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1. 发明申请

WO2006045591A3 METHOD AND CONSTRUCTS FOR DELIVERING DOUBLE STRANDED RNA TO PEST ORGANISMS 审中-公开
标题翻译：用于将双重条纹RNA递送到有机物的方法和结构
公开(公告)号：WO2006045591A3
公开(公告)日：2006-06-15
申请号：PCT/EP2005011441
申请日：2005-10-25
申请人： DEVGEN NV , BOGAERT THIERRY ANDRE OLIVIER , ZWAAL RICHARD , PLAETINCK GEERT , DE KERPEL JAN OCTAAF , KALETTA TITUS JAN
发明人： BOGAERT THIERRY ANDRE OLIVIER , ZWAAL RICHARD , PLAETINCK GEERT , DE KERPEL JAN OCTAAF , KALETTA TITUS JAN
IPC分类号： C12N15/82 , A61K38/16 , C12N15/00 , C12N15/09 , C12N15/62
CPC分类号： C12N15/8279 , A01K2217/058 , A01K2227/703 , A61K48/0033 , C12N15/111 , C12N15/113 , C12N15/8216 , C12N15/8283 , C12N15/8285 , C12N15/8286 , C12N2310/14 , C12N2310/3513 , C12N2320/32 , C12N2710/14143 , C12N2770/00011 , Y02A40/162 , Y02A40/164
摘要： The present invention relates in generally to RNAi and its use in gene silencing. In particular, the present invention relates to methods and constructs for delivering double stranded RNA (dsRNA) to pest organisms.
摘要翻译：本发明一般涉及RNAi及其在基因沉默中的用途。特别地，本发明涉及用于将双链RNA（dsRNA）递送给有害生物的方法和构建体。

2. 发明申请

WO2006046148A2 RNA CONSTRUCTS 审中-公开
标题翻译： RNA结构
公开(公告)号：WO2006046148A2
公开(公告)日：2006-05-04
申请号：PCT/IB2005003557
申请日：2005-10-25
申请人： DEVGEN NV , VAN DE CRAEN MARC , PLAETINCK GEERT , VERCAUTEREN ISABELLE , LOGGHE MARC GEORGES , BOGAERT THIERRY ANDRE OLIVIER , ZWAAL RICHARD
发明人： VAN DE CRAEN MARC , PLAETINCK GEERT , VERCAUTEREN ISABELLE , LOGGHE MARC GEORGES , BOGAERT THIERRY ANDRE OLIVIER , ZWAAL RICHARD
IPC分类号： C12N15/11 , C12N15/113
CPC分类号： C12N15/8285 , C12N15/111 , C12N15/113 , C12N15/8279 , C12N2310/14 , C12N2310/3519 , C12N2330/30
摘要： The present invention concerns concatemer and/or stabilized RNA constructs capable of forming dsRNA, optionally comprising a sequence capable of protecting the dsRNA against RNA processing in a host cell. The invention also relates to methods of producing these constructs and to methods for using these constructs. The constructs according to the present invention are particularly useful in plant pest control.
摘要翻译：本发明涉及能够形成dsRNA的并联和/或稳定的RNA构建体，任选地包含能够保护dsRNA的序列免受宿主细胞中的RNA加工。本发明还涉及生产这些构建体的方法和使用这些构建体的方法。根据本发明的构建体在植物害虫防治中特别有用。

3. 发明申请

WO2006045590A3 MULTIDOMAIN RNA MOLECULES COMPRISING AT LEAST ONE APTAMER FOR DELIVERING DOUBLE STRANDED RNA TO PEST ORGANISMS 审中-公开
标题翻译：包含至少一种APTAMER的多肽RNA分子，用于将双重条纹RNA递送到PEST有机体
公开(公告)号：WO2006045590A3
公开(公告)日：2006-06-15
申请号：PCT/EP2005011439
申请日：2005-10-25
申请人： DEVGEN NV , BOGAERT THIERRY ANDRE OLIVIER , FELDMANN PASCALE , PLAETINCK GEERT
发明人： BOGAERT THIERRY ANDRE OLIVIER , FELDMANN PASCALE , PLAETINCK GEERT
IPC分类号： A01H5/00 , C12N15/11 , C12N15/113 , C12N15/82
CPC分类号： C12N15/111 , C12N15/113 , C12N15/8218 , C12N15/8279 , C12N15/8282 , C12N15/8285 , C12N15/8286 , C12N2310/14 , C12N2310/16 , C12N2310/3519 , C12N2320/32 , Y02A40/162 , Y02A40/164
摘要： The present invention relates to methods and constructs for delivering double stranded RNA (dsRNA) to pest organisms. More specific, the invention relates to a multidomain RNA molecule consisting of a nucleotide sequence comprising (i) at least one aptamer and (ii) at least one nucleotide sequence of interest forming double-stranded RNA, said double-stranded RNA comprising annealed complementary strands, one of which comprises a nucleotide sequence which is complementary to at least part a pest target nucleotide sequence. The invention further relates to nucleic acids encoding the multidomain RNA molecules and to various uses of the multidomain RNA molecules in agriculture.
摘要翻译：本发明涉及用于将双链RNA（dsRNA）递送给有害生物的方法和构建体。更具体地，本发明涉及由以下核苷酸序列组成的多结构域RNA分子：核苷酸序列，其包含（i）至少一个适体和（ii）至少一个目标核苷酸序列形成双链RNA，所述双链RNA包括退火互补链其中之一包含与至少部分害虫靶核苷酸序列互补的核苷酸序列。本发明还涉及编码多区RNA分子的核酸以及多域RNA分子在农业中的各种用途。

