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JP2014102147A Slicing device, slicing method and cell observation method of biological specimen 有权
标题翻译：切片装置，切片方法和生物样本细胞观察方法
公开(公告)号：JP2014102147A
公开(公告)日：2014-06-05
申请号：JP2012254175
申请日：2012-11-20
申请人： Dastech Inc , 株式会社ダステック , Tsukasa Matsumoto , 司松元
发明人： YOSHIDA SHUNJI , TAKAHASHI NOBUYUKI , MATSUMOTO TSUKASA
IPC分类号： G01N1/06 , C12Q1/02 , G01N1/28 , G01N1/30
CPC分类号： G01N1/06 , G01N1/42 , Y10T83/041 , Y10T83/283
摘要： PROBLEM TO BE SOLVED: To hold a cell in a live state and slice a work piece of a biological specimen.SOLUTION: A slicing device for slicing a work piece W of a biological specimen comprises: a substrate 4 on which a work piece W is placed: means for freezing and fixing the work piece W on the substrate 4; and a blade 1 for slicing the work piece W freezed and fixed on the substrate 4 by rotation in a fixed rotation direction r. The shape of the tip 11 of the blade 1 is a curve in which a distance from a rotating shaft C is monotonously increased from an end being the nearest point Pmin having a distance Rmin shortest from the rotating shaft C to the other end being the farthest point Pmax having a distance Rmax longest from the rotating shaft C. The farthest point Pmax is behind positioned in the rotation direction r from the nearest point Pmin, and the curve is convex on the opposite side of the rotating shaft C to a straight line Q connecting the nearest point Pmin and the farthest point Pmax.
摘要翻译：要解决的问题：将细胞保持在活状态并切片生物样品的工件。解决方案：用于切片生物样品的工件W的切片装置包括：基板4，工件W在其上放置：将工件W冷冻固定在基板4上的装置; 以及刀片1，用于通过沿固定旋转方向r旋转将工件W冷冻固定在基板4上。叶片1的尖端11的形状是从旋转轴C的距离从距离旋转轴C最短距离最远的距离Rmin最近的最近点Pmin的端部单调增加到最远的一端的曲线点Pmax距离旋转轴C最远的距离Rmax。最远点Pmax从最近点Pmin开始位于旋转方向r的后方，曲线在旋转轴C的相反侧成直线Q 连接最近点Pmin和最远点Pmax。

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