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1. 发明申请

US20070020727A1 Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof 审中-公开
标题翻译：特异性淀粉酶多肽，编码那些多肽的核酸及其用途
公开(公告)号：US20070020727A1
公开(公告)日：2007-01-25
申请号：US11523977
申请日：2006-09-19
申请人： Casper Berg , Kirsten Bojsen , Patrick Derkx , Carol Fioresi , Gijsbert Gerritse , Karsten Kragh , Wei Liu , Andrew Shaw , Charlotte Thoudahl , Anja Kellett-Smith
发明人： Casper Berg , Kirsten Bojsen , Patrick Derkx , Carol Fioresi , Gijsbert Gerritse , Karsten Kragh , Wei Liu , Andrew Shaw , Charlotte Thoudahl , Anja Kellett-Smith
IPC分类号： C12P21/06 , C07H21/04 , C12N9/32 , C12N15/74 , C12N1/21
CPC分类号： C12N9/2417
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.
摘要翻译：本发明涉及淀粉酶多肽和编码多肽的核酸及其用途。本发明的淀粉酶已被设计成具有更多的有益品质。具体地说，本发明的淀粉酶表现出改变的促进作用。

2. 发明申请

US20050136524A1 Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof 有权
标题翻译：特异性淀粉酶多肽，编码那些多肽的核酸及其用途
公开(公告)号：US20050136524A1
公开(公告)日：2005-06-23
申请号：US10886903
申请日：2004-07-07
申请人： Karsten Kragh , Kirsten Bojsen , Casper Berg , Patrick Derkx , Anja Kellett-Smith , Charlotte Thoudahl , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
发明人： Karsten Kragh , Kirsten Bojsen , Casper Berg , Patrick Derkx , Anja Kellett-Smith , Charlotte Thoudahl , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
IPC分类号： C07H21/04 , C12N9/28 , C12N9/32 , C12N15/74
CPC分类号： C12N9/2417
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.
摘要翻译：本发明涉及淀粉酶多肽和编码多肽的核酸及其用途。本发明的淀粉酶已被设计成具有更多的有益品质。具体地说，本发明的淀粉酶表现出改变的促进作用。

3. 发明申请

US20070020731A1 Thermostable amylase polypeptides, nucliec acids encoding those polypeptides and uses thereof 有权
标题翻译：热稳定淀粉酶多肽，编码这些多肽的核酸及其用途
公开(公告)号：US20070020731A1
公开(公告)日：2007-01-25
申请号：US11528999
申请日：2006-09-27
申请人： Karsten Kragh , Casper Berg , Patrick Derkx , Anja Kellett-Smith , Charlotte Thoudahl , Oene Veltman , Carol Fioresi , Gijsbert Gerritse , Wei Liu , Andrew Shaw
发明人： Karsten Kragh , Casper Berg , Patrick Derkx , Anja Kellett-Smith , Charlotte Thoudahl , Oene Veltman , Carol Fioresi , Gijsbert Gerritse , Wei Liu , Andrew Shaw
IPC分类号： C12P21/06 , C07H21/04 , C12N9/32 , C12N15/74 , C12N1/21
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： This is invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered thermostability.
摘要翻译：本发明涉及淀粉酶多肽和编码多肽的核酸及其用途。本发明的淀粉酶已被设计成具有更多的有益品质。具体地说，本发明的淀粉酶显示出改变的热稳定性。

4. 发明申请

US20050137111A1 Thermostable amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof 有权
标题翻译：热稳定淀粉酶多肽，编码那些多肽的核酸及其用途
公开(公告)号：US20050137111A1
公开(公告)日：2005-06-23
申请号：US10886905
申请日：2004-07-07
申请人： Karsten Kragh , Casper Berg , Patrick Derkx , Anja Kellett-Smith , Charlotte Thoudahl , Oene Veltman , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
发明人： Karsten Kragh , Casper Berg , Patrick Derkx , Anja Kellett-Smith , Charlotte Thoudahl , Oene Veltman , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
IPC分类号： A21D8/02 , A21D8/04 , A23L1/03 , A23L1/09 , A23L1/305 , C07H21/04 , C11D3/386 , C12N1/21 , C12N9/28 , C12N9/32 , C12N15/74
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered thermostability.
摘要翻译：本发明涉及淀粉酶多肽和编码多肽的核酸及其用途。本发明的淀粉酶已被设计成具有更多的有益品质。具体地说，本发明的淀粉酶显示出改变的热稳定性。

