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1. 发明申请

WO1992020813A1 ASSAYS FOR FACTORS AFFECTING CIRCULARIZATION OF DNA, ASSAYS FOR FACTORS AFFECTING DNA INTEGRATION, FACTORS, AND USES THEREOF 审中-公开
标题翻译：测定影响DNA循环的因子，影响DNA整合因素及其用途的因素测定
公开(公告)号：WO1992020813A1
公开(公告)日：1992-11-26
申请号：PCT/US1992004136
申请日：1992-05-15
申请人： DANA FARBER CANCER INSTITUTE
发明人： DANA FARBER CANCER INSTITUTE , HASELTINE, William, A. , FARNET, Christopher, M.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6897
摘要： An assay for factors that affect integration of DNA into target DNA is disclosed. Assays for methods of screening for factors which affect viral DNA circularization either by homologous recombination, end-to-end ligation, or autointegration, are also disclosed. A method for screening for factors which will enhance circularization rather than integration by testing cellular cytoplasmic fluid under conditions which permit circularization in the fluid is also described. Factors which affect integration and circularization are disclosed. Therapeutic methods for retarding viral infection are also described.
摘要翻译：公开了影响DNA整合到目标DNA中的因素的测定。还公开了通过同源重组，端对端连接或自动整合来筛选影响病毒DNA环化的因子的方法的测定。还描述了在允许在流体中进行环化的条件下筛选能够增强环化而不是通过测试细胞质胞浆的整合的因素的方法。披露影响整合和循环的因素。还描述了用于延缓病毒感染的治疗方法。

2. 发明申请

WO1991019798A1 VECTORS CONTAINING HIV PACKAGING SEQUENCES, PACKAGING DEFECTIVE HIV VECTORS, AND USES THEREOF 审中-公开
标题翻译：包含HIV包装序列的载体，包装有缺陷的HIV载体及其用途
公开(公告)号：WO1991019798A1
公开(公告)日：1991-12-26
申请号：PCT/US1991004335
申请日：1991-06-18
申请人： DANA FARBER CANCER INSTITUTE
发明人： DANA FARBER CANCER INSTITUTE , SODROSKI, Joseph, G. , HASELTINE, William, A. , POZNANSKY, Mark , LEVER, Andrew
IPC分类号： C12N15/00
CPC分类号： C12N15/86 , A61K38/00 , A61K39/00 , C07K14/005 , C12N2740/16043 , C12N2740/16052 , C12N2740/16122
摘要： Packaging defective and packaging proficient HIV vectors are disclosed. These vectors can be used to establish HIV packaging defective cell lines, and to package desired genes. These cell lines can be used in developing a vaccine, HIV antibodies and as part of a system for gene transfer. The packaging proficient vector can be used to target HIV target cells.
摘要翻译：公开了包装缺陷和包装精通HIV载体。这些载体可用于建立HIV包装有缺陷的细胞系，并包装所需的基因。这些细胞系可用于开发疫苗，HIV抗体以及作为基因转移系统的一部分。包装精通载体可用于靶向HIV靶细胞。

3. 发明申请

WO1990015149A1 VPT PROTEIN, VECTORS EXPRESSING THIS PROTEIN, CELL PRODUCTS AND ASSAY METHODS 审中-公开
标题翻译： VPT蛋白，表达本蛋白，细胞产物和测定方法的载体
公开(公告)号：WO1990015149A1
公开(公告)日：1990-12-13
申请号：PCT/US1990003123
申请日：1990-06-01
申请人： DANA FARBER CANCER INSTITUTE
发明人： DANA FARBER CANCER INSTITUTE , HASELTINE, William, A. , COHEN, Eric
IPC分类号： C12P21/00
CPC分类号： C07K14/005 , A61K38/00 , A61K39/00 , C07K16/1072 , C12N7/00 , C12N2740/10022 , C12N2740/16021 , C12N2740/16322 , Y10S435/974 , Y10S530/826 , Y10S930/221
摘要： A protein having a molecular weight of approximately 17kD is disclosed. This protein is referred to as viral protein T and produced by the vpt gene. This protein has antigenic determinants.
摘要翻译：公开了分子量约17kD的蛋白质。该蛋白质称为病毒蛋白T，由vpt基因产生。该蛋白具有抗原决定簇。

