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1. 发明申请

US20090209485A1 MODULATING ANGIOGENESIS WITH NOD FACTORS SUCH AS GLUCOSAMINE OLIGOSACCHARIDES 审中-公开
标题翻译：用NOD因子调节血管发生，如葡萄糖胺低聚糖
公开(公告)号：US20090209485A1
公开(公告)日：2009-08-20
申请号：US11910166
申请日：2006-05-31
申请人： Christopher Richard Parish , Michael A. Djordjevic , Barry G. Rolfe , Peter M. Gresshoff
发明人： Christopher Richard Parish , Michael A. Djordjevic , Barry G. Rolfe , Peter M. Gresshoff
IPC分类号： A61K31/715 , A61P35/00 , A61P35/04
CPC分类号： A61K31/7016 , A61K31/702 , A61K31/7024 , A61K31/715
摘要： This invention relates to the use of Nod factors and derivatives thereof for the modulation of blood vessel growth and development as well as compositions for modulating angiogenesis.
摘要翻译：本发明涉及节点因子及其衍生物用于调节血管生长和发育以及用于调节血管发生的组合物的用途。

2. 发明授权

US5492810A DNA silver staining 失效
标题翻译： DNA银染
公开(公告)号：US5492810A
公开(公告)日：1996-02-20
申请号：US193514
申请日：1994-02-08
申请人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
发明人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
IPC分类号： G01N27/447 , C12Q1/68 , G01N27/26
CPC分类号： G01N27/44726
摘要： A method for visualizing nucleic acids in a polyacrylamide gel. The method comprises fixing the nucleic acids with 10% acetic acid for about 20 minutes, washing the gel multiple times with water for about 2 minutes, impregnating the gel with silver nitrate at a concentration of about 1.0 g/l and about 1.5 ml/l of 37% formaldehyde for about 30 minutes, developing the gel with sodium carbonate at a concentration of about 30 g/l, 1.5 ml/l of 37% formaldehyde and sodium thiosulfate pentahydrate at a concentration of about 2.0 mg/l for between about 2 minutes and about 5 minutes, and then stopping the development of the gel by treatment with 10% acetic acid for about 5 minutes.
摘要翻译：一种在聚丙烯酰胺凝胶中可视化核酸的方法。该方法包括用10％乙酸固定核酸约20分钟，用水洗涤凝胶多次约2分钟，用浓度约1.0g / l和约1.5ml / l的硝酸银浸渍凝胶的37％甲醛约30分钟，用约30g / l的浓度的碳酸钠，1.5ml / l的37％甲醛和硫代硫酸钠五水合物以约2.0mg / l的浓度显影凝胶约2 分钟和约5分钟，然后用10％乙酸处理约5分钟停止凝胶的显影。

3. 发明授权

US5124504A Nitrate-tolerant soybean 失效
标题翻译：耐硝酸的大豆
公开(公告)号：US5124504A
公开(公告)日：1992-06-23
申请号：US16274
申请日：1987-02-19
申请人： Peter M. Gresshoff , Bernard J. Carroll , David L. McNeil
发明人： Peter M. Gresshoff , Bernard J. Carroll , David L. McNeil
IPC分类号： A01H1/02 , A01H5/10 , C12N15/01
CPC分类号： C12N15/01 , A01H1/02 , A01H5/10
摘要： Genetically manipulated soybeam varieties which display nitrate-tolerant nodulation and supernodulation are described. The soybean nodulation mutants of the present invention can be specifically prepared by induced mutagenesis followed by appropriate phenotype screening. Fifteen individual mutants having nitrate tolerant nodulation and/or supernodulation phenotypes have been isolated. Soybean varieties having the nodulation phenotypes of the present invention are useful in the development of novel and agronomically important varieties of soybean.
摘要翻译：描述了显示硝酸盐耐受性结瘤和超调的遗传操纵大豆品种。本发明的大豆结瘤突变体可以通过诱导诱变，然后进行适当的表型筛选来特异性地制备。已经分离了具有耐硝酸盐结瘤和/或超调节表型的十五个个体突变体。具有本发明的结瘤表型的大豆品种可用于开发新的和农业上重要的大豆品种。