5. 发明申请

US20070141693A1 Polypeptide 审中-公开
标题翻译：多肽
公开(公告)号：US20070141693A1
公开(公告)日：2007-06-21
申请号：US11483220
申请日：2006-07-07
申请人： Casper Berg , Patrick Maria Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Kellet-Smith , Karsten Kragh , Wei Liu , Andrew Shaw , Bo Sorensen , Charlotte Thoudahl
发明人： Casper Berg , Patrick Maria Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Kellet-Smith , Karsten Kragh , Wei Liu , Andrew Shaw , Bo Sorensen , Charlotte Thoudahl
IPC分类号： C12N9/32 , C12N1/21
CPC分类号： A21D8/042 , A23L33/18 , C12N9/2411 , C12N9/2414 , C12N9/2417 , C12N9/2425 , C12N9/2428
摘要： The invention provides for a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity which may be selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.
摘要翻译：本发明提供可衍生自具有淀粉酶活性的亲本多肽的PS4变体多肽，其可以选自： 146，157，161，178，179，223，229，272，303，307，309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参考如SEQ ID NO：1所示的假单胞菌属酵母内切酶序列的位置编号，这种多肽作为食品或饲料添加剂的使用，以及编码其的核酸。

6. 发明授权

US06632643B2 Use of &agr;-1,4-glucan lyase for preparation of 1,5-D-hydrofructose 失效
标题翻译：使用α-1,4-葡聚糖裂解酶制备1,5-脱氢果糖
公开(公告)号：US06632643B2
公开(公告)日：2003-10-14
申请号：US09280197
申请日：1999-03-29
申请人： Shukun Yu , Kirsten Bojsen , Karsten Kragh , Maja Bojko , John Nielsen , Jan Marcussen , Tove Christensen
发明人： Shukun Yu , Kirsten Bojsen , Karsten Kragh , Maja Bojko , John Nielsen , Jan Marcussen , Tove Christensen
IPC分类号： C12P902
CPC分类号： C12P19/02 , C12N9/00 , C12N9/88
摘要： A method of preparing the sugar 1,5-D-anhydrofructose is described. The method comprises treating an &agr;-1,4-glucan with an &agr;-1,4-glucan lyase wherein the enzyme is used in substantially pure form. In a preferred embodiment, if the glucan contains links other than and in addition to the &agr;-1,4-links, the &agr;-1,4-glucan lyase is used in conjunction with a suitable reagent that can break the other links.
摘要翻译：描述了制备糖1,5-脱水果糖的方法。该方法包括用α-1,4-葡聚糖裂解酶处理α-1,4-葡聚糖，其中酶以基本上纯的形式使用。在优选的实施方案中，如果葡聚糖含有除了α-1,4-链节以外的链接，则将α-1,4-葡聚糖裂合酶与可以破坏其它链接的合适的试剂结合使用。 / PTEXT>

7. 发明授权

US6013504A .alpha.-1,4-glucan lyase from a fungus infected algae, its purification, gene cloning and expression in microorganisms 失效
标题翻译：来自真菌感染藻类的α-1,4-葡聚糖裂解酶，其纯化，基因克隆和在微生物中的表达
公开(公告)号：US6013504A
公开(公告)日：2000-01-11
申请号：US633768
申请日：1996-07-02
申请人： Shukun Yu , Kirsten Bojsen , Karsten Kragh , Maja Bojko , John Nielsen , Jan Marcussen
发明人： Shukun Yu , Kirsten Bojsen , Karsten Kragh , Maja Bojko , John Nielsen , Jan Marcussen
IPC分类号： C12N15/09 , C12N1/19 , C12N1/21 , C12N9/00 , C12N9/88 , C12N15/60 , C12P19/02 , C12R1/19 , C12R1/685 , C12R1/84 , C12R1/89 , C12R1/90
CPC分类号： C12P19/02 , C12N9/00 , C12N9/88
摘要： A method of preparing .alpha.-1,4-glucan lyase enzymes is described. The method comprises isolating the enzymes from a fungally infected algae. The amino acid sequences of the enzymes have been determined. The nucleic acid sequences coding for the enzymes have also been determined.
摘要翻译： PCT No.PCT / EP94 / 03399 Sec。 371日期：1996年7月2日 102（e）日期1996年7月2日PCT 1994年10月15日PCT PCT。公开号WO95 / 10618 日期1996年4月20日描述了制备α-1,4-葡聚糖裂解酶的方法。该方法包括从真菌感染的藻类中分离酶。已经确定了酶的氨基酸序列。也已经确定了编码酶的核酸序列。

