专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06197523B1 Method for the detection, identification, enumeration and confirmation of circulating cancer and/or hematologic progenitor cells in whole blood 失效
标题翻译：全血中循环癌症和/或血液祖细胞的检测，鉴定，计数和确认方法
公开(公告)号：US06197523B1
公开(公告)日：2001-03-06
申请号：US08976886
申请日：1997-11-24
申请人： David L. Rimm , Robert A. Levine , Stephen C. Wardlaw , Paul Fiedler
发明人： David L. Rimm , Robert A. Levine , Stephen C. Wardlaw , Paul Fiedler
IPC分类号： G01N33574
CPC分类号： G01N15/042 , G01N33/56972 , G01N33/57484 , G01N2015/045 , Y10S436/813
摘要： A method for analyzing blood enables one to isolate, detect, enumerate and confirm under magnification the presence or absence of target cancer cells and/or hematologic progenitor cells which are known to circulate in blood. The analysis is performed in a sample of centrifuged anticoagulated whole blood. The analysis involves both morphometric and epitopic examination of the blood sample while the blood sample is disposed in a centrifuged blood sampling tube. The epitopic analysis of the presence or absence of cancer cells relies on the detection of epitopes which are known to present only on cancer cells; and the epitopic analysis of the presence or absence of hematologic progenitor cells relies on the detection of epitopes which are known to present only on hematologic progenitor cells. The targeted epitopes on the target cell types are epitopes which are also known to be absent on normal circulating blood cells; and the target cancer cell epitopes are epitopes which are known to be absent on target hematologic progenitor cells. Fluorophors with distinct emissions are coupled with antibodies which are directed against the targeted epitopes. The morphometric analysis is performed by staining the cells in the blood sample with an intracellular stain such as acridine orange which highlights the intracellular cell structure. Both the morphometric and epitopic analyses are preferably performed at or near the platelet layer of the expanded buffy coat in the centrifuged blood sample. The morphometric analysis and/or the epitopic analysis may be performed under magnification both visually and/or photometrically.
摘要翻译：用于分析血液的方法使得能够在放大下分离，检测，枚举和确认已知在血液中循环的靶癌细胞和/或血液祖细胞的存在或不存在。在离心的抗凝全血样品中进行分析。该分析涉及血液样品的形态测定和表征检查，同时将血液样品置于离心取样管中。癌细胞存在或不存在的代表性分析依赖于已知仅存在癌细胞的表位的检测; 并且血液祖细胞存在或不存在的表征分析依赖于已知仅存在血液祖细胞的表位的检测。目标细胞类型上的靶向表位是已知在正常循环血细胞上不存在的表位; 并且目标癌细胞表位是已知在靶血液祖细胞上不存在的表位。具有明显排放的荧光素与针对靶向表位的抗体相结合。通过用细胞内染色剂如吖啶橙染色血液样品中的细胞进行形态测定分析，突出细胞内细胞结构。形态测定和表征分析优选在离心血液样品中在扩张的血沉棕黄层的血小板层或其附近进行。形态测定分析和/或表征分析可以在视觉上和/或光度下放大进行。

2. 发明授权

US07129056B2 Method for the detection, identification, enumeration and confirmation of virally infected cells and other epitopically defined cells in whole blood 失效
公开(公告)号：US07129056B2
公开(公告)日：2006-10-31
申请号：US11155856
申请日：2005-06-20
申请人： David L. Rimm , Paul Fiedler , Robert A. Levine , Stephen C. Wardlaw
发明人： David L. Rimm , Paul Fiedler , Robert A. Levine , Stephen C. Wardlaw
IPC分类号： G01N33/53
CPC分类号： G01N33/56972 , C07K16/109 , G01N15/042 , G01N33/57484 , G01N33/6878 , G01N2015/045 , Y10S436/81
摘要： A method for analyzing blood enables one to isolate, detect, enumerate and confirm under magnification the presence of target cells which have expressed surface epitopes that indicate intracellular infection by various viruses or other infectious agents, and also cells which have expressed surface epitopes that indicate the presence of non-infectious medical conditions. The analysis involves the examination of cells in the blood sample for the presence or absence of particular surface epitopes while the blood sample is disposed in a centrifuged blood sampling container. The epitopic analysis for the presence or absence of infected cells, or cells which indicate the presence of non-infectious medical conditions relies on the detection of known target expressed epitopes. The target epitopes on the target cell types are epitopes which are also known to be absent on normal circulating cells in the blood. Fluorophores or other labels with distinct wavelength emissions are coupled with specific binding agents such as lectins, antibodies, aptamers, or the like, which are directed against the target expressed epitopes. The epitopic analyses may be performed in or near the expanded buffy coat layer in the centrifuged blood sample. The epitopic analysis may be performed under magnification either visually and/or photometrically. The blood sampling container is sized to hold between about 1 and about 20 ml, preferably about 10 ml of blood, and contains an insert that occupies about 90–98% of the volume of the container bore in the area of the container where the target cells will, if present, be detected. The insert forces the target cells in question to reside in an annular space in the container which is adjacent to the circumference of the container bore. The entire analysis can be performed in a relatively short period of time which is typically a matter of minutes to single digit hours.

