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    • 4. 发明授权
    • Pufa polyketide synthase systems and uses thereof
    • Pufa聚酮化合物系统及其用途
    • US07803620B2
    • 2010-09-28
    • US11689453
    • 2007-03-21
    • Craig A. WeaverRoss ZirkleJames G. Metz
    • Craig A. WeaverRoss ZirkleJames G. Metz
    • C12N15/82C12N1/20C07H21/04
    • C12P7/6472C07K14/195C12N9/001C12N9/1029C12N15/52C12N15/8247C12P7/62C12P7/6427
    • Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.
    • 公开了来自细菌微生物Shewanella japonica和Shewanella olleyana的完全多不饱和脂肪酸(PUFA)聚酮化合物(PKS)系统及其生物活性片段和同系物。 更具体地,本发明涉及编码这种PUFA PKS系统的核酸,其包含这种PUFA PKS系统的蛋白质和结构域到包含这种PUFA PKS系统的转基因生物(植物和微生物),以及制备和使用 本文公开的PUFA PKS系统。 本发明还涉及基因修饰的植物和微生物以及通过操作PUFA聚酮化合物合酶(PKS)系统来有效地产生富含多种不饱和脂肪酸(PUFA)以及其它生物活性分子的脂质的方法。