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1. 发明申请

WO2004065550A3 NUCLEIC ACID AMPLIFICATION USING NON-STANDARD BASES 审中-公开
标题翻译：使用非标准基的核酸扩增
公开(公告)号：WO2004065550A3
公开(公告)日：2004-12-23
申请号：PCT/US2004001397
申请日：2004-01-17
申请人： ERAGEN BIOSCIENCES INC , PRUDENT JAMES R , JOHNSON SCOTT C , MOSER MICHAEL J , MARSHALL DAVID J
发明人： PRUDENT JAMES R , JOHNSON SCOTT C , MOSER MICHAEL J , MARSHALL DAVID J
IPC分类号： C12N20060101 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6844 , C12Q2525/101
摘要： The present invention provides methods, kits and compositions for high fidelity nucleic acid amplification reactions of target nucleic acids such that specific amplification products that incorporate non-standard bases are produced. The amplification reactions can either be linear or exponential. In some embodiments, the fidelity of the amplification reaction is high enough such that a majority of the amplification products maintain non-standard bases at specific sites designated by the user after a specified number of amplification cycles. In some embodiments, the ratio of non-standard nucleoside triphosphates is greater than the amount of standard nucleoside triphosphates initially present in the reaction mixture.
摘要翻译：本发明提供用于靶核酸的高保真度核酸扩增反应的方法，试剂盒和组合物，从而产生掺入非标准碱基的特定扩增产物。扩增反应可以是线性的或指数的。在一些实施方案中，扩增反应的保真度足够高，使得大多数扩增产物在指定数量的扩增循环后在由用户指定的特定位点保持非标准碱基。在一些实施方案中，非标准三磷酸核苷的比例大于最初存在于反应混合物中的标准核苷三磷酸的量。

2. 发明申请

WO2004090153A3 POLYMERASE INHIBITOR AND METHOD OF USING SAME 审中-公开
标题翻译：聚合物抑制剂及其使用方法
公开(公告)号：WO2004090153A3
公开(公告)日：2005-06-23
申请号：PCT/US2004010029
申请日：2004-04-01
申请人： ERAGEN BIOSCIENCES INC , MOSER MICHAEL J , VAN HOUT CRISTOPHER V , LARSEN CHRISTINE A , MARSHALL DAVID J , PRUDENT JAMES R
发明人： MOSER MICHAEL J , VAN HOUT CRISTOPHER V , LARSEN CHRISTINE A , MARSHALL DAVID J , PRUDENT JAMES R
IPC分类号： C07H21/04 , C12N15/11 , C12Q20060101 , C12Q1/48
CPC分类号： C12Q1/48
摘要： The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.
摘要翻译：本发明提供了基于核酸的聚合酶抑制剂和用于在核酸扩增反应中减少非特异性聚合酶延伸和扩增的方法。聚合酶抑制剂提供双链核酸部分，其被聚合酶识别为扩增模板，但不能被聚合酶延伸。聚合酶结合聚合酶抑制剂，其将酶螯合，直到温度达到使抑制剂的双链部分变性的水平，其后聚合酶被释放，然后可以催化核酸延伸。