3. 发明授权

US06911315B2 Method for the detection, identification, enumeration and confirmation of virally infected cells and other epitopically defined cells in whole blood 失效
公开(公告)号：US06911315B2
公开(公告)日：2005-06-28
申请号：US10042016
申请日：2002-01-10
申请人： David L. Rimm , Paul Fiedler , Robert A. Levine , Stephen C. Wardlaw
发明人： David L. Rimm , Paul Fiedler , Robert A. Levine , Stephen C. Wardlaw
IPC分类号： C07K16/10 , C12Q1/70 , G01N15/04 , G01N33/569 , G01N33/574 , G01N33/68 , G01N33/53
CPC分类号： G01N33/56972 , C07K16/109 , G01N15/042 , G01N33/57484 , G01N33/6878 , G01N2015/045 , Y10S436/81
摘要： A method for analyzing blood enables one to isolate, detect, enumerate and confirm under magnification the presence of target cells which have expressed surface epitopes that indicate intracellular infection by various viruses or other infectious agents, and also cells which have expressed surface epitopes that indicate the presence of non-infectious medical conditions. The analysis involves the examination of cells in the blood sample for the presence or absence of particular surface epitopes while the blood sample is disposed in a centrifuged blood sampling container. The epitopic analysis for the presence or absence of infected cells, or cells which indicate the presence of non-infectious medical conditions relies on the detection of known target expressed epitopes. The target epitopes on the target cell types are epitopes which are also known to be absent on normal circulating cells in the blood. Fluorophores or other labels with distinct wavelength emissions are coupled with specific binding agents such as lectins, antibodies, aptamers, or the like, which are directed against the target expressed epitopes. The epitopic analyses may be performed in or near the expanded buffy coat layer in the centrifuged blood sample. The epitopic analysis may be performed under magnification either visually and/or photometrically. The blood sampling container is sized to hold between about 1 and about 20 ml, preferably about 10 ml of blood, and contains an insert that occupies about 90-98% of the volume of the container bore in the area of the container where the target cells will, if present, be detected. The insert forces the target cells in question to reside in an annular space in the container which is adjacent to the circumference of the container bore. The entire analysis can be performed in a relatively short period of time which is typically a matter of minutes to single digit hours.

4. 发明授权

US06670197B2 Method for assaying whole blood for the presence or absence of circulating cancer or other target cell fragments 失效
标题翻译：用于测定全血存在或不存在循环癌或其它靶细胞碎片的方法
公开(公告)号：US06670197B2
公开(公告)日：2003-12-30
申请号：US09800344
申请日：2001-03-05
申请人： David L. Rimm , Stephen C. Wardlaw , Robert A. Levine , Paul Fiedler
发明人： David L. Rimm , Stephen C. Wardlaw , Robert A. Levine , Paul Fiedler
IPC分类号： G01N33543
CPC分类号： G01N15/042 , G01N33/56972 , G01N33/57484 , G01N2015/045
摘要： This method for analyzing blood enables one to isolate, detect, enumerate and confirm under magnification the presence or absence of fragments of target analyte cancer cells which are circulating in blood. The analysis is performed in a sample of centrifuged anticoagulated whole blood. The analysis of the presence or absence of fragments of cancer cells relies on the detection of external or internal binding sites which are known to be present only in or on tumorous cancer cells. Fluorophors with distinct wavelength emissions are coupled with antibodies, or other binding moieties such as complementary nucleotide sequences, which antibodies are directed against the epithelial cell fragment membrane binding sites, such as internal or external surface epitopes on the cell fragments, or internal binding sites on cell organelles; and which nucleotide sequences are complementary to portions of cell fragment RNA and/or DNA. The labled binding agents are humoric or soluble in the blood sample. The labeled fluorometric binding site-specific materials may be coupled to small plastic beads which have a density or specific gravity that is preferably greater than the specific gravity or density of the red blood cells. The target cell fragments are less dense than the red cells, and typically have the same density or specific gravity as the platelets or white blood cells in the blood sample. Any of the labeled beads which couple with target cell analyte fragments will have a density or specific gravity that is less than the red cells in the blood sample. Thus cell fragment/labeled bead couples will gravitate into an area in the centrifuged blood sample which area is somewhere above the centrifuged red cell layer. The detection of the labeled target analyte/particle couples can be performed in situ in the centrifuged blood sample either visually or photometrically.
摘要翻译：用于分析血液的方法使得能够在放大下分离，检测，枚举和确认在血液中循环的靶分析物癌细胞的片段的存在或不存在。在离心的抗凝全血样品中进行分析。对癌细胞片段的存在或不存在的分析依赖于已知仅存在于肿瘤细胞中或肿瘤细胞中的外部或内部结合位点的检测。具有不同波长发射的荧光体与抗体或其它结合部分例如互补核苷酸序列偶联，所述抗体针对上皮细胞片段膜结合位点，例如细胞片段上的内部或外部表面表位或内部结合位点细胞器哪些核苷酸序列与细胞片段RNA和/或DNA的部分互补。标记的粘合剂是幽默的或可溶于血液样品。标记的荧光结合位点特异性材料可以耦合到具有优选大于红细胞的比重或密度的密度或比重的小塑料珠粒。靶细胞碎片比红细胞密度低，通常具有与血液样品中的血小板或白细胞相同的密度或比重。与靶细胞分析物片段偶联的任何标记珠粒将具有小于血液样品中红细胞的密度或比重。因此，细胞碎片/标记的珠子对将被引入离心的血液样品中的该区域位于离心红细胞层上方的区域中。标记的目标分析物/颗粒对的检测可以在离心的血液样品中在视觉上或光度下原位进行。

