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1. 发明授权

US4356256A Photographic compositions, elements and processes using light-activatable enzymes 失效
标题翻译：使用光激活酶的摄影组合物，元件和方法
公开(公告)号：US4356256A
公开(公告)日：1982-10-26
申请号：US295444
申请日：1981-08-24
申请人： David F. O'Brien , Patricia N. Tyminski
发明人： David F. O'Brien , Patricia N. Tyminski
IPC分类号： G03C1/72 , C12Q1/42 , C12Q1/44 , C12Q1/61 , G03C1/73 , G03C5/24 , G03C1/52
CPC分类号： G03C1/731
摘要： A light-sensitive composition comprising:(1) a plurality of vesicles comprising lipid membranes containing rhodopsin;(2) a mixture of enzymes comprising phosphodiesterase and GTPase;(3) a first nucleotide capable of interacting with GTPase to form a cofactor necessary for the activation of phosphodiesterase;(4) at least one metal cation selected from the group consisting of Mg.sup.+2 and Mn.sup.+2 ; and(5) a second nucleotide capable of being hydrolyzed to produce a proton, said hydrolysis reaction being catalyzed by activated phosphodiesterase, said phosphodiesterase also being activated by rhodopsin exposed by light in the presence of said cofactor and said metal cationis useful in preparing photographic elements comprising means for detecting the hydrolysis reaction, such as an indicator dye. Further, a process for forming an image in the photographic elements comprises imagewise exposing the photographic element to light having a wavelength of 350 to 600 nm, detecting said hydrolysis reaction, and optionally removing the metal cation to render the photographic element insensitive to further exposure.
摘要翻译：一种感光组合物，其包含：（1）包含含有视紫红质的脂质膜的多个囊泡; （2）包含磷酸二酯酶和GTP酶的酶的混合物; （3）能够与GTP酶相互作用以形成磷酸二酯酶活化所必需的辅因子的第一核苷酸; （4）至少一种选自Mg +2和Mn +2的金属阳离子; 所述水解反应由活化的磷酸二酯酶催化，所述磷酸二酯酶也被所述辅因子存在下被光暴露的视紫红质激活，所述金属阳离子可用于制备摄影元件包括用于检测水解反应的装置，例如指示剂染料。此外，用于在照相元件中形成图像的方法包括将照相元件成像曝光至具有350至600nm波长的光，检测所述水解反应，并任选地除去金属阳离子以使照相元件对进一步曝光不敏感。

2. 发明授权

US5527709A Immunoassays with labeled thyronine hapten analogues 失效
标题翻译：带有标记的甲状腺素半抗原类似物的免疫测定
公开(公告)号：US5527709A
公开(公告)日：1996-06-18
申请号：US410548
申请日：1995-03-23
申请人： Susan J. Danielson , Barbara A. Brummond , Patricia N. Tyminski , Ignazio S. Ponticello
发明人： Susan J. Danielson , Barbara A. Brummond , Patricia N. Tyminski , Ignazio S. Ponticello
IPC分类号： C12N9/08 , G01N33/78 , G01N33/545
CPC分类号： C12N9/0065 , G01N33/78 , Y10S436/817
摘要： The invention is directed to an immunoassay for a thyronine derivative comprising:A. contacting a liquid sample, containing a thyronine derivative, with a labeled thyronine hapten analogue of the derivative in the presence of antibodies for the thyronine derivative under conditions that promote the formation of antibody-thyronine immunocomplexes; andB. determining the bound or unbound quantity of the thyronine derivative in the liquid; characterized in that the labeled thyronine derivative comprises:(i) a label, of the type used in immunoassays, having an amine or sulfhydyl group;(ii) a thyronine nucleus and(iii) a linking chain linking the label to the thyronine nucleus.
摘要翻译：本发明涉及一种对于神钢衍生物的免疫测定，其包括：A.在促进形成甲状腺激素的条件下，在含有甲状腺导向抗体的抗体存在下，将含有甲状腺衍生物的液体样品与衍生物的标记的甲状腺恶性半抗原类似物接触抗体 - 甲状腺免疫复合物; 和B.确定液体中的甲状腺衍生物的结合或未结合量; 其特征在于，所述标记的甲状腺衍生物包含：（i）在免疫测定中使用的具有胺或巯基的类型的标签; （ii）神经细胞核和（iii）连接标签与陀螺质核的连接链。

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