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1. 发明专利

JPS62285781A CULTURE OF CELL 失效
公开(公告)号：JPS62285781A
公开(公告)日：1987-12-11
申请号：JP12941986
申请日：1986-06-04
申请人： DAIICHI SEIYAKU CO , KOKEN KK
发明人： YOSHIZATO KATSUTOSHI , SASAKI KENICHI , YAMAMOTO NOBORU , TAKAYAMA SATOSHI , MIYATA TERUO
IPC分类号： C12N5/00 , C12N5/07 , C12N5/071 , C12R1/91
摘要： PURPOSE:To enable the culture and maintenance of hepatic cell over a long period, by planting hepatic cell to a feeder layer of a fibroblast cell which is terminated its proliferation capability by X-ray irradiation, or by planting fibroblast cell under a porous thin film and planting hepatic cell to the opposite side of the film. CONSTITUTION:3T3 terminated its proliferation capability by the X-ray irradiation with conventional X-ray radiation apparatus at a dose of 3,000-10,000 rad, fibroblast cell originated from liver, fibroblast cell originated from skin, etc., is used as a feeder layer. Hepatic cell originated from mammal is subjected, immediately after separation, to stacking culture on the feeder layer to enable the long-term maintenance of hepatic cell. As an alternative method, a collagen solution is formed in the form of a sheet, fibrillated and crosslinked by UV irradiation to obtain active collagen thin film. A fibroblast cell is planted under the collagen film and a hepatic cell is planted to the reverse side of the film. The cell can be cultured over a long period in a culture liquid such as Green medium. It is also possible to exclusively separate and recover the hepatic cell.

2. 发明专利

JPS6242892B2 失效
公开(公告)号：JPS6242892B2
公开(公告)日：1987-09-10
申请号：JP8000378
申请日：1978-06-30
申请人： DAIICHI SEIYAKU CO
发明人： TAKAYAMA SATOSHI , FURUHAMA KAZUHISA
IPC分类号： A61K49/00

3. 发明专利

JPS557245A COLORANT FOR LYMPH SYSTEM 失效
公开(公告)号：JPS557245A
公开(公告)日：1980-01-19
申请号：JP8000378
申请日：1978-06-30
申请人： DAIICHI SEIYAKU CO
发明人： TAKAYAMA SATOSHI , FURUHAMA KAZUHISA
IPC分类号： A61K49/00
摘要： PURPOSE:A colorant for the lymph system having improved coloring effect and safety, comprising Indocyanine Green. CONSTITUTION:Indocyamine Green or its aqueous solution is used as a lymphatic colorant in a dose of preferably 1 ml based on 1 a 0.5% aqueous solution. The colorant is preferably injected into the peripheral subcutaneous site, into the muscle or under the serous memberane for constrast media or coloring of the lymph system in operation of cancer. The colorant is better than the conventional Evans blue.

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