5. 发明授权

US06444436B1 Evacuated container assembly for analysis of a blood sample for the presence or absence of rare events 失效
标题翻译：抽真空的容器组件，用于分析血液样本是否存在罕见事件
公开(公告)号：US06444436B1
公开(公告)日：2002-09-03
申请号：US09507635
申请日：2000-02-22
申请人： David L. Rimm , Paul Fiedler , Robert A. Levine , Stephen C. Wardlaw
发明人： David L. Rimm , Paul Fiedler , Robert A. Levine , Stephen C. Wardlaw
IPC分类号： G01N33574
CPC分类号： G01N33/57488 , Y10S436/81
摘要： A method for analyzing blood enables one to isolate, detect, enumerate and confirm under magnification the presence or absence of target cancer cells and/or hematologic progenitor cells, or other rare events which are known to circulate in blood. The analysis is performed in a sample of centrifuged anticoagulated whole blood. The analysis involves both morphometric and epitopic examination of the blood sample while the blood sample is disposed in a centrifuged blood sampling container. The epitopic analysis of the presence or absence of cancer cells relies on the detection of epitopes which are known to be present on cancer cells and not on normal circulating blood cells; and the epitopic analysis of the presence or absence of hematologic progenitor cells relies on the detection of epitopes which are known to be present on hematologic progenitor cells and not on normal circulating blood cells. The targeted epitopes on the target cell types are epitopes which are also known to be absent on normal circulating blood cells; and the target cancer cell epitopes are epitopes which are known to be absent on target hematologic progenitor cells. Fluorophors with distinct emissions are coupled with antibodies which are directed against the targeted epitopes. The morphometric analysis is performed by staining the cells in the blood sample with an intracellular stain, such as acridine orange, which highlights the intracellular cell structure. Both the morphometric and epitopic analyses are preferably performed in the expanded buffy coat layer in the centrifuged blood sample. The morphometric analysis and/or the epitopic analysis may be performed under magnification both visually and/or photometrically.
摘要翻译：用于分析血液的方法使得能够在放大下分离，检测，枚举和确认靶癌细胞和/或血液祖细胞的存在或不存在，或已知在血液中循环的其它罕见事件。在离心的抗凝全血样品中进行分析。该分析涉及血液样品的形态测定和表征检查，同时将血液样品置于离心取样容器中。癌细胞存在或不存在的表位分析依赖于已知存在于癌细胞而不是正常循环血细胞上的表位的检测; 并且血液祖细胞存在或不存在的表征分析依赖于已知存在于血液祖细胞而不是正常循环血细胞上的表位的检测。目标细胞类型上的靶向表位是已知在正常循环血细胞上不存在的表位; 并且目标癌细胞表位是已知在靶血液祖细胞上不存在的表位。具有明显排放的荧光素与针对靶向表位的抗体相结合。通过用细胞内染色剂如吖啶橙染色血液样品中的细胞进行形态测定分析，其突出显示细胞内细胞结构。形态测定和表征分析优选在离心血液样品中的膨胀的血沉棕黄层中进行。形态测定分析和/或表征分析可以在视觉上和/或光度下放大进行。

