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1. 发明申请

WO03029425A3 WHOLE CELL ENGINEERING USING REAL-TIME METABOLIC FLUX ANALYSIS 审中-公开
标题翻译：使用实时代谢流量分析的全细胞工程
公开(公告)号：WO03029425A3
公开(公告)日：2003-08-21
申请号：PCT/US0231380
申请日：2002-10-01
申请人： DIVERSA CORP , FU PENCHENG , SHORT JAY M
发明人： FU PENCHENG , SHORT JAY M
IPC分类号： C12N20060101 , C12N15/85 , C12P21/00 , G01N33/48
CPC分类号： C12N15/85
摘要： The invention provides methods for whole cell engineering of new and modified phenotypes by using "on-line" or "real-time" metabolic flux analysis. The invention provides a method for whole cell engineering of new or modified phenotypes by using real-time metabolic flux analysis by making a modified cell by modifying the genetic composition of a cell and culturing the modified cell to generate a plurality of modified cells and measuring at least one metabolic parameter of the cell by monitoring the cell culture of in real time. The invention also provides articles comprising machine-readable medium including machine-executable instructions and systems, e.g., computer systems, to practice the methods of the invention.
摘要翻译：本发明通过使用“在线”或“实时”代谢流分析提供了新细胞和修饰表型的全细胞工程方法。本发明提供了一种通过使用实时代谢流分析通过修饰细胞的遗传组成来修饰细胞并培养修饰细胞以产生多个修饰细胞并在通过实时监测细胞培养物的细胞的至少一个代谢参数。本发明还提供包括机器可读介质的物品，所述机器可读介质包括机器可执行指令和系统（例如计算机系统）以实践本发明的方法。

2. 发明公开

EP1446495A4 WHOLE CELL ENGINEERING USING REAL-TIME METABOLIC FLUX ANALYSIS 审中-公开
标题翻译：建筑安装工程一切细胞中使用代谢河景的实时分析
公开(公告)号：EP1446495A4
公开(公告)日：2006-06-07
申请号：EP02786364
申请日：2002-10-01
申请人： DIVERSA CORP
发明人： FU PENCHENG , SHORT JAY M , LEVIN MICHAEL , LATTERICH MARTIN , WEI JING
IPC分类号： G01N27/62 , A61K48/00 , B01J20/281 , B01J20/285 , C07K1/16 , C12N1/15 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/85 , C12Q1/00 , C12Q1/02 , C12Q1/68 , G01N27/64 , G01N30/04 , G01N30/06 , G01N30/26 , G01N30/34 , G01N30/46 , G01N30/60 , G01N30/72 , G01N30/84 , G01N30/86 , G01N30/88 , G01N31/00 , G01N31/22 , G01N33/48 , G01N33/50 , G01N33/569 , G01N33/58 , G01N33/68 , G06F19/00 , C12P21/00
CPC分类号： G01N33/56911 , G01N33/5091

3. 发明专利

CA2462641A1 WHOLE CELL ENGINEERING USING REAL-TIME METABOLIC FLUX ANALYSIS 未知
公开(公告)号：CA2462641A1
公开(公告)日：2003-04-10
申请号：CA2462641
申请日：2002-10-01
申请人： DIVERSA CORP
发明人： FU PENCHENG , SHORT JAY M
IPC分类号： G01N27/62 , A61K48/00 , B01J20/281 , B01J20/285 , C07K1/16 , C12N1/15 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/85 , C12Q1/00 , C12Q1/02 , C12Q1/68 , G01N27/64 , G01N30/04 , G01N30/06 , G01N30/26 , G01N30/34 , G01N30/46 , G01N30/60 , G01N30/72 , G01N30/84 , G01N30/86 , G01N30/88 , G01N31/00 , G01N31/22 , G01N33/48 , G01N33/50 , G01N33/569 , G01N33/58 , G01N33/68 , G06F19/00 , C12M1/34 , C12N15/01 , C12P21/00 , C12Q1/04 , G01N30/00
摘要： The invention provides methods for whole cell engineering of new and modifie d phenotypes by using "on-line" or "real-time" metabolic flux analysis. The invention provides a method for whole cell engineering of new or modified phenotypes by using real-time metabolic flux analysis by making a modified cell by modifying the genetic composition of a cell and culturing the modifi ed cell to generate a plurality of modified cells and measuring at least one metabolic parameter of the cell by monitoring the cell culture of in real time. The invention also provides articles comprising machine-readable mediu m including machine-executable instructions and systems, e.g., computer system s, to practice the methods of the invention.

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