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1. 发明授权

US09260712B2 Surface display of whole antibodies in eukaryotes 有权
标题翻译：真核生物中全抗体的表面显示
公开(公告)号：US09260712B2
公开(公告)日：2016-02-16
申请号：US13251410
申请日：2011-10-03
申请人： Bianka Prinz , Natarajan Sethuraman , Dongxing Zha , Stefan Wildt , Piotr Bobrowicz
发明人： Bianka Prinz , Natarajan Sethuraman , Dongxing Zha , Stefan Wildt , Piotr Bobrowicz
IPC分类号： C12N15/10 , C07K16/28 , C07K16/32 , G01N33/68
CPC分类号： C12N15/1037 , C07K16/00 , C07K16/2887 , C07K16/32 , C07K2319/30 , C12N15/1034 , G01N33/6854
摘要： Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.
摘要翻译：描述了在真核生物宿主细胞（包括酵母和丝状真菌）表面上显示重组整个免疫球蛋白或免疫球蛋白文库的方法。该方法可用于筛选真核生物宿主细胞中重组免疫球蛋白的文库，以鉴定对感兴趣的抗原特异的免疫球蛋白。

2. 发明申请

US20110313137A1 HER2 ANTIBODY COMPOSITIONS 审中-公开
标题翻译： HER2抗体组合物
公开(公告)号：US20110313137A1
公开(公告)日：2011-12-22
申请号：US13203090
申请日：2010-02-24
申请人： Dongxing Zha
发明人： Dongxing Zha
IPC分类号： C07K16/40
CPC分类号： C07K16/32 , C07K2317/14 , C07K2317/41 , C07K2317/732 , C07K2317/92
摘要： The invention relates to compositions of Her2 antibody molecules with pre-selected N-linked glycosylation forms.
摘要翻译：本发明涉及具有预先选择的N-连接糖基化形式的Her2抗体分子的组合物。

3. 发明申请

US20100009866A1 Surface Display of Whole Antibodies in Eukaryotes 有权
标题翻译：真核生物中整个抗体的表面显示
公开(公告)号：US20100009866A1
公开(公告)日：2010-01-14
申请号：US12489900
申请日：2009-06-23
申请人： Bianka Prinz , Natarajan Sethuraman , Dongxing Zha , Stefan Wildt , Piotr Bobrowicz
发明人： Bianka Prinz , Natarajan Sethuraman , Dongxing Zha , Stefan Wildt , Piotr Bobrowicz
IPC分类号： C40B30/04 , C12N15/87 , G01N33/53
CPC分类号： C12N15/1037 , C07K16/00 , C07K16/2887 , C07K16/32 , C07K2319/30 , C12N15/1034 , G01N33/6854
摘要： Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.
摘要翻译：描述了在真核生物宿主细胞（包括酵母和丝状真菌）表面上显示重组整个免疫球蛋白或免疫球蛋白文库的方法。该方法可用于筛选真核生物宿主细胞中重组免疫球蛋白的文库，以鉴定对感兴趣的抗原特异的免疫球蛋白。

4. 发明申请

US20170101466A1 ANTI-BLYS ANTIBODIES 审中-公开
公开(公告)号：US20170101466A1
公开(公告)日：2017-04-13
申请号：US15315851
申请日：2015-06-01
申请人： Masahisa Handa , Robert Fick , Dongxing Zha
发明人： Masahisa Handa , Robert Fick , Dongxing Zha
IPC分类号： C07K16/24 , A61K39/395 , C12N15/81 , A61K45/06
CPC分类号： C07K16/241 , A61K31/192 , A61K31/196 , A61K31/405 , A61K31/407 , A61K31/4706 , A61K31/603 , A61K31/616 , A61K31/69 , A61K39/3955 , A61K45/06 , A61K2039/505 , C07K16/2875 , C07K2317/10 , C07K2317/14 , C07K2317/21 , C07K2317/33 , C07K2317/41 , C07K2317/52 , C07K2317/622 , C07K2317/76 , C07K2317/92 , C12N15/815 , A61K2300/00
摘要： The present invention provides antibodies and antigen-binding fragments thereof that bind specifically to BlyS, immunoglobulin chains thereof, variants thereof; and method of use thereof, e.g., for the treatment or prevention of inflammatory and/or immune diseases such as systemic lupus erythramatous; as well as polynucleotides encoding the immunoglobulin chains of such antibodies and fragments. Methods for the recombinant expression of immunoglobulin chains are also part of the present invention.

