专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2006040313A2 MUTANT EXPANDASES 审中-公开
标题翻译：突变事件
公开(公告)号：WO2006040313A2
公开(公告)日：2006-04-20
申请号：PCT/EP2005055148
申请日：2005-10-11
申请人： DSM IP ASSETS BV , SCHIPPER DICK , KERKMAN RICHARD , RAAMSDONK LOURINA MADELEINE , BOVENBERG ROELOF ARY LANS , JENNE STEPHANE J , CHEN YAN , CHEN YONG HONG , GAO XIAODONG , KREBBER ANKE , KREBBER CLAUS , TO LA CHING CHARLENE , TOBIN MATTHEW
发明人： SCHIPPER DICK , KERKMAN RICHARD , RAAMSDONK LOURINA MADELEINE , BOVENBERG ROELOF ARY LANS , JENNE STEPHANE J , CHEN YAN , CHEN YONG HONG , GAO XIAODONG , KREBBER ANKE , KREBBER CLAUS , TO LA CHING CHARLENE , TOBIN MATTHEW
IPC分类号： C12N9/02 , C12N15/53 , C12P35/00
CPC分类号： C12P35/00 , C12N9/0071
摘要： The present invention relates to a mutant expandase that is a variant of a model polypeptide with expandase activity whereby the mutant expandase has an at least 2-fold improved in vitro expandase activity towards adipyl-6-APA in comparison with the model polypeptide with expandase activity.
摘要翻译：本发明涉及突变扩增酶，其是具有扩展酶活性的模型多肽的变体，其中突变扩增酶与具有扩展酶活性的模型多肽相比具有至己二酰基-6-APA的体外扩增酶活性至少2倍的改善。

2. 发明申请

WO2007006593A3 MUTANT EXPANDASES AND THEIR USE IN THE PRODUCTION OF BETA-LACTAM COMPOUNDS 审中-公开
标题翻译：突变体的发生及其在β-乳糖化合物生产中的应用
公开(公告)号：WO2007006593A3
公开(公告)日：2007-04-12
申请号：PCT/EP2006061479
申请日：2006-04-10
申请人： DSM IP ASSETS BV , SCHIPPER DICK , KERKMAN RICHARD , RAAMSDONK LOURINA MADELEINE , BOVENBERG ROELOF ARY LANS
发明人： SCHIPPER DICK , KERKMAN RICHARD , RAAMSDONK LOURINA MADELEINE , BOVENBERG ROELOF ARY LANS
IPC分类号： C12P37/00 , C12N9/02
CPC分类号： C12P37/00 , C12N9/0069
摘要： The present invention relates to a mutant expandase that is a variant of a model polypeptide with expandase activity whereby the mutant expandase has an at least 2.5-fold improved in vitro expandase activity towards adipyl-6-APA in comparison with a model polypeptide with expandase activity.
摘要翻译：本发明涉及突变扩增酶，其是具有扩展酶活性的模型多肽的变体，其中突变扩增酶与具有扩展酶活性的模型多肽相比具有至己二酰基-6-APA的体外扩增酶活性至少2.5倍的改善。

