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1. 发明申请

WO2005066619A1 MIXED MODE MICROFLUIDIC METHOD 审中-公开
标题翻译：混合模式微流化方法
公开(公告)号：WO2005066619A1
公开(公告)日：2005-07-21
申请号：PCT/US2004/040635
申请日：2004-12-06
申请人： CALIPER LIFE SCIENCES, INC. , SPAID, Michael, A.
发明人： SPAID, Michael, A.
IPC分类号： G01N27/447
CPC分类号： G01N27/44743 , B01F13/0076 , B01L3/502784 , B01L2200/0631 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2400/0415 , B01L2400/0421 , B01L2400/0487 , F04B19/006 , G01N27/44704 , G01N27/44773 , G01N27/44791 , H02N11/006
摘要： A method of extracting a charged species from a sample and concentrating it, in an interconnected channel network with a first (1208), second (1210) and third (1212) channel segment which intersect in a first fluid junction, the second channel terminating in a first fluid reservoir (1204), and the third channel segment (1212) intersecting at a second fluid junction (1215) a fourth channel segment (1214) which terminates in a second fluid reservoir (1206) containing a high ionic strenght buffer. The sample contains charged species in a low ionic strenght buffer and is flowed through the channel network by applying a first pressure differential across the first (1208) and second (1210) segments, and a smaller second pressure differential across the third (1212) and fourth (1214) segments. A voltage is applied across the first (1204) and second (1206) reservoirs, which is sufficient to cause a substantial part of the charged species in the sample in the first fluid junction to flow into the third channel segment (1212).
摘要翻译：一种从样品中提取带电物质并将其集中在与在第一流体结中相交的第一（1208），第二（1210）和第三（1212）通道段的互连通道网络中的第二通道终止于第一流体储存器（1204）和与第二流体结（1215）相交的第三通道段（1212），第四通道段（1214）终止于含有高离子强度缓冲液的第二流体储存器（1206）中。样品在低离子强度缓冲液中含有带电荷的物质，并且通过在第一（1208）和第二（1210）段上施加第一压差而流过通道网络，并且跨越第三（1212）和第四（1214）个细分。跨越第一（1204）和第二（1206）储存器施加电压，其足以使得第一流体结中的样品中的大部分带电物质流入第三通道段（1212）。

2. 发明申请

WO2005113148A1 SINGLE MOLECULE AMPLIFICATION AND DETECTION OF DNA LENGTH 审中-公开
标题翻译：单分子放大和DNA长度的检测
公开(公告)号：WO2005113148A1
公开(公告)日：2005-12-01
申请号：PCT/US2005/017065
申请日：2005-05-16
申请人： CALIPER LIFE SCIENCES, INC. , KNAPP, Michael, R. , BAKER, Jill, M. , CHOW, Andrea, W. , KOPF-SILL, Anne, R. , SPAID, Michael, A.
发明人： KNAPP, Michael, R. , BAKER, Jill, M. , CHOW, Andrea, W. , KOPF-SILL, Anne, R. , SPAID, Michael, A.
IPC分类号： B01L3/00
CPC分类号： C12Q1/6851 , B01L3/5027 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/10 , B01L2200/16 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2300/1833 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12Q1/6818 , C12Q1/6837 , C12Q1/686 , C12Q1/6869 , G01N21/6428 , G01N2021/6441 , C12Q2565/102 , C12Q2537/143 , C12Q2545/113 , C12Q2531/113 , C12Q2565/629 , C12Q2527/143
摘要： Methods and systems for performing single molecule amplification for detection, quantification and statistical analysis of nucleic acids are provided. Methods and systems are provided for determining and quantifying lengths of nucleic acids of interest.
摘要翻译：提供了用于进行单分子扩增用于核酸检测，定量和统计分析的方法和系统。提供了用于确定和定量感兴趣的核酸的长度的方法和系统。

3. 发明申请

WO2005075967A1 METHODS, DEVICES AND SYSTEMS FOR CHARACTERIZING PROTEINS 审中-公开
标题翻译：用于表征蛋白质的方法，装置和系统
公开(公告)号：WO2005075967A1
公开(公告)日：2005-08-18
申请号：PCT/US2005/002746
申请日：2005-01-31
申请人： CALIPER LIFE SCIENCES, INC. , CHOW, Andrea , FATHOLLAHI, Bahram , MIKKELSEN, James, C., Jr. , SPAID, Michael, A. , WINOTO, Adrian
发明人： CHOW, Andrea , FATHOLLAHI, Bahram , MIKKELSEN, James, C., Jr. , SPAID, Michael, A. , WINOTO, Adrian
IPC分类号： G01N27/26
CPC分类号： G01N27/44747 , C07K1/24 , G01N27/44791
摘要： A method of characterizing a polypeptide, comprising providing a first capillary channel having a separation buffer disposed within, wherein the separation buffer comprises a non-crosslinked polymer solutin, a buffering aten, a detergent, and a lipophilic dye. The separation buffer is provided such that, at the time of detection, the detergent concdntration in the buffer is not above the critical micelle concentration. The polypeptide is introduced into one end of the capillary channel. An electric field is applied across a length of the capillary channel, which transports polypeptides of different sizes through the polymer solution at different rates. The polypeptides is then detected as it passes a point along the length of the capillary channel.
摘要翻译：一种表征多肽的方法，包括提供具有设置在其内的分离缓冲液的第一毛细通道，其中所述分离缓冲液包含非交联聚合物溶液，缓冲液，洗涤剂和亲脂性染料。提供分离缓冲液，使得在检测时，缓冲液中的洗涤剂浓度不高于临界胶束浓度。将多肽引入毛细管通道的一端。在毛细通道的一段长度上施加电场，毛细通道以不同的速率输送不同尺寸的多肽通过聚合物溶液。然后当多肽经过沿着毛细通道长度的点时被检测。

