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    • 3. 发明公开
    • COSMID VECTOR FOR PLANT TRANSFORMATION AND USE THEREOF
    • COSMIDVEKTOR ZUR TRANSFORMATION VON PFLANZEN UND VERWENDUNG DAVON
    • EP2042602A1
    • 2009-04-01
    • EP07767526.2
    • 2007-06-25
    • Japan Tobacco, Inc.
    • TAKAKURA, YoshimitsuKOMARI, ToshihikoISHIDA, YujiKOMORI, ToshiyukiHIEI, YukohMINE, ToshikiIMAYAMA, Teruyuki
    • C12N15/82A01H5/00C12N1/21C12N5/10
    • C12N15/82C12N15/8205
    • The present invention aims to provide novel vectors for plant transformation.
      The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that :
      1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups;
      2) they contain the trfA1 gene of an IncP plasmid;
      3) they contain an oriT of an IncP plasmid;
      4) they contain the incC1 gene of an IncP plasmid;
      5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA;
      6) they contain a drug resistance gene expressed in E.coli and a bacterium of the genus Agrobacterium;
      7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium;
      8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium;
      9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and
      10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.
    • 本发明旨在提供用于植物转化的新型载体。 本发明的载体是全长为15kb或更小的粘粒载体,其特征在于:1)它们含有IncP质粒的复制起点,但不包含其他质粒组的任何复制起点; 2)它们含有IncP质粒的trfA1基因; 3)它们含有一个IncP质粒的oriT; 4)它们含有IncP质粒的incC1基因; 5)它们含有λ噬菌体的cos位点,cos位点位于T-DNA外部; 6)它们含有在大肠杆菌中表达的耐药性基因和农杆菌属的细菌; 7)它们含有农杆菌属细菌的T-DNA右边界序列; 8)它们含有农杆菌属细菌的T-DNA左边界序列; 9)它们含有位于7)和8之间的植物转化的选择标记基因并在植物中表达; 和10)它们含有位于7)和8之间的限制性内切核酸酶识别位点用于克隆外来基因。
    • 5. 发明公开
    • AGROBACTERIUM BACTERIUM TO BE USED IN PLANT TRANSFORMATION METHOD
    • 土耳其林业公司土耳其语土耳其语土耳其语土耳其语芬兰语
    • EP2980214A1
    • 2016-02-03
    • EP14773265.5
    • 2014-03-27
    • JAPAN TOBACCO INC.
    • IMAYAMA, TeruyukiHIEI, YukohISHIDA, Yuji
    • C12N15/09A01H1/00A01H5/00C12N1/21
    • C12N15/8205
    • This invention relates to the Agrobacterium bacterium to be used in plant transformation method comprising three types of plasmids. The Agrobacterium bacterium of this invention comprises plasmids of (1) to (3) shown below:
      (1) a plasmid comprising the following components:
      (i) the virB gene, the virC gene, the virD1 gene, the virD2 gene, the virD3 gene, the virG gene and the virJ gene of pTiBo542, and
      (ii) an origin of replication;

      (2) a disarmed Ti plasmid or a disarmed Ri plasmid of Agrobacterium bacterium; and
      (3) a plasmid having a T-DNA region consisting of a desired DNA;
      wherein each of the plasmids of (1) to (3) has a replication mechanism that enables a mutual coexistence with each other.
    • 本发明涉及用于包含三种质粒的植物转化方法的农杆菌属细菌。 本发明的农杆菌属细菌包含下述(1)〜(3)的质粒:(1)包含以下成分的质粒:(i)virB基因,virC基因,virD1基因,virD2基因,virD3 基因,virG基因和pTiBo542的virJ基因,和(ii)复制起点; (2)解毒的Ti质粒或农杆菌细菌的解除的Ri质粒; 和(3)具有由所需DNA组成的T-DNA区的质粒; 其中(1)至(3)的每个质粒具有使彼此相互共存的复制机制。