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1. 发明申请

WO2009067569A2 PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING LEUCINE RICH REPEAT KINASE (LLRK) POLYPEPTIDES AND HOMOLOGS THEREOF 审中-公开
标题翻译：具有改变的根构架的植物，涉及编码强力士富重复激酶（LLRK）多肽的基因的相关构建体和方法及其同源物
公开(公告)号：WO2009067569A2
公开(公告)日：2009-05-28
申请号：PCT/US2008084103
申请日：2008-11-20
申请人： DU PONT , PIONEER HI BRED INT , TARAMINO GRAZIANA , TINGEY SCOTT V , SAKAI HAJIME , ALLEN STEPHEN M , TOMES DWIGHT , LUCK STANLEY , NIU XIAOMU
发明人： TARAMINO GRAZIANA , TINGEY SCOTT V , SAKAI HAJIME , ALLEN STEPHEN M , TOMES DWIGHT , LUCK STANLEY , NIU XIAOMU
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8262 , C07K14/415 , C12N9/12 , C12N15/8201 , C12N15/8241 , C12Y207/11001
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译：分离的多核苷酸和多肽以及特别适用于改变植物根部结构的重组DNA构建体，包含这些重组DNA构建体的组合物（如植物或种子），以及利用这些重组DNA构建体的方法。重组DNA构建体包含与在植物中有功能的启动子可操作连接的多核苷酸，其中所述多核苷酸编码可用于改变植物根构造的多肽。

2. 发明申请

WO2010011907A3 PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING REP2 POLYPEPTIDES AND HOMOLOGS THEREOF 审中-公开
标题翻译：具有改变的根构架的植物，涉及编码REP2多肽的基因的相关构建体和方法及其同源物
公开(公告)号：WO2010011907A3
公开(公告)日：2010-03-18
申请号：PCT/US2009051665
申请日：2009-07-24
申请人： DU PONT , PIONEER HI BRED INT , TARAMINO GRAZIANA , TINGEY SCOTT V , ALLEN STEPHEN M , LUCK STANLEY , SAKAI HAJIME , TOMES DWIGHT , NIU XIAOMU
发明人： TARAMINO GRAZIANA , TINGEY SCOTT V , ALLEN STEPHEN M , LUCK STANLEY , SAKAI HAJIME , TOMES DWIGHT , NIU XIAOMU
IPC分类号： C07K14/415 , A01H5/00 , A01H5/10 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译：分离的多核苷酸和多肽以及特别适用于改变植物根部结构的重组DNA构建体，包含这些重组DNA构建体的组合物（如植物或种子），以及利用这些重组DNA构建体的方法。重组DNA构建体包含与在植物中有功能的启动子可操作连接的多核苷酸，其中所述多核苷酸编码可用于改变植物根构造的多肽。

3. 发明申请

WO2009058947A3 PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING EXOSTOSIN FAMILY POLYPEPTIDES AND HOMOLOGS THEREOF 审中-公开
标题翻译：具有改变的根架构的植物，涉及除草剂家族多肽及其同源物的基因的相关构造和方法
公开(公告)号：WO2009058947A3
公开(公告)日：2009-06-25
申请号：PCT/US2008081708
申请日：2008-10-30
申请人： DU PONT , PIONEER HI BRED INT , TARAMINO GRAZIANA , SAKAI HAJIME , TINGEY SCOTT V , ALLEN STEPHEN M , TOMES DWIGHT , LUCK STANLEY , NIU XIAOMU
发明人： TARAMINO GRAZIANA , SAKAI HAJIME , TINGEY SCOTT V , ALLEN STEPHEN M , TOMES DWIGHT , LUCK STANLEY , NIU XIAOMU
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8201 , C12N15/8271 , Y02A40/146
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译：分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。

