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1. 发明申请

WO2003025185A1 A METHOD TO MODIFY CELL NUMBER, ARCHITECTURE AND YIELD OF PLANTS BY OVEREXPRESSING THE E2F TRANSCRIPTION FACTOR 审中-公开
标题翻译：通过过度表达E2F转录因子修饰植物细胞数，构建和产量的方法
公开(公告)号：WO2003025185A1
公开(公告)日：2003-03-27
申请号：PCT/EP2002/010236
申请日：2002-09-12
申请人： CROPDESIGN N.V. , BEECKMAN, Tom , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir , BROEKAERT, Willem , DILLEN, Willy , FRANKARD, Valerie
发明人： BEECKMAN, Tom , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir , BROEKAERT, Willem , DILLEN, Willy , FRANKARD, Valerie
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to a method for modifying plant growth and development processes by affecting cell cycle regulation comprising modulating expression of members of the plant E2F transcription factor family. More specific the invention relates to methods for stimulating cell division in a plant. The invention further relates to transgenic plants produced by the methods of the invention having altered plant growth and development characteristics compared to their isogenic counterparts. Preferably, the plant characteristics modified by the present invention include enlarged cotyledons, enhanced growth rate, stress resistance and seedling survival, more cells, more tillers, more panicles.
摘要翻译：本发明涉及通过影响细胞周期调节来修饰植物生长和发育过程的方法，其包括调节植物E2F转录因子家族成员的表达。更具体地，本发明涉及刺激植物细胞分裂的方法。本发明还涉及通过与其同基因对应物相比具有改变的植物生长和发育特征的本发明方法生产的转基因植物。优选地，通过本发明修饰的植物特征包括扩大的子叶，增强的生长速率，抗胁迫性和幼苗存活，更多的细胞，更多的分蘖，更多的穗。

2. 发明申请

WO2007054522A1 PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND A METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有改善生长特性的植物及其制备方法
公开(公告)号：WO2007054522A1
公开(公告)日：2007-05-18
申请号：PCT/EP2006/068248
申请日：2006-11-08
申请人： CROPDESIGN N.V. , VIB VZW , UNIVERSITEIT GENT , DE MEUTTER, Jan , MIRONOV, Vladimir , REUZEAU, Christophe , INZE, Dirk , VLIEGHE, Kobe , DE VEYLDER, Lieven
发明人： DE MEUTTER, Jan , MIRONOV, Vladimir , REUZEAU, Christophe , INZE, Dirk , VLIEGHE, Kobe , DE VEYLDER, Lieven
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8261 , C12N15/8271 , Y02A40/146
摘要： The present invention concerns a method for increasing plant yield and/or increased stress resistance by decreasing the level of activity of a DEL1 polypeptide. One such method comprises introducing into a plant a nucleic acid comprising a variant DEL1 nucleic acid. Another method comprises downregulating expression of a DEL1 gene . The invention also relates to transgenic plants having introduced therein a variant DEL1 nucleic acid thereof, which plants have increased yield and/or increased stress resistance relative to control plants. The present invention also concerns a novel DEL1 protein and its encoding sequence, and constructs useful in the methods of the invention.
摘要翻译：本发明涉及通过降低DEL1多肽的活性水平来增加植物产量和/或增加的胁迫抗性的方法。一种这样的方法包括向植物中引入包含变体DEL1核酸的核酸。另一种方法包括下调DEL1基因的表达。本发明还涉及其中引入了其变体DEL1核酸的转基因植物，该植物相对于对照植物具有增加的产量和/或增加的胁迫抗性。本发明还涉及一种新颖的DEL1蛋白及其编码序列，以及可用于本发明方法的构建体。

3. 发明申请

WO2003018818A2 METHOD AND MEANS FOR MODULATING PLANT CELL CYCLE PROTEINS AND THEIR USE IN PLANT CELL GROWTH CONTROL 审中-公开
标题翻译：调节植物细胞周期蛋白的方法和手段及其在植物细胞生长控制中的应用
公开(公告)号：WO2003018818A2
公开(公告)日：2003-03-06
申请号：PCT/EP2002/009504
申请日：2002-08-17
申请人： CROPDESIGN N.V. , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir , SEGERS, Gerda
发明人： DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir , SEGERS, Gerda
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention relates to modulation of endoreduplication in a plant or part thereof which may be achieved by modifying expression or activity of E2F or E2F in combination with DP.Modulation of endoreduplication has an advantageous influence on plant cell size and storage capacity of plant cells.
摘要翻译：本发明涉及通过改变E2F或E2F与DP组合的表达或活性来实现的植物或其部分中的内切复制的调节。内切复制的调节对植物细胞的大小和储存能力具有有利影响。

