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2. 发明申请

US20130040390A1 CULTURE MEDIUM SUPPLEMENT AND USE THEREOF 审中-公开
标题翻译：文化媒介补充及其使用
公开(公告)号：US20130040390A1
公开(公告)日：2013-02-14
申请号：US13263865
申请日：2010-07-29
申请人： Duanqing Pei , Jiekai Chen , Jing Liu
发明人： Duanqing Pei , Jiekai Chen , Jing Liu
IPC分类号： C12N5/071
CPC分类号： C12N5/0696 , C12N2500/38 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/33 , C12N2501/603 , C12N2501/727 , C12N2501/999 , C12N2506/1307 , C12N2510/00
摘要： The present invention provides a chemically defined culture system, by which induced pluripotent stem (iPS) cells are obtained with high efficiency. The culture medium supplement of the present invention includes vitamin C and a glycogen synthase kinase-3 inhibitor; another culture medium supplement of the present invention further includes, in addition to vitamin C and the glycogen synthase kinase-3 inhibitor, vitamin B12, insulin, a receptor tyrosine kinase, and an anti-oxidant; and the culture medium supplement of the present invention may further be a mixture of the above two culture medium supplements with a serum replacement cell growth promoter. The present invention further provides a complete culture medium for iPS cells, which is formed by one or more of a basal culture medium, serum, and a serum replacement supplement, and the above culture medium supplements, or formed only by the above culture medium supplements and a basal culture medium. The culture system of the present invention is a chemically defined culture system free of serum and contamination from animal source, by which the iPS cells are obtained with high efficiency. The culture system maintains the growth and proliferation of the cells in conversion of somatic cells to iPS cells in absence of feeder cells, significantly accelerates the iPS cell induction process, and greatly improves the induction efficiency of somatic cells into iPS cells.
摘要翻译：本发明提供一种化学上定义的培养系统，通过该培养系统以高效率获得诱导的多能干细胞（iPS）细胞。本发明的培养基补充剂包括维生素C和糖原合酶激酶-3抑制剂; 除了维生素C和糖原合酶激酶-3抑制剂之外，本发明的另一种培养基补充剂还包括维生素B12，胰岛素，受体酪氨酸激酶和抗氧化剂; 并且本发明的培养基补充物还可以是上述两种培养基补充物与血清替代细胞生长促进剂的混合物。本发明还提供一种用于iPS细胞的完整培养基，其由一种或多种基础培养基，血清和血清替代物补充剂形成，并且上述培养基补充剂或仅由上述培养基补充剂形成和基础培养基。本发明的培养系统是不含动物源的血清和污染的化学上定义的培养系统，通过该培养系统以高效率获得iPS细胞。培养体系保持细胞在不存在饲养细胞时体细胞转化为iPS细胞的生长和增殖，显着加速iPS细胞诱导过程，并大大提高体细胞诱导iPS细胞的诱导效率。

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