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1. 发明申请

US20160002686A1 PLANT CELL CULTURE FOR PRODUCTION OF NATURAL PRODUCTS WITH REDUCED FLUCOSINOLATE CONTAMINATION 审中-公开
标题翻译：用于生产具有降低的氟橡胶污染的天然产物的植物细胞培养物
公开(公告)号：US20160002686A1
公开(公告)日：2016-01-07
申请号：US14840258
申请日：2015-08-31
申请人： DIANAPLANTSCIENCES, INC.
发明人： Helena V. Matthews , Sung-Yong Yoon , Mylavarapu Venkatramesh
IPC分类号： C12P17/06 , C12N5/04
CPC分类号： C12P17/06 , A01H4/00 , C12N5/04
摘要： Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.
摘要翻译：本发明公开了一种从甘蓝科植物获得具有降低的硫代葡萄糖苷污染的天然产物制剂的方法。所述方法可以包括从能够产生所需天然产物的植物培养植物愈伤组织，选择具有降低的硫代葡萄糖苷产生的愈伤组织，以及在液体培养基中培养所选择的愈伤组织。该方法还可以包括从培养物中回收天然产物。还公开了获得具有降低的硫代葡萄糖苷污染的甘蓝花青素的方法。该方法可以包括在液体培养基中培养具有降低的硫代葡萄糖苷产量的红甘蓝植物愈伤组织以获得悬浮培养物，并在不含氮源的培养基中培养悬浮培养物。该方法还可以包括从培养物中降低芥子油苷污染来回收花青素。最后，描述了几种特异性低硫代葡萄糖苷酸细胞系。

2. 发明授权

US09206452B2 Plant cell culture for production of natural products with reduced glucosinolate contamination 有权
标题翻译：用于生产具有降低的硫代葡萄糖苷污染的天然产物的植物细胞培养物
公开(公告)号：US09206452B2
公开(公告)日：2015-12-08
申请号：US14062452
申请日：2013-10-24
申请人： DIANAPLANTSCIENCES, INC.
发明人： Helena V. Mathews , Sung-Yong Yoon , Mylavarapu Venkatramesh
IPC分类号： C12N5/00 , C12P17/06 , A01H4/00
CPC分类号： C12P17/06 , A01H4/00 , C12N5/04
摘要： Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.
摘要翻译：本发明公开了一种从甘蓝科植物获得具有降低的硫代葡萄糖苷污染的天然产物制剂的方法。所述方法可以包括从能够产生所需天然产物的植物培养植物愈伤组织，选择具有降低的硫代葡萄糖苷产生的愈伤组织，以及在液体培养基中培养所选择的愈伤组织。该方法还可以包括从培养物中回收天然产物。还公开了获得具有降低的硫代葡萄糖苷污染的甘蓝花青素的方法。该方法可以包括在液体培养基中培养具有降低的硫代葡萄糖苷产量的红甘蓝植物愈伤组织以获得悬浮培养物，并在不含氮源的培养基中培养悬浮培养物。该方法还可以包括从培养物中降低芥子油苷污染来回收花青素。最后，描述了几种特异性低硫代葡萄糖苷酸细胞系。

3. 发明申请

US20140051135A1 PLANT CELL CULTURE FOR PRODUCTION OF NATURAL PRODUCTS WITH REDUCED GLUCOSINOLATE CONTAMINATION 有权
标题翻译：用于生产具有降低的葡萄糖苷酸盐污染的天然产品的植物细胞培养物
公开(公告)号：US20140051135A1
公开(公告)日：2014-02-20
申请号：US14062452
申请日：2013-10-24
申请人： DIANAPLANTSCIENCES, INC.
发明人： Helena V. Mathews , Sung-Yong Yoon , Mylavarapu Venkatramesh
IPC分类号： C12P17/06
CPC分类号： C12P17/06 , A01H4/00 , C12N5/04
摘要： Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.
摘要翻译：本发明公开了一种从甘蓝科植物获得具有降低的硫代葡萄糖苷污染的天然产物制剂的方法。所述方法可以包括从能够产生所需天然产物的植物培养植物愈伤组织，选择具有降低的硫代葡萄糖苷产生的愈伤组织，以及在液体培养基中培养所选择的愈伤组织。该方法还可以包括从培养物中回收天然产物。还公开了获得具有降低的硫代葡萄糖苷污染的甘蓝花青素的方法。该方法可以包括在液体培养基中培养具有降低的硫代葡萄糖苷产量的红甘蓝植物愈伤组织以获得悬浮培养物，并在不含氮源的培养基中培养悬浮培养物。该方法还可以包括从培养物中降低芥子油苷污染来回收花青素。最后，描述了几种特异性低硫代葡萄糖苷酸细胞系。

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