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1. 发明授权

US06287568B1 Methods relating to immunogenic dextran-protein conjugates 失效
标题翻译：与免疫原性葡聚糖 - 蛋白质共轭物相关的方法
公开(公告)号：US06287568B1
公开(公告)日：2001-09-11
申请号：US09149997
申请日：1998-09-09
申请人： Denong Wang , Bernard F. Erlanger , Elvin A. Kabat
发明人： Denong Wang , Bernard F. Erlanger , Elvin A. Kabat
IPC分类号： A01N6500
CPC分类号： A61K47/646 , A61K47/61 , Y10S424/804 , Y10S424/812
摘要： This invention includes the conception of T-independent conjugate-vaccines and its application in the induction of antigen specific IgA response. We demonstrated that 1) &agr;(1,6)dextran can elicit a markedly enhanced IgA response in T-cell free mice (20-50 fold higher than in normal mice); 2)co-injection of the molecule with other antigens can enhance the IgA response to the co-antigen; and 3)a dextran-Gag conjugate can elicit the Gag-specific IgA. Thus, the invention identified &agr;(1,6)dextran as a carrier molecule for producing the T-independent conjugates and as an adjuvant for the enhancement of IgA production. The T-independent property of these conjugates makes it especially useful in vaccinations against HIV and other infectious and non-infectious diseases associated with T-cell deficiency.
摘要翻译：本发明包括T非依赖性缀合物疫苗的概念及其在诱导抗原特异性IgA应答中的应用。我们证明1）α（1,6）葡聚糖可以在无T细胞的小鼠中引起显着增强的IgA反应（比正常小鼠高20-50倍）; 2）共注射分子与其他抗原可以增强对共抗原的IgA反应; 和3）葡聚糖-Gag缀合物可以引发Gag特异性IgA。因此，本发明鉴定了α（1,6）葡聚糖作为产生T非依赖性缀合物的载体分子和用于增强IgA产生的佐剂。这些缀合物的T独立性质使其特别适用于针对HIV和与T细胞缺乏相关的其他感染性和非感染性疾病的疫苗接种。

2. 发明授权

US06177253B1 Screening methods for the identification of anti-HIV compounds employing a cyclosporine-specific monoclonal antibody that cross-reacts with HIV-1 p24 失效
标题翻译：使用与HIV-1 p24交叉反应的环孢菌素特异性单克隆抗体鉴定抗HIV化合物的筛选方法
公开(公告)号：US06177253B1
公开(公告)日：2001-01-23
申请号：US08894295
申请日：1998-01-08
申请人： Bernard F. Erlanger , Bi-Xing Chen
发明人： Bernard F. Erlanger , Bi-Xing Chen
IPC分类号： G01N3353
CPC分类号： G01N33/96 , A61K38/00 , A61K47/643 , C07K7/645 , C07K16/1054 , C07K16/14 , C07K16/4208 , C07K16/44 , G01N33/68 , G01N2410/08
摘要： The subject invention provides new uses for novel derivatives of cyclosporine A and antibodies directed thereto. Specifically, the derivatives and antibodies are useful in methods of treating AIDS, methods of purifying the Gag protein of HIV, methods of screening for anti-HIV compounds, and methods for detecting HIV in a subject. The derivatives and antibodies can also be incorporated into a kit useful for screening for anti-HIV compounds.
摘要翻译：本发明为环孢菌素A和针对其的抗体的新衍生物提供了新的用途。具体地，衍生物和抗体可用于治疗AIDS的方法，纯化HIV的Gag蛋白的方法，筛选抗HIV化合物的方法以及在受试者中检测HIV的方法。衍生物和抗体也可以并入用于筛选抗HIV化合物的试剂盒中。