4. 发明申请

WO2007051866A3 PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND A METHOD FOR MAKING THE SAME 审中-公开
公开(公告)号：WO2007051866A3
公开(公告)日：2007-05-10
申请号：PCT/EP2006/068190
申请日：2006-11-07
申请人： CROPDESIGN N.V. , SANZ MOLINERO, Ana Isabel , REUZEAU, Christophe , HATZFELD, Yves , BOUDOLF, Veronique , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir
发明人： SANZ MOLINERO, Ana Isabel , REUZEAU, Christophe , HATZFELD, Yves , BOUDOLF, Veronique , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
摘要： The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (referred to as YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (hereinafter referred to as YEP16); or having modulated expression of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.

5. 发明申请

WO2007051866A2 PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND A METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有改进的增长特性的植物及其制备方法
公开(公告)号：WO2007051866A2
公开(公告)日：2007-05-10
申请号：PCT/EP2006/068190
申请日：2006-11-07
申请人： CROPDESIGN N.V. , SANZ MOLINERO, Ana Isabel , REUZEAU, Christophe , HATZFELD, Yves , BOUDOLF, Veronique , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir
发明人： SANZ MOLINERO, Ana Isabel , REUZEAU, Christophe , HATZFELD, Yves , BOUDOLF, Veronique , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N9/1205 , C07K14/415 , C12N15/8261 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (referred to as YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (hereinafter referred to as YEP16); or having modulated expression of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.
摘要翻译：本发明一般涉及分子生物学领域，涉及相对于相应的野生型植物改善植物生长特性的方法。更具体地说，本发明涉及一种改善植物生长特性的方法，包括调节编码I类同源结构域亮氨酸拉链（HDZip）hox5多肽或其同源物的核酸在植物中的表达; 或包括调节编码硝酸盐转运蛋白（NRT）或其同源物的核酸在植物中的表达; 或包括调节编码称为产量增强蛋白16（称为YEP16）的多肽的核酸在植物中的表达; 或包括调节植物中I族糖原合酶激酶（I族类似毛癣菌激酶）或其同系物的表达。本发明还涉及具有调节的表达编码I类同源结构域亮氨酸拉链（HDZip）hox5多肽或其同源物的核酸的植物; 或调节了编码硝酸盐转运蛋白（NRT）或其同源物的核酸的表达; 或调节了表达产量增强蛋白16（以下称为YEP16）的编码多肽的核酸的表达; 或具有调节的第I组糖原合酶激酶（第I组类似物激酶）或其同源物的表达，所述植物相对于相应的野生型植物具有改善的生长特性。本发明还提供了可用于本发明方法的构建体。

