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2. 发明授权

EP2847338B1 METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETED INTEGRATION OF TRANSGENES 有权转让
公开(公告)号：EP2847338B1
公开(公告)日：2018-09-19
申请号：EP13788413.6
申请日：2013-05-07
申请人： Sangamo Therapeutics, Inc. , Dow AgroSciences LLC
发明人： COST, Gregory, J. , URNOV, Fyodor , AINLEY, William M. , PETOLINO, Joseph F. , SAMUEL, Jayakumar Pon , WEBB, Steven R. , SASTRY-DENT, Lakshmi
IPC分类号： C12N15/87 , C12N15/00 , C12N5/10 , C12N15/82 , C12N15/10 , C12N15/85
CPC分类号： C12N15/8201 , C12N15/102 , C12N15/8209 , C12N15/8213 , C12N15/8216 , C12N15/8241 , C12N15/8247 , C12N15/8274 , C12N15/8278 , C12N15/8286 , C12N15/85 , C12N15/87 , C12N2800/80 , Y02A40/162
摘要： Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.

3. 发明授权

EP3070169B1 OPTIMIZED NON-CANONICAL ZINC FINGER PROTEINS 有权
标题翻译：优化的非正则锌指蛋白
公开(公告)号：EP3070169B1
公开(公告)日：2018-05-09
申请号：EP16163183.3
申请日：2007-12-13
申请人： Dow AgroSciences, LLC , Sangamo Therapeutics, Inc.
发明人： CAI, Qihua C. , MILLER, Jeffrey , URNOV, Fyodor , SHUKLA, Vipula K. , PETOLINO, Joseph F. , BAKER, Lisa W. , GARRISON, Robbi J. , BLUE, Ryan C. , MITCHELL, Jon C. , ARNOLD, Nicole L. , WORDEN, Sarah E.
IPC分类号： C12N15/62 , C07K14/00 , C12N15/82 , C12N15/09
CPC分类号： C07K14/4702 , C07K2319/81 , C12N15/8213 , C12N15/8243
摘要： Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.

5. 发明申请

WO2015167956A1 HAPLOID MAIZE TRANSFORMATION 审中-公开
标题翻译： HAPLOID MAIZE转换
公开(公告)号：WO2015167956A1
公开(公告)日：2015-11-05
申请号：PCT/US2015/027484
申请日：2015-04-24
申请人： DOW AGROSCIENCES LLC
发明人： PETOLINO, Joseph F. , STRANGE, Tonya L. , SAMUEL, Jayakumar Pon , BLUE, Ryan C. , SIMPSON, Matthew A.
IPC分类号： A01H5/00 , C12N15/82 , C12N15/87
CPC分类号： C12N15/8213
摘要： Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.
摘要翻译：公开了用位点特异性核酸酶转化雄激素衍生的单倍体细胞系的方法。在一些实施方案中，雄激素衍生的单倍体细胞系是来自玉米小孢子的植物组织培养物。此外，本公开提供了修饰，例如通过突变或靶向并将供体DNA整合到单倍体或双倍体组织基因组的特定基因座中的方法。本公开进一步提供了从单倍体或双倍体组织再生整个植物的方法，所述单体或双倍体组织含有特定基因组座位上的突变或整合在特定基因组基因组中的供体DNA，可从本发明公开内容获得。

6. 发明申请

WO2011049627A1 ENGINEERED ZINC FINGER PROTEINS TARGETING PLANT GENES INVOLVED IN FATTY ACID BIOSYNTHESIS 审中-公开
标题翻译：工程化锌指纹蛋白质涉及脂肪酸生物合成中涉及的植物基因
公开(公告)号：WO2011049627A1
公开(公告)日：2011-04-28
申请号：PCT/US2010/002817
申请日：2010-10-22
申请人： DOW AGROSCIENCES LLC , SANGAMO BIOSCIENCES, INC. , DEKELVER, Russell , GUPTA, Manju , MILLER, Jeffrey C. , NOVAK, Stephen , PETOLINO, Joseph F.
发明人： DEKELVER, Russell , GUPTA, Manju , MILLER, Jeffrey C. , NOVAK, Stephen , PETOLINO, Joseph F.
IPC分类号： C12N15/82 , C12N5/04 , A01H5/00
CPC分类号： C12N15/8218 , C07K14/415 , C12N9/22 , C12N15/8216 , C12N15/8247 , C12Y203/01041 , C12Y301/00
摘要： The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.
摘要翻译：本公开涉及靶向参与脂肪酸生物合成的植物中的基因的工程化锌指蛋白。还提供了使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。