3. 发明授权

US5925532A Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates 失效
标题翻译：通过使用针对探针标记和测定底物之间的反应产物的抗体来提高免疫测定程序的灵敏度
公开(公告)号：US5925532A
公开(公告)日：1999-07-20
申请号：US898583
申请日：1997-07-22
申请人： Bernard F. Erlanger , Bi-Xing Chen
发明人： Bernard F. Erlanger , Bi-Xing Chen
IPC分类号： C12Q1/70 , G01N33/53 , G01N33/537 , G01N33/542 , G01N33/543
CPC分类号： G01N33/54306 , C12Q1/6804
摘要： The subject invention provides an antibody which specifically binds to the product of a reaction between a labeling substance and a substrate. The subject invention also provides a method of making an immunogen used to produce the antibody of the subject invention. The invention further provides methods of using the subject antibody for detecting an antigen of interest in a sample, for example, detecting a protein comprising an amino acid sequence of interest and detecting a nucleic acid molecule comprising a nucleic acid sequence of interest, detecting a polypeptide such as those expressed by infectious agents, fungi or parasites.
摘要翻译：本发明提供了与标记物质和底物之间的反应的产物特异性结合的抗体。本发明还提供了制备用于产生本发明抗体的免疫原的方法。本发明还提供使用本发明抗体检测样品中目的抗原的方法，例如检测包含感兴趣的氨基酸序列的蛋白质并检测包含感兴趣的核酸序列的核酸分子，检测多肽例如由感染因子，真菌或寄生虫表达的那些。

4. 发明授权

US5650284A Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates 失效
标题翻译：通过使用针对探针标记和测定底物之间的反应产物的抗体来提高免疫测定程序的灵敏度
公开(公告)号：US5650284A
公开(公告)日：1997-07-22
申请号：US403649
申请日：1995-03-14
申请人： Bernard F. Erlanger , Bi-Xing Chen
发明人： Bernard F. Erlanger , Bi-Xing Chen
IPC分类号： G01N33/543 , C07K16/40 , C07K16/44 , C12N15/02 , C12P21/08 , C12Q1/68 , G01N33/58 , G01N33/53 , G01N33/537 , G01N33/542
CPC分类号： G01N33/581 , C07K16/40 , C07K16/44 , C12Q1/6804 , Y10S435/966
摘要： The subject invention provides an antibody which specifically binds to the product of a reaction between a labeling substance and a substrate. The subject invention also provides a method of making an immunogen used to produce the antibody of the subject invention. The invention further provides methods of using the subject antibody for detecting an antigen of interest in a sample, for example detecting a protein comprising an amino acid sequence of interest and detecting a nucleic acid molecule comprising a nucleic acid sequence of interest.
摘要翻译：本发明提供了与标记物质和底物之间的反应的产物特异性结合的抗体。本发明还提供了制备用于产生本发明抗体的免疫原的方法。本发明进一步提供使用本发明抗体检测样品中感兴趣的抗原的方法，例如检测包含感兴趣的氨基酸序列的蛋白质并检测包含感兴趣的核酸序列的核酸分子。

5. 发明授权

US06593137B1 Antibodies specific for fullerenes 失效
标题翻译：富勒烯特异性抗体
公开(公告)号：US06593137B1
公开(公告)日：2003-07-15
申请号：US09386658
申请日：1999-08-31
申请人： Bernard F. Erlanger , Bi-Xing Chen
发明人： Bernard F. Erlanger , Bi-Xing Chen
IPC分类号： C12N520
CPC分类号： B82Y30/00 , B82Y5/00 , C07K16/44 , G01N33/5308 , G01N2430/00 , Y10S530/807
摘要： This invention provides a hybridoma produced by the fusion of a mouse antibody-producing cell and a mouse myeloma which is designated 1-10F-8A and deposited with the ATCC under Accession Number PTA-279, said hybridoma producing a monoclonal antibody which binds to fullerene C60. This invention provides a mouse monoclonal antibody specific for a fullerene-C60 and produced by the mouse monoclonal antibody-producing hybridoma designated 1-10F-8A. The invention provides the amino acid and encoding nucleic acid sequences of the heavy and light chains of the 1-10F-8A monoclonal antibody. This invention also provides methods of determining a serum concentration of a fullerene in a subject and of purifying a fullerene from a sample.
摘要翻译：本发明提供了通过将小鼠抗体产生细胞与小鼠骨髓瘤融合而产生的杂交瘤，其被命名为1-10F-8A，并以保藏号PTA-279保藏在ATCC中，所述杂交瘤产生结合富勒烯的单克隆抗体 C60。本发明提供了对富勒烯-C60特异性并由指定为1-10F-8A的产生小鼠单克隆抗体的杂交瘤产生的小鼠单克隆抗体。本发明提供1-10F-8A单克隆抗体的重链和轻链的氨基酸和编码核酸序列。本发明还提供了确定受试者中富勒烯的血清浓度并从样品中纯化富勒烯的方法。