4. 发明申请

WO1990004020A1 H. SAIMIRI-HTLV-X REGION VECTOR 审中-公开
标题翻译： H. SAIMIRI-HTLV-X区域向量
公开(公告)号：WO1990004020A1
公开(公告)日：1990-04-19
申请号：PCT/US1989004308
申请日：1989-10-02
申请人： DANA FARBER CANCER INSTITUTE , BEHRINGWERKE AKTIENGESELLSCHAFT
发明人： DANA FARBER CANCER INSTITUTE , BEHRINGWERKE AKTIENGESELLSCHAFT , HASELTINE, William, A. , MCGUIRE, Kathleen , DOKHELAR, Marie-Christine , GRASSMANN, Ralph , FLECKENSTEIN, Bernhard , MÜLLER-FLECKENSTEIN, Ingrid
IPC分类号： C12N07/00
CPC分类号： C12N15/86 , C12N2710/16443 , C12N2740/14022 , C12N2830/60
摘要： A H. saimiri-HTLV-1 or 2 X region vector is disclosed. This vector can be used to establish continuous cell lines of difficult to grow cells, such as human T-cells. It can also be used to obtain certain cell products and in methods for screening new compounds.
摘要翻译：公开了一种H.赛默里-HTLV-1或2X区载体。该载体可用于建立难以生长的细胞如人T细胞的连续细胞系。它也可用于获得某些细胞产物和筛选新化合物的方法。

5. 发明申请

WO1991018093A1 A SUBPACKAGEABLE VECTOR FOR TRANSPOSABLE ELEMENTS 审中-公开
标题翻译：用于可移动元件的可拆卸矢量
公开(公告)号：WO1991018093A1
公开(公告)日：1991-11-28
申请号：PCT/US1991003605
申请日：1991-05-22
申请人： DANA FARBER CANCER INSTITUTE
发明人： DANA FARBER CANCER INSTITUTE , HASELTINE, William, A. , MYRICK, Kyl, V. , FARNET, Christopher, M.
IPC分类号： C12N15/00
CPC分类号： C12N15/70 , C12N15/10
摘要： Undersized monomeric vectors, undersized and concatermerizable vector units and vectors containing a plurality of the undersized vector units are disclosed. These vectors can be used for primary cloning, subcloning and analyzing transposable elements.
摘要翻译：公开了不足的单体载体，不足够和可连接的载体单元和含有多个尺寸过小的载体单元的载体。这些载体可用于初级克隆，亚克隆和分析转座元件。

6. 发明申请

WO1987007296A1 EXPRESSION OF HUMAN T-CELL LYMPHOTROPIC VIRUS (HTLV-III) REVERSE TRANSCRIPTASE AND USES THEREOF 审中-公开
标题翻译：人类T细胞淋巴瘤病毒（HTLV-III）逆转录酶的表达及其用途
公开(公告)号：WO1987007296A1
公开(公告)日：1987-12-03
申请号：PCT/US1987001162
申请日：1987-05-20
申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY ... , THE DANA-FARBER CANCER INSTITUTE
发明人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY ... , THE DANA-FARBER CANCER INSTITUTE , GOFF, Stephen, P. , HASELTINE, William, A. , TANESE, Naoko
IPC分类号： C12N01/00
CPC分类号： C07K14/005 , C12N9/1276 , C12N15/71 , C12N2740/16222 , C12Q1/703
摘要： A plasmid which, when introduced into a suitable host cell and grown under appropriate conditions, effects expression of a gene on the plasmid and production of a polypeptide having HTLV-III reverse transcriptase activity. The plasmid is a double-stranded DNA molecule which includes in a 5' to 3' order the following: a DNA sequence which includes an inducible promoter; a DNA sequence which includes an ATG initiation codon; a portion of the Human T-cell lymphotropic virus III (HTLV-III) pol gene, said portion including a DNA sequence which encodes the polypeptide having HTLV-III reverse transcriptase activity; a DNA sequence which contains a gene associated with a selectable or identifiable phenotypic trait which is manifested when the vector is present in the host cell; and a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell. The invention also provides a method for identifying substances which inhibit HTLV-III reverse transcriptase and a method for treating an HTLV-III virus-related disease.
摘要翻译：一种质粒，当被引入合适的宿主细胞并在合适的条件下生长时，影响基因在质粒上的表达并产生具有HTLV-III逆转录酶活性的多肽。质粒是双链DNA分子，其以5'至3'的顺序包括以下：包含诱导型启动子的DNA序列; 包括ATG起始密码子的DNA序列; 人类T细胞淋巴细胞病毒III（HTLV-III）pol基因的一部分，所述部分包括编码具有HTLV-III逆转录酶活性的多肽的DNA序列; 包含与可选择或可鉴定的表型性状相关的基因的DNA序列，其在载体存在于宿主细胞中时表现出来; 以及含有能够在宿主细胞中自主复制的细菌质粒的复制起点的DNA序列。本发明还提供了用于鉴定抑制HTLV-III逆转录酶的物质的方法和用于治疗HTLV-III病毒相关疾病的方法。