6. 发明授权

US5496704A Method for in vitro detection of formed elements in biological samples 失效
标题翻译：体外检测生物样品中形成元素的方法
公开(公告)号：US5496704A
公开(公告)日：1996-03-05
申请号：US312513
申请日：1994-09-26
申请人： Paul Fiedler , Robert A. Levine , Stephen C. Wardlaw
发明人： Paul Fiedler , Robert A. Levine , Stephen C. Wardlaw
IPC分类号： G01N33/569 , G01N33/574
CPC分类号： G01N33/569 , G01N33/56966 , G01N33/574 , Y10S436/80 , Y10S436/805 , Y10S436/81 , Y10S436/813 , Y10S436/824
摘要： A biological sample such as feces, sputum, cervical tissue, pleural fluids, exudates, cytologic specimens, or the like, is tested for the presence or absence of: ova; parasites; microorganisms; inflammatory, neoplastic tissue cells; or other target materials which are indicative of infestation, disease or infection. The sample is mixed with a buffer fluid and placed in a transparent tube which contains a volume-constricting cylindrical insert for gravimetric separation of components of the sample. The mixture is centrifuged, and the annular space between the insert and tube bore is examined under magnification for the presence of the target materials.
摘要翻译：对生物样品如粪便，痰液，宫颈组织，胸膜液，渗出物，细胞学标本等进行测试，以证明是否存在：ova; 寄生虫微生物炎性，肿瘤组织细胞; 或指示感染，疾病或感染的其他目标材料。将样品与缓冲液混合并置于含有体积收缩的圆柱形插入物的透明管中，用于重量分离样品组分。将混合物离心，并且在放大下检查插入件和管孔之间的环形空间以存在目标材料。

7. 发明授权

US09873118B2 Biologic fluid analysis cartridge with sample handling portion and analysis chamber portion 有权
公开(公告)号：US09873118B2
公开(公告)日：2018-01-23
申请号：US13341618
申请日：2011-12-30
申请人： John A. Verrant , Niten V. Lalpuria , Igor Nikonorov , Darryn W. Unfricht , Benjamin Ports , Stephen C. Wardlaw , Robert A. Levine , Robert Holt , Kyle Hukari
发明人： John A. Verrant , Niten V. Lalpuria , Igor Nikonorov , Darryn W. Unfricht , Benjamin Ports , Stephen C. Wardlaw , Robert A. Levine , Robert Holt , Kyle Hukari
IPC分类号： A61J1/06 , B01L3/00
CPC分类号： B01L3/502707 , B01L3/5027 , B01L3/50273 , B01L2200/027 , B01L2300/0861
摘要： A biological fluid analysis cartridge is provided. In certain embodiments, the cartridge includes a base plate extending between a sample handling portion and an analysis chamber portion. A handling upper panel is attached to the base plate within the sample handling portion. A collection port is at least partially formed with the handling upper panel. An initial channel and a secondary channel are formed between the handling upper panel and the base plate. The collection port and initial and secondary channels are in fluid communication with one another. A chamber upper panel is attached to the base plate within the analysis chamber portion. At least one analysis chamber is formed between the chamber upper panel and the base plate. The secondary channel and the analysis chamber are in fluid communication with one another.

8. 发明申请

US20160320369A1 METHOD AND APPARATUS FOR PERFORMING HEMATOLOGIC ANALYSIS USING AN ARRAY-IMAGING SYSTEM FOR IMAGING AND ANALYSIS OF A CENTRIFUGED ANALYSIS TUBE 审中-公开
标题翻译：使用阵列成像系统进行血液分析的方法和装置，用于成像分析和分析分析管
公开(公告)号：US20160320369A1
公开(公告)日：2016-11-03
申请号：US15207074
申请日：2016-07-11
申请人： Joshua D. Levine , Robert A. Levine , Stephen C. Wardlaw , Craig Stout , David A. Clipper
发明人： Joshua D. Levine , Robert A. Levine , Stephen C. Wardlaw , Craig Stout , David A. Clipper
IPC分类号： G01N33/49 , G06T7/00 , B01L3/00 , B01L9/06 , G01N15/04 , G01N15/05
CPC分类号： G01N33/491 , B01L3/50215 , B01L9/065 , B01L2300/027 , B01L2300/0609 , B01L2300/0654 , B01L2300/0838 , G01N15/042 , G01N15/05 , G01N2015/045 , G01N2800/22 , G06T7/0012 , G06T2207/30024
摘要： A method and device for analyzing a hematologic sample centrifuged within a capillary tube is provided. The device includes a tube holder, a sample imaging device, a processor, and a sample data display. The sample imaging device is operable to create a digital image of the sample within a region of the tube. The region is defined by substantially all of the radial width and axial length of the sample residing within the internal cavity of the tube in the region where the float resides after centrifugation. The sample imaging device is operable to produce signals representative of the image. The processor is adapted to produce information relating to bands of interest within the image based on the signals from the sample imaging device. The sample data display is adapted to display the results therefrom and/or a digital image of the sample within the region.
摘要翻译：提供了用于分析在毛细管内离心的血液样品的方法和装置。该装置包括管座，样品成像装置，处理器和样品数据显示器。样品成像装置可操作以在管的区域内产生样品的数字图像。该区域由离心后浮子所在的区域中位于管内腔中的样品的基本上所有的径向宽度和轴向长度限定。样品成像装置可操作以产生表示图像的信号。处理器适于基于来自样本成像装置的信号产生与图像内的感兴趣的频带相关的信息。样本数据显示器适于在该区域内显示样本的结果和/或数字图像。

