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1. 发明专利

CA2834865C COMPOSITIONS AND METHODS COMPRISING SERINE PROTEASE VARIANTS 未知
公开(公告)号：CA2834865C
公开(公告)日：2021-03-09
申请号：CA2834865
申请日：2012-05-04
申请人： DANISCO US INC , PROCTER & GAMBLE
发明人： AMIN NEELAM S , AUGUSTYN KATHERINE , BASLER JOSHUA R , CASCAO-PEREIRA LUIS G , COLLIER KATHERINE D , CONCAR EDWARD M , ESTELL DAVID A , KELLIS JAMES T , MAGENNIS EUAN JOHN , PISARCHIK ALEXANDER , POULOSE AYROOKARAN J , SOUTER PHILIP FRANK , WARD GLENN STEVEN , YAO JIAN
IPC分类号： C12N9/54 , C12N15/57
摘要： The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.

2. 发明专利

HUE045202T2 Fogyasztási cikkek proteáz variánsokkal 未知
公开(公告)号：HUE045202T2
公开(公告)日：2019-12-30
申请号：HUE16177383
申请日：2011-05-05
申请人： PROCTER & GAMBLE , DANISCO US INC
发明人： SOUTER PHILIP FRANK , WARD GLENN STEVEN , POULOSE AYROOKARAN JOSEPH , ESTELL DAVID A , KELLIS JR JAMES T , COLLIER KATHERINE D , CASCAO-PEREIRA LUIS GUSTAVO , ALEKSEYEV VIKTOR YURYEVICH , AMIN NEELAM S , YAO JIAN , AUGUSTYN KATHERINE
IPC分类号： C12N9/54 , C11D3/39

3. 发明专利

CA2798451A1 COMPOSITIONS AND METHODS COMPRISING SUBTILISIN VARIANTS 未知
公开(公告)号：CA2798451A1
公开(公告)日：2011-11-10
申请号：CA2798451
申请日：2011-05-05
申请人： DANISCO US INC , PROCTER & GAMBLE
发明人： ALEKSEYEV VIKTOR YURYEVICH , AMIN NEELAM S , AUGUSTYN KATHERINE , CASCAO-PEREIRA LUIS GUSTAVO , COLLIER KATHERINE D , ESTELL DAVID A , KELLIS JAMES T JR , POULOSE AYROOKARAN J , SOUTER PHILIP FRANK , WARD GLENN STEVEN , YAO JIAN
IPC分类号： C12N9/54 , C11D3/386 , C11D3/39
摘要： The present invention provides serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.

