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1. 发明申请

US20100029003A1 System and methods for identifying miRNA targets and for altering miRNA and target expression 审中-公开
标题翻译：用于鉴定miRNA靶标和改变miRNA和靶标表达的系统和方法
公开(公告)号：US20100029003A1
公开(公告)日：2010-02-04
申请号：US12321489
申请日：2009-01-21
申请人： David Bartel , Benjamin P. Lewis , Matthew W. Jones-Rhoades , Christopher B. Burge
发明人： David Bartel , Benjamin P. Lewis , Matthew W. Jones-Rhoades , Christopher B. Burge
IPC分类号： C12N15/85
CPC分类号： C12N15/111 , C12N2310/14 , C12N2320/11
摘要： The present invention generally relates to microRNAs such as vertebrate microRNA (miRNA), for example, mammalian miRNA. Various aspects of the invention are directed to the detection, production, or expression of miRNA. In one aspect, the invention provides systems and methods for identifying targets of miRNA sequences. For instance, in one embodiment, gene sequences comprising UTRs are compared with miRNA sequences to determine the degree of interaction, for example, by determining a free energy measurement between the miRNA sequence and the UTR, and/or by determining complementarity between at least a portion of the miRNA sequence and the UTR. In another aspect, the invention is directed to the regulation of gene expression using miRNA. For example, gene expression within a cell may be altered by exposing the cell to an oligonucleotide comprising a sequence that is substantially antisense to at least a portion of an miRNA region of the gene, for example, antisense to a 6-mer or 7-mer portion of the miRNA. In still another aspect, the invention is directed to the treatment of cancer. For instance, in one set of embodiments, an isolated oligonucleotide comprising a sequence that is substantially antisense to an miRNA, or a portion of an miRNA, is administered to a subject having or being at risk of cancer. Yet other aspects of the invention are directed to compositions or kits including oligonucleotides comprising a sequence that is substantially antisense to an miRNA (or a portion of an miRNA), methods of promoting any of the above aspects, or the like.
摘要翻译：本发明通常涉及微小RNA，例如脊椎动物微小RNA（miRNA），例如哺乳动物miRNA。本发明的各个方面涉及miRNA的检测，产生或表达。一方面，本发明提供用于鉴定miRNA序列靶标的系统和方法。例如，在一个实施方案中，将包含UTR的基因序列与miRNA序列进行比较以确定相互作用程度，例如通过确定miRNA序列和UTR之间的自由能测量，和/或通过确定至少一个部分miRNA序列和UTR。另一方面，本发明涉及使用miRNA调节基因表达。例如，可以通过将细胞暴露于寡核苷酸来改变细胞内的基因表达，所述寡核苷酸包含基本上与基因的miRNA区的至少一部分反义的序列，例如与6-聚体或7- miRNA的部分。另一方面，本发明涉及癌症的治疗。例如，在一组实施方案中，将包含基本上与miRNA或一部分miRNA基本反义的序列的分离的寡核苷酸施用于具有或具有癌症风险的受试者。本发明的其它方面涉及包括寡核苷酸的组合物或试剂盒，其包含基本上与miRNA（或miRNA的一部分）反义的序列，促进任何上述方面的方法等。

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