9. 发明授权

US08774487B2 Method and apparatus for remotely performing hematologic analysis utilizing a transmitted image of a centrifuged analysis tube 有权
标题翻译：利用离心分析管的透射图像远程执行血液分析的方法和装置
公开(公告)号：US08774487B2
公开(公告)日：2014-07-08
申请号：US13016392
申请日：2011-01-28
申请人： Joshua D. Levine , Robert A. Levine , Stephen C. Wardlaw , Craig Stout , David A. Clipper
发明人： Joshua D. Levine , Robert A. Levine , Stephen C. Wardlaw , Craig Stout , David A. Clipper
IPC分类号： G06K9/00
CPC分类号： G01N33/491 , B01L3/50215 , B01L9/065 , B01L2300/027 , B01L2300/0609 , B01L2300/0654 , B01L2300/0838 , G01N15/042 , G01N15/05 , G01N2015/045 , G01N2800/22 , G06T7/0012 , G06T2207/30024
摘要： An apparatus for and method of analyzing hematologic samples deposited within a capillary tube is provided. The method includes the steps of: a) imaging a region of sample centrifuged within a capillary tube using a first analysis device, which region is defined by substantially all of the radial width and axial length of the sample residing within the internal cavity of the tube where the float resides after centrifugation, and producing signals representative of the image; b) communicating the signals representative of the image to a second analysis device independent of, and remotely located from, the first analysis device; c) processing the signals representative of the image using the second analysis device and producing analysis data based on the signals; and d) displaying the image of the region of the sample using the second analysis device.
摘要翻译：提供了一种用于分析沉积在毛细管内的血液样品的方法和方法。该方法包括以下步骤：a）使用第一分析装置对在毛细管内离心的样品的区域进行成像，该区域由位于管内腔内的样品的基本上所有的径向宽度和轴向长度限定离心后浮子位于其中，并产生代表图像的信号; b）将表示所述图像的信号传送到与所述第一分析装置无关并且远离所述第一分析装置的第二分析装置; c）使用第二分析装置处理表示图像的信号，并基于该信号产生分析数据; 以及d）使用所述第二分析装置显示所述样品的区域的图像。

10. 发明申请

US20130029373A1 METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING 有权
标题翻译：使用荧光猝灭和/或漂白分析个体细胞或颗粒物的方法和装置
公开(公告)号：US20130029373A1
公开(公告)日：2013-01-31
申请号：US13645075
申请日：2012-10-04
申请人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
发明人： Robert A. Levine , Stephen C. Wardlaw , Niten V. Lalpuria , Darryn W. Unfricht
IPC分类号： G01N21/64 , C12M1/34
CPC分类号： G01N33/49 , G01N15/1463 , G01N15/1475 , G01N21/6428 , G01N21/6456 , G01N33/48 , G01N33/5091 , G01N2015/008 , G01N2015/1477 , G01N2021/6432 , G01N2021/6439 , G01N2201/062
摘要： A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values.
摘要翻译：提供了一种用于分析血液样本的方法，其包括以下步骤：提供具有每个第一和第二成分中的一个或多个的血液样品; 将着色剂与样品混合，该着色剂可操作以使第一组分和第二组分发光和吸收光; 照亮样品的至少一部分; e）对样品的一部分进行成像; 确定每个第一组分和第二组分的荧光值; 确定每个第一组分和第二组分的光密度值; 以及使用所确定的荧光和光密度值来识别第一成分和第二成分。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式