4. 发明申请

US20140053294A1 Pongamia Genetic Markers and Method of Use 审中-公开
标题翻译： Pongamia遗传标记和使用方法
公开(公告)号：US20140053294A1
公开(公告)日：2014-02-20
申请号：US13885886
申请日：2011-11-18
申请人： Peter M. Gresshoff
发明人： Peter M. Gresshoff
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6895 , C07H21/04 , C12Q2600/13 , C12Q2600/156
摘要： Primers suitable for nucleic acid sequence amplification of Pongamia plant genetic markers and a method of genetic analysis of Pongamia plants are provided. The primers comprise a repeat unit of two or three nucleotides repeated five to ten times together with a three prime extension of two or three nucleotides. Genetic markers amplified by the primers are also provided, from which may be produced further primers for genetic analysis of Pongamia plants. The primers, genetic markers and methods of genetic analysis may be suitable for selection and breeding of Pongamia plants having desired traits such as, or relating to, seed size, seed number, seed oil content, seed oil quality, seed flavour and toxicity, disease resistance, water use efficiency, nitrogen use efficiency, precocious flowering, flowering time, tree size, tree shape, growth rate, drought tolerance, salinity tolerance and/or growth in low-nutrient soils.
摘要翻译：提供了适用于Pongamia植物遗传标记的核酸序列扩增的引物和Pongamia植物的遗传分析方法。引物包含两个或三个核苷酸的重复单元重复五到十次，连同两个或三个核苷酸的三次延伸。还提供了通过引物扩增的遗传标记，从中可以制备用于Pongamia植物的遗传分析的另外的引物。引物，遗传标记和遗传分析方法可适用于具有所需性状或与种子大小，种子数，种子油含量，种子油质量，种子风味和毒性，疾病相关的所需性状的Pongamia植物的选择和育种耐旱性，水分利用效率，氮利用效率，早熟开花，开花时间，树木大小，树形状，生长速率，耐旱性，耐盐性和/或低营养土壤的生长。

5. 发明授权

US5413909A Method for profiling nucleic acids of unknown sequence using arbitrary oligonucleotide primers 失效
标题翻译：使用任意寡核苷酸引物分析未知序列核酸的方法
公开(公告)号：US5413909A
公开(公告)日：1995-05-09
申请号：US6380
申请日：1993-01-19
申请人： Brant J. Bassam , Gustavo Caetano-Anolles , Peter M. Gresshoff
发明人： Brant J. Bassam , Gustavo Caetano-Anolles , Peter M. Gresshoff
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6858 , C12N15/10 , C12Q1/686
摘要： A method for amplifying nucleic acid sequences in a template comprising at least one nucleic acid or mixture of nucleic acids wherein each nucleic acid comprises at least one strand. The method comprises the steps of treating the template with at least one oligonucleotide primer having a nucleic acid sequence that is substantially complementary to at least one nucleic acid sequence on the template under conditions such that the primer will bind to a segment that is substantially complementary to the primer, producing primed strands of nucleic acid. The primed strands produce extension strands, wherein the extension strands comprise the primer in combination with a sequence of nucleic acids that is substantially complementary to the template in a 5' to 3' direction from the primer. The extension strands are further treated with the primer producing amplification strands which are treated with primer. The amplification strands are repeatedly treated with primer to produce large numbers of amplification strands whereby the nucleic acid sequences of the amplification strands are substantially similar to nucleic acid sequences in the original template.
摘要翻译：一种用于扩增模板中的核酸序列的方法，其包含至少一种核酸或核酸混合物，其中每个核酸包含至少一个链。该方法包括以下步骤：用至少一种寡核苷酸引物处理模板，所述寡核苷酸引物具有与模板上至少一个核酸序列基本上互补的条件，使得引物将结合至基本上与引物，产生引发的核酸链。引发的链产生延伸链，其中延伸链包含与来自引物的5'至3'方向上的模板基本上互补的核酸序列的引物。延伸链进一步用引物产生的引物处理的引物进行处理。用引物重复处理扩增链以产生大量的扩增链，由此扩增链的核酸序列基本上与原始模板中的核酸序列相似。