4. 发明专利

ES2745011T3 Productos de consumo con variantes de proteasa 未知
公开(公告)号：ES2745011T3
公开(公告)日：2020-02-27
申请号：ES16177383
申请日：2011-05-05
申请人： PROCTER & GAMBLE , DANISCO US INC
发明人： SOUTER PHILIP FRANK , WARD GLENN STEVEN , POULOSE AYROOKARAN JOSEPH , ESTELL DAVID A , KELLIS JR JAMES T , COLLIER KATHERINE D , CASCAO-PEREIRA LUIS GUSTAVO , ALEKSEYEV VIKTOR YURYEVICH , AMIN NEELAM S , YAO JIAN , AUGUSTYN KATHERINE
IPC分类号： C12N9/54 , C11D3/386 , C11D3/39
摘要： Una composición que comprende un material adyuvante y una variante de proteasa de agua fría; siendo dicha composición un producto de consumo, y siendo dicha variante de proteasa una variante de una proteasa precursora; siendo dicha secuencia de proteasa precursora, al menos, un 95 % idéntica a la secuencia de aminoácidos id. de sec. n.° 1, teniendo dicha variante una o más de las siguientes características: a) un índice de rendimiento del Método de ensayo 2 de al menos 1,1, al menos 1,2, al menos 1,3, al menos 1,4, al menos 1,5, al menos 1,6, al menos 1,7, al menos 1,8, al menos 1,9, al menos 2; de 1,1 a 10, de 1,1 a 8 o incluso de 1,1 a 5; b) un índice de rendimiento del Método de ensayo 3 de al menos 1,1, al menos 1,2, al menos 1,3, al menos 1,4, al menos 1,5, al menos 1,6, al menos 1,7, al menos 1,8, al menos 1,9, al menos 2; de 1,1 a 10, de 1,1 a 8 o incluso de 1,1 a 5; c) un índice de rendimiento del Método de ensayo 4 de al menos 1,0, al menos 1,1, al menos 1,2, al menos 1,3, al menos 1,4, al menos 1,5, al menos 1,6, al menos 1,7, al menos 1,8, al menos 1,9, al menos 2, de 1,0 a 10, de 1,0 a 8 o incluso de 1,0 a 5; d)un índice de rendimiento del Método de ensayo 6 de al menos 1,1, al menos 1,2, al menos 1,3, al menos 1,4, al menos 1,5, al menos 1,6, al menos 1,7, al menos 1,8, al menos 1,9, al menos 2; de 1,1 a 10, de 1,1 a 8 o incluso de 1,1 a 5, en donde el Método de ensayo 2, el Método de ensayo 3, el Método de ensayo 4 y el Método de ensayo 6 se describen explícitamente en los "MÉTODOS DE ENSAYO" de la presente memoria, siendo dicha variante de proteasa mutada de tal manera que las mutaciones consisten de uno de los siguientes conjuntos de mutaciones, en donde cada posición de aminoácido está numerada según la numeración de la posición de aminoácido correspondiente en la secuencia de aminoácidos de la subtilisina BPN' de Bacillus amyloliquefaciens mostrada en la id. de sec. n.° 2:**Tabla**

5. 发明专利

BR112012028466A2 produtos destinados ao consumidor com variantes de protease 未知
公开(公告)号：BR112012028466A2
公开(公告)日：2015-09-22
申请号：BR112012028466
申请日：2011-05-05
申请人： DANISCO US INC , PROCTER & GAMBLE
发明人： POULOSE AYROOKARAN JOSEPH , ESTELL DAVID A , WARD GLENN STEVEN , KELLIS JAMES T JR , YAO JIAN , AUGUSTYN KATHERINE , COLLIER KATHERINE D , CASCÃO-PEREIRA LUIS GUSTAVO , AMIN NEELAM S , SOUTER PHILIP FRANK , ALEKSEYEV VIKTOR YURYEVICH
IPC分类号： C11D3/386 , C11D3/39 , C12N9/54
摘要： produtos destinados ao consumidor com variantes de protease. a presente invenção refere-se a produtos destinados ao consumidor que compreendem proteases, particularmente, proteases de água fria e processos para produzir e usar tais produtos. tais produtos destinados ao consumidor fornecem limpeza, brancura e/ou frescor otimizado. tais proteases são derivadas de uma enzima parental, por exemplo, bacillus lentus, por substituição, inserção e/ou deleção de uma ou mais dos aminoácidos das enzimas parentais.

6. 发明专利

CA2834865A1 COMPOSITIONS AND METHODS COMPRISING SERINE PROTEASE VARIANTS 未知
公开(公告)号：CA2834865A1
公开(公告)日：2012-11-08
申请号：CA2834865
申请日：2012-05-04
申请人： DANISCO US INC , PROCTER & GAMBLE
发明人： AMIN NEELAM S , AUGUSTYN KATHERINE , BASLER JOSHUA R , CASCAO-PEREIRA LUIS G , COLLIER KATHERINE D , CONCAR EDWARD M , ESTELL DAVID A , KELLIS JAMES T JR , MAGENNIS EUAN JOHN , PISARCHIK ALEXANDER , POULOSE AYROOKARAN J , SOUTER PHILIP FRANK , WARD GLENN STEVEN , YAO JIAN
IPC分类号： C12N9/54 , C12N15/57
摘要： The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.

