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1. 发明授权

US10435699B2 Methods and compositions for generating complex trait loci 有权
公开(公告)号：US10435699B2
公开(公告)日：2019-10-08
申请号：US13748704
申请日：2013-01-24
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY
发明人： Saverio Carl Falco , Michael W Lassner , Zhongsen Li , Christopher Jay Scelonge
IPC分类号： C12N15/82 , C12N9/16 , C12N9/22
摘要： Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.

2. 发明申请

US20130252242A1 SOYBEAN EVENT 3560.4.3.5 AND COMPOSITIONS AND METHODS FOR THE IDENTIFICATION AND/OR DETECTION THEREOF 审中-公开
标题翻译：大豆事件3560.4.3.5及其鉴定和/或检测的组合物和方法
公开(公告)号：US20130252242A1
公开(公告)日：2013-09-26
申请号：US13909641
申请日：2013-06-04
申请人： E.I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： Robert F. Cressman, JR. , Nancy L. Henderson , David Hondred , Nancy J. Leysens , Zhongsen Li , Natalie N. Weber , Aiqiu Xing , Cathy Xiaoyan Zhong
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , A01H5/10 , C12N15/8274 , C12N15/8275 , C12N15/8278
摘要： Compositions and methods related to transgenic glyphosate/ALS inhibitor-tolerant soybean plants are provided. Specifically, soybean plants having a 3560.4.3.5 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide are provided. The soybean plant harboring the 3560.4.3.5 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:10 and/or 11. The characterization of the genomic insertion site of the 3560.4.3.5 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean 3560.4.3.5 events are provided.
摘要翻译：提供了与转基因草甘膦/ ALS抑制剂耐受性大豆植物相关的组合物和方法。具体地说，提供了赋予对草甘膦和至少一种ALS抑制性除草剂耐受性的3560.4.3.5事件的大豆植物。在所述染色体位置处携带3560.4.3.5事件的大豆植物包含至少具有SEQ ID NO：10和/或11的多核苷酸序列的基因组/转基因接头.3560.4.3.5事件的基因组插入位点的表征提供为了提高繁殖效率，并且能够使用分子标记来跟踪繁殖种群中的转基因插入物及其后代。提供了用于鉴定，检测和使用大豆3560.4.3.5事件的各种方法和组合物。

3. 发明申请

US20130198888A1 METHODS AND COMPOSITIONS FOR GENERATING COMPLEX TRAIT LOCI 审中-公开
标题翻译：用于产生复杂TRAIT LOCI的方法和组合物
公开(公告)号：US20130198888A1
公开(公告)日：2013-08-01
申请号：US13748704
申请日：2013-01-24
申请人： Pioneer Hi-Bred International, Inc. , E.I. Du Pont De Nemours And Company
发明人： Carl Falco , Michael Lassner , Zhongsen Li , Christopher Scelonge
IPC分类号： A01H5/10 , A01H1/02
CPC分类号： C12N15/8213 , C12N9/16 , C12N9/22
摘要： Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.
摘要翻译：提供组合物和方法用于将多个独立的转基因基因座堆叠到植物的基因组中。组合物包括植物，种子或植物细胞，其包含至少一个转基因靶位点和至少一个目的基因组集成在基因组窗口内的不同基因组位点。可以使用植物育种技术，使得转基因靶位点和感兴趣的基因组座位可以一起繁殖。以这种方式，可以在基因组窗口内产生多个独立的转基因整合以产生复杂的性状基因座。复杂特征位点的设计使得目的基因座和/或基因座位点彼此独立地分离，从而提供通过繁殖和遗传特异性元件改变复杂性状基因座的益处。也可以使用各种方法来修饰靶位点，使得它们含有多种感兴趣的多核苷酸。

4. 发明申请

US20160032297A1 METHODS FOR THE IDENTIFICATION OF VARIANT RECOGNITION SITES FOR RARE-CUTTING ENGINEERED DOUBLE-STRAND-BREAK-INDUCING AGENTS AND COMPOSITIONS AND USES THEREOF 审中-公开
标题翻译：用于识别用于稀释工程双重断裂诱导剂及其组合物的变体识别站点的方法及其用途
公开(公告)号：US20160032297A1
公开(公告)日：2016-02-04
申请号：US14775777
申请日：2014-03-10
申请人： E.I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： Stephane Deschamps , James English , Zhongsen Li , Victor Llaca , Joshua K. Young
IPC分类号： C12N15/82 , C12N9/22
CPC分类号： C12N15/8213 , C12N9/22 , C12N15/8241 , C12Q1/6811 , C12Q2521/313 , C12Q2525/191 , C12Q2521/507
摘要： Methods for the identification of variant recognition sites for rare cutting engineered double strand break inducing agents and compositions thereof are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the of variant recognition sites. Various methods of identifying variant recognition sites with increased substrate activity for a rare cutting engineered double strand break inducing agents are provided.
摘要翻译：提供了用于鉴定稀有切割工程双链断裂诱导剂的变体识别位点的方法及其组合物。还提供了具有变异识别位点的核酸构建体，酵母，植物，植物细胞，外植体，种子和谷物。提供了用于鉴定具有增加的稀有切割工程双链断裂诱导剂的底物活性的变异识别位点的各种方法。

