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1. 发明授权

US06255466B1 Purified plant expansion proteins and DNA encoding same 失效
标题翻译：纯化植物扩增蛋白和编码相同的DNA
公开(公告)号：US06255466B1
公开(公告)日：2001-07-03
申请号：US09092160
申请日：1998-06-05
申请人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
发明人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
IPC分类号： C07H2102
CPC分类号： C12N9/00 , C07K14/415 , C12N9/2437 , C12N15/8241 , C12N15/8242 , C12N15/8261 , D21H17/22 , Y02A40/146
摘要： The sequence is disclosed both as the nucleic acid sequence SEQ ID NO: 1 and as the corresponding amino acid sequence SEQ ID NO: 7. Five other amino acid sequences are also disclosed, two from rice and three from Arabidopsis, SEQ ID NO: 2 through SEQ ID NO:6, respectively.
摘要翻译：该序列同时被公开为核酸序列SEQ ID NO：1，并作为相应的氨基酸序列SEQ ID NO：7。另外还公开了另外5个氨基酸序列，两个来自水稻，三个来自拟南芥，SEQ ID NO：2 通过SEQ ID NO：6。

2. 发明授权

US5959082A Proteins catalyzing the extension of plant cell walls 失效
标题翻译：催化植物细胞壁延伸的蛋白质
公开(公告)号：US5959082A
公开(公告)日：1999-09-28
申请号：US440517
申请日：1995-05-12
申请人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
发明人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
IPC分类号： C07K14/415 , C12N9/00 , C12N9/42 , C12N15/82 , D21H17/22 , A61K35/78 , A23J1/14 , C07K17/00
CPC分类号： C12N9/00 , C07K14/415 , C12N15/8241 , C12N15/8242 , C12N15/8261 , C12N9/2437 , D21H17/22
摘要： A new class of proteins and methods related thereto are presented. The proteins, which can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose, are referred to as expansins. Two proteins have been isolated by fractionation techniques from washed wall fragments of cucumber hypocotyls, referred to as "cucumber expansin-29" and "cucumber expansin-30" (abbreviated cEx-29 and cEx-30, with respect to their apparent relative masses as determined by SDS-PAGE). Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified in Arabidopsis and an additional two in rice. Expansins appear to be broadly distributed throughout the plant kingdom and can be identified in stem and leaf vegetables (i.e., broccoli, cabbage), fruit and seed vegetables (i.e., tomato), fiber crops and cereals (i.e., corn), and forest and ornamental crops (i.e., cotton). An expansin, generally, is a protein which has at least about 60% sequence similarity with the amino acid sequence shown in SEQ. ID. NO:1, and preferably has at least about 70% sequence similarity with SEQ. ID. NO:1.
摘要翻译：提出了一类新的相关蛋白质和方法。可以表征为植物细胞壁延伸的催化剂和纯纤维素中氢键的弱化的蛋白质被称为膨胀物。通过分离技术从黄瓜下胚轴的洗涤壁片段（称为“黄瓜扩展蛋白-29”和“黄瓜扩展子30”）（缩写为cEx-29和cEx-30），通过分离技术分离了两种蛋白质，其相对于其表观相对质量为通过SDS-PAGE测定）。此外，对来自纯化的cEx-29蛋白的三个肽片段进行测序，然后设计寡核苷酸引物以使用来自黄瓜幼苗的cDNA模板进行聚合酶链反应扩增一部分扩展蛋白cDNA，然后使用PCR片段筛选一个cDNA文库，用于鉴定全长克隆。另外一种扩增蛋白已经从燕麦胚芽鞘（oat expansin oEx-29）中分离出来，而在拟南芥中已经鉴定了三种扩增蛋白序列，另外两种在水稻中被鉴定出来。扩展体似乎广泛分布在整个植物界，可以在茎叶蔬菜（即西兰花，卷心菜），水果和种子蔬菜（即番茄），纤维作物和谷类（即玉米），以及森林和观赏作物（即棉花）。通常，扩展蛋白是与SEQ ID NO：1所示氨基酸序列具有至少约60％序列相似性的蛋白质。 ID。 NO：1，优选与SEQ ID NO：1至少约70％的序列相似性。 ID。 NO：1。

