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1. 发明授权

US06617494B2 Methods for identifying transgenic plants using morphological markers 有权
公开(公告)号：US06617494B2
公开(公告)日：2003-09-09
申请号：US09832589
申请日：2001-04-11
申请人： Douglas C. Boyes , Carol M Hamilton , Andreas Kloti , Jorn Gorlach , Neil Hoffman
发明人： Douglas C. Boyes , Carol M Hamilton , Andreas Kloti , Jorn Gorlach , Neil Hoffman
IPC分类号： C12N1587
CPC分类号： A01H1/04 , C07K14/415 , C12N15/8209 , C12N15/8261 , Y02A40/146
摘要： Morphological markers are used in a method of visually identifying plants transformed with a nucleotide sequence (e.g., a heterologous gene). The nucleotide sequence is transformed into a plant that exhibits an abnormal phenotype for a morphological marker. If the transformation of the plant is successful, the progeny of the transformed plant will exhibit a normal phenotype. In a preferred embodiment, the plant is Arabidopsis and the morphological marker is Gl1, which is associated with trichome production on plant leaves. The method is also useful for identifying plants that are homozygous for the transformed gene, and for identifying transformants in the T2 generation that are true crosses, rather than self-crosses.

2. 发明授权

US06582900B1 Methods for the identification of modulators of magnesium chelatase expression or activity in plants 失效
标题翻译：鉴定镁螯合酶表达或植物活性调节剂的方法
公开(公告)号：US06582900B1
公开(公告)日：2003-06-24
申请号：US09659310
申请日：2000-09-12
申请人： Jeffrey P. Woessner , Adel Zayed , Jörn Görlach , Douglas C. Boyes , Keith R. Davis , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Kloti , Robert A. Ascenzi
发明人： Jeffrey P. Woessner , Adel Zayed , Jörn Görlach , Douglas C. Boyes , Keith R. Davis , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Kloti , Robert A. Ascenzi
IPC分类号： C12Q100
CPC分类号： C12Q1/25 , A01N61/00 , G01N2500/00
摘要： The present inventors have discovered that Mg-chelatase is essential for the growth of Arabidopsis. Specifically, the inhibition of Mg-chelatase CHL H gene expression in Arabidopsis seedlings results in varying levels of chlorosis (yellowing), significantly reduced growth and developmental abnormalities. Thus, Arabidopsis Mg-chelatase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that modulate Arabidopsis Mg-chelatase expression or activity, comprising: contacting a compound with a Arabidopsis Mg-chelatase, or a subunit thereof, and detecting the presence and/or absence of binding between said compound and said Mg-chelatase, or detecting a change in Mg-chelatase expression or activity. The methods of the invention are useful for the identification of herbicides and other compounds that can modulate plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of Mg-chelatase expression or function. Such compounds can be used to promote or manipulate plant growth and development.
摘要翻译：本发明人已经发现，Mg螯合酶对于拟南芥的生长是必需的。具体来说，拟南芥幼苗中Mg-螯合酶CHL H基因表达的抑制导致不同程度的褪绿（黄化），显着降低生长和发育异常。因此，拟南芥Mg螯合酶可用作鉴定除草剂的靶标。因此，本发明提供了鉴定调节拟南芥Mg-螯合酶表达或活性的化合物的方法，其包括：使化合物与拟南芥Mg-螯合酶或其亚单位接触，并检测其中是否存在结合所述化合物和所述Mg-螯合酶，或检测Mg螯合酶表达或活性的变化。本发明的方法可用于鉴定可以调节植物生长和发育的除草剂和其它化合物。此外，本发明的方法可用于鉴定刺激Mg-螯合酶表达或功能的表达或功能的化合物。这些化合物可用于促进或操纵植物的生长和发育。

3. 发明授权

US06800459B2 Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants 失效
标题翻译：调节草酸合酶和草酸转化酶表达或活性的方法和组合物
公开(公告)号：US06800459B2
公开(公告)日：2004-10-05
申请号：US10267763
申请日：2002-10-09
申请人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
发明人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
IPC分类号： C12N2106
CPC分类号： G01N33/68 , G01N2500/00
摘要： The present inventors have discovered that chorismate mutase and chorismate synthase are essential for plant growth. Specifically, the inhibition of chorismate mutase or chorismate synthase gene expression in plant seedlings results in severe chlorosis, reduced growth and developmental abnormalities. The inventors have proven that chorismate synthase and chorismate mutase can be used as targets for the identification of herbicides. Thus, the invention provides methods for the identification of chemicals that modulate chorismate synthase and chorismate mutase biochemical reactions. The methods of the invention are useful for the identification of herbicides and for the inhibition of plant growth and development.
摘要翻译：本发明人已经发现，草酸修饰酶和绒毛膜合成酶对于植物生长是必需的。具体来说，植物幼苗中的chorismate mutase或chorismate synthase gene表达的抑制导致严重的萎黄症，生长发育减退和发育异常。本发明人已经证明，可以使用草酸合成酶和氯酸盐变位酶作为鉴定除草剂的靶标。因此，本发明提供了用于鉴定调节绒毛膜合成酶和氯酸盐变位酶生化反应的化学物质的方法。本发明的方法可用于鉴定除草剂和用于抑制植物的生长和发育。