4. 发明申请

WO2009029739A3 METHODS INVOLVING GENES ENCODING NUCLEOSIDE DIPHOSPHATASE KINASE (NDK) POLYPEPTIDES AND HOMOLOGS THEREOF FOR MODIFYING THE PLANT'S ROOT ARCHITECTURE 审中-公开
标题翻译：涉及编码核苷酸二磷酸酶激酶（NDK）多肽的基因的方法和用于修改植物根系结构的同源物
公开(公告)号：WO2009029739A3
公开(公告)日：2009-06-04
申请号：PCT/US2008074713
申请日：2008-08-29
申请人： DU PONT , PIONEER HI BRED INT , TARAMINO GRAZIANA , TINGEY SCOTT V , SAKAI HAJIME , ALLEN STEPHEN M , TOMES DWIGHT , LUCK STANLEY , NIU XIAOMU
发明人： TARAMINO GRAZIANA , TINGEY SCOTT V , SAKAI HAJIME , ALLEN STEPHEN M , TOMES DWIGHT , LUCK STANLEY , NIU XIAOMU
IPC分类号： A01H5/00 , C12N9/12 , C12N15/82
CPC分类号： C12N15/8273 , C12N9/1229 , C12N15/8262
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译：分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。

5. 发明申请

WO2008100552A3 PLANTS WITH ALTERED ROOT ARCHITECTURE, INVOLVING THE RUM1 GENE, RELATED CONSTRUCTS AND METHODS 审中-公开
标题翻译：涉及RUM1基因的改变根系结构的植物，相关构造和方法
公开(公告)号：WO2008100552A3
公开(公告)日：2009-01-08
申请号：PCT/US2008001927
申请日：2008-02-13
申请人： DU PONT , TARAMINO GRAZIANA , SAKAI HAJIME , KOMATSU MAI , NIU XIAOMU
发明人： TARAMINO GRAZIANA , SAKAI HAJIME , KOMATSU MAI , NIU XIAOMU
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8271 , C12N15/8291 , C12N15/8294 , Y02A40/146
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译：分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。

6. 发明申请

WO2004090143A3 MODULATION OF CYTOKININ ACTIVITY IN PLANTS 审中-公开
标题翻译：植物中细胞因子活性的调节
公开(公告)号：WO2004090143A3
公开(公告)日：2005-05-12
申请号：PCT/US2004010064
申请日：2004-04-02
申请人： PIONEER HI BRED INT , HABBEN JEFFREY E , ZINSELMEIER CHRIS , TOMES DWIGHT T , ABBITT SHANE E , HELENTJARIS TIMOTHY G , NIU XIAOMU
发明人： HABBEN JEFFREY E , ZINSELMEIER CHRIS , TOMES DWIGHT T , ABBITT SHANE E , HELENTJARIS TIMOTHY G , NIU XIAOMU
IPC分类号： C12N9/06 , C12N9/10 , C12N15/29 , C12N15/82 , A01H5/00 , C12N5/10
CPC分类号： C12N9/0032 , C12N9/1085 , C12N15/8234 , C12N15/8261 , Y02A40/146
摘要： This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes that affect metabolically effective levels of cytokinins in plants, particularly in plant seeds and related female reproductive tissue. This invention further relates to transgenic plants having enhanced levels of cytokinin expression wherein the transgenic plant exhibits useful characteristics, such as improved seed size, decreased tip kernel abortion, increased seed set during unfavorable environmental conditions, or stability of yield. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for seed-preferred promoters known as eep1 and eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译：本发明一般涉及植物分子生物学领域。更具体地说，本发明涉及影响植物中特别是植物种子和相关雌性生殖组织中细胞分裂素的代谢有效水平的基因的时间和/或空间调节表达的方法和试剂。本发明进一步涉及具有增强的细胞分裂素表达水平的转基因植物，其中转基因植物表现出有用的特征，例如改善的种子大小，降低的末梢核仁流产，在不利的环境条件下增加的种子集合或产量的稳定性。本发明还提供用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包含已知为eep1和eep2的种子优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

7. 发明申请

WO02078438A3 TISSUE-PREFERRED PROMOTER FROM MAIZE 审中-公开
标题翻译：组织优先促销者从MAIZE
公开(公告)号：WO02078438A3
公开(公告)日：2003-03-20
申请号：PCT/US0209555
申请日：2002-03-28
申请人： PIONEER HI BRED INT , NIU XIAOMU , WANG HAIYIN , TOMES DWIGHT T , HANTKE SABINE
发明人： NIU XIAOMU , WANG HAIYIN , TOMES DWIGHT T , HANTKE SABINE
IPC分类号： C07K14/415 , C12N9/06 , C12N15/82 , A01H1/00 , A01H5/00
CPC分类号： C12N9/0032 , C07K14/415 , C12N15/8223 , C12N15/8295
摘要： The present invention provides a composition and method for regulating expresson of heterologous nucleotide sequences in a plant. The composition is novel nucleic acid sequence for a tissue-preferred promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译：本发明提供了调节植物中异源核苷酸序列表达的组合物和方法。该组合物是用于组织优选启动子的新型核酸序列。还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将植物细胞转化为含有与本发明的组织优选启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