5. 发明授权

US09365846B2 Surface, anchored Fc-bait antibody display system 有权
标题翻译：表面，锚定的Fc诱饵抗体展示系统
公开(公告)号：US09365846B2
公开(公告)日：2016-06-14
申请号：US13990941
申请日：2011-11-29
申请人： Hussam Hisham Shaheen , Dongxing Zha
发明人： Hussam Hisham Shaheen , Dongxing Zha
IPC分类号： C40B40/10 , C12N15/10 , C07K16/00 , C07K16/32 , C07K16/40 , C07K16/46
CPC分类号： C12N15/1037 , C07K16/00 , C07K16/32 , C07K16/40 , C07K16/46 , C07K2317/14 , C07K2317/21 , C07K2317/35 , C07K2317/41 , C07K2317/52 , C40B40/10
摘要： The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. A bait complexed with a monovalent antibody fragment can be expressed on the surface of the host cell wherein the fragment may be assayed for antigen binding while full antibody is simultaneously secreted from the host cell. Methods of using the system for identifying antibodies that bind specifically to an antigen of interest are also provided. Polypeptides, polynucleotides and host cells useful for making the antibody display system are also provided along with methods of use thereof.
摘要翻译：本发明部分地提供同时使用分泌物和显示模式的抗体显示系统。与单价抗体片段复合的诱饵可以在宿主细胞的表面上表达，其中可以测定片段的抗原结合，同时从宿主细胞同时分泌全抗体。还提供了使用该系统鉴定特异性结合感兴趣的抗原的抗体的方法。还提供了可用于制备抗体展示系统的多肽，多核苷酸和宿主细胞及其使用方法。

6. 发明申请

US20140186334A1 SURFACE, ANCHORED FC-BAIT ANTIBODY DISPLAY SYSTEM 有权
标题翻译：表面，锚定的FC-BIT抗体显示系统
公开(公告)号：US20140186334A1
公开(公告)日：2014-07-03
申请号：US13990941
申请日：2011-11-29
申请人： Dongxing Zha , Hussam Hisham Shaheen
发明人： Dongxing Zha , Hussam Hisham Shaheen
IPC分类号： C12N15/10 , C07K16/46
CPC分类号： C12N15/1037 , C07K16/00 , C07K16/32 , C07K16/40 , C07K16/46 , C07K2317/14 , C07K2317/21 , C07K2317/35 , C07K2317/41 , C07K2317/52 , C40B40/10
摘要： The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. A bait complexed with a monovalent antibody fragment can be expressed on the surface of the host cell wherein the fragment may be assayed for antigen binding while full antibody is simultaneously secreted from the host cell. Methods of using the system for identifying antibodies that bind specifically to an antigen of interest are also provided. Polypeptides, polynucleotides and host cells useful for making the antibody display system are also provided along with methods of use thereof.
摘要翻译：本发明部分地提供同时使用分泌物和显示模式的抗体显示系统。与单价抗体片段复合的诱饵可以在宿主细胞的表面上表达，其中可以测定片段的抗原结合，同时从宿主细胞同时分泌全抗体。还提供了使用该系统鉴定特异性结合感兴趣的抗原的抗体的方法。还提供了可用于制备抗体展示系统的多肽，多核苷酸和宿主细胞及其使用方法。

7. 发明申请

US20140086916A1 METHOD FOR PREPARING Fc CONTAINING POLYPEPTIDES HAVING IMPROVED PROPERTIES 有权
标题翻译：用于制备具有改善性质的含Fc多聚体的方法
公开(公告)号：US20140086916A1
公开(公告)日：2014-03-27
申请号：US14117631
申请日：2012-05-22
申请人： Dongxing Zha
发明人： Dongxing Zha
IPC分类号： C07K16/28
CPC分类号： C07K16/2851 , C07K16/241 , C07K16/28 , C07K16/40 , C07K16/42 , C07K2317/41 , C07K2317/524 , C07K2317/71 , C07K2317/92
摘要： The present invention is directed to methods and compositions for the production of Fc-containing polypeptides comprising mutations at positions 243, 264, 267 and 328 of the Fc region.
摘要翻译：本发明涉及用于产生含Fc多肽的方法和组合物，其包含Fc区的243,264,267和328位的突变。

8. 发明授权

US08877686B2 Surface display of recombinant proteins in lower eukaryotes 有权
标题翻译：重组蛋白在低等真核生物中的表达
公开(公告)号：US08877686B2
公开(公告)日：2014-11-04
申请号：US12863911
申请日：2009-02-20
申请人： Dongxing Zha , Stefan Wildt
发明人： Dongxing Zha , Stefan Wildt
IPC分类号： C40B30/04 , C12N15/10 , C07K16/32 , C40B40/02 , C07K16/00 , C07K16/28 , C40B50/06 , C07K16/40
CPC分类号： C12N15/1037 , C07K16/005 , C07K16/2887 , C07K16/32 , C07K16/40 , C07K2317/51 , C07K2317/55 , C12N15/1055 , C40B40/02 , C40B40/08 , C40B50/06
摘要： Methods for display of recombinant proteins or protein libraries on the surface of lower eukaryotes such as yeast and filamentous fungi are described. The methods are useful for screening libraries of recombinant proteins in lower eukaryotes to identify particular proteins with desired properties from the array of proteins in the libraries. The methods are particularly useful for constructing and screening antibody libraries in lower eukaryotes.
摘要翻译：描述了在低等真核生物如酵母和丝状真菌的表面上显示重组蛋白或蛋白质文库的方法。该方法可用于筛选低等真核生物中的重组蛋白的文库，以从文库中的蛋白质阵列鉴定具有所需性质的特定蛋白质。该方法对于构建和筛选低等真核生物中的抗体文库特别有用。