6. 发明授权

US5604092A Method for the detection of HIV-1 using a cyclosporine-specific monoclonal antibody that reacts with the P24 Gag protein 失效
标题翻译：使用与P24 Gag蛋白反应的环孢菌素特异性单克隆抗体检测HIV-1的方法
公开(公告)号：US5604092A
公开(公告)日：1997-02-18
申请号：US390133
申请日：1995-02-17
申请人： Bernard F. Erlanger , Bi-Xing Chen
发明人： Bernard F. Erlanger , Bi-Xing Chen
IPC分类号： A61K38/00 , A61K47/48 , C07K7/64 , C07K16/10 , C07K16/14 , C07K16/42 , C07K16/44 , C12P21/08 , G01N33/68 , G01N33/96 , C12Q1/70 , C07K16/00 , G01N33/53
CPC分类号： A61K47/48284 , C07K16/1054 , C07K16/14 , C07K16/4208 , C07K16/44 , C07K7/645 , G01N33/68 , G01N33/96 , A61K38/00 , G01N2410/08
摘要： Monoclonal antibodies to cyclosporine A (CsA), a potent immunosuppressant, were generated in Balb/c mice using a novel antigen prepared by linking CsA to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-CsA antibodies were generated, using CsA-BBa-bovine serum albumin (CsA-BBa-BSA) as the immunogen. Epitope mapping studies were performed using a series of singly substituted CsA derivatives and an antibody was identified that recognizes N-methyl leucine residues 9 and 10 of CsA. This antibody also cross-reacts with the HIV-1 Gag protein. A method for the detection of HIV-1 in patient samples employing this monoclonal antibody is disclosed.
摘要翻译：使用通过光活化交联剂4-苯甲酰基苯甲酸（BBa）将CsA与蛋白质载体连接而制备的新型抗原，在Balb / c小鼠中产生环孢菌素A（CsA）的单克隆抗体，其是有效的免疫抑制剂。使用CsA-BBa-牛血清白蛋白（CsA-BBa-BSA）作为免疫原，产生二十二种单克隆抗CsA抗体。使用一系列单取代的CsA衍生物进行表位作图研究，并鉴定出识别CsA的N-甲基亮氨酸残基9和10的抗体。该抗体也与HIV-1Gag蛋白交叉反应。公开了使用该单克隆抗体检测患者样品中HIV-1的方法。

