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    • 6. 发明授权
    • Determination of adenosine by immunoassay involving acylation of the
adenosine
    • 通过涉及腺苷酰化的免疫测定法测定腺苷
    • US4478934A
    • 1984-10-23
    • US396863
    • 1982-07-09
    • Tomokazu SatoMichio Ui
    • Tomokazu SatoMichio Ui
    • G01N33/53G01N33/537G01N33/54
    • G01N33/537G01N33/5308Y10S436/815
    • A method of quantative determination of adenosine by means, of competitive immunoassay based on a competitive antigen-antibody reaction. In the competitive antigen-antibody reaction, an antibody is used which is obtained from an animal which has been immunized by introduction thereto of an antigen which comprises a carrier protein bonded with 2'- and 3'-hydroxyls of the adenosine through dicarboxylic acid residues, and a labelled adenosine and 2',3'-diacyladenosine which has been produced by acylation of adenosine in the sample to be assayed or in a standard solution are caused to undergo competitive reaction for the antibody whereby it has been made possible to determine adenosine quantitatively in high sensitivity and in high accuracy.
    • 基于竞争性抗原 - 抗体反应的竞争性免疫测定方法,定量测定腺苷的方法。 在竞争性抗原 - 抗体反应中,使用从通过引入抗原免疫的动物获得的抗体,所述抗原包含通过二羧酸残基与腺苷的2'-和3'-羟基键合的载体蛋白 并且通过在待测样品中或在标准溶液中酰化腺苷产生的标记的腺苷和2',3'-二酰基腺苷被引起进行抗体的竞争反应,由此已经可以确定腺苷 定量地在高灵敏度和高精度。