4. 发明申请

WO2007074405A3 DSRNA AS INSECT CONTROL AGENT 审中-公开
公开(公告)号：WO2007074405A3
公开(公告)日：2008-04-17
申请号：PCT/IB2006004003
申请日：2006-09-18
申请人： DEVGEN NV , RAEMAEKERS ROMAAN , FELDMANN PASCALE , PLAETINCK GEERT , NOOREN IRENE , VAN BLEU ELS , PECQUEUR FREDERIC
发明人： RAEMAEKERS ROMAAN , FELDMANN PASCALE , PLAETINCK GEERT , NOOREN IRENE , VAN BLEU ELS , PECQUEUR FREDERIC
IPC分类号： C12N15/82 , A01H5/00 , C12N5/10 , C12N15/11 , C12N15/12
CPC分类号： C12N15/8286 , A01N57/10 , C12N15/113 , C12N15/8218 , C12N15/8281 , C12N15/8282 , C12N15/8283 , C12N15/8285 , C12N2310/14 , Y02A40/162 , Y02A40/164
摘要： The present invention relates to methods for controlling pest infestation using double stranded RNA molecules. The invention provides methods for making transgenic plants that express the double stranded RNA molecules, as well as pesticidal agents and commodity products produced by the inventive plants.

5. 发明申请

WO2007080127A3 DSRNA AS INSECT CONTROL AGENT 审中-公开
标题翻译： DSRNA作为控制剂
公开(公告)号：WO2007080127A3
公开(公告)日：2008-03-27
申请号：PCT/EP2007000287
申请日：2007-01-12
申请人： DEVGEN NV , RAEMAEKERS ROMAAN , KUBLER LAURENT , PLAETINCK GEERT , VANBLEU ELS
发明人： RAEMAEKERS ROMAAN , KUBLER LAURENT , PLAETINCK GEERT , VANBLEU ELS
IPC分类号： C12N15/82 , A01H5/00 , C12N5/10 , C12N15/11 , C12N15/12
CPC分类号： C12N15/8286 , A01N57/16 , A01N61/00 , C07K14/43527 , C07K14/4354 , C12N15/8282 , C12N15/8285 , Y02A40/162 , Y02A40/164
摘要： The present invention concerns methods for controlling insect infestation via RNAi- mediated gene silencing, whereby the intact insect cell(s) are contacted with a double-stranded RNA from outside the insect cell(s) and whereby the double-stranded RNA is taken up by the intact insect cell(s). In one particular embodiment, the methods of the invention are used to alleviate plants from insect pests. Alternatively, the methods are used for treating and/or preventing insect infestation on a substrate or a subject in need of such treatment and/or prevention. Suitable insect target genes and fragments thereof, dsRNA constructs, recombinant constructs and compositions are disclosed.
摘要翻译：本发明涉及通过RNAi介导的基因沉默来控制昆虫侵袭的方法，其中完整的昆虫细胞与来自昆虫细胞外的双链RNA接触，由此将双链RNA吸收通过完整的昆虫细胞。在一个具体实施方案中，本发明的方法用于减轻植物害虫。或者，所述方法用于治疗和/或预防对需要这种治疗和/或预防的基质或受试者的昆虫侵袭。公开了合适的昆虫靶基因及其片段，dsRNA构建体，重组构建体和组合物。

