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1. 发明申请

WO2006133898A3 IMPROVED BIOSYNTETIC PRODUCTION OF 2, 3-TRANS-CHD 审中-公开
标题翻译：改进2，3-TRANS-CHD的生产生产
公开(公告)号：WO2006133898A3
公开(公告)日：2007-10-04
申请号：PCT/EP2006005664
申请日：2006-06-13
申请人： DSM IP ASSETS BV , DSM BIOTECH GMBH , BONGAERTS JOHANNES JOSEF , RAEVEN LEON JEAN RENIER MARIE , SPRENGER GEORG , KUHM ANDREA , TAKORS RALF , BUJNICKI ROBERT PIOTR
发明人： BONGAERTS JOHANNES JOSEF , RAEVEN LEON JEAN RENIER MARIE , SPRENGER GEORG , KUHM ANDREA , TAKORS RALF , BUJNICKI ROBERT PIOTR
IPC分类号： C12P7/42
CPC分类号： C12P7/42
摘要： The invention relates to a process for the biosynthetic production of [5S,6S]-5,6-dihydroxy-cyclohexa-1,3-diene-1-carboxylic acid (2,3-trans-CHD) in an Escherichia microorganism having increased activity of isochorismate synthase and isochorismatase, and being deficient in activity of 2,3-dihydroxybenzoate synthase, wherein the process is carried out in a chorismate overproducing host microorganism also having decreased glucose dehydrogenase activity, or is carried out in a chorismate overproducing host microorganism naturally lacking glucose dehydrogenase activity. In this improved biosynthetic process, 2,3-trans-CHD is being produced in high yields and at concentrations in the biosynthetic reaction medium of at least 12 g/l. The invention further relates to the use of the supernatant of a process for the biosynthetic production of [5S,6S]-5,6-dihydroxy-cyclohexa-1,3-diene-1-carboxylic acid (2,3-trans-CHD) where the concentration of 2,3-trans-CHD in the process has arrived at a concentration of at least 12 g/l as a source for a co-factor for PQQ dependent enzymes.
摘要翻译：本发明涉及在具有增加的大肠杆菌中生物合成生产[5S，6S] -5,6-二羟基 - 环己-1,3-二烯-1-羧酸（2,3-反式-CHD）的方法等位基因合成酶和异激酶活性，并且缺乏2,3-二羟基苯甲酸合成酶的活性，其中该方法在也具有降低的葡萄糖脱氢酶活性的过度生产的宿主微生物中进行，或者在过量产生宿主微生物的天竺葵中进行缺乏葡萄糖脱氢酶活性。在这种改进的生物合成过程中，以高产率和生物合成反应介质中的浓度产生至少12g / l的2,3-反式CHD。本发明还涉及[5S，6S] -5,6-二羟基 - 环己-1,3-二烯-1-羧酸（2,3-反式-CHD）生物合成生产方法的上清液的用途），其中该过程中2,3-反式CHD的浓度已达到至少12g / l的浓度作为PQQ依赖性酶辅因子的来源。

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