7. 发明授权

US5350574A Derivatives of cyclosporina A, antibodies directed thereto and uses thereof 失效
标题翻译：环孢菌素A的衍生物，针对其的抗体及其用途
公开(公告)号：US5350574A
公开(公告)日：1994-09-27
申请号：US699468
申请日：1991-05-13
申请人： Bernard F. Erlanger , William L. Cleveland , Nicholas A. Cacalano
发明人： Bernard F. Erlanger , William L. Cleveland , Nicholas A. Cacalano
IPC分类号： G01N33/53 , A61K38/00 , A61K39/395 , A61K47/48 , A61P37/06 , C07K1/113 , C07K7/64 , C07K14/00 , C07K14/76 , C07K16/00 , C07K16/42 , C07K16/44 , C07K17/08 , C12P21/08 , G01N33/538 , G01N33/68 , G01N33/96 , A61K39/385 , A61K31/48 , A61K41/00 , C07K17/02 , C07K17/06
CPC分类号： A61K47/48284 , C07K16/4208 , C07K16/44 , C07K7/645 , G01N33/68 , G01N33/96 , A61K38/00 , G01N2410/08 , Y10S435/961 , Y10S435/964 , Y10S930/27 , Y10S930/28
摘要： This invention provides a molecule comprising cyclosporine A or a congener of cyclosporine A which is photochemically attached to a ligand containing a reactive group. This invention also provides a composition of matter which comprises a conjugate of a compound and the aforementioned molecule wherein the compound is bound to the molecule through the reactive group. This invention further provides an antibody directed to the aforementioned composition of matter specific for cyclosporine A or congener of cyclosporine A. This invention also provides methods for detecting the presence of cyclosporine A or congener thereof, methods for detecting the concentration of cyclosporine A or congener thereof, as well as a method of monitoring levels of cyclosporine A or congener of cyclosporine A in a subject. Finally, this invention provides a method of reducing the amount of endogenous immunoregulatory substances, a method of testing the potential of a pharmalogical agent as an immunoactive agent, as well as a method of testing a pharmalogical agent for immunosuppressive activity.
摘要翻译：本发明提供了包含环孢霉素A或环胞菌素A的同系物的分子，其与光化学连接至含有反应性基团的配体。本发明还提供了一种物质组合物，其包含化合物与上述分子的缀合物，其中化合物通过反应性基团与分子结合。本发明还提供针对上述环孢菌素A或环孢菌素A的同类物质特异性的组合物的抗体。本发明还提供了检测环孢菌素A或其同类物的存在的方法，用于检测环孢菌素A或其同类物的浓度的方法，以及监测受试者中环孢菌素A或环孢菌素A同类物的水平的方法。最后，本发明提供减少内源性免疫调节物质量的方法，测定作为免疫活性剂的药学试剂的潜力的方法，以及测试免疫抑制活性的药学试剂的方法。

8. 发明授权

US4895955A Non-radioactive carbodiimide precursor to nucleic acid probes 失效
标题翻译：核酸探针的非放射性碳二亚胺前体
公开(公告)号：US4895955A
公开(公告)日：1990-01-23
申请号：US199570
申请日：1988-05-27
申请人： John P. Ford , Charles W. Blewett , Michael D. Sublett , Bernard F. Erlanger , Alan F. Cook
发明人： John P. Ford , Charles W. Blewett , Michael D. Sublett , Bernard F. Erlanger , Alan F. Cook
IPC分类号： C07D495/04 , C07H21/00
CPC分类号： C07D495/04 , C07H21/00 , Y02P20/55
摘要： This invention relates to carbodiimide compounds of the formula:Z--L--W--N.dbd.C.dbd.N--R.sub.1wherein Z is a signalling moiety or a protecting group; L is a divalent linking group; W is an alkylene group having up to 24 carbon atoms which can be substituted or interrupted by a water solubility enhancing group or cleavable S--S group; and R.sub.1 is an alkyl group having up to 18 carbon atoms. These compounds are useful in the nonradioactive labelling of nucleic acid probes, and methods employing same.
摘要翻译：本发明涉及下式的碳二亚胺化合物：Z-L-W-N = C = N-R1其中Z是信号传导部分或保护基; L是二价连接基团; W是具有至多24个碳原子的亚烷基，其可以被水溶性增强基团或可切割的S-S基团取代或中断; 并且R 1是具有至多18个碳原子的烷基。这些化合物可用于核酸探针的非放射性标记和使用其的方法。

