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1. 发明申请

US20050204432A1 Br2 and Dw3 promoters and methods of use 有权
标题翻译： Br2和Dw3启动子和使用方法
公开(公告)号：US20050204432A1
公开(公告)日：2005-09-15
申请号：US10931077
申请日：2004-08-31
申请人： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, the Dwarf3 (Dw3) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译：本发明涉及基因表达的调节，特别涉及用于调节植物中基因表达的方法和组合物。提供了包含与Brachytic2（Br2）启动子，Dw3（Dw3）启动子或其功能片段或变体可操作连接的目的多核苷酸分子的表达盒。另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。

2. 发明申请

US20070044180A1 Brachytic2 (Br2) promoter from maize and methods of use 失效
标题翻译：来自玉米的Brachytic2（Br2）启动子和使用方法
公开(公告)号：US20070044180A1
公开(公告)日：2007-02-22
申请号：US11589656
申请日：2006-10-30
申请人： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
IPC分类号： A01H5/00 , A01H1/00 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译：本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。

3. 发明授权

US07544857B2 Brachytic2 (Br2) promoter from maize and methods of use 失效
标题翻译：来自玉米的Brachytic2（Br2）启动子和使用方法
公开(公告)号：US07544857B2
公开(公告)日：2009-06-09
申请号：US11589656
申请日：2006-10-30
申请人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
IPC分类号： C12N15/82 , C07H21/04
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译：本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。

4. 发明授权

US07148401B2 Brachytic2 (BR2) promoter from maize and methods of use 有权
标题翻译：来自玉米的Brachytic2（BR2）启动子和使用方法
公开(公告)号：US07148401B2
公开(公告)日：2006-12-12
申请号：US10931077
申请日：2004-08-31
申请人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
IPC分类号： C12N15/82 , C07H21/04 , A01H5/00
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译：本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。

5. 发明申请

US20120329674A1 METHODS FOR LARGE SCALE FUNCTIONAL EVALUATION OF NUCLEOTIDE SEQUENCES IN PLANTS 审中-公开
标题翻译：植物核酸序列大规模功能评估方法
公开(公告)号：US20120329674A1
公开(公告)日：2012-12-27
申请号：US13312032
申请日：2011-12-06
申请人： Marc C. Albertsen , Susan J. Martino-Catt , Xiaomu Niu , Igor Cunha Oliveira , Dwight Tomes , Haiyin Wang , Deping Xu
发明人： Marc C. Albertsen , Susan J. Martino-Catt , Xiaomu Niu , Igor Cunha Oliveira , Dwight Tomes , Haiyin Wang , Deping Xu
IPC分类号： C40B30/06
CPC分类号： C12N15/1082
摘要： The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.
摘要翻译：本发明提供对植物中核苷酸序列的表达或功能的快速和大规模评估。本发明包括提供快速和大规模评估核苷酸序列的三个特定组分。第一组分包括在单一事件中递送不同工程化载体的文库或单个靶核苷酸序列的单一工程载体，其包含其功能期望在植物细胞中已知的序列。令人惊讶的是，申请人已经发现，将多个载体引入植物细胞主要导致单个转基因植物，其仅包含单个转化事件。本发明的第二个特征涉及高度可变形，快速循环和/或微型尺寸的植物，并且最终步骤涉及用于各种表型和质粒拯救的T0植物的大规模分析，以鉴定特定表型中存在的核苷酸序列。

6. 发明授权

US07928287B2 Methods for large scale functional evaluation of nucleotide sequences in plants 有权
标题翻译：植物核苷酸序列大规模功能评估方法
公开(公告)号：US07928287B2
公开(公告)日：2011-04-19
申请号：US10367417
申请日：2003-02-13
申请人： Dwight T. Tomes , Deping Xu , Haiyin Wang , Xiaomu Niu , Igor C. Oliveira
发明人： Dwight T. Tomes , Deping Xu , Haiyin Wang , Xiaomu Niu , Igor C. Oliveira
IPC分类号： A01H1/00 , A01H1/02
CPC分类号： C12N15/1082
摘要： The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.
摘要翻译：本发明提供对植物中核苷酸序列的表达或功能的快速和大规模评估。本发明包括提供快速和大规模评估核苷酸序列的三个特定组分。第一组分包括在单一事件中递送不同工程化载体的文库或单个靶核苷酸序列的单一工程载体，其包含其功能期望在植物细胞中已知的序列。令人惊讶的是，申请人已经发现，将多个载体引入植物细胞主要导致单个转基因植物，其仅包含单个转化事件。本发明的第二个特征涉及高度可变形，快速循环和/或微型尺寸的植物，并且最终步骤涉及用于各种表型和质粒拯救的T0植物的大规模分析，以鉴定特定表型中存在的核苷酸序列。

