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1. 发明申请

WO2012106723A1 POLYPHENOL PRODUCTION BY VACCINIUM MYRTILLUS CELL CULTURES 审中-公开
标题翻译：多环芳烃生产由真菌微生物细胞培养
公开(公告)号：WO2012106723A1
公开(公告)日：2012-08-09
申请号：PCT/US2012/024008
申请日：2012-02-06
申请人： DIANAPLANTSCIENCES, INC. , YOON, Sung-Yong , LALL, Sonia
发明人： YOON, Sung-Yong , LALL, Sonia
IPC分类号： A01N65/00 , A61K36/45
CPC分类号： C12N5/04 , A61K36/45
摘要： Cell cultures of Vaccinium myrtillus configured to grow in suspension culture in a liquid medium. The cells are derived from one or more V. myrtillus plant parts, such as an edible plant part (e.g., a leaf part or a berry part) or a stem part. The cells are adapted to grow to a high density is a relatively short period of time (e.g., about 7 days). In addition, the cells are adapted to produce high concentrations of polyphenols and/or procyanidins and essentially no anthocyanin. Methods for production of polyphenols and/or procyanidins from Vaccinium myrtillus cells grown in suspension culture are disclosed.
摘要翻译：酿酒酵母的细胞培养物配置为在液体培养基中悬浮培养中生长。细胞衍生自一种或多种V.myrtillus植物部分，例如可食用植物部分（例如叶部分或浆果部分）或茎部分。适于生长至高密度的细胞是相当短的时间段（例如约7天）。此外，细胞适于产生高浓度的多酚和/或原花青素，并且基本上不含花青素。公开了在悬浮培养物中生长的来自牛痘霉菌细胞的多酚和/或原花青素的生产方法。

2. 发明申请

WO2012047817A1 PRODUCTION AND EXTRACTION OF PROCYANIDINS FROM PLANT CELLS CULTURES 审中-公开
标题翻译：从植物细胞生产和提取方法文化
公开(公告)号：WO2012047817A1
公开(公告)日：2012-04-12
申请号：PCT/US2011/054648
申请日：2011-10-03
申请人： DIANAPLANTSCIENCES, INC. , YOON, Sung-Yong , PARK, Young, Chul , MCDONALD, Amy , DUBOIS, Camille, Pierre , LALL, Sonia
发明人： YOON, Sung-Yong , PARK, Young, Chul , MCDONALD, Amy , DUBOIS, Camille, Pierre , LALL, Sonia
IPC分类号： A23G1/04
CPC分类号： A23L1/3002 , A01H4/001 , A01H5/08 , A01H5/12 , A23L33/105 , A23L33/115 , A61K36/185 , C07D311/62 , C12N5/0025 , C12N5/04
摘要： A method of preparing cocoa oligomeric procyanidins from cocoa cell cultures grown in the presence of monosaccharide can increase production of procyanidins as follows: culturing cells sufficient to result in production of cocoa oligomeric procyanidins at a first rate; and introducing a monosaccharide to the cells sufficient for inducing the cells to produce the cocoa oligomeric procyanidins at a second rate that his higher than the first rate. The method can further include extracting the cocoa oligomeric procyanidins from the cells, and such extracting can occur between 1 day to 21 days after introduction of the monosaccharide. Optionally, the monosaccharide can be introduced in an amount about 0.5% to about to about 20% by volume of the culture medium. The monosaccharide can be glucose, sucrose, fructose, or the like. The monosaccharide can be introduced during or after a last phase of an exponential growth state.
摘要翻译：从单糖存在下生长的可可细胞培养物制备可可低聚原花青素的方法可以增加原花青素的产生，如下所述：培养足以产生可可低聚原花青素的细胞以一率; 以及将细胞引入足以诱导细胞以高于第一速率的第二速率产生可可低聚原花青素的细胞。该方法可以进一步包括从细胞中提取可可低聚原花青素，并且这种提取可以在引入单糖后1天至21天之间发生。任选地，单糖可以以培养基的体积的约0.5％至约20％的量引入。单糖可以是葡萄糖，蔗糖，果糖等。单糖可以在指数生长状态的最后阶段期间或之后引入。

