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    • 7. 发明申请
    • METHOD FOR LARGE SCALE MUTAGENESIS IN CROP PLANTS
    • 农作物大规模生产的方法
    • WO9912411A3
    • 1999-09-16
    • PCT/IL9800442
    • 1998-09-10
    • YEDA RES & DEVYISSUM RES DEV COLEVY AVRAHAM AMEISSNER RAFAELELKIND YONATAN
    • LEVY AVRAHAM AMEISSNER RAFAELELKIND YONATAN
    • A01H5/00A01H1/04C12N15/01C12N15/09C12N15/82
    • C12N15/8203A01H1/04C12N15/01C12N15/8201C12N15/8216C12N15/8241
    • The present invention enables the rapid and large-scale production of mutants in crop plants. This is accomplished by utilizing a miniature plant which can be crossed with a commercial plant of the same species. Mutations are induced in the miniature cultivar, and the mutants subsequently identified in the resulting mutant plant population. Mutant genes of interest can be introgressed into a commercial cultivar by crossing selected mutant miniature plants with the commercial cultivar. Reverse genetics can be undertaken using a plant population of the miniature crop which contains random T-DNA or transposon insertion events and screening this population for insertions into genes of interest. Likewise, the miniature plant population is transformed with a DNA construct comprised of a promoter-less screenable marker gene within a mobile DNA. The mutants derived from this construct are rapidly screened for expression of the screenable marker gene and the promoter operably linked to the screenable marker gene in these transformants is cloned.
    • 本发明能够在作物植物中快速大规模生产突变体。 这是通过利用可以与相同物种的商业植物杂交的微型植物来实现的。 在微型品种中诱导突变,随后在所得突变体植物种群中鉴​​定突变体。 通过将选定的突变体微型植物与商业栽培品种杂交,可以将目的突变基因渗入商业品种。 可以使用包含随机T-DNA或转座子插入事件的微型作物的植物种群进行反向遗传学,并筛选该群体以将其插入感兴趣的基因。 同样地,用移动DNA内的无启动子筛选标记基因的DNA构建体转化微型植物种群。 从该构建体衍生的突变体被快速筛选用于表达可筛选的标记基因,并且克隆了可操作地连接到这些转化子中的可筛选标记基因的启动子。