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1. 发明授权

US5707800A Retinoic acid response elements and assays employing same 失效
标题翻译：视黄酸反应元件和采用其的测定法
公开(公告)号：US5707800A
公开(公告)日：1998-01-13
申请号：US346342
申请日：1994-11-28
申请人： David John Mangelsdorf , Ronald M. Evans , Kazuhiko Umesono , Steven A. Kliewer
发明人： David John Mangelsdorf , Ronald M. Evans , Kazuhiko Umesono , Steven A. Kliewer
IPC分类号： C12N5/10 , C07K14/705 , C12N15/09 , C12N15/85 , C12P21/02 , C12Q1/02 , C12Q1/34 , C12Q1/48 , C12Q1/66 , C12Q1/68 , C12R1/91 , G01N33/50 , C07H17/00 , C12N15/00 , C12P21/06
CPC分类号： C07K14/70567 , C12N15/85 , G01N33/5008 , C12N2830/008 , C12N2830/85 , G01N2500/10
摘要： DNA segments have been discovered, and characterized by sequence, which are response elements operative to confer responsiveness to retinoic acid, or derivatives thereof, for the transcriptional activation of promoters in cells. By using transcriptional control regions comprising response elements of the present invention in combination with a functional promoter, it is now possible to provide recombinant DNA vectors containing a gene, the transcription (and, thereby, also expression) of which is under the control of a promoter, the transcriptional activity of which is responsive to (and increased by) retinoic acid or derivatives thereof.
摘要翻译：已经发现了DNA区段，并且其特征在于序列，其是可操作地赋予视黄酸或其衍生物对于细胞中启动子的转录激活的响应的响应元件。通过使用包含本发明的响应元件与功能启动子组合的转录控制区，现在可以提供含有基因的重组DNA载体，其转录（并且由此也由其表达）处于一个启动子，其转录活性对视黄酸或其衍生物有反应（并增加）。

2. 发明授权

US06893830B1 Method of screening oxysterol activation of LXRα 有权
标题翻译：氧化固醇激活LXRα的方法
公开(公告)号：US06893830B1
公开(公告)日：2005-05-17
申请号：US09603131
申请日：2000-06-23
申请人： Bethany A. Janowski , David John Mangelsdorf
发明人： Bethany A. Janowski , David John Mangelsdorf
IPC分类号： G01N33/53 , G01N33/566 , G01N33/74 , G01N33/92
CPC分类号： G01N33/566 , G01N33/74 , G01N33/92
摘要： The present invention provides a method of screening for agonists of an oxycholesterol activator of LXRα transcription, comprising the steps of: introducing a reporter construct and an LXR expression construct into a host cell; treating the host cell with potential LXR-specific ligands; and identifying compounds which activate LXRα transcription. Also provided is a method of screening for antagonists of an oxycholesterol activator of LXRα transcription, comprising the steps of: introducing a reporter construct and an LXR expression construct into a host cell; pretreating the host cell with activators of LXRα transcription; contacting the host cell with potential antagonists of LXRα transcription; and identifying compounds which block the activation the LXRα transcription.
摘要翻译：本发明提供了筛选LXRα转录的氧化胆固醇激活剂的激动剂的方法，包括以下步骤：将报道构建体和LXR表达构建体引入宿主细胞; 用潜在的LXR特异性配体处理宿主细胞; 并鉴定激活LXRα转录的化合物。还提供了筛选LXRα转录的氧化胆固醇激活剂的拮抗剂的方法，包括以下步骤：将报道构建体和LXR表达构建体引入宿主细胞; 用LXRα转录激活剂预处理宿主细胞; 使宿主细胞与LXRα转录的潜在拮抗剂接触; 并鉴定阻断LXRα转录激活的化合物。

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