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1. 发明申请

WO2016055578A1 STEVIOL GLYCOSIDE PRODUCTION 审中-公开
标题翻译：硬脂酰甘油生产
公开(公告)号：WO2016055578A1
公开(公告)日：2016-04-14
申请号：PCT/EP2015/073281
申请日：2015-10-08
申请人： DSM IP ASSETS B.V.
发明人： BERG, VAN DEN, Marco Alexander , MADERN, Marcella Katharina , WINTER, Remko Tsjibbe
IPC分类号： C12P19/18 , C12N15/67 , A23L1/236
CPC分类号： C12P19/56 , A23L27/33 , A23L27/36 , A23V2002/00 , C12N15/52 , C12P19/18 , C12Y106/02004 , C12Y114/13078 , C12Y114/13079 , C12Y204/00 , C12Y402/03019 , C12Y505/01013
摘要： The present invention relates to a method for increasing the degree of glycosylation of a composition comprising steviol glycosides, which method comprises: a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof; and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity;a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; a polypeptide having kaurenoic acid 13-hydroxylase activity; and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.
摘要翻译：本发明涉及增加包含甜菊糖苷的组合物的糖基化程度的方法，该方法包括：a。使包含甜菊糖苷的所述组合物与重组微生物接触，来自这样的微生物的无细胞提取物或其衍生物的酶制剂; 和b。从而增加包含甜菊糖苷的组合物的糖基化程度，其中重组微生物包含一个或多个核苷酸序列，其编码：具有ent-copalyl焦磷酸合酶活性的多肽;具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 具有山梨酸13-羟化酶活性的多肽; 和一种或多种具有UDP-葡糖基转移酶活性的多肽，由此使核苷酸序列的表达赋予微生物产生至少一种甜菊糖苷的能力。本发明还涉及包含可通过这种方法获得的甜菊糖苷的组合物。

2. 发明公开

EP3204506A1 STEVIOL GLYCOSIDE PRODUCTION 有权
标题翻译： STEVIOL GLYCOSIDE生产
公开(公告)号：EP3204506A1
公开(公告)日：2017-08-16
申请号：EP15778289.7
申请日：2015-10-08
申请人： DSM IP Assets B.V.
发明人： BERG, VAN DEN, Marco Alexander , MADERN, Marcella Katharina , WINTER, Remko Tsjibbe
IPC分类号： C12P19/18 , C12N15/67
CPC分类号： C12P19/56 , A23L27/33 , A23L27/36 , A23V2002/00 , C12N15/52 , C12P19/18 , C12Y106/02004 , C12Y114/13078 , C12Y114/13079 , C12Y204/00 , C12Y402/03019 , C12Y505/01013
摘要： a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof; and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity;a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; a polypeptide having kaurenoic acid 13-hydroxylase activity; and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.
摘要翻译：本发明涉及用于提高包含甜菊醇糖苷的组合物的糖基化程度的方法，该方法包括：a。使所述包含甜菊醇糖苷的组合物与重组微生物，源自该微生物的无细胞提取物或源自其任一的酶制剂接触; 和b。从而增加包含甜菊醇糖苷的组合物的糖基化程度，其中所述重组微生物包含一种或多种编码以下核苷酸序列的核苷酸序列：具有对焦酰基焦磷酸合酶活性的多肽;具有内 - 胡安烯合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 具有kaurenoic酸13-羟化酶活性的多肽; 和一种或多种具有UDP-葡糖基转移酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物产生至少一种甜菊醇糖苷的能力。本发明还涉及包含通过这种方法可获得的甜菊醇糖苷的组合物。

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