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    • 3. 发明申请
    • HIGH THROUGHPUT MULTIPLEX DNA SEQUENCE AMPLIFICATIONS
    • 高通量多重DNA序列扩增
    • WO2004033649A3
    • 2004-08-26
    • PCT/US0331874
    • 2003-10-07
    • UNIV NEW JERSEY MEDLI HONGHUALI JAMES
    • LI HONGHUALI JAMES
    • C12N20060101C12Q1/68G06F19/20
    • C12Q1/6869G06F19/20G06F19/22C12Q2537/143C12Q2531/113C12Q2525/185
    • The present invention provides methods of designing PCR primers that allow the efficient and simultaneous amplification of a large number of different desired DNA fragments in a single multiplex PCR and minimize the formation of nonspecific extensions of undesired DNA fragments. The present invention allows a multiplex PCRto use at least 50 pairs of primers and produce at least 50 DNA fragments of interest. The present invention significantly broadens the application of multiplex PCR in the identification of multiple genes related to multifactorial diseases, the genome-scale detection of genetic alterations, the studies in large-scale pharmacogenetic reactions, the genotyping genetic polymorphism in a large population, the gene expression profiling in various samples, and high throughput genotyping technologies.
    • 本发明提供了设计PCR引物的方法,该方法允许在单次多重PCR中有效且同时扩增大量不同的所需DNA片段,并使非期望DNA片段的非特异性延伸的形成最小化。 本发明允许多重PCR使用至少50对引物并产生至少50个感兴趣的DNA片段。 本发明显着拓宽了多重PCR在鉴定与多因子疾病有关的多个基因,遗传改变的基因组规模检测,大规模药物遗传学反应的研究,大量群体中的基因分型遗传多态性,基因 在各种样品中的表达谱分析以及高通量基因分型技术。