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1. 发明申请

WO2008107014A1 QPCR USING SOLID-STATE PH SENSING 审中-公开
标题翻译： QPCR使用固态PH感测
公开(公告)号：WO2008107014A1
公开(公告)日：2008-09-12
申请号：PCT/EP2007/052010
申请日：2007-03-02
申请人： DNA ELECTRONICS LTD , TOUMAZOU, Christofer , PURUSHOTHAMAN, Sunil
发明人： TOUMAZOU, Christofer , PURUSHOTHAMAN, Sunil
IPC分类号： C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6851 , G01N27/4145 , C12Q2565/607 , C12Q2561/113 , C12Q2527/119
摘要： Use of a pH sensor comprising an ion-sensitive field effect transistor (ISFET) to perform real time detection/ quantification of nucleic acid amplification, e.g. polymerase chain reaction (PCR) nucleic acid amplification, based on detection of protons released during the primer extension phase.
摘要翻译：使用包含离子敏感场效应晶体管（ISFET）的pH传感器来执行核酸扩增的实时检测/定量，例如，基于检测在引物延伸期释放的质子的聚合酶链反应（PCR）核酸扩增。

2. 发明申请

WO2003073088A3 APPARATUS AND METHOD FOR THE DETECTION OF LOCALISED FLUCTUACTIONS OF IONIC CHARGE BY ION SENSITIVE FIELD EFFECT TRANSISTORS DURING A CHEMICAL REACTION 审中-公开
公开(公告)号：WO2003073088A3
公开(公告)日：2003-09-04
申请号：PCT/GB2002/000965
申请日：2002-03-11
申请人： TOUMAZOU, Christofer , PURUSHOTHAMAN, Sunil
发明人： TOUMAZOU, Christofer , PURUSHOTHAMAN, Sunil
IPC分类号： G01N27/414
摘要： Sensor and method to detect localised fluctuations of ionic charge indicating events occuring during a chemical reaction, the sensing apparatus comprising an ion sensitive field effect transistor arranged to generate an electrical output signal in response to localised fluctuations of ionic charge at or adjacent the surface of the transistor, and means for detecting the electrical output signal from the field effect transistor is provided with a polymerase linked layer and the chemical reaction is DNA synthesis, and the localised fluctuations of ionic charge indicate the insertion of di-deoxynucleotide triphosphates and deoxynucleotide triphosphates by detecting a change of hydronium ions with a sensitive Si 3 N 4 layer of the field effect transistor.

3. 发明授权

EP2129792B1 QPCR USING AN ION-SENSITIVE FIELD EFFECT TRANSISTOR FOR PH SENSING 有权转让
标题翻译： QPCR使用离子敏感的场效应晶体管作为pH测量
公开(公告)号：EP2129792B1
公开(公告)日：2010-09-01
申请号：EP07726604.7
申请日：2007-03-02
申请人： DNA Electronics Ltd
发明人： TOUMAZOU, Christofer , PURUSHOTHAMAN, Sunil , OU, Chung-Pei
IPC分类号： C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6851 , G01N27/4145 , C12Q2565/607 , C12Q2561/113 , C12Q2527/119
摘要： Use of a pH sensor comprising an ion-sensitive field effect transistor (ISFET) to perform real time detection/ quantification of nucleic acid amplification, e.g. polymerase chain reaction (PCR) nucleic acid amplification, based on detection of protons released during the primer extension phase.

4. 发明公开

EP2211173A1 APPARATUS AND METHOD FOR THE DETECTION OF LOCALISED FLUCTUATIONS OF IONIC CHARGE DURING A CHEMICAL REACTION BY THE USE OF ION SENSITIVE FIELD EFFECT TRANSISTORS 有权
标题翻译：设备和用于检测局部电荷波动的方法，用离子敏感的场效应晶体管的辅助的化学反应过程中
公开(公告)号：EP2211173A1
公开(公告)日：2010-07-28
申请号：EP10157896.1
申请日：2002-03-11
申请人： DNA Electronics Ltd
发明人： TOUMAZOU, Christofer , PURUSHOTHAMAN, Sunil
IPC分类号： G01N27/414 , C12Q1/00 , C12Q1/68
CPC分类号： C12Q1/6825 , C12Q2565/607 , G01N27/4145
摘要： Sensor and method to detect localised fluctuations of ionic charge indicating events occuring during a chemical reaction, the sensing apparatus comprising an ion sensitive field effect transistor (ISFET) arranged to generate an electrical output signal in response to localised fluctuations of ionic charge at or adjacent the surface of the transistor, and means for detecting the electrical output signal from the field effect transistor is provided with a polymerase linked layer and the chemical reaction is DNA synthesis, and the localised fluctuations of ionic charge indicate the insertion of di-deoxynucleotide triphosphates (ddNTP) and deoxynucleotide triphosphates (dNTP) by detecting a change of hydronium ions (pH value) with a sensitive Si 3 N 4 layer of the field effect transistor.
The method is a modification to Sanger sequencing where the final stage of electrophoretic separation and subsequent detection of nucleotides is replaced by measurements of the pH by the ISFET.
摘要翻译：传感器和方法来检测离子电荷指示事件的化学反应过程中存在的的局部波动，所述感测装置包括离子敏感场效应晶体管（ISFET）的布置在电输出信号，以产生响应于离子电荷的局部波动处或附近的所述晶体管的表面，以及用于检测从所述场效应晶体管的电输出信号被提供用聚合酶联层和所述化学反应是DNA合成，和离子电荷的局部波动表明二脱氧核苷酸三磷酸的插入核苷酸（ddNTP ）和脱氧核苷酸三磷酸（的dNTP）通过检测水合氢离子（pH值为）的与敏感的Si 3 N 4层的场效应晶体管的变化。该方法是将Sanger测序，其中电泳分离和核苷酸的后续检测的最后阶段通过将pH值由ISFET测量代替的修改。

5. 发明公开

EP2129792A1 QPCR USING SOLID-STATE PH SENSING 有权转让
标题翻译： QPCR使用离子敏感的场效应晶体管作为pH测量
公开(公告)号：EP2129792A1
公开(公告)日：2009-12-09
申请号：EP07726604.7
申请日：2007-03-02
申请人： DNA Electronics Ltd
发明人： TOUMAZOU, Christofer , PURUSHOTHAMAN, Sunil
IPC分类号： C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6851 , G01N27/4145 , C12Q2565/607 , C12Q2561/113 , C12Q2527/119
摘要： Use of a pH sensor comprising an ion-sensitive field effect transistor (ISFET) to perform real time detection/ quantification of nucleic acid amplification, e.g. polymerase chain reaction (PCR) nucleic acid amplification, based on detection of protons released during the primer extension phase.

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