7. 发明申请

WO1985005636A1 TRANS-ACTING TRANSCRIPTIONAL FACTORS 审中-公开
标题翻译：转基因转录因子
公开(公告)号：WO1985005636A1
公开(公告)日：1985-12-19
申请号：PCT/US1985000985
申请日：1985-05-24
申请人： DANA-FARBER CANCER INSTITUTE
发明人： DANA-FARBER CANCER INSTITUTE , HASELTINE, William, A. , SODROSKI, Joseph, G. , ROSEN, Craig, A.
IPC分类号： C12P19/34
CPC分类号： C07K14/005 , C12N15/85 , C12N2740/14022 , C12N2740/16322
摘要： Products and methods for the expression of gene products by recombinant DNA techniques. The invention relates to the discovery of a novel phenomenon in retrovirus gene regulation, namely trans-activation. Described herein are novel trans-acting factors which may be employed to enhance the production of homologous or heterologous genes. The trans-acting regulatory genes of HTLV-I, HTLV-II, HTLV-III and BLV are disclosed. Also disclosed are cis-acting regulatory elements within the viral LTR sequences that respond to the trans-acting viral factors. The cis-acting regulatory elements (called tar elements for the trans-activation responsive) of the genomes of HTLV-I, HTLV-II, HTLV-III and BLV are described. Paired expression systems that include a trans-acting factor derived from trans-activating retrovirus, paired with an expression vector which includes responsive cis-acting viral factors. The cis-acting regulatory elements 5' to the gene to be expressed, provide substantially increased expression of gene products in a variety of animal cells. Suitable hosts include human lymphoid cells, human fibroblasts, human epithelial cells, rodent cell lines, e.g. murine lymphocite, murine fibroblast and Chinese hamster ovary cells, and feline and simian epithelial cells.
摘要翻译：通过重组DNA技术表达基因产物的产物和方法。本发明涉及逆转录病毒基因调控中的新现象的发现，即反式激活。本文描述的是可用于增强同源或异源基因产生的新的反式作用因子。公开了HTLV-I，HTLV-II，HTLV-III和BLV的反式调节基因。还公开了响应于反作用病毒因子的病毒LTR序列内的顺式作用调节元件。描述了HTLV-1，HTLV-II，HTLV-III和BLV的基因组的顺式作用调节元件（称为反式激活反应的焦油元件）。配对表达系统，其包括衍生自反式激活逆转录病毒的反式作用因子，与包括响应性顺式作用病毒因子的表达载体配对。要表达的基因的顺式作用调节元件5'提供基因产物在多种动物细胞中的显着增加的表达。合适的宿主包括人淋巴细胞，人成纤维细胞，人上皮细胞，啮齿动物细胞系，鼠淋巴细胞，鼠成纤维细胞和中国仓鼠卵巢细胞，以及猫和猿上皮细胞。

