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1. 发明申请

WO0183691A2 SYSTEM FOR IDENTIFYING AND ANALYZING EXPRESSION OF ARE-CONTAINING GENES 审中-公开
标题翻译：用于识别和分析含有基因的表达的系统
公开(公告)号：WO0183691A2
公开(公告)日：2001-11-08
申请号：PCT/US0111993
申请日：2001-04-12
申请人： CLEVELAND CLINIC FOUNDATION , KING FAISAL SPECIALIST HOSPITA , ABU KHABAR KHALID S , WILLIAMS BRYAN R G , FREVEL MATHIAS , SILVERMAN ROBERT H
发明人： ABU-KHABAR KHALID S , WILLIAMS BRYAN R G , FREVEL MATHIAS , SILVERMAN ROBERT H
IPC分类号： C12N15/09 , C12M1/00 , C12M1/34 , C12N20060101 , C12Q1/68 , G01N33/483 , G06F19/20 , G06F19/22 , G06F19/28 , C12N
CPC分类号： G06F19/28 , C12Q1/6897 , G06F19/20 , G06F19/22
摘要： Adenylate-uridylate-rich (ARE) elements present in the 3' untranslated region (UTR) of gene and mRNA sequences are disclosed. THE ARE motif comprises a sequence encompassed by: SEQ ID NO: 1 or 2, with further limitations. Computational methods of identifying genes or coding sequences in a database which comprise ARE elements are disclosed. The computational methods can be used for gene discovery, and sequence analysis. Methods of identifying, isolating and amplyfying ARE-element-associated polynucleotides using PCR, RT-PCR, hybridization, etc. are disclosed. PCR primers, oligonucleotide arrays, polynucleotide libraries, computer programs, and computer systems relating to ARE elements are disclosed.
摘要翻译：公开了存在于基因和mRNA序列的3'非翻译区（UTR）中的腺苷酸 - 富含尿苷酸（ARE）的元件。 ARE基序包含由SEQ ID NO：1或2包含的序列，其进一步的限制。公开了在包含ARE元素的数据库中识别基因或编码序列的计算方法。计算方法可用于基因发现和序列分析。公开了使用PCR，RT-PCR，杂交等鉴定，分离和纯化ARE-元件相关多核苷酸的方法。公开了与ARE元件相关的PCR引物，寡核苷酸阵列，多核苷酸文库，计算机程序和计算机系统。

2. 发明专利

AU5534401A System for identifying and analyzing expression of are-containing genes 未知
公开(公告)号：AU5534401A
公开(公告)日：2001-11-12
申请号：AU5534401
申请日：2001-04-12
申请人： CLEVELAND CLINIC FOUNDATION , KING FAISAL SPECIALIST HOSPITA
发明人： ABU-KHABAR KHALID S , WILLIAMS BRYAN R G , FREVEL MATHIAS , SILVERMAN ROBERT H
IPC分类号： C12N15/09 , C12M1/00 , C12M1/34 , C12N20060101 , C12Q1/68 , C12Q1/6897 , G01N33/483 , G06F19/20 , G06F19/22 , G06F19/28
摘要： The present invention relates to a gene discovery system and gene expression systems specific for genes encoding ARE-containing mRNAs. In one aspect, the present invention relates to computational methods of selecting coding sequences of ARE-genes from databases using a one or more ARE search sequences. The ARE search sequences are from 10 to 80 nucleotides in length and comprise a sequence which is encompassed by one of the following two sequences: (a) WU/T(AU/TU/TU/TA)TWWW, SEQ ID NO. 1, wherein none or one of the nucleotides outside of the parenthesis is replaced by a different nucleotide, and wherein W represents A, U, or T; and (b) U/T(AU/TU/T/U/T)n, SEQ ID NO. 2 wherein n indicates that the search sequence comprises from 3 to 12 of the tetrameric sequences contained within the parenthesis. The method comprises extracting from the databases, those nucleic acids whose protein coding sequences are upstream and contiguous with a 3' untranslated region (UTR) that comprises one of the ARE search sequences. The present invention also relates to methods of selectively amplifying RNA and cDNA molecules using primers derived from and complementary to the consensus 5' sequence motifs and primers derived from and complementary to the ARE search sequence. The present invention also relates to methods of selectively amplifying ARE genes which employ a 3' primer which is from 15 to 50 nucleotides and length and comprises from 2 to 10 pentamers having the sequence TAAAT. The pentameric sequences in the primers are either overlapping or non-overlapping. The 3' primers are used in the reverse transcription step of the methods, the polymerase chain reaction (PCR) amplification step of the methods, or in both the reverse transcription step and the PCR amplification step of the methods. The present invention also relates to methods of making libraries which comprise portions of the ARE genes that are selectively amplified by the present methods and to methods of making microarrays which comprise probes that hybridize under stringent conditions to portions of the protein coding sequences of the ARE genes that are selectively amplified by the present methods. The present invention also relates to libraries and the microarrays that are made by such methods.

3. 发明公开

EP1410301A4 SYSTEM FOR IDENTIFYING AND ANALYZING EXPRESSION OF ARE-CONTAINING GENES 审中-公开
标题翻译： SYSTEM FOR识别和分析基因的表达是包含
公开(公告)号：EP1410301A4
公开(公告)日：2008-01-23
申请号：EP01928494
申请日：2001-04-12
申请人： CLEVELAND CLINIC FOUNDATION , KING FAISAL SPECIALIST HOSPITA
发明人： ABU-KHABAR KHALID S , WILLIAMS BRYAN R G , FREVEL MATHIAS , SILVERMAN ROBERT H
IPC分类号： C12N15/09 , C12M1/00 , C12M1/34 , C12N20060101 , C12Q1/68 , G01N33/483 , G06F19/20 , G06F19/22 , G06F19/28 , G06F19/00
CPC分类号： G06F19/28 , C12Q1/6897 , G06F19/20 , G06F19/22
摘要： Adenylate-uridylate-rich (ARE) elements present in the 3' untranslated region (UTR) of gene and mRNA sequences are disclosed. THE ARE motif comprises a sequence encompassed by: SEQ ID NO: 1 or 2, with further limitations. Computational methods of identifying genes or coding sequences in a database which comprise ARE elements are disclosed. The computational methods can be used for gene discovery, and sequence analysis. Methods of identifying, isolating and amplyfying ARE-element-associated polynucleotides using PCR, RT-PCR, hybridization, etc. are disclosed. PCR primers, oligonucleotide arrays, polynucleotide libraries, computer programs, and computer systems relating to ARE elements are disclosed.

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