3. 发明申请

WO0037671A3 IMPROVED IN VIVO PRODUCTION OF CEPHALOSPORINS 审中-公开
标题翻译：改善头孢菌素的体内生产
公开(公告)号：WO0037671A3
公开(公告)日：2000-09-14
申请号：PCT/EP9910292
申请日：1999-12-21
申请人： DSM NV , BOVENBERG ROELOF ARY LANS , KERKMAN RICHARD , KOENHEN ERIC
发明人： BOVENBERG ROELOF ARY LANS , KERKMAN RICHARD , KOENHEN ERIC
IPC分类号： C12P35/06 , C12N9/10 , C12P35/00 , C12R1/82
CPC分类号： C12P35/00 , C12N9/1029
摘要： The present invention discloses a process for the production of 7-amino cephalosporanic acid (7-ACA) or a derivative thereof comprising the steps of fermenting a P. chrysogenum strain being transformed with an expression construct comprising a nucleotide sequence encoding expandase, hydroxylase and acetyltransferase activity in the presence of a suitable acyl side chain precursor, or a salt or ester thereof, such that an N-acylated 7-ACA compound is produced, N-deacylating the thus produced N-acylated 7-ACA compound and, optionally, acylating the free amino group and/or substituting the 3' acetate group with a side chain suitable to form a cephalosporin antibiotic, characterised in that the nucleotide sequence encoding the acetyltransferase is derived from Acremonium chrysogenum and starts at the second ATG of the open reading frame as present in said nucleotide sequence.
摘要翻译：本发明公开了生产7-氨基头孢菌酸（7-ACA）或其衍生物的方法，其包括发酵产黄青霉菌菌株的步骤，所述产黄青霉菌菌株用包含编码扩环酶，羟化酶和乙酰转移酶的核苷酸序列的表达构建体转化在合适的酰基侧链前体或其盐或酯存在下的活性，从而产生N-酰化的7-ACA化合物，将由此产生的N-酰化的7-ACA化合物N-脱酰基化，和任选地酰化游离氨基和/或用适于形成头孢菌素抗生素的侧链取代3'乙酸酯基团，其特征在于编码乙酰转移酶的核苷酸序列来自Acremonium chrysogenum并开始于开放阅读框的第二个ATG，存在于所述核苷酸序列中。

4. 发明申请

WO9802567A3 NOVEL PROCESS FOR THE PREPARATION OF CEPHALOSPORINS USING ACREMONIUM CHRYSOGENUM 审中-公开
标题翻译：使用枸杞子制备碳水化合物的新方法
公开(公告)号：WO9802567A3
公开(公告)日：1998-02-19
申请号：PCT/EP9703878
申请日：1997-07-15
申请人： GIST BROCADES BV , BOVENBERG ROELOF ARY LANS , SCHIPPER DIRK , KERKMAN RICHARD
发明人： BOVENBERG ROELOF ARY LANS , SCHIPPER DIRK , KERKMAN RICHARD
IPC分类号： C12N15/09 , C12N9/10 , C12P35/00 , C12P35/04 , C12R1/75
CPC分类号： C12P35/04 , C12N9/1029
摘要： The present invention describes a process wherein Acremonium is used as a suitable and convenient organism for the production of N-acylated cephalosporin derivatives. In the process of the invention, a recombinant, acyltransferase-expressing Acremonium strain is cultured in the presence of a suitable acyl side chain precursor. Optionally, the acyltransferase-expressing Acremonium strain further does not express hydroxylase and/or acetyltransferase activity. The N-acylated cephalosporin derivative is recovered without contaminating α-aminoadipyl cephalosporin compounds. The thus recovered compound subsequently may be deacylated via chemical or enzymatical means.

5. 发明申请

WO9802551A2 IMPROVED PROCESS FOR THE PRODUCTION OF ADIPOYL CEPHALOSPORINS 审中-公开
标题翻译：用于生产丙烯酰胆碱的改进方法
公开(公告)号：WO9802551A2
公开(公告)日：1998-01-22
申请号：PCT/EP9703879
申请日：1997-07-15
申请人： GIST BROCADES BV , BOVENBERG ROELOF ARY LANS , LAAN JAN METSKE V D , KERKMAN RICHARD , NIEBOER MAARTEN
发明人： BOVENBERG ROELOF ARY LANS , VAN DER LAAN JAN METSKE , KERKMAN RICHARD , NIEBOER MAARTEN
IPC分类号： C12N9/02 , C12P35/02 , C12N15/52 , C12N1/15 , C12N9/00 , C12P35/00
CPC分类号： C12N9/0071 , C12P35/02
摘要： An improved process for the preparation of adipoyl cephalosporins via enzymatic ring expansion of adipoyl-6-aminopenicillinic acid, using a Penicillium chrysogenum transformant strain expressing modified expandase enzyme.
摘要翻译：通过己二酰-6-氨基青霉烯酸的酶环扩展制备己二酰头孢菌素的改进方法，使用表达修饰的扩环酶的产黄青霉转化株。