7. 发明申请

US20060037103A1 Cytokinin oxidase promoter from maize 失效
标题翻译：来自玉米的细胞分裂素氧化酶启动子
公开(公告)号：US20060037103A1
公开(公告)日：2006-02-16
申请号：US11074144
申请日：2005-03-07
申请人： Xiaomu Niu , Haiyin Wang , Dwight Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight Tomes , Sabine Hantke , Norbert Brugiere
IPC分类号： C12N15/82 , A01H1/00 , C12N15/87
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin. inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.
摘要翻译：本发明提供调节植物中核苷酸序列表达的组合物和方法。组合物可以包含用于具有组织特异性和/或细胞分裂素的启动子的新型核酸序列。诱导性。还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。其他方法提供植物中细胞分裂素氧化酶的下调。

8. 发明申请

US20120124697A1 PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING NUCLEOSIDE DIPHOSPHATASE KINASE (NDK) POLYPEPTIDES AND HOMOLOGS THEREOF 审中-公开
标题翻译：具有改变的根结构的植物，涉及编码核苷酸二磷酸酶激酶（NDK）多肽的基因的相关构建体和方法及其同源物
公开(公告)号：US20120124697A1
公开(公告)日：2012-05-17
申请号：US12671808
申请日：2008-08-29
申请人： Graziana Taramino , Scott V. Tingey , Stephen M. Allen , Dwight Tomes , Stanley Luck , Hajime Sakai , Xiaomu Niu
发明人： Graziana Taramino , Scott V. Tingey , Stephen M. Allen , Dwight Tomes , Stanley Luck , Hajime Sakai , Xiaomu Niu
IPC分类号： A01H5/00 , C12N15/82 , C12N15/54 , A01H5/10
CPC分类号： C12N15/8273 , C12N9/1229 , C12N15/8262
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译：分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。

9. 发明申请

US20070169226A1 Early Endosperm Promoter eep1 有权
标题翻译：早期胚乳启动子eep1
公开(公告)号：US20070169226A1
公开(公告)日：2007-07-19
申请号：US11621465
申请日：2007-01-09
申请人： Jeffrey Habben , Chris Zinselmeier , Dwight Tomes , Shane Abbitt , Timothy Helentjaris , Xiaomu Niu
发明人： Jeffrey Habben , Chris Zinselmeier , Dwight Tomes , Shane Abbitt , Timothy Helentjaris , Xiaomu Niu
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8234 , C12N9/0032 , C12N9/1085 , C12N15/8243 , C12N15/8261 , C12N15/8271 , C12N15/8295 , Y02A40/146
摘要： This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes, particularly in plant seeds and related female reproductive tissue. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for a seed-preferred promoter known as eep1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译：本发明一般涉及植物分子生物学领域。更具体地，本发明涉及用于时间和/或空间调节基因表达的方法和试剂，特别是在植物种子和相关的雌性生殖组织中。本发明还提供用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包含用于称为eep1的种子优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

10. 发明授权

US09090901B2 Plants with altered root architecture, related constructs and methods involving genes encoding leucine rich repeat kinase (LLRK) polypeptides and homologs thereof 有权
标题翻译：具有改变的根结构的植物，涉及编码富含亮氨酸重复激酶（LLRK）多肽的基因的相关构建体和方法及其同源物
公开(公告)号：US09090901B2
公开(公告)日：2015-07-28
申请号：US13347850
申请日：2012-01-11
申请人： Graziana Taramino , Stephen M. Allen , Stanley Luck , Xiaomu Niu , Hajime Sakai , Scott V. Tingey , Dwight Tomes
发明人： Graziana Taramino , Stephen M. Allen , Stanley Luck , Xiaomu Niu , Hajime Sakai , Scott V. Tingey , Dwight Tomes
IPC分类号： C12N15/63 , C12N15/82 , C07K14/415
CPC分类号： C12N15/8262 , C07K14/415 , C12N9/12 , C12N15/8201 , C12N15/8241 , C12Y207/11001
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译：分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。

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