6. 发明申请

WO2007083193A3 METHODS FOR CONTROLLING PESTS USING RNAI 审中-公开
标题翻译：使用RNAI控制PESTS的方法
公开(公告)号：WO2007083193A3
公开(公告)日：2008-01-24
申请号：PCT/IB2006004008
申请日：2006-09-18
申请人： DEVGEN NV , RAEMAEKERS ROMAAN , FELDMANN PASCALE , PLAETINCK GEERT , NOOREN IRENE , BLEU ELS VAN , PECQUEUR FREDERIC , KUBLER LAURENT , DAMME NICOLE , DEGRAVE LIES , REMORY ISABEL
发明人： RAEMAEKERS ROMAAN , FELDMANN PASCALE , PLAETINCK GEERT , NOOREN IRENE , BLEU ELS VAN , PECQUEUR FREDERIC , KUBLER LAURENT , DAMME NICOLE , DEGRAVE LIES , REMORY ISABEL
IPC分类号： C12N15/82 , A01H5/00 , C12N5/10 , C12N15/11 , C12N15/12
CPC分类号： C12N15/113 , A01N63/02 , C12N15/8286 , C12N2310/14 , Y02A40/162
摘要： The present invention relates to methods for controlling pest infestation using double stranded RNA molecules. The invention provides methods for producing transgenic cells expressing the double stranded RNA molecules, as well as compositions and commodity products containing or treated with such molecules.
摘要翻译：本发明涉及使用双链RNA分子控制害虫侵染的方法。本发明提供了用于产生表达双链RNA分子的转基因细胞的方法，以及含有或用这些分子处理的组合物和商品。

7. 发明申请

WO0148183A3 IMPROVEMENTS RELATING TO DOUBLE-STRANDED RNA INHIBITION 审中-公开
标题翻译：关于双重条纹RNA抑制的改进
公开(公告)号：WO0148183A3
公开(公告)日：2001-12-06
申请号：PCT/EP0013149
申请日：2000-12-22
申请人： DEVGEN NV , PLAETINCK GEERT , MORTIER KATHERINE , LISSENS ANN , BOGAERT THIERRY
发明人： PLAETINCK GEERT , MORTIER KATHERINE , LISSENS ANN , BOGAERT THIERRY
IPC分类号： C07K14/435 , C12N15/10 , C12N1/00 , C12N9/22 , C12N15/66 , C12N15/70
CPC分类号： C07K14/43545
摘要： There are described ways of improving the efficiency of double stranded RNA inhibition as a method of inhibiting gene expression in nematode worms such as C. elegans . In particular, the invention relates to the finding that changes in the genetic background of C. elegans result in increased sensitivity to double-stranded RNA inhibition.
摘要翻译：描述了提高双链RNA抑制效率的方法，作为抑制线虫（例如C）中基因表达的方法。线虫的。特别地，本发明涉及到C的遗传背景的变化的发现。线虫导致对双链RNA抑制的敏感性增加。

8. 发明申请

WO0001846A3 CHARACTERISATION OF GENE FUNCTION USING DOUBLE STRANDED RNA INHIBITION 审中-公开
标题翻译：用双链RNA抑制法表征基因功能
公开(公告)号：WO0001846A3
公开(公告)日：2000-06-15
申请号：PCT/EP9904718
申请日：1999-07-02
申请人： DEVGEN NV , PLAETINCK GEERT , PLATTEEUW CHRIST , MORTIER KATHERINE , BOGAERT THIERRY
发明人： PLAETINCK GEERT , PLATTEEUW CHRIST , MORTIER KATHERINE , BOGAERT THIERRY
IPC分类号： C12N15/09 , A01K67/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/10 , C12N15/63 , C12N15/82 , C12N15/86 , C12Q20060101 , C12Q1/68
CPC分类号： C12N15/1079 , C12N15/1093 , C12N15/8216 , C12N15/8218 , C12N15/8279 , C12N15/8285 , C12N15/86 , C12N2795/10243 , C12N2800/40 , C12N2830/002 , C12N2830/008 , C12N2830/42 , C12Q1/68 , Y02A40/164
摘要： There is provided a method of identifying DNA responsible for conferring a particular phenotype in a cell which method comprises a) constructing a cDNA or genomic library of the DNA of said cell in a suitable vector in an orientation relative to a promoter(s) capable of initiating transcription of said cDNA or DNA to double stranded (ds) RNA upon binding of an appropriate transcription factor to said promoter(s), b) introducing said library into one or more of said cells comprising said transcription factor, and c) identifying and isolating a particular phenotype of said cell comprising said library and identifying the DNA or cDNA fragment from said library responsible for conferring said phenotype. Using this technique it is also possible to assign function to a known DNA sequence by a) identifying a homologue(s) of said DNA sequence in a cell, b) isolating the relevant DNA homologue(s) or a fragment thereof from said cell, c) cloning said homologue or fragment thereof into an appropriate vector in an orientation relative to a suitable promoter(s) capable of initiating transcription of dsRNA from said DNA homologue or fragment upon binding of an appropriate transcription factor to said promoter(s) and d) introducing said vector into said cell from step a) comprising said transcription factor.
摘要翻译：提供了鉴定负责赋予细胞中特定表型的DNA的方法，该方法包括a）在合适的载体中以相对于启动子的方向构建所述细胞的DNA的cDNA或基因组文库，所述启动子能够在将适当的转录因子与所述启动子结合后，启动所述cDNA或DNA转录成双链（ds）RNA，b）将所述文库引入一个或多个包含所述转录因子的所述细胞中，以及c）鉴定并分离包含所述文库的所述细胞的特定表型，并从所述文库中鉴定出赋予所述表型的DNA或cDNA片段。使用该技术，还可以通过a）鉴定细胞中所述DNA序列的同源物，b）从所述细胞中分离相关的DNA同源物或其片段， c）将所述同源物或其片段以合适的启动子的方向克隆到合适的载体中，所述启动子能够从适当的转录因子与所述启动子结合后从所述DNA同源物或片段启动dsRNA的转录，和d ）将所述载体导入步骤a）的所述细胞中，所述细胞包含所述转录因子。