7. 发明专利

CA2798451C COMPOSITIONS AND METHODS COMPRISING SUBTILISIN VARIANTS WITH T022A-E271F SUBSTITUTIONS 未知
公开(公告)号：CA2798451C
公开(公告)日：2020-10-06
申请号：CA2798451
申请日：2011-05-05
申请人： DANISCO US INC , PROCTER & GAMBLE
发明人： ALEKSEYEV VIKTOR YURYEVICH , AMIN NEELAM S , AUGUSTYN KATHERINE , CASCAO-PEREIRA LUIS GUSTAVO , COLLIER KATHERINE D , ESTELL DAVID A , KELLIS JAMES T , POULOSE AYROOKARAN J , SOUTER PHILIP FRANK , WARD GLENN STEVEN , YAO JIAN
IPC分类号： C12N9/54 , C11D3/386 , C11D3/39
摘要： The present invention provides serine protease variants having amino acid substitutions relative to a Bacillus lentus subtilisin GG36 protease as well as associated nucleic acids, cells and methods. One serine protease variant that is provided has sequence identity to a Bacillus lentus subtilisin protease and T022A-E271F substitutions. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.

8. 发明专利

ES2694398T3 Composiciones y métodos que comprenden variantes de subtilisina 未知
公开(公告)号：ES2694398T3
公开(公告)日：2018-12-20
申请号：ES11730124
申请日：2011-05-05
申请人： DANISCO US INC , PROCTER & GAMBLE
发明人： ALEKSEYEV VIKTOR YURYEVICH , AMIN NEELAM , AUGUSTYN KATHERINE , CASCAO-PEREIRA LUIS GUSTAVO , COLLIER KATHERINE , ESTELL DAVID , KELLIS JR , POULOSE AYROOKARAN , SOUTER PHILIP FRANK , WARD GLENN STEVEN , YAO JIAN
IPC分类号： C11D3/386 , C12N9/54
摘要： Variante de subtilisina aislada, donde dicha variante de subtilisina es una forma madura que presenta actividad proteolítica y comprende una secuencia de aminoácidos comprendiendo una combinación de sustituciones de aminoácidos seleccionadas de entre: G020R-N043R-A230E, G020R-N043R-A230E-S242R, G020R-N043RE271L, G020R-N043R-H249R, G020R-N043R-H249R-E271L, G020R-N043R-N076D, G020R-N043R-N076D-A230E-H249R, G020R-N043R-N076D-S101G-S103A-V104I-A232V-Q245R-N269R, , G020R-N043R-N269R, G020R-N043R-R045T-A230E, , G020R-N043R-R045T-S101A-N269R, G020R-N043R-R045T-S242R, G020R-N043R-S101A-N116A-T213A-A215F, G020R-N043R-S101A-N269R, G020R-N043R-S101G-S103A-V104I-A232V-Q245R, , G020R-N043R-S212F, G020R-N043R-S212F-W241R, G020R-N043R-S242R, G020R-N043R-S242R-E271L, G020R-N043R-V244R, G020R-N043R-W241R, G020R-S024R-N043R-N076D, G020R-S024R-N043R-R045T, G020R-S024R-N043R-R045T-A215F, G020R-S024R-N043R-R045T-H249R, G020R-S024R-N043R-R045T-N076D-S242R-H249R, G020R-S024R-N043R-R045T-N116A, G020R-S024R-N043R-R045T-N116A-T213A, G020R-S024R-N043R-R045T-N116A-T213A-A215F, G020R-S024R-N043R-R045T-S101A-N269R, G020R-S024R-N043R-R045T-S101A-T213A,, G020R-S024R-N043R-S242R, G020R-S024R-T38I-N043R-R045T-N076D-S242R-H249R, G020R-T022R-N043R, G020R-T022R-N043R-S212F, G020R-T022R-N043R-W241R, G020R-T038A-N043R-S101A, N018R-G020R-N043R, N018R-G020R-N043R-N076D, N018R-G020R-N043R-N076D-A230E-S242R, N018R-G020R-N043R-N076D-H249R, N018R-G020R-N043R-N076D-S242R-H249R, N018R-G020R-N043R-R045T-N076D-H249R, N018R-G020R-N043R-R045T-N076D-S101G-S103A-V104I-A232V-Q245R, N018R-G020R-N043R-R045T-S242R, N018R-G020R-S024R-N043R-N076D-H249R, N018R-G020R-S024R-N043R-R045T-N076D-A230E, N018R-G020R-S024R-N043R-R045T-N076D-H249R, P014L-G020R-S024R-N043R-R045T-S101A-A215F, S009A-G020R-N043R-S212F, S009A-G020R-N043R-W241R, S009A-G020R-S024R-N043R, V004R-G020R-N043R, V004R-G020R-S024R-N043R, V004R-G020R-S024R-N043R-S242R, V004R-S009A-G020R-N043R y V004R-S009A-G020R-N043R-S242R, donde la carga neta total de la variante es 0, +1, +2, +3, +4, +5, -1, -2, -3, -4, o -5 en relación con la carga neta total de la proteasa subtilisina GG36 de Bacillus lentus, y donde la carga neta total se obtiene mediante una o más sustituciones seleccionada(s) de entre: R45T, N62E, N76D, S101D, P129E, A158E, G159D, G159E, S166D, S188D, A230E, N18R, T22R, S24R, G118R, Q245R, H249R, N269R, E271F y E271L, donde la variante es una variante de una proteasa parental proteasa subtilisina GG36 de Bacillus lentus comprendiendo la secuencia de aminoácidos mostrada en la SEQ ID N.º 2, donde dicha variante presenta al menos un 90 % de identidad de secuencia con respecto a la secuencia de aminoácidos de la SEQ ID N.º 2, y donde las posiciones de aminoácidos de la variante de proteasa se numeran conforme a la numeración de posiciones de aminoácidos correspondientes en la secuencia de aminoácidos de subtilisina BPN' de Bacillus amyloliquefaciens que se muestra en la SEQ ID N.º 1.