9. 发明申请

US20120003695A1 METABOLIC ENGINEERING OF A GALACTOSE ASSIMILATION PATHWAY IN THE GLYCOENGINEERED YEAST PICHIA PASTORIS 审中-公开
标题翻译：葡萄糖生物化学烟草中糖基化途径的代谢工程
公开(公告)号：US20120003695A1
公开(公告)日：2012-01-05
申请号：US13202002
申请日：2010-02-24
申请人： Robert C. Davidson , Piotr Bobrowicz , Dongxing Zha
发明人： Robert C. Davidson , Piotr Bobrowicz , Dongxing Zha
IPC分类号： C12P21/00 , C12N9/64 , C12N15/81 , C12N9/16 , C12N9/24 , C12N1/19 , C12N9/72
CPC分类号： C12P21/005 , C07K16/32 , C07K2317/14 , C07K2317/41 , C12N1/16 , C12N9/1205 , C12N9/1241 , C12N9/90 , C12N15/815
摘要： Lower eukaryotic cells such as Pichia pastoris that normally cannot use galactose as a carbon source but which have been genetically engineered according to the methods herein to use galactose as a sole source of carbon are described. The cells are genetically engineered to express several of the enzymes comprising the Leloir pathway. In particular, the cells are genetically engineered to express a galactokinase, a UDP-galactose-C4-epimerase, and a galactose-1-phosphate uridyltransferase, and optionally a galactose permease. In addition, a method is provided for improving the yield of glycoproteins that have galactose-terminated or -containing N-glycans in cells that have been genetically engineered to produce glycoproteins with N-glycans having galactose residues but which normally lack the enzymes comprising the Leloir pathway comprising transforming the cells with one or more nucleic acid molecules encoding a galactokinase, a UDP-galactose-C4-epimerase, and a galactose-1-phosphate uridyltransferase. The methods and host cells described enable the presence or lack of the ability to assimilate galactose as a selection method for making recombinant cells. The methods and host cells are shown herein to be particularly useful for making immunoglobulins and the like that have galactose-terminated or containing N-glycans.
摘要翻译：描述了通常不能使用半乳糖作为碳源但已经根据本文的方法进行遗传工程化的低等真核细胞，例如巴斯德毕赤酵母，其使用半乳糖作为唯一的碳源。细胞被遗传工程化以表达几种包含Leloir途径的酶。特别地，细胞被遗传工程化以表达半乳糖激酶，UDP-半乳糖-C4差向异构酶和半乳糖-1-磷酸尿苷酸转移酶，以及任选的半乳糖通透酶。此外，提供了一种用于提高具有半乳糖封端或含有N-聚糖的糖蛋白的产率的方法，所述糖蛋白在已被遗传工程化以产生具有半乳糖残基的N-聚糖的糖蛋白的细胞中，但通常缺少包含Leloir的酶途径包括用编码半乳糖激酶，UDP-半乳糖-C4-差向异构酶和半乳糖-1-磷酸尿苷转移酶的一种或多种核酸分子转化细胞。所描述的方法和宿主细胞使得存在或缺乏同化半乳糖的能力作为制备重组细胞的选择方法。本文显示的方法和宿主细胞特别可用于制备具有半乳糖终止或含有N-聚糖的免疫球蛋白等。

10. 发明申请

US20100331192A1 SURFACE DISPLAY OF RECOMBINANT PROTEINS IN LOWER EUKARYOTES 有权
标题翻译：重组蛋白的表面显示在较低的毒素中
公开(公告)号：US20100331192A1
公开(公告)日：2010-12-30
申请号：US12863911
申请日：2009-02-20
申请人： Dongxing Zha , Stefan Wildt
发明人： Dongxing Zha , Stefan Wildt
IPC分类号： C40B10/00 , G01N33/53
CPC分类号： C12N15/1037 , C07K16/005 , C07K16/2887 , C07K16/32 , C07K16/40 , C07K2317/51 , C07K2317/55 , C12N15/1055 , C40B40/02 , C40B40/08 , C40B50/06
摘要： Methods for display of recombinant proteins or protein libraries on the surface of lower eukaryotes such as yeast and filamentous fungi are described. The methods are useful for screening libraries of recombinant proteins in lower eukaryotes to identify particular proteins with desired properties from the array of proteins in the libraries. The methods are particularly useful for constructing and screening antibody libraries in lower eukaryotes.
摘要翻译：描述了在低等真核生物如酵母和丝状真菌的表面上显示重组蛋白或蛋白质文库的方法。该方法可用于筛选低等真核生物中的重组蛋白的文库，以从文库中的蛋白质阵列鉴定具有所需性质的特定蛋白质。该方法对于构建和筛选低等真核生物中的抗体文库特别有用。

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