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1. 发明申请

WO2011008232A3 BIOSYNTHETIC ROUTES TO LONG-CHAIN a, w-HYDROXY ACIDS, DIACIDS AND THEIR CONVERSION TO OLIGOMERS AND POLYMERS 审中-公开
公开(公告)号：WO2011008232A3
公开(公告)日：2011-01-20
申请号：PCT/US2010/001362
申请日：2010-05-06
申请人： DNA 2.0 INC. , POLYTECHNIC INSTITUTE OF NEW YORK UNIVERSITY , GROSS, Richard, A. , LU, Wenhua , NESS, Jon , MINSHULL, Jeremy
发明人： GROSS, Richard, A. , LU, Wenhua , NESS, Jon , MINSHULL, Jeremy
IPC分类号： C12P7/64 , C12P1/02
摘要： A substantially pure Candida host cell for the production of a α-carboxyl-ω- hydroxy fatty acid having a carbon chain length in the range from C6 to C22, a α,ω- dicarboxylic fatty acid having a carbon chain length in the range from C6 to C22, or mixtures thereof, is provided. The Candida host cell is characterized by a first genetic modification class and a second genetic modification class. The first genetic modification class comprises one or more genetic modifications that disrupt the peroxisomal β-oxidation pathway. The second genetic modification class comprises one or more genetic modifications that collectively or individually disrupt at least one gene selected from the group consisting of a CYP52A type cytochrome P450 and a fatty alcohol oxidase.

2. 发明申请

WO2011008231A2 BIOTRANSFORMATION USING GENETICALLY MODIFIED CANDIDA 审中-公开
标题翻译：生物转化使用基因修饰的坎迪达
公开(公告)号：WO2011008231A2
公开(公告)日：2011-01-20
申请号：PCT/US2010/001361
申请日：2010-05-06
申请人： DNA 2.0 INC. , NESS, Jon , MINSHULL, Jeremy
发明人： NESS, Jon , MINSHULL, Jeremy
IPC分类号： C12P7/64 , C12N1/19 , C12N15/74
CPC分类号： C12P7/6409 , C12N9/0006 , C12N15/815 , C12P7/6427 , C12P7/6436 , C12P7/649 , Y02E50/13
摘要： A substantially pure Candida host cell is provided for the biotransformation of a substrate to a product wherein the host cell is characterized by a first genetic modification class that comprises one or more genetic modifications that collectively or individually disrupt at least one alcohol dehydrogenase gene in the substantially pure Candida host cell.
摘要翻译：提供了基本上纯的念珠菌宿主细胞用于将底物生物转化为产物，其中宿主细胞的特征在于第一遗传修饰类别，其包含一个或多个遗传因子在基本上纯的假丝酵母属宿主细胞中共同或单独破坏至少一种醇脱氢酶基因的修饰。

3. 发明申请

WO2014179499A1 METHODS, COMPOSITIONS AND KITS FOR A ONE-STEP DNA CLONING SYSTEM 审中-公开
标题翻译：用于一步DNA克隆系统的方法，组合物和试剂盒
公开(公告)号：WO2014179499A1
公开(公告)日：2014-11-06
申请号：PCT/US2014/036251
申请日：2014-04-30
申请人： DNA TWOPOINTO, INC. , MINSHULL, Jeremy , NESS, Jon , THEODOROU, Elias
发明人： MINSHULL, Jeremy , NESS, Jon , THEODOROU, Elias
IPC分类号： C12P19/34 , C12Q1/68 , C12N9/00 , C12N15/64 , C12N15/66 , C12N15/86
CPC分类号： C12N15/64 , C12N15/66
摘要： Methods and kits for joining two or more polynucleotides to form a product polynucleotide are provided. A mixture contains a first polynucleotide comprising a selectable marker. The mixture further contains a second polynucleotide comprising a first typells recognition sequence and a second typeIIs recognition sequence. The second polynucleotide is other than the first polynucleotide. The mixture further contains a first typells restriction endonuclease that cleaves the first typells recognition sequence to produce a first end, a second typells restriction endonuclease that cleaves the second typells recognition sequence to produce a second end, and a DNA ligase. The first end is not compatible with the second end. The combined actions of the enzymes in the mixture join the first polynucleotide to the second polynucleotide forming a product polynucleotide, which is obtained by transforming the mixture into a host cell.
摘要翻译：提供了用于连接两个或多个多核苷酸以形成产物多核苷酸的方法和试剂盒。混合物含有包含可选择标记的第一多核苷酸。所述混合物还包含第二多核苷酸，其包含第一间隔识别序列和第二II型识别序列。第二多核苷酸不同于第一多核苷酸。该混合物还含有第一个细胞限制性内切核酸酶，其切割第一个分型识别序列以产生第一个末端，第二个间隔限制性内切核酸酶，其切割第二个分型识别序列以产生第二个末端，以及一个DNA连接酶。第一端与第二端不兼容。混合物中酶的组合作用将第一个多核苷酸与形成产物多核苷酸的第二个多核苷酸连接起来，该多核苷酸是通过将混合物转化成宿主细胞得到的。

4. 发明公开

EP2427559A2 BIOTRANSFORMATION USING GENETICALLY MODIFIED CANDIDA 审中-公开
标题翻译：生物转化利用转修饰念珠菌
公开(公告)号：EP2427559A2
公开(公告)日：2012-03-14
申请号：EP10800135.5
申请日：2010-05-06
申请人： DNA TWOPOINTO INC. , Polytechnic Institute of New York University
发明人： NESS, Jon , MINSHULL, Jeremy , GROSS, Richard A , LU, Wenhua
IPC分类号： C12P1/02 , C12N1/00
CPC分类号： C12P7/6409 , C12N9/0006 , C12N15/815 , C12P7/6427 , C12P7/6436 , C12P7/649 , Y02E50/13
摘要： A substantially pure
Candida host cell is provided for the biotransformation of a substrate to a product wherein the host cell is characterized by a first genetic modification class that comprises one or more genetic modifications that collectively or individually disrupt at least one alcohol dehydrogenase gene in the substantially pure
Candida host cell.

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