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    • 4. 发明申请
    • HIGH AMYLOSE WHEAT - III
    • 高级小麦 - III
    • WO2018006126A1
    • 2018-01-11
    • PCT/AU2017/050693
    • 2017-07-04
    • COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION
    • LI, XinguoLI, ZhongyiREGINA, AhmedJOBLING, Stephen Alan
    • A01H5/00A01H1/04A01H1/06
    • The present invention provides wheat grain of the species Triticum aestivum , the grain comprising i) mutations in each of its SSIIa genes such that the grain is homozygous for a mutation in its SSIIa-A gene, homozygous for a mutation in its SSIIa-B gene and homozygous for a mutation in its SSIIa-D gene, wherein at least two of the mutations in said SSIIa genes are null mutations, ii) a total starch content comprising an amylose content and an amylopectin content, iii) a fructan content which is increased relative to wild-type wheat grain on a weight basis, preferably between 3% and 12% of the grain weight, iv) a β-glucan content, v) an arabinoxylan content, vi) a cellulose content. The grain has a weight of between 25mg and 60mg, and the amylose content is between 45% and 70% on a weight basis of the total starch content of the grain as determined by iodine binding assay. The amylopectin content on a weight basis is reduced relative to the wild-type wheat grain, and each of the β-glucan content, arabinoxylan content and cellulose content are increased relative to the wild-type wheat grain on a weight basis, such that the sum of the fructan content, β-glucan content, arabinoxylan content and cellulose content is between 15% and 30% of the grain weight.
    • 本发明提供了普通小麦品种的小麦籽粒,所述籽粒包含i)其每种SSIIa基因中的突变,使得谷粒 对于其SSIIa-A基因中的突变是纯合的,其SSIIa-B基因中的突变是纯合的并且对于其SSIIa-D基因中的突变是纯合的, 其中所述SSIIa基因中的至少两个突变为无效突变,ii)包含直链淀粉含量和支链淀粉含量的总淀粉含量,iii)增加的果聚糖含量 相对于野生型小麦谷粒重量,优选谷粒重量的3%至12%,iv)β-葡聚糖含量,v)阿拉伯木聚糖含量,vi)纤维素含量。 通过碘结合测定法测定,谷物的重量为25mg至60mg,且直链淀粉含量以谷物总淀粉含量的重量计为45%至70%。 基于重量的支链淀粉含量相对于野生型小麦谷粒减少,并且每种β-葡聚糖含量,阿拉伯木聚糖含量和纤维素含量相对于野生型小麦谷粒以重量为基础增加,使得 果聚糖含量,β-葡聚糖含量,阿拉伯木聚糖含量和纤维素含量的总和在谷物重量的15%至30%之间。