3. 发明申请

WO2008048334A3 MATERIALS AND METHODS FOR THE DETECTION OF ANTHRAX RELATED TOXIN GENES 审中-公开
标题翻译：用于检测蒽衍生毒素基因的材料和方法
公开(公告)号：WO2008048334A3
公开(公告)日：2008-11-20
申请号：PCT/US2007000738
申请日：2007-01-11
申请人： ERAGEN BIOSCIENCES INC , MOSER MICHAEL J , MARSHALL DAVID J
发明人： MOSER MICHAEL J , MARSHALL DAVID J
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Disclosed are methods and kits for identifying a virulent bacteria in a sample, which may include virulent bacteria belonging to the Bacillus genus (e.g., Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis). Typically, the methods include (a) reacting a mixture that includes, in addition to nucleic acid isolated from the sample, (i) at least one oligonucleotide capable of specifically hybridizing to nucleic acid of plasmid pX01; and (ii) at least one oligonucleotide capable of specifically hybridizing to nucleic acid of plasmid pX02. In addition, the mixture may include control nucleic acid. In the methods, nucleic acid of plasmid pX01 and nucleic acid of plasmid pX02 are detected, and optionally control nucleic acid is detected, thereby identifying the virulent bacteria.
摘要翻译：公开了用于鉴定样品中毒性细菌的方法和试剂盒，其可以包括属于芽孢杆菌属的毒性细菌（例如炭疽芽孢杆菌，蜡状芽孢杆菌和苏云金芽孢杆菌）。典型地，所述方法包括（a）使除了从样品分离的核酸之外还包含（i）至少一种能够与质粒pX01的核酸特异性杂交的寡核苷酸的混合物反应; 和（ii）至少一种能够与质粒pX02的核酸特异性杂交的寡核苷酸。另外，该混合物可以包含对照核酸。在该方法中，检测质粒pX01的核酸和质粒pX02的核酸，并任选检测对照核酸，从而鉴定有毒细菌。

4. 发明申请

WO0190417A3 MATERIALS AND METHODS FOR DETECTION OF NUCLEIC ACIDS 审中-公开
标题翻译：用于检测核酸的材料和方法
公开(公告)号：WO0190417A3
公开(公告)日：2003-08-07
申请号：PCT/US0116359
申请日：2001-05-18
申请人： ERAGEN BIOSCIENCES INC
发明人： PTACIN JEROD L , MARSHALL DAVID J , PRUDENT JAMES R , SCHERRILL CHRISTOPHER B , SHAPIRO GIDEON , GRENIER JENNIFER K , RICHMOND CRAIG S , JURCZK SIMONA
IPC分类号： G01N21/64 , C12N15/09 , C12Q1/48 , C12Q1/68 , G01N21/78 , G01N33/483 , G01N33/53 , G01N33/566 , G01N33/58
CPC分类号： C12Q1/6853 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6834 , C12Q1/6858 , C12Q2600/156 , C12Q2565/1015 , C12Q2525/101 , C12Q2525/161
摘要： Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; cleaving, after annealing, at least a portion of the reporter to release at least one reporter fragment; and correlating the release of the at least one reporter fragment with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample. Alternatively, the reporter can be incorporated into the amplification product rather than annealing and then cleaving.
摘要翻译：描述了使用非天然碱的测定法。在一个实施方案中，所述方法包括使怀疑含有靶核酸的样品与聚合酶和第一和第二引物接触; 如果存在于样品中，则使用第一和第二引物通过PCR扩增靶核酸以产生具有双链区域和包含非天然碱基的单链区域的扩增产物; 使样品与包含与单链区域的非天然碱基互补的标记物和非天然碱基的报告子接触; 将至少一部分报告物退火至扩增产物的单链区域; 在退火后切割至少一部分报告物以释放至少一个报道片段; 并将至少一个报道片段的释放与样品中靶核酸的存在相关联。本发明还提供用于检测样品中靶核酸的相应试剂盒。或者，可以将报道子并入扩增产物，而不是退火然后裂解。