3. 发明申请

WO2010114567A1 PRODUCTION AND EXTRACTION OF PROCYANIDINS FROM PLANT CELL CULTURES 审中-公开
标题翻译：生产和提取植物细胞培养物中的过程
公开(公告)号：WO2010114567A1
公开(公告)日：2010-10-07
申请号：PCT/US2009/042722
申请日：2009-05-04
申请人： DIANAPLANTSCIENCES, INC. , VENKATRAMESH, Mylavarapu , WAGNER, D., Ry , LALL, Sonia , LEJARD, Frederic
发明人： VENKATRAMESH, Mylavarapu , WAGNER, D., Ry , LALL, Sonia , LEJARD, Frederic
IPC分类号： C12N5/04
CPC分类号： A01H4/001
摘要： Provided herein are methods of making cocoa polyphenol preparations, which methods comprises harvesting cocoa polyphenols, for example, procyanidins, from the cell suspension culture. In examples of these methods, the resultant cocoa polyphenol preparation is substantially (or in some cases, completely) free of detectable caffeine and theobromine, and more generally substantially free of xanthine alkaloids. Methods of producing a cell suspension culture of cacao cells are also described, including cell suspension cultures useful for making cocoa polyphenol and, more specifically, procyanidin preparations. Theobroma sp and Herrania sp cell suspension cultures and cocoa polyphenol preparations made therefrom are also provided, in particular xanthine alkaloid-free (or caffeine- and/or theobromine-free) cocoa polyphenol preparations.
摘要翻译：本文提供制备可可多元酚制剂的方法，该方法包括从细胞悬浮培养物中收获可可多元酚，例如原花青素。在这些方法的实例中，所得到的可可多酚制剂基本上（或在一些情况下完全不含可检测的咖啡因和可可碱），更通常基本上不含黄嘌呤生物碱。还描述了产生可可细胞的细胞悬浮培养物的方法，包括可用于制备可可多元酚的细胞悬浮培养物，更具体地，原花青素制剂。还提供了自由基sp和赫拉尼亚芽孢杆菌细胞悬浮培养物和由其制备的可可多酚制剂，特别是不含黄嘌呤生物碱（或咖啡因和/或可可碱）的可可多酚制剂。

4. 发明公开

EP2414509A1 PRODUCTION AND EXTRACTION OF PROCYANIDINS FROM PLANT CELL CULTURES 有权
标题翻译：生产和原花青素的提取植物细胞培养
公开(公告)号：EP2414509A1
公开(公告)日：2012-02-08
申请号：EP09842834.5
申请日：2009-05-04
申请人： Dianaplantsciences, Inc.
发明人： VENKATRAMESH, Mylavarapu , WAGNER, D., Ry , LALL, Sonia , LEJARD, Frederic , YOON, Sung-Yong
IPC分类号： C12N5/04
CPC分类号： A01H4/001
摘要： Provided herein are methods of making cocoa polyphenol preparations, which methods comprises harvesting cocoa polyphenols, for example, procyanidins, from the cell suspension culture. In examples of these methods, the resultant cocoa polyphenol preparation is substantially (or in some cases, completely) free of detectable caffeine and theobromine, and more generally substantially free of xanthine alkaloids. Methods of producing a cell suspension culture of cacao cells are also described, including cell suspension cultures useful for making cocoa polyphenol and, more specifically, procyanidin preparations. Theobroma and Herrania sp cell suspension cultures and cocoa polyphenol preparations made therefrom are also provided, in particular xanthine alkaloid-free (or caffeine- and/or theobromine-free) cocoa polyphenol preparations.

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