8. 发明授权

US5908760A .alpha.-1,4-glucan lyase from a fungus, its purification gene cloning and expression in microorganisms 失效
标题翻译：来自真菌的α-1,4-葡聚糖裂解酶，其纯化基因在微生物中克隆和表达
公开(公告)号：US5908760A
公开(公告)日：1999-06-01
申请号：US633770
申请日：1996-07-08
申请人： Kirsten Bojsen , Shukun Yu , Karsten Kragh , Tove Christensen , Jan Marcussen
发明人： Kirsten Bojsen , Shukun Yu , Karsten Kragh , Tove Christensen , Jan Marcussen
IPC分类号： C12N15/09 , C12N9/88 , C12N15/60 , C12P19/02 , C12R1/645 , C12R1/685 , C12R1/84 , C12N15/00 , C07H21/04
CPC分类号： C12N9/88 , C12P19/02 , Y10S530/823
摘要： A method of preparing .varies.-glucan lyase enzymes is described. The method comprises isolating the enzymes from a culture of fungus wherein the culture is substantially free of any other organisms. Also described are the amino acid sequences for the enzymes and their coding sequences.
摘要翻译： PCT No.PCT / EP94 / 03398 Sec。 371日期：1996年7月8日 102（e）日期1996年7月8日PCT 1994年10月15日PCT PCT。公开号WO95 / 10617 日期：1995年4月20日描述了制备比例葡聚糖酶的方法。该方法包括从真菌培养物中分离酶，其中培养物基本上不含任何其它生物体。还描述了酶及其编码序列的氨基酸序列。

9. 发明申请

US20070072270A1 Polypeptide 有权
公开(公告)号：US20070072270A1
公开(公告)日：2007-03-29
申请号：US11635511
申请日：2006-12-08
申请人： Karsten Kragh , Harm Mulder , Steffen Petersen , Helle Fomsgaard , Oene Veltman
发明人： Karsten Kragh , Harm Mulder , Steffen Petersen , Helle Fomsgaard , Oene Veltman
IPC分类号： C12N9/24 , C12P21/06 , C07H21/04 , C12N1/21 , C12N15/74
CPC分类号： A21D8/042 , A23K20/189 , A23L29/35 , C12N9/2417 , C12Y302/0106
摘要： We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.

10. 发明申请

US20060018997A1 Polypeptide 审中-公开
标题翻译：多肽
公开(公告)号：US20060018997A1
公开(公告)日：2006-01-26
申请号：US10886527
申请日：2004-07-07
申请人： Karsten Kragh , Bo Sorensen
发明人： Karsten Kragh , Bo Sorensen
IPC分类号： A21D8/02 , C12N1/21
CPC分类号： C12N9/2417 , A21D8/042
摘要： We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 161 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. The PS4 variant polypeptide may further comprise one or more further mutations at a position selected from the group consisting of: 121 and 223, preferably 121F, 121Y, 121W, 223E and 223K, more preferably G121F, G121Y, G121W, G223E and/or G223K, or the group consisting of: 134, 141, 157, 223, 307, 334, 33 and 34, preferably G134R, A141P, I157L, G223A, H307L, S334P, N33Y and D34N. In preferred embodiments, the PS4 variant polypeptide further comprises a mutation at position 87, preferably G87S, a mutation at position 178, preferably L178F, and/or a mutation at position 179, preferably A179T.
摘要翻译：我们描述了一种包含PS4变体多肽的食品添加剂，其中PS4变体多肽可衍生自具有非麦芽糖外切酶活性的亲本多肽，其中PS4变体多肽包含参考位置编号在161位置的氨基酸取代的SEQ ID NO：1所示的假单胞菌糖酵母外切酶序列.PS4变体多肽可以在选自121和223，优选121F，121Y，121W，223E和223K的位置进一步包含一个或多个另外的突变，更优选G121F，G121Y，G121W，G223E和/或G223K，或由134,141,157,223,307,334,33和34，优选G134R，A141P，I157L，G223A，H307L，S334P， N33Y和D34N。在优选的实施方案中，PS4变体多肽还包含位置87，优选G87S，178位突变，优选L178F和/或179位突变，优选A179T的突变。

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