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1. 发明授权

US4498774A Micro-polarimeter for high performance liquid chromatography 失效
标题翻译：用于高效液相色谱的微型偏光仪
公开(公告)号：US4498774A
公开(公告)日：1985-02-12
申请号：US489031
申请日：1983-04-27
申请人： Edward E. Yeung , Larry E. Steenhoek , Steven D. Woodruff , Jeng-Chung Kuo
发明人： Edward E. Yeung , Larry E. Steenhoek , Steven D. Woodruff , Jeng-Chung Kuo
IPC分类号： G01N21/05 , G01N21/21 , G01N30/74 , G01N31/06
CPC分类号： G01N30/74 , G01N21/21 , G01N2030/746 , G01N21/05
摘要： A micro-polarimeter interfaced with a system for high performance liquid chromatography, for quantitatively analyzing micro and trace amounts of optically active organic molecules, particularly carbohydrates. A flow cell with a narrow bore is connected to a high performance liquid chromatography system. Thin, low birefringence cell windows cover opposite ends of the bore. A focused and polarized laser beam is directed along the longitudinal axis of the bore as an eluent containing the organic molecules is pumped through the cell. The beam is modulated by air gap Faraday rotators for phase sensitive detection to enhance the signal to noise ratio. An analyzer records the beams's direction of polarization after it passes through the cell. Calibration of the liquid chromatography system allows determination of the quantity of organic molecules present from a determination of the degree to which the polarized beam is rotated when it passes through the eluent.
摘要翻译：与用于高效液相色谱的系统接口的微旋光仪，用于定量分析微量和痕量的光学活性有机分子，特别是碳水化合物。具有窄孔的流动池连接到高效液相色谱系统。薄，低双折射单元窗口覆盖孔的相对端。聚焦和偏振的激光束沿着孔的纵向轴线引导，因为含有有机分子的洗脱液被泵送通过细胞。光束由气隙法拉第旋转器调制，用于相位敏感检测，以增强信噪比。分析仪在通过电池后记录光束的极化方向。液相色谱系统的校准允许通过确定偏振光束通过洗脱液时旋转的程度来确定存在的有机分子的量。

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