6. 发明申请

WO2002083901A2 PLANT GROWTH REGULATING GENES, PROTEINS AND USES THEREOF 审中-公开
标题翻译：植物生长调节基因，蛋白质及其用途
公开(公告)号：WO2002083901A2
公开(公告)日：2002-10-24
申请号：PCT/EP2002/004035
申请日：2002-04-11
申请人： CROPDESIGN N.V. , SAUTER, Margret , LORBIECKE, René , MIRONOV, Vladimir , FRANKARD, Valérie , DILLEN, Willy , LEJEUNE, Pierre , BROEKAERT, Willem
发明人： SAUTER, Margret , LORBIECKE, René , MIRONOV, Vladimir , FRANKARD, Valérie , DILLEN, Willy , LEJEUNE, Pierre , BROEKAERT, Willem
IPC分类号： C12N15/29
CPC分类号： C12N15/8262 , C07K14/415 , C12N15/8237 , C12N15/8261 , Y02A40/146
摘要： Provided are methods for controlling or altering the growth characteristics of a plant or of an organ or tissue or cell of this plant. This method comprises introduction and/or expression of one or more Growth Regulating Proteins functional in a plant or parts thereof and/or one or more DNA sequences encoding such proteins. Another method comprises contacting a plant or plant tissue with the purified Growth Regulated Protein or the active product derived from it. Provided are also DNA sequences encoding plant Growth Regulating Protein(s) as well as methods for obtaining the same. Furthermore, vectors comprising said DNA sequences are described, wherein the DNA sequences are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, proteins encoded by said DNA sequences, antibodies to said proteins and methods for their production are provided. Furthermore, regulatory sequences, which naturally regulate the expression of the above-described DNA sequences, are described. Methods for the identification of compounds being capable of interacting with said Growth Regulating Proteins are described as well. Furthermore, transgenic plant cells, plant tissues and plants containing the above-described DNA sequences and vectors are described, as well as the use of the aforementioned DNA sequences, vectors, proteins, antibodies, regulatory sequences and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture.
摘要翻译：提供了用于控制或改变植物或该植物的器官或组织或细胞的生长特性的方法。该方法包括在植物或其部分和/或编码这种蛋白质的一个或多个DNA序列中引入和/或表达一种或多种功能的生长调节蛋白。另一种方法包括使植物或植物组织与纯化的生长调节蛋白或其衍生的活性产物接触。还提供编码植物生长调节蛋白的DNA序列以及获得其的方法。此外，描述了包含所述DNA序列的载体，其中所述DNA序列可操作地连接到允许在原核和/或真核宿主细胞中表达的调节元件。此外，提供了由所述DNA序列编码的蛋白质，对所述蛋白质的抗体及其生产方法。此外，描述了调节序列，其自然调节上述DNA序列的表达。还描述了用于鉴定能够与所述生长调节蛋白相互作用的化合物的方法。此外，描述了含有上述DNA序列和载体的转基因植物细胞，植物组织和植物，以及使用上述DNA序列，载体，蛋白质，抗体，调节序列和/或化合物本发明在植物细胞和组织培养，植物育种和/或农业中。

7. 发明申请

WO2005024029A2 PLANTS HAVING MODIFIED GROWTH CHARACTERISTICS AND METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有改良增长特性的植物及其制造方法
公开(公告)号：WO2005024029A2
公开(公告)日：2005-03-17
申请号：PCT/EP2004/052035
申请日：2004-09-03
申请人： CROPDESIGN N.V. , BROEKAERT, Willem , FRANKARD, Valerie , HATZFELD, Yves , MIRONOV, Vladimir
发明人： BROEKAERT, Willem , FRANKARD, Valerie , HATZFELD, Yves , MIRONOV, Vladimir
IPC分类号： C12N15/82
CPC分类号： C12N9/1205 , C12N15/8262 , C12Q1/485 , G01N2333/39 , G01N2500/10
摘要： The present invention concerns a method for modifying the growth characteristics of plants by modulating expression in a plant of a B-type CDK nucleic acid and/or modulating activity and/or levels in a plant of a B-type CDK protein. The invention also relates to transgenic plants having modified growth characteristics, which plants have modulated expression of a B-type CDK nucleic acid and/or modulated activity and/or levels in a plant of a B-type CDK protein relative to expression, activity and/or levels in corresponding wild-type plants. The present invention also provides a novel screening method for the identification of mutant CDKs having enhanced CDK activity relative to corresponding non-mutated CDKs. The present invention also provides a novel screening method for the identification of non-active CDKs that are able to bind to CKIs (cyclin dependent kinase inhibitors). The invention also provides mutant CDKs obtainable by the screening methods according to the invention.
摘要翻译：本发明涉及通过调节植物中B型CDK核酸的表达和/或调节植物中B的活性和/或水平来修饰植物生长特性的方法型CDK蛋白。本发明还涉及具有修饰的生长特征的转基因植物，所述植物调节B型CDK核酸的表达和/或调节B型CDK蛋白的植物中的活性和/或水平相对于表达，活性和 /或相应野生型植物中的水平。本发明还提供了用于鉴定相对于相应的未突变的CDK具有增强的CDK活性的突变型CDK的新型筛选方法。本发明还提供了用于鉴定能够结合CKI（细胞周期蛋白依赖性激酶抑制剂）的非活性CDK的新型筛选方法。本发明还提供了通过根据本发明的筛选方法可获得的突变型CDK。