4. 发明授权

US06465217B1 Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants 失效
标题翻译：调节草酸合酶和草酸转化酶表达或活性的方法和组合物
公开(公告)号：US06465217B1
公开(公告)日：2002-10-15
申请号：US09610040
申请日：2000-07-05
申请人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
发明人： Douglas C. Boyes , Keith R. Davis , Jeffrey P. Woessner , Jörn Görlach , Carol M. Hamilton , Neil E. Hoffman , Andreas S. Klöti , Adel Zayed , Robert A. Ascenzi
IPC分类号： C12N2106
CPC分类号： G01N33/68 , G01N2500/00
摘要： The present inventors have discovered that chorismate mutase and chorismate synthase are essential for plant growth. Specifically, the inhibition of chorismate mutase or chorismate synthase gene expression in plant seedlings results in severe chlorosis, reduced growth and developmental abnormalities. Thus, in one aspect the invention provides compositions for the modulation of plant growth or development comprising chorismate synthase and chorismate mutase antisense and sense polynucleotides, dsRNA and ribozymes, and related expression cassettes and vectors. The compositions of the invention are particularly useful for the modulation and inhibition of plant growth. The invention further provides plants, plant cells, and seeds containing the polynucleotides of the invention. The inventors have proven that chorismate synthase and chorismate mutase can be used as targets for the identification of herbicides. Thus, the invention provides methods for the identification of chemicals that modulate chorismate synthase and chorismate mutase biochemical reactions. The methods of the invention are useful for the identification of herbicides and for the inhibition of plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of chorismate synthase or chorismate mutase expression or function. Such compounds can be used to promote plant growth and development.
摘要翻译：本发明人已经发现，草酸修饰酶和绒毛膜合成酶对于植物生长是必需的。具体来说，植物幼苗中的chorismate mutase或chorismate synthase gene表达的抑制导致严重的萎黄症，生长发育减退和发育异常。因此，在一个方面，本发明提供了用于调节植物生长或发育的组合物，其包含绒毛膜合成酶和辅助组氨酸变位酶反义和有义多核苷酸，dsRNA和核酶以及相关的表达盒和载体。本发明的组合物特别可用于调节和抑制植物生长。本发明还提供含有本发明的多核苷酸的植物，植物细胞和种子。本发明人已经证明，可以使用草酸合成酶和氯酸盐变位酶作为鉴定除草剂的靶标。因此，本发明提供了用于鉴定调节绒毛膜合成酶和氯酸盐变位酶生化反应的化学物质的方法。本发明的方法可用于鉴定除草剂和用于抑制植物的生长和发育。此外，本发明的方法可用于鉴定刺激基底膜合成酶或调节酸变位酶表达或功能的表达或功能的化合物。这些化合物可用于促进植物的生长和发育。

5. 发明授权

US06360482B1 Spray booth for reproducible application of agrichemicals 有权
标题翻译：喷涂室可重复使用农药
公开(公告)号：US06360482B1
公开(公告)日：2002-03-26
申请号：US09371105
申请日：1999-08-10
申请人： Douglas C. Boyes
发明人： Douglas C. Boyes
IPC分类号： A01G3100
CPC分类号： A01G9/247 , Y02A40/27
摘要： A spray booth is provided for reproducible application of agrichemicals to plants. The spray booth comprises a support member for supporting a plurality of plants, a cover having a plurality of sides and a top and forming an enclosure with the support member for enclosing the plants therein, an agrichemical source for providing a pressurized agrichemical, and a spray arm having a spray mechanism operably connected to the agrichemical source. Preferably, the spray arm is disposed within the enclosure and over the support member such that it extends across the width of the support member. The spray mechanism is used for spraying a predetermined amount of the agrichemical therefrom in the form of a fine mist along the length of the spray arm. Advantageously, at least one of the support member and the spray arm is laterally moveable relative to the other such that the plants disposed both lengthwise and widthwise on the support member are substantially uniformly misted with the agrichemical. In one embodiment of the present invention, the support member supporting the plants is stationary while the spray arm is adapted to move bidirectionally in a substantially horizontal plane thereabove. In an alternate embodiment, the spray arm is stationary and the support member supporting the plants is adapted to move bidirectionally in a substantially horizontal plane therebelow.
摘要翻译：提供了一个喷雾室，可以将植物的农药重复使用。喷雾室包括用于支撑多个植物的支撑构件，具有多个侧面和顶部并且形成具有用于将植物包围植物的支撑构件的壳体的盖子，用于提供加压农药的农艺源和喷雾器臂具有可操作地连接到农化源的喷雾机构。优选地，喷射臂设置在壳体内并且在支撑构件上方，使得其延伸跨过支撑构件的宽度。喷雾机构用于以喷雾臂的长度以细雾的形式喷洒预定量的农化材料。有利地，支撑构件和喷射臂中的至少一个相对于另一个可横向移动，使得在支撑构件上纵向和横向设置的植物基本上均匀地与农药混合。在本发明的一个实施例中，支撑植物的支撑构件是静止的，而喷射臂适于在上方基本水平的平面中双向移动。在替代实施例中，喷射臂是固定的，并且支撑植物的支撑构件适于在其下方的基本水平的平面中双向移动。

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