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1. 发明专利

ZA200809448B Enzyme-microbe synergy 未知
公开(公告)号：ZA200809448B
公开(公告)日：2010-08-25
申请号：ZA200809448
申请日：2008-11-05
申请人： DARTMOUTH COLLEGE
发明人： ZHANG YI-HENG PERCIVAL , LU YANPIN , LYND LEE R
IPC分类号： C12P20060101

2. 发明专利

CA2651753A1 ENZYME-MICROBE SYNERGY 未知
公开(公告)号：CA2651753A1
公开(公告)日：2007-11-29
申请号：CA2651753
申请日：2007-05-01
申请人： DARTMOUTH COLLEGE , LYND LEE R
发明人： ZHANG YI-HENG PERCIVAL , LYND LEE R , LU YANPIN
IPC分类号： C12P19/14
摘要： Methods of utilizing reduced cellulase loads to hydrolyze cellulosic subs trates are disclosed. The methods include determining an amount of purified cellulase necessary to substantially hydrolyze a quantity of cellulosic subs trate in a period of time; reducing the amount of purified cellulase by a fa ctor of between 2 and 5 to determine a reduced cellulase load; and introduci ng to the cellulosic substrate either (1 ) a microorganism expressing cell-b ound cellulase in a concentration equal to the reduced cellulase load or (2) a fermentation agent that has been engineered to express cell-bound cellula se in a concentration equal to the reduced cellulase load under suitable con ditions and for said period of time sufficient to allow substantial hydrolys is of the cellulosic substrate.

3. 发明专利

AU2007253987A1 Process for the hydrolysis of cellulose mediated by ternary complexes of cellulose, clostridium thermocellum cells, and cellulase expressed by these cells 未知
公开(公告)号：AU2007253987A1
公开(公告)日：2007-11-29
申请号：AU2007253987
申请日：2007-05-01
申请人： LYND LEE R , DARTMOUTH COLLEGE
发明人： LYND LEE R , LU YANPIN , ZHANG YI-HENG PERCIVAL
IPC分类号： C12P19/14

4. 发明专利

BRPI0711163A2 未知
公开(公告)号：BRPI0711163A2
公开(公告)日：2011-08-23
申请号：BRPI0711163
申请日：2007-05-01
申请人： DARTMOUTH COLLEGE , LYND LEE R
发明人： LYND LEE R , ZHANG YI-HENG PERCIVAL , LU YANPIN
IPC分类号： C12P19/14

5. 发明申请

WO2007136971A3 PROCESS FOR THE HYDROLYSIS OF CELLULOSE MEDIATED BY TERNARY COMPLEXES OF CELLULOSE, CLOSTRIDIUM THERMOCELLUM CELLS, AND CELLULASE EXPRESSED BY THESE CELLS 审中-公开
标题翻译：由细胞周期性复合物介导的纤维素水解的方法，这些细胞表达的细胞因子和细胞因子
公开(公告)号：WO2007136971A3
公开(公告)日：2008-04-10
申请号：PCT/US2007067954
申请日：2007-05-01
申请人： DARTMOUTH COLLEGE , LYND LEE R , ZHANG YI-HENG PERCIVAL , LU YANPIN
发明人： LYND LEE R , ZHANG YI-HENG PERCIVAL , LU YANPIN
IPC分类号： C12P19/14
CPC分类号： C12P19/14
摘要： Methods of utilizing reduced cellulase loads to hydrolyze cellulosic substrates are disclosed. The methods include determining an amount of purified cellulase necessary to substantially hydrolyze a quantity of cellulosic substrate in a period of time; reducing the amount of purified cellulase by a factor of between 2 and 5 to determine a reduced cellulase load; and introducing to the cellulosic substrate either (1 ) a microorganism expressing cell-bound cellulase in a concentration equal to the reduced cellulase load or (2) a fermentation agent that has been engineered to express cell-bound cellulase in a concentration equal to the reduced cellulase load under suitable conditions and for said period of time sufficient to allow substantial hydrolysis of the cellulosic substrate.
摘要翻译：公开了利用降低的纤维素酶负载来水解纤维素基质的方法。所述方法包括确定在一段时间内基本上水解一定量的纤维素基质所需的纯化纤维素酶的量; 将纯化的纤维素酶的量减少2至5倍以确定纤维素酶负荷降低; 并且向纤维素底物引入（1）表达细胞结合的纤维素酶的微生物，其浓度等于降低的纤维素酶负荷，或者（2）发酵剂已经被工程化以表达细胞结合的纤维素酶，其浓度等于还原的纤维素酶负载在合适的条件下并持续足以允许纤维素底物显着水解的所述时间段。

6. 发明申请

WO2007019505A2 METHOD AND APPARATUS FOR SACCHARIDE PRECIPITATION FROM PRETREATED LIGNOCELLULOSIC MATERIALS 审中-公开
标题翻译：来自预处理的纤维素材料的盐酸盐沉淀的方法和装置
公开(公告)号：WO2007019505A2
公开(公告)日：2007-02-15
申请号：PCT/US2006030894
申请日：2006-08-07
申请人： DARTMOUTH COLLEGE , ZHANG YI-HENG PERCIVAL , LYND LEE R
发明人： ZHANG YI-HENG PERCIVAL , LYND LEE R
IPC分类号： C12P7/08
CPC分类号： C12P19/14 , C12P7/10 , Y02E50/16
摘要： A method for separating saccharide components and lignin fractions from a concentrated acid treated lignocellulosic biomass is disclosed. The method involves precipitating the saccharide components by adding an organic solvent to the biomass slurry. The acid may then be recovered, for example, by filtration or by countercurrent washing and the organic solvent may be flashed and recycled. During acid recovery and organic recovery steps, two main lignocellulose components (hemicellulose and lignin) as well as minor components such as acetic acid are separated as well. The method decreases the amount of cellulase required for hydrolysis, increases hydrolysis rates, reduces formation of inhibitor molecules, increase sugar yields, produces high value by-products such as high quality lignin and hemicellulose, and decreases energy and equipment costs.
摘要翻译：公开了从浓缩酸处理的木质纤维素生物质中分离糖组分和木质素部分的方法。该方法包括通过向生物质浆料中加入有机溶剂来沉淀糖组分。然后可以例如通过过滤或通过逆流洗涤回收酸，并且有机溶剂可以闪蒸并再循环。在酸回收和有机回收步骤期间，两种主要的木质纤维素组分（半纤维素和木质素）以及次要组分如乙酸也被分离。该方法减少了水解所需的纤维素酶的量，增加了水解速率，减少了抑制剂分子的形成，增加了糖的产率，产生了高价值的副产品如高质量的木质素和半纤维素，并降低了能源和设备成本。

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