8. 发明申请

WO1985005629A1 LTR VECTORS, METHODS OF PREPARATION AND USE 审中-公开
标题翻译： LTR VECTORS，制备和使用方法
公开(公告)号：WO1985005629A1
公开(公告)日：1985-12-19
申请号：PCT/US1985000986
申请日：1985-05-24
申请人： DANA-FARBER CANCER INSTITUTE
发明人： DANA-FARBER CANCER INSTITUTE , HASELTINE, William, A. , ROSEN, Craig, A. , LENZ, Jack, R. , CELANDER, Daniel, W.
IPC分类号： C12N01/00
CPC分类号： C12N15/85
摘要： Process for the tissue specific expression of heterologous genes using modified LTR vectors. Any retroviral LTR can be synthetically modified in its enhancer region using the methods described herein and tissue specificity can thereby be achieved. Also described is an assay useful in proving or predicting tissue specificity of the modified LTR vector. This invention is also directed to novel tissue specific LTR vectors. Long terminal repeats (LTRs) of Moloney sacoma virus (MSV) have been shown useful in activating the expression of a normally quiescent cellular DNA sequence by Vande Woude et al., in U.S. Patent No. 4,405,712. The present invention demonstrates the use of modified mammalian LTR vectors for obtaining selectable tissue expression of heterologous genes.
摘要翻译：使用修饰的LTR载体的异源基因的组织特异性表达的方法。任何逆转录病毒LTR可以使用本文所述的方法在其增强子区域进行合成修饰，从而可以实现组织特异性。还描述了可用于证实或预测修饰的LTR载体的组织特异性的测定法。本发明还涉及新的组织特异性LTR载体。已经显示，Moloney sacoma病毒（MSV）的长末端重复序列（LTR）可用于Vande Woude等在美国专利号4,405,712中激活正常静止细胞DNA序列的表达。本发明证明使用修饰的哺乳动物LTR载体来获得异源基因的可选择的组织表达。

9. 发明申请

WO1993012232A1 REACTIVE NEUTRALIZING HUMAN ANTI-GP120 RECOMBINANT ANTIBODY, DNA CODING THE SAME AND USE THEREOF 审中-公开
标题翻译：反应性中和人抗GP21重组抗体，编码其的DNA及其用途
公开(公告)号：WO1993012232A1
公开(公告)日：1993-06-24
申请号：PCT/US1992010928
申请日：1992-12-10
申请人： DANA FARBER CANCER INSTITUTE , NEW ENGLAND DEACONNESS HOSPITAL CORPORATION
发明人： DANA FARBER CANCER INSTITUTE , NEW ENGLAND DEACONNESS HOSPITAL CORPORATION , MARASCO, Wayne, A. , SODROSKI, Joseph, G. , POSNER, Marshall, R. , HASELTINE, William, A.
IPC分类号： C12N15/13
CPC分类号： C07K16/1063 , A61K38/00
摘要： The present invention is directed to a recombinant human monoclonal antibody which binds to a discontinuous epitope on the HIV gp120 envelope glycoprotein, blocks the binding of gp120 to the CD4 receptor, and neutralizes a broad range of HIV isolates. The present invention also provides the primary nucleotide and deduced amino acid sequences of the rearranged heavy and light chains of the recombinant monoclonal antibody of the present invention, and a method of screening for antibodies which block binding of envelope glycoprotein to the CD4 receptor.
摘要翻译：本发明涉及与HIV gp120包膜糖蛋白上的不连续表位结合的重组人单克隆抗体，阻断gp120与CD4受体的结合，并中和大范围的HIV分离物。本发明还提供本发明的重组单克隆抗体的重排重链和轻链的主要核苷酸和推导的氨基酸序列，以及筛选封闭糖蛋白与CD4受体结合的抗体的方法。

10. 发明申请

WO1991019817A1 EXPRESSION VECTORS CONTAINING THE HIV nef GENE TO ENHANCE REPLICATION EFFICIENCY 审中-公开
标题翻译：含有HIV nef基因的表达载体提高复制效率
公开(公告)号：WO1991019817A1
公开(公告)日：1991-12-26
申请号：PCT/US1991004333
申请日：1991-06-18
申请人： DANA FARBER CANCER INSTITUTE
发明人： DANA FARBER CANCER INSTITUTE , HASELTINE, William, A. , TERWILLIGER, Ernest , LANGHOF, Erik
IPC分类号： C12Q01/68
CPC分类号： C07K14/005 , C12N2740/16322
摘要： Vectors containing a sufficient number of nucleotides to express HIV gene products necessary to be replication-competent and also containing an ELI nef gene segment are disclosed. These vectors can be used to transfect a wide range of cells and hosts for use in assay systems.
摘要翻译：公开了含有足够数目的核苷酸以表达需要具有复制能力并且还含有ELI nef基因片段的HIV基因产物的载体。这些载体可用于转染用于测定系统的各种细胞和宿主。

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