8. 发明申请

WO2006015045A3 METHODS AND COMPOSITIONS FOR MODULATING FLOWERING AND MATURITY IN PLANTS 审中-公开
标题翻译：调查植物花粉和成熟度的方法和组合物
公开(公告)号：WO2006015045A3
公开(公告)日：2008-10-02
申请号：PCT/US2005026641
申请日：2005-07-27
申请人： PIONEER HI BRED INT , UNIV BOLOGNA , TOMES DWIGHT , SALVI SILVIO , MORGANTE MICHELE , SPONZA GIORGIO , BRUGGEMANN EDWARD , NIU XIAOMU , LI BAILIN , TUBEROSA ROBERTO
发明人： TOMES DWIGHT , SALVI SILVIO , MORGANTE MICHELE , SPONZA GIORGIO , BRUGGEMANN EDWARD , NIU XIAOMU , LI BAILIN , TUBEROSA ROBERTO
IPC分类号： C12N15/52 , C12N15/14
CPC分类号： C12N15/827 , C07K14/415
摘要： The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence-based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.
摘要翻译：本发明提供调节植物开花时间的组合物和方法。公开了玉米RAP2.7核苷酸序列，其在过表达导致后期开花并且当被抑制时引起早期开花。还公开了作为RAP2.7调节/增强子的DNA序列，称为VGT1。该序列不编码任何已知的蛋白质，而是作为直接调节开花基因如Rap2.7的表达的RNAi元件或调节DNA或RNA元件，或者特异性靶向控制开花基因的其他基因的表达，例如 Rap2.7。这个元素可以用作基于序列的标记来鉴定已经改变成熟度的近交系和杂交种。提供用于在植物中表达这些核苷酸序列以修饰植物中的成熟和开花的方法以及表达构建体，载体，转化的细胞和植物。

9. 发明申请

WO2006006956A3 ALTERING ROOT STRUCTURE DURING PLANT DEVELOPMENT 审中-公开
标题翻译：改造植物发育过程中的根系结构
公开(公告)号：WO2006006956A3
公开(公告)日：2006-06-01
申请号：PCT/US2005003332
申请日：2005-01-28
申请人： DU PONT , PIONEER HYBRID INTERNAT , TARAMINO GRAZIANA , SAKI HAJIME , MEELEY ROBERT B , NIU XIAOMU
发明人： TARAMINO GRAZIANA , SAKI HAJIME , MEELEY ROBERT B , NIU XIAOMU
IPC分类号： C12N15/00 , A01H5/00
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8227 , Y02A40/146
摘要： Isolated nucleic acid fragments and recombinant constructs comprising such fragments for altering root structure during plant development are disclosed along with methods of root structure alteration during plant development.
摘要翻译：分离的核酸片段和包含用于在植物发育期间改变根结构的这种片段的重组构建体以及在植物发育过程中根结构变化的方法被公开。

10. 发明申请

WO03006651A3 VASCULAR TISSUE PREFERRED PROMOTERS 审中-公开
标题翻译：血管组织优先促销
公开(公告)号：WO03006651A3
公开(公告)日：2003-07-24
申请号：PCT/US0222773
申请日：2002-07-15
申请人： PIONEER HI BRED INT , ABBITT SHANE E , LI CHUN PING , NIU XIAOMU
发明人： ABBITT SHANE E , LI CHUN PING , NIU XIAOMU
IPC分类号： A01H1/00 , A01H5/00 , C12N5/10 , C12N9/24 , C12N15/09 , C12N15/29 , C12N15/82
CPC分类号： C12Y302/01118 , C12N9/2402 , C12N9/2445 , C12N15/8222 , C12N15/8223 , C12Y302/01014
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a vascular tissue-preferred promoter for the gene encoding prunasin hydrolase and sequences isolated therefrom. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the vascular tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码修剪素水解酶的基因的血管组织优选启动子的新型核苷酸序列和从其分离的序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的血管组织优选启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

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