8. 发明申请

WO2003027299A2 A PLANT CYCLIN DEPENDENT KINASE-LIKE PROTEIN, ITS INTERACTORS AND USES THEREOF 审中-公开
标题翻译：植物细胞依赖性激酶样蛋白，其相互作用及其用途
公开(公告)号：WO2003027299A2
公开(公告)日：2003-04-03
申请号：PCT/EP2002/010364
申请日：2002-09-16
申请人： CROPDESIGN NV , DE VEYLDER, Lieven , DE PINHO BAROCCO, Rosa, Maria , MIRONOV, Vladimir , INZE, Dirk
发明人： DE VEYLDER, Lieven , DE PINHO BAROCCO, Rosa, Maria , MIRONOV, Vladimir , INZE, Dirk
IPC分类号： C12N15/82
CPC分类号： C12N9/1205 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to methods for modifying plant growth and development processes comprising modulating expression of a plant cyclin dependent kinase-like gene and/or one of its interacting proteins or homologues, derivatives or fragments thereof. The invention further relates to the use of vectors for performing the present invention and to transgenic plants produced therewith having altered plant growth and development characteristics compared to their isogenic counterparts. Preferably, the characteristics modified by the present invention include growth rate, yield, senescence, flowering and photosynthesis.
摘要翻译：本发明涉及用于修饰植物生长和发育过程的方法，包括调节植物细胞周期蛋白依赖性激酶样基因和/或其相互作用蛋白质或其同系物，衍生物或片段之一的表达。本发明还涉及用于实施本发明的载体和与其生产的转基因植物相比，其与其同基因对应物相比具有改变的植物生长和发育特征。优选地，本发明修饰的特征包括生长速率，产量，衰老，开花和光合作用。

9. 发明申请

WO2005061702A2 PLANTS HAVING INCREASED YIELD AND METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有增加产量的植物及其制备方法
公开(公告)号：WO2005061702A2
公开(公告)日：2005-07-07
申请号：PCT/EP2004/053683
申请日：2004-12-22
申请人： CROPDESIGN N.V. , FRANKARD, Valerie , MIRONOV, Vladimir
发明人： FRANKARD, Valerie , MIRONOV, Vladimir
IPC分类号： C12N15/00
CPC分类号： C12N15/8261 , Y02A40/146
摘要： The invention concerns a method for increasing plant yield by introducing into a plant a cyclin A nucleic acid, preferably encoding a cyclin A protein, which cyclin A nucleic acid is operably linked to a seed-preferred promoter. By using this method, plant yield may be increased in optimal and sub-optimal growing conditions. The method results in plants having increased yield relative to corresponding wild type plants and relative to transgenic plants constitutively expressing cyclin A.
摘要翻译：本发明涉及通过将植物细胞周期蛋白A核酸（优选编码细胞周期蛋白A蛋白）引入植物来提高植物产量的方法，该细胞周期蛋白A核酸可操作地连接到种子优选的启动子。通过使用该方法，可以在优化和次优生长条件下提高植物产量。该方法导致植物相对于相应的野生型植物和相对于组成型表达细胞周期蛋白A的转基因植物具有增加的产量。

10. 发明申请

WO2005059147A2 PLANTS HAVING MODIFIED GROWTH CHARACTERISTICS AND METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有改良生长特性的植物及其制备方法
公开(公告)号：WO2005059147A2
公开(公告)日：2005-06-30
申请号：PCT/EP2004/053594
申请日：2004-12-17
申请人： CROPDESIGN N.V. , FRANKARD, Valerie , MIRONOV, Vladimir , SANZ MOLINERO, Ana Isabel
发明人： FRANKARD, Valerie , MIRONOV, Vladimir , SANZ MOLINERO, Ana Isabel
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： The present invention concerns a method for modifying the growth characteristics of plants by modulating expression in a plant of a nucleic acid sequence encoding a GRUBX protein and/or modulating activity and/or levels in a plant of a GRUBX protein. The present invention furthermore provides novel GRUBX proteins and nucleic acids encoding such proteins. The invention also relates to constructs comprising GRUBX encoding nucleic acids, and transgenic plants having modified growth characteristics, which plants have modulated expression of a nucleic acid encoding a GRUBX protein.
摘要翻译：本发明涉及一种通过调节植物中编码GRUBX蛋白的核酸序列表达和/或调节GRUBX蛋白植物中的活性和/或水平来修饰植物生长特性的方法。本发明还提供了编码这种蛋白质的新型GRUBX蛋白质和核酸。本发明还涉及包含GRUBX编码核酸的构建体和具有修饰生长特性的转基因植物，该植物已经调节了编码GRUBX蛋白质的核酸的表达。

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