专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO9617940A2 METHOD OF DESULFURIZATION OF FOSSIL FUEL WITH FLAVOPROTEIN 审中-公开
标题翻译： FASIL燃料与FLAVOPROTEIN的脱硫方法
公开(公告)号：WO9617940A2
公开(公告)日：1996-06-13
申请号：PCT/US9515865
申请日：1995-12-05
申请人： ENERGY BIOSYSTEMS CORP , SQUIRES CHARLES H , JI WAN , XI LEI , ORTEGO BEATRICE C , POGREBINSKY OLGA S , GRAY KEVIN A , CHILDS JOHN D
发明人： SQUIRES CHARLES H , JI WAN , XI LEI , ORTEGO BEATRICE C , POGREBINSKY OLGA S , GRAY KEVIN A , CHILDS JOHN D
IPC分类号： C12N15/09 , C10G32/00 , C10L1/04 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/53 , C12P1/00 , C12R1/01 , C12R1/19 , C12S1/02 , C12N15/52
CPC分类号： C12N9/0036 , C10G32/00 , C12P1/00 , C12R1/01
摘要： The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.
摘要翻译：本发明涉及通过向生物催化剂中加入黄素蛋白来提高化石燃料脱硫反应速率的发现。本发明涉及提高含有机硫化合物的化石燃料脱硫速率的方法，包括以下步骤：a）使化石燃料与含有能够裂解碳 - 硫键的生物催化剂的水相接触，增加量的黄素蛋白，从而形成化石燃料和水相混合物; b）将步骤（a）的混合物保持在足以通过生物催化剂裂解有机硫分子的碳 - 硫键的条件下，从而导致具有降低的有机硫含量的化石燃料; 和c）从所得水相中分离具有降低的有机硫含量的化石燃料。本发明还涉及含有一种或多种重组DNA分子的重组微生物，所述重组DNA分子编码能够脱硫含有有机硫分子的化石燃料并且编码黄素蛋白的生物催化剂。本发明还涉及包含（a）能够使含有有机硫分子的化石燃料脱硫的生物催化剂和（b）黄素蛋白的组合物。

2. 发明专利

BR9510082A 未知
公开(公告)号：BR9510082A
公开(公告)日：1998-07-14
申请号：BR9510082
申请日：1995-12-05
申请人： ENERGY BIOSYSTEMS CORP
发明人： JI WAN , SQUIRES CHARLES H , XI LEI , POGREBINSKY OLGA S , ORTEGO BEATRICE C , CHILDS JOHN D , GRAY KEVIN A
IPC分类号： C12N15/09 , C10G32/00 , C10L1/04 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/53 , C12P1/00 , C12R1/01 , C12R1/19 , C12S1/02 , C12N15/52
摘要： The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.

3. 发明专利

AU2779399A A gene involved in thiophene degradation from (nocardia asteroides) kgb1 未知
公开(公告)号：AU2779399A
公开(公告)日：1999-09-15
申请号：AU2779399
申请日：1999-02-22
申请人： ENERGY BIOSYSTEMS CORP
发明人： WANG YONGZHAO , CHILDS JOHN D , SQUIRES CHARLES H
IPC分类号： C10G32/00 , C12N9/02 , C12N15/53 , C12P1/06 , C12P3/00 , C12S1/02 , C12N15/70
摘要： The present invention relates to an isolated nucleic acid molecule which encodes one or more of the enzymes which catalyze one or more steps in the desulfurization of thiophene, or a homologue or active fragment thereof. The invention also includes a recombinant microorganism containing one or more such heterologous nucleic acid molecules. The invention also provides a method for desulfurizing a fossil fuel containing thiophene and/or one or more substituted thiophenes. The method comprises contacting the fossil fuel with an organism containing a recombinant nucleic acid molecule which encodes an enzyme which catalyzes the desulfurization of thiophene.

4. 发明专利

AU4990097A A (rhodococcus) flavin reductase and its use in desulfurization of fossil fuel 未知
公开(公告)号：AU4990097A
公开(公告)日：1998-05-15
申请号：AU4990097
申请日：1997-10-22
申请人： ENERGY BIOSYSTEMS CORP
发明人： GRAY KEVIN A , CHILDS JOHN D , SQUIRES CHARLES H
IPC分类号： C10G32/00 , C12N9/02
摘要： The invention provides a novel flavin reductase, which has been isolated from Rhodococcus strain sp. IGTS8 and purified to homogeneity. The flavin reductase is of use in the biocatalytic desulfurization of a fossil fuel containing organic sulfur compounds. The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of the flavin reductase of the present invention, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.