3. 发明申请

US20050272041A1 Purified plant expansin proteins and DNA encoding same 失效
标题翻译：纯化植物扩增蛋白和编码相同的DNA
公开(公告)号：US20050272041A1
公开(公告)日：2005-12-08
申请号：US10670009
申请日：2003-09-24
申请人： Daniel Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
发明人： Daniel Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
IPC分类号： C12N9/42 , C07H21/04 , C07K14/415 , C07K14/435 , C12N9/00 , C12N9/64 , C12N15/82 , C12P21/06 , C12Q1/68 , D21C1/00 , D21C5/02 , D21H17/22
CPC分类号： C07K14/415 , C12N9/00 , C12N15/8242 , C12N15/8261 , D21C5/027 , D21H17/22 , Y02A40/146 , Y02W30/648
摘要： This invention relates to a new class of proteins called expansins, and methods related thereto are presented. This class of proteins can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose. Two proteins have been isolated from washed wall fragments of cucumber hypocotyls, referred to as “cucumber expansin-29” and “cucumber expansin-30”. Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified in Arabidopsis and an additional two in rice.
摘要翻译：本发明涉及一类称为膨胀素的新型蛋白质，并提出了与之相关的方法。这类蛋白质可以表征为植物细胞壁延伸的催化剂和纯纤维素中氢键的弱化。已经从黄瓜下胚轴的洗涤的壁碎片中分离出两种蛋白质，称为“黄瓜扩展蛋白-29”和“黄瓜扩展蛋白30”。此外，对来自纯化的cEx-29蛋白的三个肽片段进行测序，然后设计寡核苷酸引物以使用来自黄瓜幼苗的cDNA模板进行聚合酶链反应扩增一部分扩展蛋白cDNA，然后使用PCR片段筛选用于鉴定全长克隆的cDNA文库。另外一种扩增蛋白已经从燕麦胚芽鞘（oat expansin oEx-29）中分离出来，而在拟南芥中已经鉴定了三种扩增蛋白序列，另外两种在水稻中被鉴定出来。

4. 发明授权

US07226756B2 Purified plant expansin proteins and DNA encoding same 失效
标题翻译：纯化植物扩增蛋白和编码相同的DNA
公开(公告)号：US07226756B2
公开(公告)日：2007-06-05
申请号：US10670009
申请日：2003-09-24
申请人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark J. Guiltinan , Tatyana Shcherban , Jun Shi
发明人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark J. Guiltinan , Tatyana Shcherban , Jun Shi
IPC分类号： C07K14/415 , C12N15/82 , C12N9/42 , C12N9/00
CPC分类号： C07K14/415 , C12N9/00 , C12N15/8242 , C12N15/8261 , D21C5/027 , D21H17/22 , Y02A40/146 , Y02W30/648
摘要： This invention relates to a new class of proteins called expansins, and methods related thereto are presented. This class of proteins can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose. Two proteins have been isolated from washed wall fragments of cucumber hypocotyls, referred to as “cucumber expansin-29” and “cucumber expansin-30”. Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified in Arabidopsis and an additional two in rice.
摘要翻译：本发明涉及一类称为膨胀素的新型蛋白质，并提出了与之相关的方法。这类蛋白质可以表征为植物细胞壁延伸的催化剂和纯纤维素中氢键的弱化。已经从黄瓜下胚轴的洗涤的壁碎片中分离出两种蛋白质，称为“黄瓜扩展蛋白-29”和“黄瓜扩展蛋白30”。此外，对来自纯化的cEx-29蛋白的三个肽片段进行测序，然后设计寡核苷酸引物以使用来自黄瓜幼苗的cDNA模板进行聚合酶链反应扩增一部分扩展蛋白cDNA，然后使用PCR片段筛选用于鉴定全长克隆的cDNA文库。另外一种扩增蛋白已经从燕麦胚芽鞘（oat expansin oEx-29）中分离出来，而在拟南芥中已经鉴定了三种扩增蛋白序列，另外两种在水稻中被鉴定出来。

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