9. 发明申请

WO0188114A3 GENE EXPRESSION SYSTEM 审中-公开
标题翻译：基因表达系统
公开(公告)号：WO0188114A3
公开(公告)日：2002-06-20
申请号：PCT/EP0105794
申请日：2001-05-18
申请人： DEVGEN NV , PLAETINCK GEERT , RENARD JEAN-PIERRE , MORTIER KATHERINE , BOGAERT THIERRY
发明人： PLAETINCK GEERT , RENARD JEAN-PIERRE , MORTIER KATHERINE , BOGAERT THIERRY
IPC分类号： C12N15/85 , C12N15/00
CPC分类号： C12N15/85 , A01K2217/05 , C12N2830/00 , C12N2830/75 , C12N2840/445
摘要： There are provided DNA constructs, including replicable cloning vectors and expression vectors, comprising a bacteriophage promoter operably linked to an outron sequence. The expression vectors provided by the invention are useful in the expression of recombinant polypeptides in host cells or organisms and are particularly useful in expression of recombinant polypeptides in nematode worms such as C. elegans .
摘要翻译：提供了DNA构建体，包括可复制克隆载体和表达载体，其包含可操作地连接到外源序列的噬菌体启动子。本发明提供的表达载体可用于在宿主细胞或生物体中表达重组多肽，并且特别可用于在线虫例如C中重组多肽的表达。线虫的。

10. 发明专利

JP2012228256A Vector construct 有权
标题翻译：矢量构造
公开(公告)号：JP2012228256A
公开(公告)日：2012-11-22
申请号：JP2012151889
申请日：2012-07-05
申请人： Devgen Nv , デフヘン・ナムローゼ・フェンノートシャップDEVGEN nv
发明人： PLAETINCK GEERT , RENARD JEAN-PIERRE , BOGAERT THIERRY
IPC分类号： C12N15/09 , C12N1/21 , C12N15/113 , C12N15/63 , C12N15/70 , C12P19/34 , C12R1/19
CPC分类号： C12N15/63 , C12N15/70 , C12P19/34
摘要： PROBLEM TO BE SOLVED: To provide a vector construct useful in the expression of double-stranded RNA.SOLUTION: The DNA construct comprises (a) a first promoter and (b) a second promoter, wherein the first promoter and the second promoter are in opposite orientation to each other. Additionally the construct comprises (c) an inter-promoter region that is located downstream of the 3' terminal of the first promoter and downstream of the 3' terminal of the second promoter and defined, (d) at least one cloning site located in the inter-promoter region, and (e) a first transcription terminator which is located downstream of the first promoter and downstream of at least one cloning site when viewed from the 3' terminal side of the first promoter. The first transcription terminator is operably linked to the first promoter. The construct is useful for expression of double-stranded RNA in vitro and in vivo.
摘要翻译：待解决的问题：提供可用于双链RNA表达的载体构建体。解决方案：DNA构建体包含（a）第一启动子和（b）第二启动子，其中第一启动子和第二启动子彼此相反取向。另外，构建体包含（c）位于第一启动子的3'末端下游并在第二启动子的3'末端下游的启动子间区域，并定义了（d）至少一个位于第启动子区域，和（e）当从第一启动子的3'末端侧观察时，位于第一启动子下游的第一转录终止子和至少一个克隆位点的下游。第一转录终止子可操作地连接到第一启动子。该构建体可用于在体外和体内表达双链RNA。版权所有（C）2013，JPO＆INPIT

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