5. 发明申请

WO0233126A3 SOLID SUPPORT ASSAY SYSTEMS AND METHODS UTILIZING NON-STANDARD BASES 审中-公开
标题翻译：固体支持测定系统和使用非标准基础的方法
公开(公告)号：WO0233126A3
公开(公告)日：2003-08-28
申请号：PCT/US0131993
申请日：2001-10-15
申请人： ERAGEN BIOSCIENCES INC
发明人： GRENIER JENNIFER K , MARSHALL DAVID J , PRUDENT JAMES R , RICHMOND CRAIG S , ROESCH ERIC B , SCHERRER CHRISTOPHER W , SHERRILL CHRISTOPHER B , PTACIN JEROD L
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/6832 , C12Q1/6823 , C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2600/156 , C12Q2565/1015 , C12Q2525/101 , C12Q2525/161 , C12Q2525/117 , C12Q2565/537
摘要： Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide the solid support. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorpoaration of a non-standard base (e.g., via PCR or ligation) is used in the assay.
摘要翻译：描述了使用非标准碱的固体支持试验。将包含分子识别序列的捕获寡核苷酸连接到固体支持物上，并与固体支持物的靶寡核苷酸杂交。在一些情况下，分子识别序列包括一个或多个非标准碱基并与靶寡核苷酸的互补标记序列杂交。在其他情况下，在测定中使用非标准碱基的引入（例如通过PCR或连接）。

6. 发明专利

AU2004206246A1 Nucleic acid amplification using non-standard bases 未知
公开(公告)号：AU2004206246A1
公开(公告)日：2004-08-05
申请号：AU2004206246
申请日：2004-01-17
申请人： ERAGEN BIOSCIENCES INC
发明人： PRUDENT JAMES R , JOHNSON SCOTT C , MOSER MICHAEL J , MARSHALL DAVID J
IPC分类号： C12N20060101 , C12P19/34 , C12Q1/68

7. 发明专利

AU1317502A Solid support assay systems and methods utilizing non-standard bases 未知
公开(公告)号：AU1317502A
公开(公告)日：2002-04-29
申请号：AU1317502
申请日：2001-10-15
申请人： ERAGEN BIOSCIENCES INC
发明人： GRENIER JENNIFER K , MARSHALL DAVID J , PRUDENT JAMES R , RICHMOND CRAIG S , ROESCH ERIC B , SCHERRER CHRISTOPHER W , SHERRILL CHRISTOPHER B , PTACIN JEROD L
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566

8. 发明专利

CA2425747A1 SOLID SUPPORT ASSAY SYSTEMS AND METHODS UTILIZING NON-STANDARD BASES 未知
公开(公告)号：CA2425747A1
公开(公告)日：2002-04-25
申请号：CA2425747
申请日：2001-10-15
申请人： ERAGEN BIOSCIENCES INC
发明人： PTACIN JEROD L , SCHERRER CHRISTOPHER W , SHERRILL CHRISTOPHER B , MARSHALL DAVID J , ROESCH ERIC B , RICHMOND CRAIG S , GRENIER JENNIFER K , PRUDENT JAMES R
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566
摘要： Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide the solid support . In some instances, the molecular recognition sequence includes one or more n on- standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorpoaration of a non-standard base (e.g., via PCR or ligation) is used in the assay.

9. 发明专利

AT497018T 未知
公开(公告)号：AT497018T
公开(公告)日：2011-02-15
申请号：AT01950231
申请日：2001-05-18
申请人： ERAGEN BIOSCIENCES INC
发明人： MARSHALL DAVID J , PRUDENT JAMES R , SCHERRILL CHRISTOPHER B , SHAPIRO GIDEON , GRENIER JENNIFER K , RICHMOND CRAIG S , JURCZYK SIMONA , PTACIN JEROD L
IPC分类号： C12Q1/68 , G01N21/64 , C12N15/09 , C12Q1/48 , G01N21/78 , G01N33/483 , G01N33/53 , G01N33/566 , G01N33/58

10. 发明专利

AU2004227353A1 Polymerase inhibitor and method of using same 未知
公开(公告)号：AU2004227353A1
公开(公告)日：2004-10-21
申请号：AU2004227353
申请日：2004-04-01
申请人： ERAGEN BIOSCIENCES INC
发明人： LARSEN CHRISTINE A , MOSER MICHAEL J , PRUDENT JAMES R , HOUT CRISTOPHER V VAN , MARSHALL DAVID J
IPC分类号： C07H21/04 , C12N15/11 , C12Q20060101 , C12Q1/48
摘要： The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.

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