4. 发明公开

EP1755785A1 SINGLE MOLECULE AMPLIFICATION AND DETECTION OF DNA LENGTH 审中-公开
标题翻译：单个分子的扩增和DNA长度的检测
公开(公告)号：EP1755785A1
公开(公告)日：2007-02-28
申请号：EP05749873.5
申请日：2005-05-16
申请人： Caliper Life Sciences, Inc.
发明人： KNAPP, Michael, R. , BAKER, Jill, M. , CHOW, Andrea, W. , KOPF-SILL, Anne, R. , SPAID, Michael, A.
IPC分类号： B01L3/00 , C12Q1/68
CPC分类号： C12Q1/6851 , B01L3/5027 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/10 , B01L2200/16 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2300/1833 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12Q1/6818 , C12Q1/6837 , C12Q1/686 , C12Q1/6869 , G01N21/6428 , G01N2021/6441 , C12Q2565/102 , C12Q2537/143 , C12Q2545/113 , C12Q2531/113 , C12Q2565/629 , C12Q2527/143
摘要： Methods and systems for performing single molecule amplification for detection, quantification and statistical analysis of nucleic acids are provided. Methods and systems are provided for determining and quantifying lengths of nucleic acids of interest.

5. 发明公开

EP1709435A1 METHODS, DEVICES AND SYSTEMS FOR CHARACTERIZING PROTEINS 审中-公开
标题翻译：方法，装置和系统表征蛋白的
公开(公告)号：EP1709435A1
公开(公告)日：2006-10-11
申请号：EP05712259.0
申请日：2005-01-31
申请人： Caliper Life Sciences, Inc.
发明人： CHOW, Andrea , FATHOLLAHI, Bahram , MIKKELSEN, James, C., Jr. , SPAID, Michael, A. , WINOTO, Adrian
IPC分类号： G01N27/26 , C07K1/24
CPC分类号： G01N27/44747 , C07K1/24 , G01N27/44791
摘要： A method of characterizing a polypeptide, comprising providing a first capillary channel having a separation buffer disposed within, wherein the separation buffer comprises a non-crosslinked polymer solutin, a buffering aten, a detergent, and a lipophilic dye. The separation buffer is provided such that, at the time of detection, the detergent concdntration in the buffer is not above the critical micelle concentration. The polypeptide is introduced into one end of the capillary channel. An electric field is applied across a length of the capillary channel, which transports polypeptides of different sizes through the polymer solution at different rates. The polypeptides is then detected as it passes a point along the length of the capillary channel.

6. 发明公开

EP1697733A1 MIXED MODE MICROFLUIDIC METHOD 审中-公开
标题翻译：微流控程序在混合模式
公开(公告)号：EP1697733A1
公开(公告)日：2006-09-06
申请号：EP04813030.6
申请日：2004-12-06
申请人： Caliper Life Sciences, Inc.
发明人： SPAID, Michael, A.
IPC分类号： G01N27/447 , B01L3/00
CPC分类号： G01N27/44743 , B01F13/0076 , B01L3/502784 , B01L2200/0631 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2400/0415 , B01L2400/0421 , B01L2400/0487 , F04B19/006 , G01N27/44704 , G01N27/44773 , G01N27/44791 , H02N11/006
摘要： A method of extracting a charged species from a sample and concentrating it, in an interconnected channel network with a first (1208), second (1210) and third (1212) channel segment which intersect in a first fluid junction, the second channel terminating in a first fluid reservoir (1204), and the third channel segment (1212) intersecting at a second fluid junction (1215) a fourth channel segment (1214) which terminates in a second fluid reservoir (1206) containing a high ionic strenght buffer. The sample contains charged species in a low ionic strenght buffer and is flowed through the channel network by applying a first pressure differential across the first (1208) and second (1210) segments, and a smaller second pressure differential across the third (1212) and fourth (1214) segments. A voltage is applied across the first (1204) and second (1206) reservoirs, which is sufficient to cause a substantial part of the charged species in the sample in the first fluid junction to flow into the third channel segment (1212).

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