: plant genomic DNA/Plasmid DNA that are herein identified. Additionally, these primer pairs for either the left or the right flanking regions make an event specific test for the T227-1 insert of transgenic material.
摘要翻译：一种抗除草剂转化的甜菜，其可通过与所插入的转基因DNA的左侧和/或右侧边界区域侧翼的DNA序列匹配的特异性引物以及鉴定含有植物基因组DNA /质粒DNA的引物对的方法来检测。更具体地说，本发明涵盖具有被鉴定为T227-1事件的转基因材料插入的特异性抗草甘膦甜菜植物。本发明另外涵盖引物对：本文鉴定的植物基因组DNA /质粒DNA。另外，对于左侧或右侧翼区域的这些引物对对转基因材料的T227-1插入物进行事件特异性检验。

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