6. 发明申请

US20150183839A1 SOYBEAN NODULATION REGULATORY PEPTIDES AND METHODS OF USE 审中-公开
标题翻译：大豆调节剂及其使用方法
公开(公告)号：US20150183839A1
公开(公告)日：2015-07-02
申请号：US13807676
申请日：2011-06-30
申请人： Peter M. Gresshoff , Brett Ferguson , Dugald Reid
发明人： Peter M. Gresshoff , Brett Ferguson , Dugald Reid
IPC分类号： C07K14/415 , C12N15/82 , C07K16/16
CPC分类号： C07K14/415 , C07K16/16 , C12N15/8261 , C12N15/8262 , Y02A40/146
摘要： Peptide fragments of CLAVATA3/ESR-related (CLE) proteins of soybean are provided which are activators of autoregulation of nodulation in soybean. CLE30 and CLE80 peptide fragments may act systemically while CLE60 peptides may act locally in the plant root. CLE30 and CLE80 are normally induced in response to Rhizobium inoculation of the plant root, while CLE60 acts as a nitrate or ammonium sensor. Also provided are interfering mutant CLE peptides and proteins, encoding nucleic acids and constructs for genetic modification of leguminous plants as a means for control of NARK-dependent nodulation and/or nitrogen fixation, particularly with regard to major leguminous crop plants such as soybean and common bean.
摘要翻译：提供了大豆CLAVATA3 / ESR相关蛋白（CLE）的肽片段，其是大豆中结瘤自动调节的活化剂。 CLE30和CLE80肽片段可以系统地起作用，而CLE60肽可以在植物根部局部作用。 CLE30和CLE80通常是针对根瘤菌接种植物根而引起的，而CLE60则作为硝酸盐或铵传感器。还提供了干扰性突变CLE肽和蛋白质，编码用于豆科植物遗传修饰的核酸和构建体作为用于控制NARK依赖性结瘤和/或固氮的手段，特别是关于主要豆科作物植物如大豆和常见豆。

7. 发明授权

US6074818A Fingerprinting of nucleic acids, products and methods 失效
标题翻译：指纹核酸，产品和方法
公开(公告)号：US6074818A
公开(公告)日：2000-06-13
申请号：US139459
申请日：1993-10-20
申请人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
发明人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
IPC分类号： G01N33/50 , C07H21/00 , C07H21/04 , C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6853 , Y10S435/81
摘要： Improvements to nucleic acid fingerprinting are disclosed. The method generates profiles of increased fidelity characteristic of the nucleic acids analyzed. Novel primers and other means are taught. Useful products are disclosed.
摘要翻译：公开了对核酸指纹图谱的改进。该方法产生所分析的核酸的增强的保真特征的分布。教导了新型底漆和其他方法。有用的产品被公开。

8. 发明授权

US5567585A Method and kit for silver staining, developing an image and visualizing biological materials 失效
标题翻译：用于银染的方法和试剂盒，开发图像和可视化生物材料
公开(公告)号：US5567585A
公开(公告)日：1996-10-22
申请号：US258553
申请日：1994-06-09
申请人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
发明人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
IPC分类号： C12Q1/68 , G01N27/447 , G01N27/26
CPC分类号： G01N27/44726 , C12Q1/6816
摘要： A silver staining method is provided for staining an organic molecule capable of binding silver. An improved image is developed and visualized. A kit useful in practicing the method is described. A permanent record of the image of the profile of the stained molecules is obtained.
摘要翻译：提供银染色方法来染色能够结合银的有机分子。改进的图像被开发和可视化。描述了在实践该方法中有用的试剂盒。获得染色分子轮廓图像的永久记录。

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