9. 发明授权

US5405785A Derivatives of cyclosporine A, antibodies directed thereto and uses thereof 失效
标题翻译：环孢菌素A的衍生物，针对其的抗体及其用途
公开(公告)号：US5405785A
公开(公告)日：1995-04-11
申请号：US6169
申请日：1993-01-15
申请人： Bernard F. Erlanger , William L. Cleveland , Nicholas A. Cacalano
发明人： Bernard F. Erlanger , William L. Cleveland , Nicholas A. Cacalano
IPC分类号： G01N33/53 , A61K38/00 , A61K39/395 , A61K47/48 , A61P37/06 , C07K1/113 , C07K7/64 , C07K14/00 , C07K14/76 , C07K16/00 , C07K16/42 , C07K16/44 , C07K17/08 , C12P21/08 , G01N33/538 , G01N33/68 , G01N33/96 , G01N33/545
CPC分类号： A61K47/48284 , C07K16/4208 , C07K16/44 , C07K7/645 , G01N33/68 , G01N33/96 , A61K38/00 , G01N2410/08 , Y10S435/961 , Y10S435/964 , Y10S930/27 , Y10S930/28
摘要： This invention provides a molecule comprising cyclosporine A or a congener of cyclosporine A which is photochemically attached to a ligand containing a reactive group. This invention also provides a composition of matter which comprises a conjugate of a compound and the aforementioned molecule wherein the compound is bound to the molecule through the reactive group. This invention further provides an antibody directed to the aforementioned composition of matter specific for cyclosporine A or congener of cyclosporine A. Finally, this invention provides a method of monitoring levels of cyclosporine A or congener of cyclosporine A in a subject.
摘要翻译：本发明提供了包含环孢霉素A或环胞菌素A的同系物的分子，其与光化学连接至含有反应性基团的配体。本发明还提供了一种物质组合物，其包含化合物与上述分子的缀合物，其中化合物通过反应性基团与分子结合。本发明还提供针对上述环孢菌素A或环孢菌素A的同类物质特异性的组合物的抗体。最后，本发明提供一种监测受试者中环孢菌素A或环孢菌素A同类物的水平的方法。

10. 发明授权

US5144010A Method of producing monoclonal auto-anti-idiotypic antibodies 失效
标题翻译：单克隆抗体独特型抗体的制备方法
公开(公告)号：US5144010A
公开(公告)日：1992-09-01
申请号：US273654
申请日：1989-11-18
申请人： Bernard F. Erlanger , William L. Cleveland
发明人： Bernard F. Erlanger , William L. Cleveland
IPC分类号： C07K16/42
CPC分类号： C07K16/4241 , Y10S530/809
摘要： This invention provides an auto-anti-idiotypic method for producing a monoclonal anti-idiotypic antibody. The method involves contacting lymphoid cells of an animal under suitable conditions with an effective antibody-raising amount of an antigen, collecting the lymphoid cells from the animal at a suitable time after the contacting and fusing the collected lymphoid cells with appropriate myeloma cells to produce a series of hybridoma cells each of which produces a monoclonal antibody. The method further involves screening, under suitable conditions, the series of hybridoma cells so produced to identify those which secrete a monoclonal antibody capable of binding to an antibody directed to the antigen, or to a receptor where the antigen is a ligand to the receptor, separately culturing a hybridoma cell so identified in an appropriate medium, and separately recovering under suitable conditions the monoclonal anti-idiotypic antibody produced by the hybridoma cell.
摘要翻译：本发明提供了用于产生单克隆抗独特型抗体的自身抗独特型方法。该方法包括在合适的条件下将动物的淋巴样细胞与有效的抗体提高量的抗原接触，并在收集的淋巴样细胞与合适的骨髓瘤细胞接合并融合后，在合适的时间从动物收集淋巴样细胞以产生一系列杂交瘤细胞各自产生单克隆抗体。该方法还包括在合适的条件下筛选如此产生的一系列杂交瘤细胞，以鉴定分泌能够结合针对抗原的抗体的单克隆抗体的那些，或者其中抗原是受体的配体的受体，分别培养在合适的培养基中鉴定的杂交瘤细胞，并在合适的条件下分别回收由杂交瘤细胞产生的单克隆抗独特型抗体。

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