6. 发明申请

WO2010072765A3 MUTANT PENICILLIN G ACYLASES 审中-公开
公开(公告)号：WO2010072765A3
公开(公告)日：2010-08-26
申请号：PCT/EP2009067752
申请日：2009-12-22
申请人： DSM IP ASSETS BV , LAAN VAN DER JAN METSKE , MOODY HAROLD MONRO , KERKMAN RICHARD , DOES VAN DER THOMAS
发明人： LAAN VAN DER JAN METSKE , MOODY HAROLD MONRO , KERKMAN RICHARD , DOES VAN DER THOMAS
IPC分类号： C12N15/55 , C12N9/84 , C12P35/04 , C12P37/04
CPC分类号： C12N9/84 , C12P35/04 , C12P37/04
摘要： The present invention relates to a mutant prokaryotic penicillin G acylase derived from a wild-type penicillin G acylase characterized in that the mutant is having an amino acid substitution at one or more amino acid positions selected from the group consisting of amino acid positions A3, A77, A90, A144 A192, B24, B109, B148, B313, B460 and B488 according to the amino acid numbering of the Escherichia coli penicillin G acylase having the amino acid sequence depicted in SEQ ID No: 1.
摘要翻译：本发明涉及源自野生型青霉素G酰基转移酶的突变型原核青霉素G酰基转移酶，其特征在于突变体在选自氨基酸位置A3，A77的一个或多个氨基酸位置具有氨基酸取代，A90，A144A192，B24，B109，B148，B313，B460和B488，其具有SEQ ID No：1所示的氨基酸序列的大肠杆菌青霉素G酰基转移酶的氨基酸编号。

7. 发明申请

WO2007073947A3 MUTANT TYPE II BETA- LACTAM ACYLASES 审中-公开
标题翻译：突变型II型乳酸脱氢酶
公开(公告)号：WO2007073947A3
公开(公告)日：2007-11-08
申请号：PCT/EP2006012654
申请日：2006-12-28
申请人： DSM IP ASSETS BV , LAAN VAN DER JAN METSKE , KERKMAN RICHARD , BIJLEVELD WILLEM , GIELESEN BIANCA
发明人： LAAN VAN DER JAN METSKE , KERKMAN RICHARD , BIJLEVELD WILLEM , GIELESEN BIANCA
IPC分类号： C12N9/80
CPC分类号： C12N9/80
摘要： The present invention describes a mutant type Il beta-lactam acylase that is a variant of a model polypeptide with type Il beta-lactam acylase activity whereby the mutant beta-lactam acylase has an at least 1.5-fold improved in vitro beta-lactam acylase activity towards adipyl-7-ADCA in comparison with the model polypeptide with beta-lactam acylase activity.
摘要翻译：本发明描述了II型β-内酰胺酰基转移酶，其是具有II型β-内酰胺酰基转移酶活性的模型多肽的变体，其中突变体β-内酰胺酰基转移酶具有至少1.5倍的体外β-内酰胺酰基转移酶活性与具有β-内酰胺酰基转移酶活性的模型多肽相比，己二酰-7-ADCA。

8. 发明专利

DE60210298T2 未知
公开(公告)号：DE60210298T2
公开(公告)日：2006-11-02
申请号：DE60210298
申请日：2002-07-23
申请人： DSM IP ASSETS BV
发明人： BOVENBERG ARY , KERKMAN RICHARD
IPC分类号： C12N15/10 , C12N9/80 , C12P13/04 , C12P41/00 , C12Q1/68

9. 发明专利

DK1409667T4 未知
公开(公告)号：DK1409667T4
公开(公告)日：2009-09-07
申请号：DK02790188
申请日：2002-07-23
申请人： DSM IP ASSETS BV
发明人： BOVENBERG ROELOF ARY LANS , KERKMAN RICHARD
IPC分类号： C12N15/10 , C07K1/00 , C12N9/80 , C12P13/04 , C12P41/00

10. 发明专利

DE60202478T2 未知
公开(公告)号：DE60202478T2
公开(公告)日：2005-12-29
申请号：DE60202478
申请日：2002-07-23
申请人： DSM IP ASSETS BV
发明人： BOVENBERG ARY , KERKMAN RICHARD
IPC分类号： C07K1/00 , C12N9/80 , C12N15/10 , C12P13/04 , C12P41/00

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式