7. 发明申请

WO2018093554A1 SITE SPECIFIC INTEGRATION OF A TRANSGNE USING INTRA-GENOMIC RECOMBINATION VIA A NON-HOMOLOGOUS END JOINING REPAIR PATHWAY 审中-公开
公开(公告)号：WO2018093554A1
公开(公告)日：2018-05-24
申请号：PCT/US2017/058980
申请日：2017-10-30
申请人： DOW AGROSCIENCES LLC
发明人： KUMAR, Sandeep , PETOLINO, Joseph F. , WORDEN, Andrew F. , BARONE, Pierluigi , SIMPSON, Matthew A. , STRANGE, Tonya L.
IPC分类号： A01H1/02 , A01H1/04 , A01H4/00 , A01H5/10 , C12N15/82
摘要： Compositions and methods to modify at least one target locus in a plant cell are provided, which comprises providing a plant cell, a plant, or a plant part with one or more target loci and one or more donor loci, providing at least one cleaving site specific nuclease to produce a double strand break within the target loci, followed by non-homologous end joining of at least one donor locus within at least one target locus. Target loci, donor loci and nuclease loci used in these methods, and plant cells, plants and plant parts comprising these target loci, donor loci, nuclease loci and/or the recombined loci are also provided.

8. 发明申请

WO2014165612A2 METHODS AND COMPOSITIONS FOR INTEGRATION OF AN EXOGENOUS SEQUENCE WITHIN THE GENOME OF PLANTS 审中-公开
标题翻译：在植物基因组中整合外源序列的方法和组合
公开(公告)号：WO2014165612A2
公开(公告)日：2014-10-09
申请号：PCT/US2014/032706
申请日：2014-04-02
申请人： DOW AGROSCIENCES LLC , SANGAMO BIOSCIENCES, INC.
发明人： AINLEY, W. Michael , GUSCHIN, Dmitry Y. , HAYDEN, Matthew , ISENEGGER, Daniel , MASON, John , MILLER, Jeffrey C. , PETOLINO, Joseph F. , RAN, Yidong , SAWBRIDGE, Tim , SPANGENBERG, German , WEBB, Steven R.
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C12N15/8274 , C12N15/8278 , C12N15/90 , C12N2800/80
摘要： Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.
摘要翻译：本文公开了用于在植物中平行或顺序转基因堆叠以产生具有选定表型的植物的方法和组合物。

9. 发明申请

WO2013169802A1 METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETED INTEGRATION OF TRANSGENES 审中-公开
标题翻译：用于核转移介导的靶向转移整合的方法和组合物
公开(公告)号：WO2013169802A1
公开(公告)日：2013-11-14
申请号：PCT/US2013/039979
申请日：2013-05-07
申请人： SANGAMO BIOSCIENCES, INC. , DOW AGROSCIENCES LLC
发明人： COST, Gregory, J. , URNOV, Fyodor , AINLEY, William M. , PETOLINO, Joseph F. , SAMUEL, Jayakumar Pon , WEBB, Steven R. , SASTRY-DENT, Lakshmi
IPC分类号： C12N15/87 , C12N15/00 , C12N5/10
CPC分类号： C12N15/8201 , C12N15/102 , C12N15/8209 , C12N15/8213 , C12N15/8216 , C12N15/8241 , C12N15/8247 , C12N15/8274 , C12N15/8278 , C12N15/8286 , C12N15/85 , C12N15/87 , C12N2800/80 , Y02A40/162
摘要： Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.
摘要翻译：本文公开了用于与供体分子同源性独立的靶向插入细胞基因组的方法和组合物。

10. 发明申请

WO2011091311A2 EXCISION OF TRANSGENES IN GENETICALLY MODIFIED ORGANISMS 审中-公开
标题翻译：转基因在经遗传修饰的生物体中的繁殖
公开(公告)号：WO2011091311A2
公开(公告)日：2011-07-28
申请号：PCT/US2011/022135
申请日：2011-01-21
申请人： DOW AGROSCIENCES LLC , RUSSELL, Sean , PETOLINO, Joseph F.
发明人： RUSSELL, Sean , PETOLINO, Joseph F.
IPC分类号： A01H5/00 , C12N15/82 , C12N15/29
CPC分类号： C12N15/8213 , A01H1/02 , C12N9/22 , C12N15/8241 , C12N15/8265
摘要： A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue specific promoter(s), e.g ., a pollen specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.
摘要翻译：
”用于删除植物中的DNA区域的方法。在一些实施方案中，所述方法包括用核酸分子转化植物，其中所述核酸分子编码与一种或多种组织特异性启动子可操作连接的一种或多种锌指核酸酶（ZFN）例如花粉特异性启动子。方法包括切除植物中的天然基因。因此，在一些实施方案中，工程化ZFN以识别侧接天然植物基因的序列。在进一步的实施方案中，ZFN在发育阶段特异性启动子的控制下表达，使得例如在发育的相对晚期阶段在植物中特异性切除核酸序列。包括可用于实施所公开的方法和由该方法产生的植物的核酸分子。

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