5. 发明专利

MX9704165A METODO DE DESULFURACION DE COMBUSTIBLE FOSIL CON FLAVOPROTEINA. 未知
公开(公告)号：MX9704165A
公开(公告)日：1997-09-30
申请号：MX9704165
申请日：1995-12-05
申请人： ENERGY BIOSYSTEMS CORP
发明人： SQUIRES CHARLES H , JI WAN , XI LEI , ORTEGO BEATRICE C , POGREBINSKY OLGA S , GRAY KEVIN A , CHILDS JOHN D
IPC分类号： C12N15/09 , C10G32/00 , C10L1/04 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/53 , C12P1/00 , C12R1/01 , C12R1/19 , C12S1/02 , C12S01/02 , C12N09/02 , C12N01/21 , C12N15/52
摘要： La invencion se refiere al descubrimiento de que el índice de reaccion de la desulfurizacion de combustibles fosiles se incrementa con la adicion de una flavoproteína al biocatalizador. La invencion se refiere a un método que incrementa el índice de desulfurizacion de un combustible fosil que contenga compuestos de azufre orgánico, que comprende las siguientes etapas: a) contactar el combustible fosil con una fase acuosa que contenga un blocatalizador capaz de desdoblar enlaces de azufre-carbono, y un cantidad de flavoproteína que incrementa elíndice, de donde se forma un combustible fosil y una mezcla de fase acuosa; b) mantener la mezcla de la etapa (a) bajo condiciones suficientes para el desdoblamiento de los enlaces de azufre-carbono de las moléculas de azufre orgánico por medio del biocatalizador, de ahí que resulta un combustible fosil qua tiene un contenido reducido de azufre orgánico; y c) separar de la fase acuosa resultante el combustible fosil que tiene un contenido reducido de azufre orgánico. La invencion también se refiere a un microorganismo recombinante que contiene una o más moléculas de ADN recombinantes que codifican un biocatalizador capaz de desulfurizar un combustible fosil que contenga moléculas de azufre orgánico, y que codifican una flavoproteína. Asimismo, la invencion se refiere a una composicion que comprende: (a) un biocatalizador capaz de desulfurizar un combustible fosil que contenga moléculas de azufre orgánico, y (b) una flavoproteína.

6. 发明专利

AU6950698A Dsz gene expression in (pseudomonas) hosts 未知
公开(公告)号：AU6950698A
公开(公告)日：1998-10-30
申请号：AU6950698
申请日：1998-04-03
申请人： ENERGY BIOSYSTEMS CORP
发明人： DARZINS ALDIS , XI LEI , CHILDS JOHN D , MONTICELLO DANIEL J , SQUIRES CHARLES H
IPC分类号： C10G32/00 , C12N1/21 , C12N9/02 , C12N15/52 , C12S1/02 , C12P11/00
摘要： The present invention provides novel recombinant pseudomonads which contain a heterologous nucleic acid molecule comprising a nucleotide sequence encoding one or more desulfurization enzymes which are components of a biodesulfurization catalyst. The invention also provides a method of desulfurizing a carbonaceous material, such as a fossil fuel, which comprises organosulfur compounds. The method includes the steps of The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a recombinant biocatalyst which is capable of cleaving carbon-sulfur bonds and, optionally, a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organosulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.

7. 发明专利

AU693633B2 Method of desulfurization of fossil fuel with flavoprotein 未知
公开(公告)号：AU693633B2
公开(公告)日：1998-07-02
申请号：AU4375596
申请日：1995-12-05
申请人： ENERGY BIOSYSTEMS CORP
发明人： SQUIRES CHARLES H , JI WAN , XI LEI , ORTEGO BEATRICE C , POGREBINSKY OLGA S , GRAY KEVIN A , CHILDS JOHN D
IPC分类号： C12N15/09 , C10G32/00 , C10L1/04 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/53 , C12P1/00 , C12R1/01 , C12R1/19 , C12S1/02 , C12N15/52
摘要： The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.

8. 发明专利

AU4375596A Method of desulfurization of fossil fuel with flavoprotein 未知
公开(公告)号：AU4375596A
公开(公告)日：1996-06-26
申请号：AU4375596
申请日：1995-12-05
申请人： ENERGY BIOSYSTEMS CORP
发明人： SQUIRES CHARLES H , JI WAN , XI LEI , ORTEGO BEATRICE C , POGREBINSKY OLGA S , GRAY KEVIN A , CHILDS JOHN D
IPC分类号： C12N15/09 , C10G32/00 , C10L1/04 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/53 , C12P1/00 , C12R1/01 , C12R1/19 , C12S1/02 , C12N15/52
摘要： The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.

9. 发明专利

CA2206987A1 METHOD OF DESULFURIZATION OF FOSSIL FUEL WITH FLAVOPROTEIN 未知
公开(公告)号：CA2206987A1
公开(公告)日：1996-06-13
申请号：CA2206987
申请日：1995-12-05
申请人： ENERGY BIOSYSTEMS CORP
发明人： SQUIRES CHARLES H , JI WAN , ORTEGO BEATRICE C , XI LEI , GRAY KEVIN A , POGREBINSKY OLGA S , CHILDS JOHN D
IPC分类号： C12N15/09 , C10G32/00 , C10L1/04 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/53 , C12P1/00 , C12R1/01 , C12R1/19 , C12S1/02 , C12N15/52
摘要： The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式