9. 发明专利

BR112012028467A2 composições e métodos que compreendem variantes de subtilisina 未知
公开(公告)号：BR112012028467A2
公开(公告)日：2015-09-22
申请号：BR112012028467
申请日：2011-05-05
申请人： DANISCO US INC , PROCTER & GAMBLE
发明人： POULOSE AYROOKARAN J , ESTELL DAVID A , WARD GLENN STEVEN , KELLIS JAMES T JR , YAO JIAN , AUGUSTYN KATHERINE , COLLIER KATHERINE D , CASCAO-PEREIRA LUIS GUSTAVO , AMIN NEELAM S , SOUTER PHILIP FRANK , ALEKSEYEV VIKTOR YURYEVICH
IPC分类号： C11D3/386 , C11D3/39 , C12N9/54
摘要： composições e métodos que compreendem variantes de subtilisina. a presente invenção apresenta variantes de protease de serina produzidas a partir da invenção. especificamente, a presente invenção fornece variantes de serina protease que possuem uma ou mais substituições em comparação a uma serina protease de referência. além disso, a presente invenção apresenta composições que compreendem estas variantes de serina protease. em algumas modalidades, a presente invenção apresenta composições de limpeza que compreendem pelo menos uma destas variantes de serina protease.

10. 发明专利

CA3111256A1 COMPOSITIONS AND METHODS COMPRISING SERINE PROTEASE VARIANTS 未知
公开(公告)号：CA3111256A1
公开(公告)日：2012-11-08
申请号：CA3111256
申请日：2012-05-04
申请人： DANISCO US INC , PROCTER & GAMBLE
发明人： AMIN NEELAM S , AUGUSTYN KATHERINE , BASLER JOSHUA R , CASCAO-PEREIRA LUIS G , COLLIER KATHERINE D , CONCAR EDWARD M , ESTELL DAVID A , KELLIS JAMES T , MAGENNIS EUAN JOHN , PISARCHIK ALEXANDER , POULOSE AYROOKARAN J , SOUTER PHILIP FRANK , WARD GLENN STEVEN , YAO JIAN
摘要： The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.

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