5. 发明申请

US20130053246A1 SOYBEAN EVENT 3560.4.3.5 AND COMPOSITIONS AND METHODS FOR THE IDENTIFICATION AND/OR DETECTION THEREOF 审中-公开
标题翻译：大豆事件3560.4.3.5及其鉴定和/或检测的组合物和方法
公开(公告)号：US20130053246A1
公开(公告)日：2013-02-28
申请号：US13658388
申请日：2012-10-23
申请人： Pioneer Hi-Bred International, Inc. , E.I. du Pont de Nemours and Company
发明人： Anthony D. Guida, JR. , Christine B. Hazel , Jeffrey M. Hegstad , Zhongsen Li , Mark D. Vogt , Aiqiu Xing
IPC分类号： A01N57/20 , A01P13/00
CPC分类号： A01H5/10 , C12N15/8274 , C12N15/8275 , C12N15/8278 , C12Q1/6895
摘要： Compositions and methods related to transgenic glyphosate/ALS inhibitor-tolerant soybean plants are provided. Specifically, soybean plants having a 3560.4.3.5 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide are provided. The soybean plant harboring the 3560.4.3.5 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:10 and/or 11. The characterization of the genomic insertion site of the 3560.4.3.5 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean 3560.4.3.5 events are provided. Methods and compositions for increasing yield are also provided.
摘要翻译：提供了与转基因草甘膦/ ALS抑制剂耐受性大豆植物相关的组合物和方法。具体地说，提供了赋予对草甘膦和至少一种ALS抑制性除草剂耐受性的3560.4.3.5事件的大豆植物。在所述染色体位置处携带3560.4.3.5事件的大豆植物包含至少具有SEQ ID NO：10和/或11的多核苷酸序列的基因组/转基因接头.3560.4.3.5事件的基因组插入位点的表征提供为了提高繁殖效率，并且能够使用分子标记来跟踪繁殖种群中的转基因插入物及其后代。提供了用于鉴定，检测和使用大豆3560.4.3.5事件的各种方法和组合物。还提供了用于提高产量的方法和组合物。

6. 发明申请

US20160017346A1 SOYBEAN AGB1 PROMOTER AND ITS USE IN TISSUE-SPECIFIC EXPRESSION OF TRANSGENIC GENES IN PLANTS 有权
标题翻译：大豆AGB1促销剂及其在植物特异性表达转基因中的应用
公开(公告)号：US20160017346A1
公开(公告)日：2016-01-21
申请号：US14772729
申请日：2014-03-12
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： Zhongsen Li
IPC分类号： C12N15/82
CPC分类号： C12N15/823 , C07K14/415
摘要： The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean predicted allergen Gly m Bd 28K peptide gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-specific manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
摘要翻译：本发明涉及大豆的基因表达调控序列，特别涉及大豆预测变应原Gly m Bd 28K肽基因及其片段的启动子及其在促进一种或多种异源核酸片段以组织特异性方式表达的用途在植物中。本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，转基因植物和含有重组构建体的启动子的种子，以及制备和使用该重组构建体的方法。

7. 发明申请

US20150376631A1 SOYBEAN HRP1 PROMOTER AND ITS USE IN TISSUE-SPECIFIC EXPRESSION OF TRANSGENIC GENES IN PLANTS 有权
标题翻译：大豆HRP1促进剂及其在植物转基因组织特异性表达中的应用
公开(公告)号：US20150376631A1
公开(公告)日：2015-12-31
申请号：US14772711
申请日：2014-03-12
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： Zhongsen Li
IPC分类号： C12N15/82
CPC分类号： C12N15/823 , C12N15/8234
摘要： The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean hypersensitive-induced response protein gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-specific manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
摘要翻译：本发明涉及大豆，特别是大豆过敏诱导的应答蛋白基因及其片段的启动子的基因表达调控序列及其在植物中以组织特异性方式促进一种或多种异源核酸片段的表达的用途。本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，转基因植物和含有重组构建体的启动子的种子，以及制备和使用该重组构建体的方法。

8. 发明申请

US20150184174A1 SOYBEAN ADF1 PROMOTER AND ITS USE IN CONSTITUTIVE EXPRESSION OF TRANSGENIC GENES IN PLANTS 有权
标题翻译：大豆ADF1促销剂及其在植物中转基因表达的表达
公开(公告)号：US20150184174A1
公开(公告)日：2015-07-02
申请号：US14418291
申请日：2013-08-07
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： Zhongsen Li
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8257 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/8286
摘要： The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean actin depolymerizing factor gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a constitutive manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
摘要翻译：本发明涉及大豆，特别是大豆肌动蛋白解聚因子基因及其片段的启动子的基因表达调控序列及其在植物中以组成型促进一种或多种异源核酸片段的表达的用途。本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，转基因植物和含有重组构建体的启动子的种子，以及制备和使用该重组构建体的方法。

9. 发明授权

US09556447B2 Soybean HRP1 promoter and its use in tissue-specific expression of transgenic genes in plants 有权
标题翻译：大豆HRP1启动子及其在植物转基因基因组织特异性表达中的应用
公开(公告)号：US09556447B2
公开(公告)日：2017-01-31
申请号：US14772711
申请日：2014-03-12
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： Zhongsen Li
IPC分类号： C12N15/82
CPC分类号： C12N15/823 , C12N15/8234
摘要： The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean hypersensitive-induced response protein gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-specific manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
摘要翻译：本发明涉及大豆，特别是大豆过敏诱导的应答蛋白基因及其片段的启动子的基因表达调控序列及其在植物中以组织特异性方式促进一种或多种异源核酸片段的表达的用途。本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，转基因植物和含有重组构建体的启动子的种子，以及制备和使用该重组构建体的方法。

10. 发明授权

US11788097B2 Methods and compositions for generating complex trait loci 有权
公开(公告)号：US11788097B2
公开(公告)日：2023-10-17
申请号：US17699260
申请日：2022-03-21
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , Corteva Agriscience LLC
发明人： Saverio Carl Falco , Michael Lassner , Zhongsen Li , Christopher J Scelonge
IPC分类号： C12N15/82 , C12N9/22 , C12N9/16
CPC分类号： C12N15/8213 , C12N9/22 , C12N9/16
摘要： Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.

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