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1. 发明申请

WO1998026064A2 METHOD FOR ALTERING THE NUTRITIONAL CONTENT OF PLANT SEED 审中-公开
标题翻译：改变植物种子营养成分的方法
公开(公告)号：WO1998026064A2
公开(公告)日：1998-06-18
申请号：PCT/US1997022744
申请日：1997-12-09
申请人： DEKALB GENETICS CORPORATION
发明人： DEKALB GENETICS CORPORATION , KIRIHARA, Julie, A. , HIBBERD, Kenneth, A. , ANTHONY, Janice
IPC分类号： C12N15/11
CPC分类号： C12N15/8251 , C07K14/425 , C12N15/8218 , C12N15/8245 , C12N15/8253 , C12N15/8254
摘要： The invention provides genetically engineered, preselected DNA sequences and methods of using them to alter the nutritional content of plant seed. Methods of the invention are directed to increasing the weight percent of at least one amino acid essential to the diet of animals, or increasing the starch content of a plant. One such method involves stably transforming a cell of a plant with a preselected DNA sequence encoding a RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, preferably a seed storage protein which is deficient in at least one amino acid essential to the diet of animals. An alternative method employs stably transforming cells with at least two preselected DNA sequences, one of which encodes an RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, and the other preselected DNA molecule which encodes a preselected polypeptide. The transformed cells are used to generate fertile transgenic plants and seeds. Transgenic seeds are characterized by expression of the preselected DNA sequence which results in a substantial inhibition of production of a seed storage protein deficient in at least one amino acid essential to the diet of animals and/or an increase in the weight percent of an amino acid essential to the diet of animals.
摘要翻译：本发明提供遗传工程化的预选DNA序列和使用它们改变植物种子营养含量的方法。本发明的方法涉及增加动物饮食必需的至少一种氨基酸的重量百分数，或增加植物的淀粉含量。一种这样的方法包括用编码与编码植物种子储存蛋白的信使RNA（mRNA）基本上相同或互补的RNA分子的预选DNA序列稳定转化植物细胞，优选地存在至少一种缺失的种子储存蛋白对动物饮食必不可少的氨基酸。一种替代方法使用至少两个预选的DNA序列稳定转化细胞，其中之一编码与编码植物种子储存蛋白的信使RNA（mRNA）基本相同或互补的RNA分子，以及编码预先选择的DNA分子多肽。转化细胞用于产生可育转基因植物和种子。转基因种子的特征在于预选的DNA序列的表达，其导致对动物饮食必需的至少一个氨基酸缺乏的种子储存蛋白的产生的显着抑制和/或氨基酸的重量百分比的增加对动物的饮食至关重要

2. 发明公开

EP0942971A2 METHOD FOR ALTERING THE NUTRITIONAL CONTENT OF PLANT SEED 失效
标题翻译：程序在植物种子食品的含量变化
公开(公告)号：EP0942971A2
公开(公告)日：1999-09-22
申请号：EP97954090.0
申请日：1997-12-09
申请人： DeKalb Genetics Corporation
发明人： KIRIHARA, Julie, A. , HIBBERD, Kenneth, A. , ANTHONY, Janice
IPC分类号： A01H5 , C07K14 , C12N5 , C12N15 , C12P19 , C12P21
CPC分类号： C12N15/8251 , C07K14/425 , C12N15/8218 , C12N15/8245 , C12N15/8253 , C12N15/8254
摘要： The invention provides genetically engineered, preselected DNA sequences and methods of using them to alter the nutritional content of plant seed. Methods of the invention are directed to increasing the weight percent of at least one amino acid essential to the diet of animals, or increasing the starch content of a plant. One such method involves stably transforming a cell of a plant with a preselected DNA sequence encoding a RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, preferably a seed storage protein which is deficient in at least one amino acid essential to the diet of animals. An alternative method employs stably transforming cells with at least two preselected DNA sequences, one of which encodes an RNA molecule substantially identical or complementary to a messenger RNA (mRNA) encoding a plant seed storage protein, and the other preselected DNA molecule which encodes a preselected polypeptide. The transformed cells are used to generate fertile transgenic plants and seeds. Transgenic seeds are characterized by expression of the preselected DNA sequence which results in a substantial inhibition of production of a seed storage protein deficient in at least one amino acid essential to the diet of animals and/or an increase in the weight percent of an amino acid essential to the diet of animals.

3. 发明授权

EP0942971B1 METHOD FOR ALTERING THE NUTRITIONAL CONTENT OF PLANT SEED 失效
标题翻译：程序在植物种子食品的含量变化
公开(公告)号：EP0942971B1
公开(公告)日：2010-10-13
申请号：EP97954090.3
申请日：1997-12-09
申请人： DeKalb Genetics Corporation
发明人： KIRIHARA, Julie, A. , HIBBERD, Kenneth, A. , ANTHONY, Janice
IPC分类号： C12N15/11 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8251 , C07K14/425 , C12N15/8218 , C12N15/8245 , C12N15/8253 , C12N15/8254

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